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  1. Isolation of mast cells from the peritoneal exudate of the teleost fish gilthead sea bream (Sparus aurata L.).

    Fish & Shellfish Immunology 40(1):225 (2014) PMID 25047357

    We report a separation protocol for obtaining highly enriched (over 95% purity) preparations of fish mast cells in high numbers (5-20 million mast cells per fish). The peritoneal exudate of S. aurata is composed of lymphocytes, acidophilic granulocytes, macrophages and mast cells. We separated the l...
  2. TLR agonists extend the functional lifespan of professional phagocytic granulocytes in the bony fish gilthead seabream and direct precursor ...

    Molecular Immunology 48(6):846 (2011) PMID 21216011

    We examined the ability of toll-like receptor agonists to extend the lifespan of gilthead seabream ( Sparus aurata L.) acidophilic granulocytes, which are the functional equivalent of mammalian neutrophils. The results obtained demonstrated that apoptosis was also the default state of seabream...
  3. Regulation of immunity and disease resistance by commensal microbes and chromatin modifications during zebrafish development.

    PNAS 109(39):E2605 (2012) PMID 22949679 PMCID PMC3465450

    We show that colonization by commensals in newly hatched zebrafish primes neutrophils and induces several genes encoding proinflammatory and antiviral mediators, increasing the resistance of larvae to viral infection. Commensal microbe recognition was found to be mediated mainly through a TLR/MyD88...
  4. Professional phagocytic granulocytes of the bony fish gilthead seabream display functional adaptation to testicular microenvironment.

    Journal of Leukocyte Biology 78(2):345 (2005) PMID 15937143

    We show that phagocytosis and reactive oxygen intermediate (ROI) production were suppressed dramatically in testicular phagocytic granulocytes, compared with their level of activity in the head-kidney, the main hematopoietic organ in fish. Furthermore, testicular-conditioned media modulated migratio...
  5. Effects of recombinant flagellin B and its ND1 domain from Vibrio anguillarum on macrophages from gilthead seabream (Sparus aurata L.) and r...

    Fish & Shellfish Immunology 42(1):144 (2015) PMID 25449380

    We report the production of two recombinant proteins of Vibrio anguillarum: i) a full length flagellin B (FlaB) (rFla) and ii) the amino-terminus of the D1 domain (rND1) of the same protein, the region mainly responsible for binding to TLR5 and for the immunostimulatory activity of flagellin. The ef...
  6. Viral nervous necrosis virus persistently replicates in the central nervous system of asymptomatic gilthead seabream and promotes a transien...

    Developmental & Comparative Immunology 37(3-4):429 (2012) PMID 22402274

    We report that VNNV was able to replicate and persist for up to 3 months in the CNS of the gilthead seabream without causing any neural damage. In addition, we found an early inflammatory response in the CNS that was characterized by the induction of genes encoding pro-inflammatory cytokines, a dela...
  7. Characterization of macrophages from the bony fish gilthead seabream using an antibody against the macrophage colony-stimulating factor rece...

    Developmental & Comparative Immunology 32(10):1151 (2008) PMID 18420271

    We report here the generation of a monospecific rabbit polyclonal antibody against the gilthead seabream macrophage colony-stimulating factor receptor (Mcsfr), which is an excellent marker of macrophages in mammals and the zebrafish. The anti-Mcsfr has been found to be very useful in immunohistochem...
  8. Response to Boyle et al.

    Immunity 38(3):400 (2013) PMID 23521877

  9. TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3.

    Disease Models & Mechanisms 6(2):383 (2013) PMID 22956347 PMCID PMC3597020

    We show that targeted gene knockdown of TNFRSF1B in zebrafish embryos results in the induction of a caspase-8, caspase-2 and P53-dependent apoptotic program in endothelial cells that bypasses caspase-3. Furthermore, the simultaneous depletion of TNFRSF1A or the activation of NF-κB rescue endothelial...
  10. An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus.

    Virology Journal 10:188 (2013) PMID 23758704 PMCID PMC3691878

    We have expressed and purified the recombinant protein LDVICp016 and screened for potential interaction partners using surface plasmon resonance. Commercially available human and mouse members of the TNF superfamily (TNFSF), along with a representative set of fish-derived TNFSF were tested.We have f...