Analyst (Cambridge UK)
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A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor.
We demonstrated a simple and highly sensitive LED-induced chemiluminescence (LED-CL) platform for aptasensing of proteins. It was based on the fact that fluorescein isothiocyanate (FITC) strongly catalyzed the reaction between luminol and dissolved oxygen under LED irradiation. Using FITC as a new c...
LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation.
Self-aggregation of amyloid-β (Aβ) plays an important role in the pathogenesis of Alzheimer's disease (AD). Small molecule inhibitors of Aβ fibril formation reduce the Aβ-mediated neurotocixity. In this report, the interaction of amyloid-β (Aβ) with well-described modulators, (-)epigallocatechin-3-g...
Analysis of single algal cells by combining mass spectrometry with Raman and fluorescence mapping.
We implement matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in combination with microspectroscopic mapping. Straightforward coupling of these two detection platforms was possible thanks to the self-aliquoting properties of micro-arrays for mass spectrometry (MAMS). Followin...
A new method to measure oxygen solubility in organic solvents through optical oxygen sensing.
A new method to measure oxygen concentration in air-saturated organic solvents and binary mixtures has been developed. The methodology relies on the ability of HPLC columns to retain the molecular oxygen contained in different types of solvents which are injected into the system at 298.15 K. The out...
A highly specific BODIPY-based probe localized in mitochondria for HClO imaging.
An oxime containing fluorescent probe based on a BODIPY scaffold was successfully designed and used for HClO determination with rapid response, low detection limits and high selectivity. Confocal fluorescence microscopy demonstrated that the probe could permeate the mitochondria and made possible th...
Design of a universal biointerface for sensitive, selective, and multiplex detection of biomarkers using surface plasmon resonance imaging.
This paper reports on the sensitive, selective, and simultaneous detection of four protein biomarkers involved in metastasis of various cancers, namely Fas, angiopoietin-2 (Ang-2), human epidermal growth factor receptor 2 (HER2), and matrix metallopeptidase-9 (MMP-9) using an antibody-conjugated qua...
Alteration of Asian lacquer: in-depth insight using a physico-chemical multiscale approach.
Oriental lacquer has been used in Asian countries for thousands of years as a durable and aesthetic coating material for its adhesive, consolidating, protective and decorative properties. Although these objects are made from an unusual material in Occident, Western museum collections host many lacqu...
Preconcentration determination of arsenic species by sorption of As(V) on Amberlite IRA-410 coupled with fluorescence quenching of L-cystein...
A simple and accurate method for arsenic speciation analysis in natural and drinking water samples is described in which preconcentration of arsenic as As(V) was coupled with spectrofluorometric determination. The extracted As(V) species with a column containing Amberlite IRA-410 were subjected to L...
Development of a high-throughput assay for measuring lipase activity using natural triacylglycerols coated on microtiter plates.
We have designed a convenient, specific, sensitive and continuous lipase assay based on the use of natural triacylglycerols (TAGs) from the Aleurites fordii seed oil which contains α-eleostearic acid (9,11,13,cis,trans,trans-octadecatrienoic acid) and which was coated in the wells of microtiter plat...
Ambient pressure thermal desorption ionization mass spectrometry for the analysis of substances of forensic interest.
The large backlog of forensic samples needing chemical analysis requires new tools with high specificity, throughput, and reliability. Mass spectrometric techniques have the specificity and reliability necessary for identification, but often require sample preparation before analysis. The growth of...