Analytical chemistry

Print ISSN
0003-2700
Electronic ISSN
1520-6882
Impact factor
5.874
Publisher
ACS
URL
http://pubs.acs.org/journals/ancham/index.html
Usage rank
112
Article count
21100
Free count
443
Free percentage
0.0209953
PDFs via platforms
Gale, Acm, ACS, CSA, Ebscoatoz, Ebsconet, Proquest, and Ebsco

  1. On-line digestion system for protein characterization and proteome analysis.

    Analytical chemistry 80(23):8930 (2008) PMID 19551971 PMCID PMC2755646

    An efficient on-line digestion system that reduces the number of sample manipulation steps has been demonstrated for high-throughput proteomics. By incorporating a pressurized sample loop into a liquid chromatography-based separation system, both sample and enzyme (e.g., trypsin) can be simultan...
  2. Effect of sample heterogeneity on the interpretation of QCM(-D) data: comparison of combined quartz crystal microbalance/atomic force microscopy measurements with finite element method modeling.

    Analytical chemistry 80(23):8891 (2008) PMID 18954085

    A quartz crystal microbalance was integrated into an AFM in order to monitor the adsorption of biomolecules to the resonator surface with both atomic force microscopy and microgravimetry. The comparison between the two techniques allows the fractional coverage of the surface, theta, to be correl...
  3. High-throughput quantitative polymerase chain reaction in picoliter droplets.

    Analytical chemistry 80(23):8975 (2008) PMID 19551929 PMCID PMC2771884

    Limiting dilution PCR has become an increasingly useful technique for the detection and quantification of rare species in a population, but the limit of detection and accuracy of quantification are largely determined by the number of reactions that can be analyzed. Increased throughput may be ac...
  4. Online laser desorption-multiphoton postionization mass spectrometry of individual aerosol particles: molecular source indicators for particles emitted from different traffic-related and wood combustion sources.

    Analytical chemistry 80(23):8991 (2008) PMID 18983175

    Direct inlet aerosol mass spectrometry plays an increasingly important role in applied and fundamental aerosol and nanoparticle research. Laser desorption/ionization (LDI) based techniques for single particle time-of-flight mass spectrometry (LDI-SP-TOFMS) are a promising approach in the chemica...
  5. Profiling and imaging of lipids on brain and liver tissue by matrix-assisted laser desorption/ ionization mass spectrometry using 2-mercaptobenzothiazole as a matrix.

    Analytical chemistry 80(23):9105 (2008) PMID 18959430

    2-Mercaptobenzothiazole (MBT) is employed for the first time as a matrix for the analysis of lipids from tissue extracts using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. We demonstrate that the performance of MBT is superior to that of the matrixes commonly emp...
  6. A new dynamic in mass spectral imaging of single biological cells.

    Analytical chemistry 80(23):9058 (2008) PMID 19551933

    Time-of-flight secondary ion mass spectrometry (TOF-SIMS) has unique capabilities in the area of high-resolution mass spectrometric imaging of biological samples. The technique offers parallel detection of native and non-native molecules at physiological concentrations with potentially submicrom...
  7. Ion mobility spectrometry/mass spectrometry snapshots for assessing the molecular compositions of complex polymeric systems.

    Analytical chemistry 80(23):9073 (2008) PMID 19551934

    The synthesis of increasingly complex polymers has created daunting, sometimes insurmountable problems for their chemical analysis. The importance is magnified by outsourcing of production and their use in consumer products, including medical devices and food storage, and therefore requires a ne...
  8. Multiple isotopic labels for quantitative mass spectrometry.

    Analytical chemistry 80(23):9298 (2008) PMID 19551992 PMCID PMC2771887

    Quantitative mass spectrometry is often performed using isotopically labeled samples. Although the 4-trimethylammoniumbutyryl (TMAB) labels have many advantages over other isotopic tags, only two forms have previously been synthesized (i.e., a heavy form containing nine deuteriums and a light fo...
  9. Nitroavidin as a ligand for the surface capture and release of biotinylated proteins.

    Analytical chemistry 80(23):9336 (2008) PMID 19551994

    Discussed here is the preparation, detailed purification, and evaluation of nitroavidin as a ligand for surface capture and release of biotinylated proteins. Avidin from chicken egg white was nitrated using dilute tetranitromethane solutions. UV-vis spectroscopy was used to show decreased bindin...
  10. Single-cell chemical lysis method for analyses of intracellular molecules using an array of picoliter-scale microwells.

