1. Role of histamine in the regulation of intestinal immunity in fish.

    Developmental & Comparative Immunology 64:178 (2016) PMID 26872545

    In mammals, during the acute inflammatory response, the complex interrelationship and cross-talk among histamine and the immune system has been fairly well characterized. There is a substantial body of information on its structure, metabolism, receptors, signal transduction, physiologic and path...
  2. TNFα Impairs Rhabdoviral Clearance by Inhibiting the Host Autophagic Antiviral Response.

    PLoS Pathogens 12(6):e1005699 (2016) PMID 27351838 PMCID PMC4924823

    TNFα is a pleiotropic pro-inflammatory cytokine with a key role in the activation of the immune system to fight viral infections. Despite its antiviral role, a few viruses might utilize the host produced TNFα to their benefit. Some recent reports have shown that anti-TNFα therapies could be util...
  3. Correction: Cutting Edge: TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.

    Journal of Immunology 196(8):3494 (2016) PMID 27044636

  4. Editorial: Fish neutrophils meet proresolving eicosanoids.

    Journal of Leukocyte Biology 99(2):227 (2016) PMID 26830729

  5. TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.

    Journal of Immunology 196(2):738 (2016) PMID 26673139

    As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos. Mechanistic...
  6. Neutrophils mediate Salmonella Typhimurium clearance through the GBP4 inflammasome-dependent production of prostaglandins.

    Nature Communications 7:12077 (2016) PMID 27363812 PMCID PMC4932187

    Inflammasomes are cytosolic molecular platforms that alert the immune system about the presence of infection. Here we report that zebrafish guanylate-binding protein 4 (Gbp4), an IFNγ-inducible GTPase protein harbouring a C-terminal CARD domain, is required for the inflammasome-dependent clearan...
  7. Estrogen receptor 2b deficiency impairs the antiviral response of zebrafish.

    Developmental & Comparative Immunology 53(1):55 (2015) PMID 26133072

    Although several studies have demonstrated the ability of some endocrine disruptive chemicals (EDCs) to alter the physiology of zebrafish, the immune-reproductive interaction has received little attention in this species. In this study, we used a homozygous line carrying an insertion of 8 amino ...
  8. A synthetic peptide derived from the D1 domain of flagellin induced the expression of proinflammatory cytokines in fish macrophages.

    Fish & Shellfish Immunology 47(1):239 (2015) PMID 26363237

    Flagellin is the main protein component of flagellum in Gram negative and positive bacteria, and it is also the ligand that activates the Toll-like receptor 5 (TLR5) in fish and mammals. In higher vertebrates, flagellin induces the activation of the membrane-bound TLR5 (TLR5M), which promotes th...
  9. Professional phagocytic granulocyte-derived PGD2 regulates the resolution of inflammation in fish.

    Developmental & Comparative Immunology 52(2):182 (2015) PMID 26027798

    Prostaglandins (PGs) play a key role in the development on the immune response through the regulation of both pro- and anti-inflammatory processes. PGD(2) can be either pro- or anti-inflammatory depending on the inflammatory milieu. Prostaglandin D synthase (PGDS) is the enzyme responsible for t...
  10. Md1 and Rp105 regulate innate immunity and viral resistance in zebrafish.

    Developmental & Comparative Immunology 50(2):155 (2015) PMID 25681741

    TLR4 was the first TLR family member identified in mammals and is responsible for the activation of the immune response by bacterial LPS. Later, MD1 and RP105 were shown to form complexes that directly interact with the MD2-TLR4 complex, acting as physiological negative regulators of LPS signali...
  11. Cxcl8-l1 and Cxcl8-l2 are required in the zebrafish defense against Salmonella Typhimurium.

