1. AtPEPTIDE RECEPTOR2 mediates the AtPEPTIDE1-induced cytosolic Ca(2+) rise, which is required for the suppression of Glutamine Dumper gene expression in Arabidopsis roots.

    Journal of Integrative Plant Biology 56(7):684 (2014) PMID 24450616

    AtPEPTIDE RECEPTOR2 (AtPEPR2) is a member of leucine-rich repeat receptor-like kinase family and binds to a group of AtPROPEP gene-encoded endogenous peptides, AtPeps. Previously, we found that AtPEPR2 plays a moderate role in the AtPep1-mediated innate immunity responses in Arabidopsis leaf. In...
  2. Endogenous peptide elicitors in higher plants.

    Current Opinion in Plant Biology 14(4):351 (2011) PMID 21636314

    Plant defense responses against invading organisms are initiated through the perception of molecules associated with attacking microbes and herbivores by pattern recognition receptors. In addition to elicitor molecules derived from attacking organisms, plants recognize host-derived molecules. Th...
  3. GmPep914, an eight-amino acid peptide isolated from soybean leaves, activates defense-related genes.

    Plant Physiology 156(2):932 (2011) PMID 21478368 PMCID PMC3177287

    Only a handful of endogenous peptide defense signals have been isolated from plants. Herein, we report a novel peptide from soybean (Glycine max) leaves that is capable of alkalinizing the media of soybean suspension cells, a response that is generally associated with defense peptides. The pepti...
  4. Ca2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca2+ channels.

    PNAS 107(49):21193 (2010) PMID 21088220 PMCID PMC3000296

    A family of peptide signaling molecules (AtPeps) and their plasma membrane receptor AtPepR1 are known to act in pathogen-defense signaling cascades in plants. Little is currently known about the molecular mechanisms that link these signaling peptides and their receptor, a leucine-rich repeat rec...
  5. Structure-activity studies of RALF, Rapid Alkalinization Factor, reveal an essential--YISY--motif.

    Peptides 31(11):1973 (2010) PMID 20800638

    Rapid Alkalinization Factor (RALF) is a 49-amino acid peptide initially isolated from tobacco leaves that is capable of arresting both root and pollen tube growth. With suspension cells, addition of RALF causes an elevation of the pH of the extracellular media, caused by the blockage of a proton...
  6. A subtilisin-like protein from soybean contains an embedded, cryptic signal that activates defense-related genes.

    PNAS 107(33):14921 (2010) PMID 20679205 PMCID PMC2930467

    Among the arsenal of plant-derived compounds activated upon attack by herbivores and pathogens are small peptides that initiate and amplify defense responses. However, only a handful of plant signaling peptides have been reported. Here, we have isolated a 12-aa peptide from soybean (Glycine max)...
  7. PEPR2 is a second receptor for the Pep1 and Pep2 peptides and contributes to defense responses in Arabidopsis.

    Plant Cell 22(2):508 (2010) PMID 20179141 PMCID PMC2845411

    Pep1 is a 23-amino acid peptide that enhances resistance to a root pathogen, Pythium irregulare. Pep1 and its homologs (Pep2 to Pep7) are endogenous amplifiers of innate immunity of Arabidopsis thaliana that induce the transcription of defense-related genes and bind to PEPR1, a plasma membrane l...
  8. Structure–activity studies of GmSubPep, a soybean peptide defense signal derived from an extracellular protease

    Peptides 31(12):2159 (2010) PMID 20833217

    Research highlights ▶ Substitutions of alanine at each of the 12 amino acid positions revealed that the amino acids at positions 10 (arginine) and 12 (histidine) were essential for activity. ▶ Both analogs, [A 10]GmSubPep and [A 12]GmSubPep were able to reduce the physiolog...
  9. Structure–activity studies of GmSubPep, a soybean peptide defense signal derived from an extracellular protease

    Peptides 31(12):2159 (2010) PMID 20833217

    Research highlights ▶ Substitutions of alanine at each of the 12 amino acid positions revealed that the amino acids at positions 10 (arginine) and 12 (histidine) were essential for activity. ▶ Both analogs, [A 10]GmSubPep and [A 12]GmSubPep were able to reduce the physiolog...
  10. Isolation and characterization of hydroxyproline-rich glycopeptide signals in black nightshade leaves.

    Plant Physiology 150(3):1422 (2009) PMID 19403725 PMCID PMC2705048

    A gene encoding a preprohydroxyproline-rich systemin, SnpreproHypSys, was identified from the leaves of black nightshade (Solanum nigrum), which is a member of a small gene family of at least three genes that have orthologs in tobacco (Nicotiana tabacum; NtpreproHypSys), tomato (Solanum lycopers...
  11. Structure-activity studies of AtPep1, a plant peptide signal involved in the innate immune response.

