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- Gale, Acm, ACS, CSA, Ebscoatoz, Ebsconet, Proquest, and Ebsco
This paper describes the construction of a labeless immunosensor for the antibiotic ciprofloxacin in milk and its interrogation using an ac impedance protocol. Commercial screen-printed carbon electrodes were used as the basis for the sensor. Polyaniline was electrodeposited onto the sensors and...
Analytical analyses of highly complex mixtures, such as biofluids or liquid food products, often give rise to signals for unknown compounds, particularly for compounds at low concentration. Here we compare two conventional chemometric approaches for NMR spectral analysis ("spectral binning" and ...
We present a rapid method for the identification of viruses using microfluidic chip gel electrophoresis (CGE) of high-copy number proteins to generate unique protein profiles. Viral proteins are solubilized by heating at 95 degrees C in borate buffer containing detergent (5 min), then labeled wi...
2-Mercaptobenzothiazole (MBT) is employed for the first time as a matrix for the analysis of lipids from tissue extracts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We demonstrate that the performance of MBT is superior to that of the matrixes commonly emp...
A sensitive electrochemical DNA sensor based on nanoporous gold (NPG) electrode and multifunctional encoded Au nanoparticle (AuNP) was developed. The NPG electrode was prepared with a simple dealloying strategy, by which silver was dissoluted from silver/gold alloys in nitric acid, making the ac...
Three different peptides (His-Glu-Pro-Ser, His-Gly-Ser-Ala and Glu-Pro-Ser-Ala) were selected and tested to be used as affinity binding receptors for organophosphate and carbamate pesticides. The peptides were rationally designed by mimicking acetylcholinesterase active site. The simulated bindi...
Discussed here is the preparation, detailed purification, and evaluation of nitroavidin as a ligand for surface capture and release of biotinylated proteins. Avidin from chicken egg white was nitrated using dilute tetranitromethane solutions. UV-vis spectroscopy was used to show decreased bindin...
The target-decoy database search strategy is often applied to determine the global false-discovery rate (FDR) of peptide identifications in proteome research. However, the confidence of individual peptide identification is typically not determined. In this study, we introduced an approach for th...
Analyzing the intracellular contents and enzymatic activities of single cells is important for studying the physiological and pathological activities at the cellular level. For this purpose, we developed a simple single-cell lysis method by using a dense array of microwells of 10-30-pL volume fa...
A procedure based on microwave-induced combustion coupled to flame furnace (FF) atomic absorption spectrometry (FF-AAS) was used for analysis of solid samples. Botanical samples were prepared as pellets and introduced into a quartz holder device. This device was fitted to a glass chamber that wa...