Streptococcal serum opacity factor promotes cholesterol ester metabolism and bile acid secretion in vitro and in vivo.
Biochimica et Biophysica Acta 1861(3):196 (2016)
Plasma high density lipoprotein-cholesterol (HDL-C) concentrations negatively correlate with atherosclerotic cardiovascular disease. HDL is thought to have several atheroprotective functions, which are likely distinct from the epidemiological inverse relationship between HDL-C levels and risk. S...
Ca2+ Binding/Permeation via Calcium Channel, CaV1.1, Regulates the Intracellular Distribution of the Fatty Acid Transport Protein, CD36, and Fatty Acid Metabolism.
Journal of Biological Chemistry 290(39):23751 (2015)
Ca(2+) permeation and/or binding to the skeletal muscle L-type Ca(2+) channel (CaV1.1) facilitates activation of Ca(2+)/calmodulin kinase type II (CaMKII) and Ca(2+) store refilling to reduce muscle fatigue and atrophy (Lee, C. S., Dagnino-Acosta, A., Yarotskyy, V., Hanna, A., Lyfenko, A., Knobl...
Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model.
Nature Communications 6:7339 (2015)
Diseases of lipid metabolism are a major cause of human morbidity, but no animal model entirely recapitulates human lipoprotein metabolism. Here we develop a xenograft mouse model using hepatocytes from a patient with familial hypercholesterolaemia caused by loss-of-function mutations in the low...
Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species.
Lipids 49(6):505 (2014)
Hepatic lipase (HL) and endothelial lipase (EL) share overlapping and complementary roles in lipoprotein metabolism. The deletion of HL and EL alleles in mice raises plasma total cholesterol and phospholipid concentrations. However, the influence of HL and EL in vivo on individual molecular spec...
Genetic manipulation of the ApoF/Stat2 locus supports an important role for type I interferon signaling in atherosclerosis.
Atherosclerosis 233(1):234 (2014)
Apolipoprotein F (ApoF) is a sialoglycoprotein that is a component of the HDL and LDL fractions of human serum. We sought to test the hypothesis that ApoF plays an important role in atherosclerosis in mice by modulating lipoprotein function. Atherosclerosis was assessed in male low density lipop...
Adeno-associated viruses as liver-directed gene delivery vehicles: focus on lipoprotein metabolism.
Methods in Molecular Biology 1027:273 (2013)
Adeno-associated viral vectors have proven to be excellent gene delivery vehicles for somatic overexpression. These viral vectors can efficiently and selectively target the liver, which plays a central role in lipoprotein metabolism. Both liver-expressed as well as non-hepatic secreted proteins ...
The effects of apolipoprotein F deficiency on high density lipoprotein cholesterol metabolism in mice.
PLoS ONE 7(2):e31616 (2012)
Apolipoprotein F (apoF) is 29 kilodalton secreted sialoglycoprotein that resides on the HDL and LDL fractions of human plasma. Human ApoF is also known as Lipid Transfer Inhibitor protein (LTIP) based on its ability to inhibit cholesteryl ester transfer protein (CETP)-mediated transfer events be...
Thyroid hormone enhances the ability of serum to accept cellular cholesterol via the ABCA1 transporter.
Atherosclerosis 218(1):77 (2011)
The goal of this study was to examine the effects of thyroid hormone status on the ability of serum to accept cellular cholesterol.
Sera from hypophysectomized rats treated ± T(3) was used to evaluate the role of thyroid hormone on serum efflux capacity. 2D-DIGE analysis of serum proteins showed...
Overexpression of apolipoprotein O does not impact on plasma HDL levels or functionality in human apolipoprotein A-I transgenic mice.
Biochimica et Biophysica Acta 1811(4):294 (2011)
Apolipoprotein (apo) O is a newly discovered apolipoprotein preferentially contained within HDL; however, currently, no data are available on the (patho)physiological effects of apoO. Therefore, the present study assessed the impact of apoO overexpression on (i) plasma lipids and lipoproteins as...
Phospholipidation of HDL--how much is too much?