    Peptides 29(12):2083 (2008) PMID 18824048

    AtPep1, a 23-amino acid peptide recently isolated from Arabidopsis leaves, induces the expression of the genes encoding defense proteins against pathogens. We investigated the structure-activity relationship of AtPep1 with its receptor, a 170 kDa leucine-rich repeat receptor kinase (AtPEPR1) by ...
  12. New insights into innate immunity in Arabidopsis.

    Cellular Microbiology 9(8):1902 (2007) PMID 17593247

    The term innate immunity has been described as '. . . the surveillance system that detects the presence and nature of the infection and provides the first line of host defense . . .' (Medzhitov, 2001; Nat Rev Immunol 1: 135-145). The strategy of innate immunity is based on the recognition of con...
  13. The cell surface leucine-rich repeat receptor for AtPep1, an endogenous peptide elicitor in Arabidopsis, is functional in transgenic tobacco cells.

    PNAS 103(26):10104 (2006) PMID 16785433 PMCID PMC1502513

    AtPep1 is a 23-aa endogenous peptide elicitor from Arabidopsis leaves that signals the activation of components of the innate immune response against pathogens. Here, we report the isolation of an AtPep1 receptor from the surface of Arabidopsis suspension-cultured cells. An (125)I-labeled AtPep1...
  14. Direct interaction between the tobacco mosaic virus helicase domain and the ATP-bound resistance protein, N factor during the hypersensitive response in tobacco plants.

    Plant Molecular Biology 61(1-2):31 (2006) PMID 16786290

    Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not complete...
  15. Activation of a novel transcription factor through phosphorylation by WIPK, a wound-induced mitogen-activated protein kinase in tobacco plants.

    Plant Physiology 139(1):127 (2005) PMID 16113214 PMCID PMC1203363

    Wound-induced protein kinase (WIPK) is a tobacco (Nicotiana tabacum) mitogen-activated protein kinase known to play an essential role in defense against wounding and pathogens, although its downstream targets have yet to be clarified. This study identified a gene encoding a protein of 648 amino ...
  16. A hypersensitive response-induced ATPase associated with various cellular activities (AAA) protein from tobacco plants.

    Plant Molecular Biology 56(6):973 (2004) PMID 15821994

    The hypersensitive response (HR) is one of the most critical defense systems in higher plants. In order to understand its molecular basis, we have screened tobacco genes that are transcriptionally activated during the early stage of the HR by the differential display method. Among six genes init...
  17. Role of a nonselective de novo DNA methyltransferase in maternal inheritance of chloroplast genes in the green alga, Chlamydomonas reinhardtii.

    Genetics 168(2):809 (2004) PMID 15514055 PMCID PMC1448840

    In the green alga, Chlamydomonas, chloroplast DNA is maternally transmitted to the offspring. We previously hypothesized that the underlying molecular mechanism involves specific methylation of maternal gamete DNA before mating, protecting against degradation. To obtain direct evidence for this,...
  18. Induction of transcripts encoding a novel seven-transmembrane protein during the hypersensitive response to tobacco mosaic virus infection in tobacco plants.

    Planta 218(2):249 (2003) PMID 13680232

    Immediate early responsive genes were screened by the differential display method during the hypersensitive response upon tobacco mosaic virus infection of tobacco ( Nicotiana tabacum L.) plants carrying the N gene. Three hours after temperature shift from 30 degrees C to 20 degrees C, an increa...
  19. Preferential de novo methylation of cytosine residues in non-CpG sequences by a domains rearranged DNA methyltransferase from tobacco plants.

    Journal of Biological Chemistry 278(43):42386 (2003) PMID 12917429

    In plant DNA, cytosines in symmetric CpG and CpNpG (N is A, T, or C) are thought to be methylated by DNA methyltransferases, MET1 and CMT3, respectively. Cytosines in asymmetric CpNpN are also methylated, and genetic analysis has suggested the responsible enzyme to be domains rearranged methyltr...
    PDF not found
  20. Induction of hypersensitive cell death by hydrogen peroxide produced through polyamine degradation in tobacco plants.

    Plant Physiology 132(4):1973 (2003) PMID 12913153 PMCID PMC181282

    Screening immediate-early responding genes during the hypersensitive response (HR) against tobacco mosaic virus infection in tobacco (Nicotiana tabacum) plants, we identified a gene encoding ornithine decarboxylase. Subsequent analyses showed that other genes involved in polyamine biosynthesis w...