Acta Microbiologica Sinica
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[Genetic diversity of rhizobial bacteria nodulating Tibetia himalaica in eastern Qinghai-Tibet plateau].
Genetic diversity of the rhizobial bacteria nodulating Tibetia himalaica in the eastern part of Qinghai-Tibet Plateau (Ganzi State, Sichuan) were evaluated. The pure culture method was used for isolating the rhiobial strains from the nodules. BOXAIR, 16S rDNA sequences, 16S rDNA PCR-RFLP and Princip...
[Screening and identification of glycoproteins from Campylobacter jejuni NCTC 11168 based on specific affinity between lectins and glycoprot...
We introduced a screening strategy based on specific affinity between lectins and glycoproteins. First, the specific affinity between Soybean Agglutinin (SBA) and glycoproteins in C. jejuni was confirmed by western blotting. Then, magnetic beads coating with Soybean Agglutinin (SBA) were used to cap...
[Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes].
To study the effect of RsbV gene deletion on the virulence of Listeria monocytogenes. The fragment with RsbV deletion was generated by gene overlap extension PCR (SOE-PCR) and the mutant with RsbV deletion was obtained by homologous recombination based on the wild strain LM-XS5. The differences in v...
[Effect of aromatic amino acid transport gene knock-out on L-tryptophan accumulation in Corynebacterium pekinense PD-67].
A Corynebacterium pekinense PD-67 mutant with aromatic amino acid transport system gene (aroP) in-frame deletion was constructed to decrease the uptake of L-tryptophan and reduce the intracellular pool of L-tryptophan, further to deregulate the feedback regulation of L-tryptophan and increase the ex...
[CO2 sequestration coupled with industrial cultivation of microalgae].
In order to reduce CO2 emission and lower the cost of microalgal industrial production at the same time, a technology was developed to combine CO2 sequestration with microalgal cultivation. The technology was based on rapid pH drift and high pH adaptability of two microalgal species, Chlorococcum al...
[Thermotolerance of Bacillus licheniformis CGMCC 3963 from high-temperature Daqu].
We determined the thermotolerance of Bacillus licheniformis CGMCC 3963, and analyzed cell morphology, genomics and transcriptomics. B. licheniformis CGMCC 3963 could survive at 55 degrees C and produce many capsules. Genes of class I heat-shock proteins and polyglutamate biosynthesis...
[Calcium homeostasis systems and calcium signaling pathways in Candida albicans--a review].
Candida albicans is one of the most important conditional pathogens. Calcium homeostasis systems and calcium signaling in this pathogen are closely associated with numerous physiological processes, such as pathogenesis and stress responses. Study on calcium homeostasis systems and ca...
[Capsular saliva acid of Streptococcus suis 2 influences virulence and host inflammatory responses].
We clarified the pathogenic influence of the absence of Streptococcus suis type 2 capsular saliva acid on BLAB/c mice. The virulence of the experimental strains were compared; the distribution of strains in vivo was determined by quantitative plating. Histopathological analysis was u...
[Construction of BV-T7 hybrid expression system based on baculovirus to express target gene eGFP in mammalian and chicken cells].
We established a hybrid baculovirus-T7 RNA polymerase system for transient expression in mammalian cells and chicken cells. Two recombinant baculoviruses were constructed, one carrying cDNA of bacteriophage T7 RNA polymerase, with a nuclear localization signal, under the control of a mammalian promo...
[Community structure of ammonia-oxidizing prokaryotes at the dry-up lake in Huitengxile grassland].
To investigate the structure of ammonia-oxidation microbial communities in the wetlands to dry-up process at 99 degraded lakes of the Huitengxile grassland in the Inner Mongolia Plateau.