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Analytical chemistry

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  1. Measurement of ZnO nanoparticles using diffusive gradients in thin films: binding and diffusional characteristics.

    Analytical chemistry 86(12):5906 (2014) PMID 24831848

    Rapid growth in finding new applications for manufactured nanomaterials (MNM) has recently been accompanied by awareness about their related adverse toxicological and environmental impacts. Due to their intrinsic nature, measuring available concentrations of MNMs in the environment is a major challe...
  2. Evaluation of fast 2D NMR for metabolomics.

    Analytical chemistry 86(12):5946 (2014) PMID 24856256

    We evaluated two methods for the acceleration of nD NMR, ultrafast (UF) and nonuniform sampling (NUS), in the context of metabolomics. To this end, model samples mimicking the metabolic profile variations in serum from subjects affected by colorectal cancer and controls were analyzed by 1D (1)H NMR...
  3. Red emissive two-photon probe for real-time imaging of mitochondria trafficking.

    Analytical chemistry 86(12):5638 (2014) PMID 24893806

    We report here a red emissive two-photon probe for mitochondria (CMT-red) that showed high selectivity and robust staining ability for mitochondria, high photostability under a two-photon microscopy imaging condition, and low cytotoxicity. This probe can be easily loaded into live cells and tissue a...
  4. Development of an engineered bioluminescent reporter phage for the sensitive detection of viable Salmonella typhimurium.

    Analytical chemistry 86(12):5858 (2014) PMID 24806327

    We developed an advanced bioluminescent reporter phage SPC32H-CDABE by inserting a bacterial luxCDABE operon into the Salmonella temperate phage SPC32H genome. Whole SPC32H genome sequencing enabled the selection of nonessential genes, which can be replaced with approximately 6-kb luxCDABE operon, w...
  5. Membrane-assisted isoelectric focusing device as a micropreparative fractionator for two-dimensional shotgun proteomics.

    Analytical chemistry 86(12):5728 (2014) PMID 24824042

    We introduced an online multijunction capillary isoelectric focusing (OMJ-CIEF) fractionator to fractionate proteins and peptides in electrospray-friendly solution. In this follow-up study, the original configuration of the fractionator was modified to improve the resolving power and reproducibility...
  6. Sensitive method for quantification of octamethylcyclotetrasiloxane (D4) and decamethylcyclopentasiloxane (D5) in end-exhaled air by thermal...

    Analytical chemistry 86(12):5794 (2014) PMID 24833048

    Octamethylcyclotetrasiloxane (D4) and decamethylpentasiloxane (D5) are used as ingredients for personal care products (PCPs). Because of the use of these PCPs, consumers are exposed daily to D4 and D5. A sensitive analytical method was developed for analysis of D4 and D5 in end-exhaled air by therma...
  7. Hydrogen sulfide deactivates common nitrobenzofurazan-based fluorescent thiol labeling reagents.

    Analytical chemistry 86(12):6032 (2014) PMID 24852143 PMCID PMC4063329

    We report the differential responses of common electrophilic fluorescent thiol labeling reagents, including nitrobenzofurazan-based scaffolds, maleimides, alkylating agents, and electrophilic aldehydes, toward cysteine and H2S. Although H2S reacted with all of the investigated scaffolds, the photoph...
  8. Dual-peak electrogenerated chemiluminescence of carbon dots for iron ions detection.

    Analytical chemistry 86(12):5620 (2014) PMID 24855934

    We are here to report a dual-peak ECL system of CDs, one at -2.84 V (ECL-1) and the other at -1.71 V (ECL-2) during the cyclic sweep between -3.0 and 3.0 V at scan rate of 0.2 V s(-1) in 0.1 M tetrabutyl ammonium bromide (TBAB) ethanol solution, which is more efficiency to distinguish metallic ions...
  9. Quantification of HIV-1 DNA using real-time recombinase polymerase amplification.

    Analytical chemistry 86(12):5615 (2014) PMID 24873435

    We describe a real-time RPA assay with an internal positive control and an algorithm that analyzes real-time fluorescence data to quantify HIV-1 DNA. We show that DNA concentration and the onset of detectable amplification are correlated by an exponential standard curve. In a set of experiments in w...
  10. Differential detection of tumor cells using a combination of cell rolling, multivalent binding, and multiple antibodies.

    Analytical chemistry 86(12):6088 (2014) PMID 24892731 PMCID PMC4066911

    We developed a novel platform that captures tumor cells at significantly improved efficiency in vitro using a unique biomimetic combination of two physiological processes: E-selectin-induced cell rolling and poly(amidoamine) (PAMAM) dendrimer-mediated strong multivalent binding. Herein, we have engi...