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  1. Response to Boyle et al.

    Immunity 38(3):400 (2013) PMID 23521877

  2. Evolution of inflammasome functions in vertebrates: Inflammasome and caspase-1 trigger fish macrophage cell death but are dispensable for th...

    Innate Immunity 18(6):815 (2012) PMID 22456941

    We report here that stimulation or infection of seabream macrophages (MØ) led to the caspase-1-independent processing and release of IL-1β. In addition, several classical activators of the NLRP3 inflammasome failed to activate caspase-1 and to induce the processing and release of IL-1β. Furthermore,...
  3. Effects of in vitro addition of exogenous vitamins C and E on gilthead seabream (Sparus aurataL.) phagocytes

    Veterinary Immunology and Immunopathology 66(2):185 (1998)

    We report the in vitro effect of vitamin C and/or E on the functions and ultrastructure of gilthead seabream ( Sparus aurata L.) head–kidney phagocytic leukocytes. Head-kidney leukocytes were incubated with different concentrations of vitamins C and/or E for 48 h and then their migration...
  4. Evolution of lipopolysaccharide (LPS) recognition and signaling: fish TLR4 does not recognize LPS and negatively regulates NF-kappaB activat...

    Journal of Immunology 182(4):1836 (2009) PMID 19201835

    We think that the identification of TLR4 as a negative regulator of TLR signaling in the zebrafish, together with the absence of this receptor in most fish species, explains the resistance of fish to endotoxic shock and supports the idea that the TLR4 receptor complex for LPS recognition arose after...
  5. Molecular characterization of interleukin-6 in the gilthead seabream (Sparus aurata).

    Molecular Immunology 45(12):3363 (2008) PMID 18513800

    A cDNA clone, designated sbIL-6 (seabream interleukin-6), was obtained from a cDNA library of enriched immune-stimulated sequences from gilthead seabream. The deduced sbIL-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and...
  6. Molecular and functional characterization of gilthead seabream Sparus aurata caspase-1: the first identification of an inflammatory caspase ...

    Molecular Immunology 45(1):49 (2008) PMID 17610954

    We cloned a caspase-1-like gene from the bony fish gilthead seabream (Sparus aurata L.) which shows a conserved N-terminal caspase-recruitment domain (CARD) and a C-terminal caspase catalytic domain. The seabream caspase-1 gene was expressed in 1 day post-hatching larvae and its mRNA levels increase...
  7. Toll-like receptor 22 of gilthead seabream, Sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation.

    Developmental & Comparative Immunology 44(1):173 (2014) PMID 24333435

    TLR22 is a fish-specific TLR that recognizes dsRNAs. In the present study, a TLR22 homologue gene from gilthead seabream (sbTLR22) was identified and characterized. The full coding sequence contained a single open-reading frame of 2895 nucleotides encoding a predicted protein of 964 amino acids in l...
  8. Evolution of the inflammatory response in vertebrates: fish TNF-alpha is a powerful activator of endothelial cells but hardly activates phag...

    Journal of Immunology 181(7):5071 (2008) PMID 18802111

    We report here that the main proinflammatory effects of fish TNF-alpha are mediated through the activation of endothelial cells. Thus, TNF-alpha promotes the expression of E-selectin and different CC and CXC chemokines in endothelial cells, thus explaining the recruitment and activation of phagocyte...
  9. TNF receptors regulate vascular homeostasis in zebrafish through a caspase-8, caspase-2 and P53 apoptotic program that bypasses caspase-3.

    Disease Models & Mechanisms 6(2):383 (2013) PMID 22956347 PMCID PMC3597020

    We show that targeted gene knockdown of TNFRSF1B in zebrafish embryos results in the induction of a caspase-8, caspase-2 and P53-dependent apoptotic program in endothelial cells that bypasses caspase-3. Furthermore, the simultaneous depletion of TNFRSF1A or the activation of NF-κB rescue endothelial...
  10. An orphan viral TNF receptor superfamily member identified in lymphocystis disease virus.

    Virology Journal 10:188 (2013) PMID 23758704 PMCID PMC3691878

    We have expressed and purified the recombinant protein LDVICp016 and screened for potential interaction partners using surface plasmon resonance. Commercially available human and mouse members of the TNF superfamily (TNFSF), along with a representative set of fish-derived TNFSF were tested.We have f...