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  1. Flagellin from Marinobacter algicola and Vibrio vulnificus activates the innate immune response of gilthead seabream.

    Developmental & Comparative Immunology 47(1):160 (2014) PMID 25020195

    We have evaluated the ability of recombinant flagellin from Marinobacter algicola (MA) and Vibrio vulnificus (Vvul), a non-pathogenic and a pathogenic bacteria, respectively, to stimulate the innate immune system of gilthead seabream (Sparus aurata L.) and compare the effect with that of the classic...
  2. Response to Boyle et al.

    Immunity 38(3):400 (2013) PMID 23521877

  3. Evolution of inflammasome functions in vertebrates: Inflammasome and caspase-1 trigger fish macrophage cell death but are dispensable for th...

    Innate Immunity 18(6):815 (2012) PMID 22456941

    We report here that stimulation or infection of seabream macrophages (MØ) led to the caspase-1-independent processing and release of IL-1β. In addition, several classical activators of the NLRP3 inflammasome failed to activate caspase-1 and to induce the processing and release of IL-1β. Furthermore,...
  4. Effects of in vitro addition of exogenous vitamins C and E on gilthead seabream (Sparus aurataL.) phagocytes

    Veterinary Immunology and Immunopathology 66(2):185 (1998)

    We report the in vitro effect of vitamin C and/or E on the functions and ultrastructure of gilthead seabream ( Sparus aurata L.) head–kidney phagocytic leukocytes. Head-kidney leukocytes were incubated with different concentrations of vitamins C and/or E for 48 h and then their migration...
  5. Profiling of infection specific mRNA transcripts of the European seabass Dicentrarchus labrax.

    BMC Genomics 10:157 (2009) PMID 19361338 PMCID PMC2674461

    We describe the construction, analysis and comparison of a total of ten cDNA libraries, six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen,...
  6. An overview of cell renewal in the testis throughout the reproductive cycle of a seasonal breeding teleost, the gilthead seabream (Sparus au...

    Biology of Reproduction 72(3):593 (2005) PMID 15548730

    We have previously described how acidophilic granulocytes infiltrate the testis during postspawning where, surprisingly, they produce interleukin-1beta, a known growth factor for mammalian spermatogonia, rather than being directly involved in the elimination of degenerative germ cells. In this study...
  7. TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3.

    Disease Models & Mechanisms 6(2):383 (2013) PMID 22956347 PMCID PMC3597020

    We show that targeted gene knockdown of TNFRSF1B in zebrafish embryos results in the induction of a caspase-8, caspase-2 and P53-dependent apoptotic program in endothelial cells that bypasses caspase-3. Furthermore, the simultaneous depletion of TNFRSF1A or the activation of NF-κB rescue endothelial...
  8. An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus.

    Virology Journal 10:188 (2013) PMID 23758704 PMCID PMC3691878

    We have expressed and purified the recombinant protein LDVICp016 and screened for potential interaction partners using surface plasmon resonance. Commercially available human and mouse members of the TNF superfamily (TNFSF), along with a representative set of fish-derived TNFSF were tested.We have f...
  9. Cxcl8-l1 and Cxcl8-l2 are required in the zebrafish defense against Salmonella Typhimurium.

    Developmental & Comparative Immunology 49(1):44 (2015) PMID 25445910

    We have shown that in wound-associated inflammation, these chemokines are up-regulated and are relevant for neutrophil recruitment. In infections, no such knowledge is available as most studies performed on this subject in zebrafish have mainly focused on Cxcl8-l1 even though Cxcl8-l2 shares higher...
  10. Evolution of lipopolysaccharide (LPS) recognition and signaling: fish TLR4 does not recognize LPS and negatively regulates NF-kappaB activat...

    Journal of Immunology 182(4):1836 (2009) PMID 19201835

    We think that the identification of TLR4 as a negative regulator of TLR signaling in the zebrafish, together with the absence of this receptor in most fish species, explains the resistance of fish to endotoxic shock and supports the idea that the TLR4 receptor complex for LPS recognition arose after...