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- Ebscoatoz, Gale, Ebsconet, Sciencedirect from 1960, CSA, Proquest, Ingenta, Ebsco, and Rcgp
Recombinant expression of His-tagged saposin B and pH-dependent binding to the lipid coenzyme Q10
Bacterial transformation using micro-shock waves
Analysis of the CYP21A1P pseudogene: Indication of mutational diversity and CYP21A2-like and duplicated CYP21A2 genes
We found that about 10% of CYP21A1P alleles (n = 195 chromosomes) presented the CYP21A2 sequence; frequencies of P30, V281, Q318, and R356 in that locus were ∼24%, ∼21%, ∼11%, and ∼34%, respectively, and ∼90% of the CYP21A1P alleles had 15 mutated loci. In addition, ∼2.5% (n = 5 chromoso...
Optimizing microarray-based in situ transcription-translation of proteins for matrix-assisted laser desorption ionization mass spectrometry
We describe the fabrication in situ of protein arrays optimized for MALDI characterization. Using the Green Fluorescent Protein (GFP) both as an epitope for immobilization and as a gauge for relative protein expression, we were able to generate amounts of protein on the array slides sufficient for M...
Kinetic characterization of amyloid-beta 1-42 aggregation with a multimethodological approach
Extensive evidence suggests that the self-assembly of amyloid-beta peptide (Aβ) is a nucleation-dependent process, which involves the formation of several oligomeric intermediates. Despite neuronal toxicity has been recently related to Aβ soluble oligomers, results from aggregation...
Advantages of isothermal titration calorimetry for xylanase kinetics in comparison to chemical-reducing-end assays
We employ a subsequent oxidation of hydrolysis products by carbohydrate oxidase and catalase. Here we show that the coupled assay with carbohydrate oxidase and catalase can be used to measure enzyme kinetics of a GH10 xylanase from Aspergillus aculeatus on birch xylan and wheat arabinoxylan. Results...
Mycotoxin detection on antibody-immobilized conducting polymer-supported electrochemically polymerized acacia gum
Electrochemical polymerization of acacia gum (AG) was initiated by electroactive aniline (PANI) monomers by radical cation formation and their coupling reactions with AG molecules. R(CT) value obtained from electrochemical impedance spectroscopy analysis at various AG concentration w...
Antisense PCR: A simple and robust method for performing nested single-tube PCR
We developed a simple and robust closed single-tube nested PCR method (antisense PCR). The method uses antisense oligonucleotides which carry a 5' tag and which can potentially hybridise to the 3' ends of the outer primers, depending on the annealing temperature. During initial cycles, which are per...
Chi-square comparison of tryptic peptide-to-protein distributions of tandem mass spectrometry from blood with those of random expectation
Proteomics uses tandem mass spectrometers and correlation algorithms to match peptides and their fragment spectra to amino acid sequences. The replication of multiple liquid chromatography experiments with electrospray ionization of peptides and tandem mass spectrometry (LC-ESI-MS/MS...
Identification of 1- and 3-methylhistidine as biomarkers of skeletal muscle toxicity by nuclear magnetic resonance-based metabolic profiling
Nuclear magnetic resonance (NMR)-based metabolomic profiling identified urinary 1- and 3-methylhistidine (1- and 3-MH) as potential biomarkers of skeletal muscle toxicity in Sprague-Dawley rats following 7 and 14 daily doses of 0.5 or 1mg/kg cerivastatin. These metabolites were highly correlated to...