    Analytical chemistry 80(23):9141 (2008) PMID 19551983

    Analyzing the intracellular contents and enzymatic activities of single cells is important for studying the physiological and pathological activities at the cellular level. For this purpose, we developed a simple single-cell lysis method by using a dense array of microwells of 10-30-pL volume fa...
  11. Impedance biosensor for peanut protein Ara h 1.

    Analytical chemistry 80(23):9157 (2008) PMID 19551984

    A reagentless electrochemical impedance biosensor for detection of peanut protein Ara h 1, one of the allergenic proteins found in peanuts, has been demonstrated using an Au substrate onto which an antibody film has been immobilized. Following initial stabilization of the self-assembled monolaye...
  12. Multiplex ligation-dependent probe amplification technique for copy number analysis on small amounts of DNA material.

    Analytical chemistry 80(23):9363 (2008) PMID 19551952

    The multiplex ligation-dependent probe amplification (MLPA) technique is a sensitive technique for relative quantification of up to 50 different nucleic acid sequences in a single reaction, and the technique is routinely used for copy number analysis in various syndromes and diseases. The aim of...
  13. Memory effects in compound-specific D/H analysis by gas chromatography/pyrolysis/isotope-ratio mass spectrometry.

    Analytical chemistry 80(23):9162 (2008) PMID 19551939

    Compound-specific analyses of lipid D/H ratios often encounter ranges of 300 per thousand or more, and experiments using D-enriched water to study fractionations often extend the range up to 1000 per thousand. Here we show that for such large dynamic ranges in D/H ratio, isotopic "memory" betwee...
  14. Effects of binding of metals to the hydrogel and filter membrane on the accuracy of the diffusive gradients in thin films technique.

    Analytical chemistry 80(23):9220 (2008) PMID 19551987

    The effect of metal binding to the diffusion layer of diffusive gradients in thin films (DGT) on measurements of time-integrated concentrations (CDGT) was studied experimentally and using a numerical model. Cationic divalent Cu and Cu-fulvic complexes bind to sites in the filter membrane and pol...
  15. Integration of microdialysis sampling and microchip electrophoresis with electrochemical detection.

    Analytical chemistry 80(23):9257 (2008) PMID 19551945 PMCID PMC2771943

    Here we describe the fabrication, optimization, and application of a microfluidic device that integrates microdialysis (MD) sampling, microchip electrophoresis (ME), and electrochemical detection (EC). The manner in which the chip is produced is reproducible and enables the fixed alignment of th...
  16. Calibration-free quantitative application of in situ Raman spectroscopy to a crystallization process.

    Analytical chemistry 80(23):9240 (2008) PMID 19551944

    In this work, in situ Raman spectroscopy is applied in a quantitative manner to crystal suspensions during crystallization in order to monitor a solvent-mediated polymorph transformation without the use of a calibration model. Assuming a linear dependency of the Raman signal intensity on the sol...
  17. Evaluating low-intensity unknown signals in quantitative proton NMR mixture analysis.

    Analytical chemistry 80(23):8956 (2008) PMID 19551928

    Analytical analyses of highly complex mixtures, such as biofluids or liquid food products, often give rise to signals for unknown compounds, particularly for compounds at low concentration. Here we compare two conventional chemometric approaches for NMR spectral analysis ("spectral binning" and ...
  18. Detection of fluoroquinolone antibiotics in milk via a labeless immunoassay based upon an alternating current impedance protocol.

    Analytical chemistry 80(23):9233 (2008) PMID 19551988

    This paper describes the construction of a labeless immunosensor for the antibiotic ciprofloxacin in milk and its interrogation using an ac impedance protocol. Commercial screen-printed carbon electrodes were used as the basis for the sensor. Polyaniline was electrodeposited onto the sensors and...
  19. Imaging localized astrocyte ATP release with firefly luciferase beads attached to the cell surface.

    Analytical chemistry 80(23):9316 (2008) PMID 19551993

    Extracellular adenosine triphosphate (ATP) functions as a signaling molecule in many cell regulation processes. The traditional firefly luciferase assays measure the ATP release as a signal increase with time using a luminometer. Recently, advanced cell imaging techniques using charge-coupled de...
  20. Nitroavidin as a ligand for the surface capture and release of biotinylated proteins.

    Analytical chemistry 80(23):9336 (2008) PMID 19551994

    Discussed here is the preparation, detailed purification, and evaluation of nitroavidin as a ligand for surface capture and release of biotinylated proteins. Avidin from chicken egg white was nitrated using dilute tetranitromethane solutions. UV-vis spectroscopy was used to show decreased bindin...