    Developmental & Comparative Immunology 49(1):44 (2015) PMID 25445910

    In recent years zebrafish has emerged as an excellent model for studying the Cxcl8 signaling pathway in inflammation elicited upon tissue damage or infection. Zebrafish has two true homologs of mammalian CXCL8, named Cxcl8-l1 and Cxcl8-l2. Previously, we have shown that in wound-associated infla...
  12. Duox1-derived H2O2 modulates Cxcl8 expression and neutrophil recruitment via JNK/c-JUN/AP-1 signaling and chromatin modifications.

    Journal of Immunology 194(4):1523 (2015) PMID 25582859

    DUOX1-derived hydrogen peroxide (H2O2) and CXCL8 are two key neutrophil chemoattractants. H2O2 is critical at the early phase, whereas CXCL8 plays a key role in the late phases of recruitment, but the crosstalks between the two phases in vivo remain unknown. In this study using zebrafish, we rep...
  13. Selective Manipulation of the Gut Microbiota Improves Immune Status in Vertebrates.

    Frontiers in Immunology 6:512 (2015) PMID 26500650 PMCID PMC4598590

    All animals develop in association with complex microbial communities. It is now well established that commensal microbiota is essential for the correct functionality of each organ in the host. Particularly, the commensal gastro-intestinal microbiota (CGIM) is a key factor for development, immun...
  14. Effects of recombinant flagellin B and its ND1 domain from Vibrio anguillarum on macrophages from gilthead seabream (Sparus aurata L.) and rainbow trout (Oncorhynchus mykiss, W.).

    Fish & Shellfish Immunology 42(1):144 (2015) PMID 25449380

    Flagellin is the principal component of flagellum in Gram negative and positive bacteria, and it is also the ligand that activates the Toll-like receptor 5 (TLR5) in mammals and fish. In higher vertebrates, flagellin induces the activation of the membrane-bound TLR5 (TLR5M), which promotes the e...
  15. Proinflammatory signaling regulates hematopoietic stem cell emergence.

    Cell 159(5):1070 (2014) PMID 25416946 PMCID PMC4243083

    Hematopoietic stem cells (HSCs) underlie the production of blood and immune cells for the lifetime of an organism. In vertebrate embryos, HSCs arise from the unique transdifferentiation of hemogenic endothelium comprising the floor of the dorsal aorta during a brief developmental window. To date...
  16. Flagellin from Marinobacter algicola and Vibrio vulnificus activates the innate immune response of gilthead seabream.

    Developmental & Comparative Immunology 47(1):160 (2014) PMID 25020195

    Adjuvants have emerged as the best tools to enhance the efficacy of vaccination. However, the traditional adjuvants used in aquaculture may cause adverse alterations in fish making necessary the development of new adjuvants able to stimulate the immune system and offer strong protection against ...
  17. Histamine regulates the inflammatory response of the tunicate Styela plicata.

    Developmental & Comparative Immunology 46(2):382 (2014) PMID 24892817

    Histamine is stored inside hemocytes of the tunicate Styela plicata (Chordata, Tunicata, Ascidiacea), but no evidence on its role in the regulation of the immune response of this species has been reported. We examined whether histamine participated in the regulation of inflammation and host defe...
  18. Isolation of mast cells from the peritoneal exudate of the teleost fish gilthead sea bream (Sparus aurata L.).

    Fish & Shellfish Immunology 40(1):225 (2014) PMID 25047357

    Inflammation is the first response of animals to infection or tissue damage. Sparus aurata (Perciformes) was the first fish species shown to possess histamine-containing mast cells at mucosal tissues. We report a separation protocol for obtaining highly enriched (over 95% purity) preparations of...
  19. Proinflammatory signaling is required for hematopoietic stem cell emergence

    Experimental Hematology 42(8):S6 (2014)

  20. Fish granulocytes express a constitutively active androgen receptor variant

    Developmental & Comparative Immunology 45(1):115 (2014) PMID 24509065

    • A constitutively active AR variant, ARΔLBD, was identified in gilthead seabream. • ARΔLBD expression is regulated by testosterone in reproductive and immune orga...