1. Ligand-Dependent Enhancer Activation Regulated by Topoisomerase-I Activity

    Cell 163(2):520 (2015)

  2. LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control.

    Nature Neuroscience 18(9):1256 (2015) PMID 26214369 PMCID PMC4625987

    We found that a neuron-specific isoform of LSD1, LSD1n, which results from an alternative splicing event, acquires a new substrate specificity, targeting histone H4 Lys20 methylation, both in vitro and in vivo. Selective genetic ablation of LSD1n led to deficits in spatial learning and memory, r...
  3. Enhancer-bound LDB1 regulates a corticotrope promoter-pausing repression program.

    PNAS 112(5):1380 (2015) PMID 25605944 PMCID PMC4321301

    Substantial evidence supports the hypothesis that enhancers are critical regulators of cell-type determination, orchestrating both positive and negative transcriptional programs; however, the basic mechanisms by which enhancers orchestrate interactions with cognate promoters during activation an...
  4. Ligand-dependent enhancer activation regulated by topoisomerase-I activity.

    Cell 160(3):367 (2015) PMID 25619691 PMCID PMC4422651

    The discovery that enhancers are regulated transcription units, encoding eRNAs, has raised new questions about the mechanisms of their activation. Here, we report an unexpected molecular mechanism that underlies ligand-dependent enhancer activation, based on DNA nicking to relieve torsional stre...
  5. Tyrosine phosphorylation of histone H2A by CK2 regulates transcriptional elongation.

    Nature 516(7530):267 (2014) PMID 25252977 PMCID PMC4461219

    Post-translational histone modifications have a critical role in regulating transcription, the cell cycle, DNA replication and DNA damage repair. The identification of new histone modifications critical for transcriptional regulation at initiation, elongation or termination is of particular inte...
  6. PRB1 is required for clipping of the histone H3 N terminal tail in Saccharomyces cerevisiae.

    PLoS ONE 9(2):e90496 (2014) PMID 24587380 PMCID PMC3938757

    Cathepsin L, a lysosomal protein in mouse embryonic stem cells has been shown to clip the histone H3 N- terminus, an activity associated with gene activity during mouse cell development. Glutamate dehydrogenase (GDH) was also identified as histone H3 specific protease in chicken liver, which has...
  7. Acetylated histone H3K56 interacts with Oct4 to promote mouse embryonic stem cell pluripotency.

    PNAS 110(28):11493 (2013) PMID 23798425 PMCID PMC3710873

    The presence of acetylated histone H3K56 (H3K56ac) in human ES cells (ESCs) correlates positively with the binding of Nanog, Sox2, and Oct4 (NSO) transcription factors at their target gene promoters. However, the function of H3K56ac there has been unclear. We now report that Oct4 interacts with ...
  8. Estimating the quality of reprogrammed cells using ES cell differentiation expression patterns.

    PLoS ONE 6(1):e15336 (2011) PMID 21283513 PMCID PMC3023460

    Somatic cells can be reprogrammed to a pluripotent state by over-expression of defined factors, and pluripotency has been confirmed by the tetraploid complementation assay. However, especially in human cells, estimating the quality of Induced Pluripotent Stem Cell(iPSC) is still difficult. Here,...
  9. Transcriptional inhibiton of Hoxd4 expression by miRNA-10a in human breast cancer cells.

    BMC Molecular Biology 10:12 (2009) PMID 19232136 PMCID PMC2680403

    Small noncoding RNAs (ncRNAs), including short interfering RNAs (siRNAs) and microRNAs (miRNAs), can silence genes at the transcriptional, post-transcriptional or translational level 12. Here, we show that microRNA-10a (miR-10a) targets a homologous DNA region in the promoter region of the hoxd4...