1. Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall.

    Journal of Immunology 195(3):1006 (2015) PMID 26101322

    Targeting Ags to dendritic cell (DC) surface receptors can induce a variety of responses depending on the DC type targeted, the receptor targeted, and the adjuvant used. Clec9A (DNGR-1), which is expressed by CD8(+) DCs, has been shown to bind F-actin exposed on damaged cells. Targeting Ag to th...
  2. Krüppel-ling of IRF4-Dependent DCs into Two Functionally Distinct DC Subsets.

    Immunity 42(5):785 (2015) PMID 25992853

    IRF4-dependent DCs have been associated with induction of both Th1 and Th17 cells. In this issue of Immunity, Tussiwand et al. (2015) demonstrate that a dependence on KLF4 identifies a subset of IRF4-dependent DC that preferentially promotes Th2 cell differentiation. Copyright © 2015 Elsevier In...
  3. Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates.

    European Journal of Immunology 45(3):854 (2015) PMID 25487143

    Targeting antigens to dendritic cell (DC) surface receptors using antibodies has been successfully used to generate strong immune responses and is currently in clinical trials for cancer immunotherapy. Whilst cancer immunotherapy focuses on the induction of CD8(+) T-cell responses, many successf...
  4. Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates.

    European Journal of Immunology 45(3):854 (2015) PMID 25487143

    Targeting antigens to dendritic cell (DC) surface receptors using antibodies has been successfully used to generate strong immune responses and is currently in clinical trials for cancer immunotherapy. Whilst cancer immunotherapy focuses on the induction of CD8(+) T-cell responses, many successf...
  5. Intrahepatic activation of naive CD4+ T cells by liver-resident phagocytic cells.

    Journal of Immunology 193(5):2087 (2014) PMID 25070847

    Naive T cell activation is normally restricted to the lymphoid organs, in part because of their limited ability to migrate into the parenchyma of peripheral tissues. The liver vasculature is unique, however, and circulating leukocytes within the hepatic sinusoids have direct access to liver-resi...
  6. Intrahepatic activation of naive CD4+ T cells by liver-resident phagocytic cells.

    Journal of Immunology 193(5):2087 (2014) PMID 25070847

    Naive T cell activation is normally restricted to the lymphoid organs, in part because of their limited ability to migrate into the parenchyma of peripheral tissues. The liver vasculature is unique, however, and circulating leukocytes within the hepatic sinusoids have direct access to liver-resi...
  7. Distinct APC subtypes drive spatially segregated CD4+ and CD8+ T-cell effector activity during skin infection with HSV-1.

    PLoS Pathogens 10(8):e1004303 (2014) PMID 25121482 PMCID PMC4133397

    Efficient infection control requires potent T-cell responses at sites of pathogen replication. However, the regulation of T-cell effector function in situ remains poorly understood. Here, we show key differences in the regulation of effector activity between CD4+ and CD8+ T-cells during skin inf...
  8. Distinct APC subtypes drive spatially segregated CD4+ and CD8+ T-cell effector activity during skin infection with HSV-1.

    PLoS Pathogens 10(8):e1004303 (2014) PMID 25121482 PMCID PMC4133397

    Efficient infection control requires potent T-cell responses at sites of pathogen replication. However, the regulation of T-cell effector function in situ remains poorly understood. Here, we show key differences in the regulation of effector activity between CD4+ and CD8+ T-cells during skin inf...
  9. CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria.

    PLoS Pathogens 10(5):e1004135 (2014) PMID 24854165 PMCID PMC4031232

    To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent mala...
  10. CD8+ T cells from a novel T cell receptor transgenic mouse induce liver-stage immunity that can be boosted by blood-stage infection in rodent malaria.

    PLoS Pathogens 10(5):e1004135 (2014) PMID 24854165 PMCID PMC4031232

    To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent mala...
  11. Persistence of skin-resident memory T cells within an epidermal niche.

    PNAS 111(14):5307 (2014) PMID 24706879 PMCID PMC3986170

    Barrier tissues such as the skin contain various populations of immune cells that contribute to protection from infections. These include recently identified tissue-resident memory T cells (TRM). In the skin, these memory CD8(+) T cells reside in the epidermis after being recruited to this site ...
  12. Persistence of skin-resident memory T cells within an epidermal niche.

    PNAS 111(14):5307 (2014) PMID 24706879 PMCID PMC3986170

    Barrier tissues such as the skin contain various populations of immune cells that contribute to protection from infections. These include recently identified tissue-resident memory T cells (TRM). In the skin, these memory CD8(+) T cells reside in the epidermis after being recruited to this site ...
  13. The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.

    Nature Immunology 14(12):1294 (2013) PMID 24162776

    Tissue-resident memory T cells (T(RM) cells) provide superior protection against infection in extralymphoid tissues. Here we found that CD103(+)CD8(+) T(RM) cells developed in the skin from epithelium-infiltrating precursor cells that lacked expression of the effector-cell marker KLRG1. A combin...
  14. The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.

    Nature Immunology 14(12):1294 (2013) PMID 24162776

    Tissue-resident memory T cells (T(RM) cells) provide superior protection against infection in extralymphoid tissues. Here we found that CD103(+)CD8(+) T(RM) cells developed in the skin from epithelium-infiltrating precursor cells that lacked expression of the effector-cell marker KLRG1. A combin...
  15. The skin-resident and migratory immune system in steady state and memory: innate lymphocytes, dendritic cells and T cells.

    Nature Immunology 14(10):978 (2013) PMID 24048119

    The skin is a highly complex organ interspersed with a variety of smaller organ-like structures and a plethora of cell types that together perform essential functions such as physical sensing, temperature control, barrier maintenance and immunity. In this Review, we outline many of the innate an...
  16. The skin-resident and migratory immune system in steady state and memory: innate lymphocytes, dendritic cells and T cells.

    Nature Immunology 14(10):978 (2013) PMID 24048119

    The skin is a highly complex organ interspersed with a variety of smaller organ-like structures and a plethora of cell types that together perform essential functions such as physical sensing, temperature control, barrier maintenance and immunity. In this Review, we outline many of the innate an...
  17. Distinct resident and recirculating memory T cell subsets in non-lymphoid tissues.

    Current Opinion in Immunology 25(3):329 (2013) PMID 23746791

    Antigen experienced or memory T cells make a critical contribution to immunity against infection. Many pathogens colonise non-lymphoid tissues and memory T cells in these compartments can deal with such localised infections. Emerging data show that there are at least two phenotypically distinct ...
  18. Distinct resident and recirculating memory T cell subsets in non-lymphoid tissues.

    Current Opinion in Immunology 25(3):329 (2013) PMID 23746791

    Antigen experienced or memory T cells make a critical contribution to immunity against infection. Many pathogens colonise non-lymphoid tissues and memory T cells in these compartments can deal with such localised infections. Emerging data show that there are at least two phenotypically distinct ...
  19. Distinct resident and recirculating memory T cell subsets in non-lymphoid tissues

    Current Opinion in Immunology 25(3):329 (2013)

    • Peripheral tissues contain a complex mixture of migrating and sessile memory T cells. • CD103+CD8+ memory T cells are the best characterised type of tissue-resid...
  20. Identification of a MHC I-restricted epitope of DsRed in C57BL/6 mice.

    Molecular Immunology 53(4):450 (2013) PMID 23142929

    Fluorescent proteins can be used to visualize cells and their constituents by various imaging techniques. Adoptive transfer of T cells from C57Bl/6 (B6) mice that expressed DsRed.T3 under the actin promoter lead to frequent rejection of transferred cells. In short term in vivo cytotoxicity assay...