Clinical Chemistry 57(1):4 (2011)
Trib1 is a lipid- and myocardial infarction-associated gene that regulates hepatic lipogenesis and VLDL production in mice.
Journal of Clinical Investigation 120(12):4410 (2010)
Recent genome-wide association studies have identified a genetic locus at human chromosome 8q24 as having minor alleles associated with lower levels of plasma triglyceride (TG) and LDL cholesterol (LDL-C), higher levels of HDL-C, as well as decreased risk for myocardial infarction. This locus co...
Impact of combined deficiency of hepatic lipase and endothelial lipase on the metabolism of both high-density lipoproteins and apolipoprotein B-containing lipoproteins.
Circulation Research 107(3):357 (2010)
Hepatic lipase (HL) and endothelial lipase (EL) are extracellular lipases that both hydrolyze triglycerides and phospholipids and display potentially overlapping or complementary roles in lipoprotein metabolism.
We sought to dissect the overlapping roles of HL and EL by generating mice deficient...
Proteomic analysis of defined HDL subpopulations reveals particle-specific protein clusters: relevance to antioxidative function.
Arteriosclerosis, Thrombosis, and Vascular Biology 29(6):870 (2009)
Recent proteomic studies have identified multiple proteins that coisolate with human HDL. We hypothesized that distinct clusters of protein components may distinguish between physicochemically-defined subpopulations of HDL particles, and that such clusters may exert specific biological function(...
Technological advances in the study of HLA-DRA promoter regulation: extending the functions of CIITA, Oct-1, Rb, and RFX.
Acta Biochimica et Biophysica Sinica 41(3):198 (2009)
Several advances were established in examining the interaction of transcriptional factors with the HLA-DRA promoter. First, hydrodynamic injection was used to demonstrate the activation of the promoter by class II transactivator in a live mouse. Second, the Oct-1 DNA-binding site in the HLA-DRA ...
Overexpression of apolipoprotein F reduces HDL cholesterol levels in vivo.
Arteriosclerosis, Thrombosis, and Vascular Biology 29(1):40 (2009)
Apolipoprotein F (ApoF) is a protein component of several lipoprotein classes including HDL. It is also known as lipid transfer inhibitor protein (LTIP) based on its ability to inhibit lipid transfer between lipoproteins ex vivo. We sought to investigate the role of ApoF in HDL metabolism.
Functional analysis of the hepatic HMG-CoA reductase promoter by in vivo electroporation.
Experimental Biology and Medicine 232(3):353 (2007)
HMG-CoA reductase (HMGR) catalyzes the rate-controlling step in cholesterol production. This enzyme is highly expressed in the liver, where it is subject to extensive hormonal and dietary regulation. Although much is known about the regulation of the HMGR promoter in cultured cells, this issue h...
Diabetes alters the occupancy of the hepatic 3-hydroxy-3-methylglutaryl-CoA reductase promoter.
Journal of Biological Chemistry 280(44):36601 (2005)
Hepatic 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) protein and mRNA are substantially decreased in diabetic animals and rapidly restored by the administration of insulin. To begin to examine the underlying molecular mechanisms, measurements of transcription by nuclear run-on assays and an i...
Identification of insulin-responsive regions in the HMG-CoA reductase promoter.
Biochemical and Biophysical Research Communicat... 318(4):814 (2004)
An insulin-responsive line of rat hepatoma cells, H4IIE, was used to investigate the basis for insulin's transcriptional regulation of HMG-CoA reductase. Insulin addition to the media of these cells resulted in at least a 10-fold increase in levels of HMG-CoA reductase protein. Adding insulin to...
Identification of insulin-responsive regions in the HMG-CoA reductase promoter
Biochemical and Biophysical Research Communicat... 318(4):814 (2004)
An insulin-responsive line of rat hepatoma cells, H4IIE, was used to investigate the basis for insulin’s transcriptional regulation of HMG-CoA reductase. Insulin addition to the media of these cells resulted in at least a 10-fold increase in levels of HMG-CoA reductase protein. Adding insul...