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- Gale, Acm, ACS, CSA, Ebscoatoz, Ebsconet, Proquest, and Ebsco
Ratiometric fluorescent probe based on gold nanoclusters and alizarin red-boronic Acid for monitoring glucose in brain microdialysate.
Glucose monitoring with high sensitivity and accuracy in the cerebrospinal fluid is a challenge for evaluating the role of glucose in the physiological and pathological processes. In this work, a ratiometric fluorescent probe for sensing glucose was developed. In the probe, the gold nanoclusters pro...
Method of Atmospheric Pressure Charge Stripping for Electrospray Ionization Mass Spectrometry and Its Application for the Analysis of Large ...
We introduce a new atmospheric pressure charge stripping (AP-CS) method for the electrospray ionization mass spectrometry (ESI-MS) analysis of heterogeneous mixtures, utilizing ion/ion proton transfer reactions within an experimental ion source to remove excess charge from sample ions and thereby re...
Semiautomated pH Gradient Ion-Exchange Chromatography of Monoclonal Antibody Charge Variants.
A new approach using a chromatography system equipped with isocratic pumps and an electrolytic eluent generator (EG) is introduced, replacing external pH gradient delivery using conventional gradient systems, in which bottled buffers with preadjusted pH are mixed using a gradient pump. The EG is cap...
Quantifying biomass composition by gas chromatography/mass spectrometry.
We developed a set of methods for the quantification of four major components of microbial biomass using gas chromatography/mass spectrometry (GC/MS). Specifically, methods are described to quantify amino acids, RNA, fatty acids, and glycogen, which comprise an estimated 88% of the dry weight of Esc...
Automatic generic registration of mass spectrometry imaging data to histology using nonlinear stochastic embedding.
We present a fully automated generic approach for registering mass spectrometry imaging data to histology and demonstrate its capabilities for multiple mass analyzers, multiple ionization sources, and multiple tissue types....
Two-dimensional photonic crystal sensors for visual detection of lectin concanavalin a.
We fabricated a two-dimensional (2-D) photonic crystal lectin sensing material that utilizes light diffraction from a 2-D colloidal array attached to the surface of a hydrogel that contains mannose carbohydrate groups. Lectin-carbohydrate interactions create hydrogel cross-links that shrink the hydr...
Absolute Quantification of Histone PTM Marks by MRM-Based LC-MS/MS.
We recently developed a multiple reaction monitoring (MRM) based LC-MS/MS method to analyze 42 targeted histone peptides. By using stable isotopic labeled peptides as internal standards that are spiked into the reconstituted solutions, this method allows to measure absolute concentration of the tryp...
Multiplexed analysis of protein-ligand interactions by fluorescence anisotropy in a microfluidic platform.
We implemented a homogeneous fluorescence anisotropy-based binding assay in an automated microfluidic chip to simultaneously interrogate >2300 pairwise interactions. We demonstrated the utility of this platform in determining the binding affinities between chromatin-regulatory proteins and different...
Photoelectrochemical aptasensing of kanamycin using visible light-activated carbon nitride and graphene oxide nanocomposites.
We developed a novel PEC aptamer sensor for the specific detection of kanamycin using water-dispersible graphite-like carbon nitride (w-g-C3N4) as visible light-active material and aptamer as the biorecognition element. While a suitable amount of graphene oxide (GO) was doped in w-g-C3N4, the visibl...
Automated lipid membrane formation using a polydimethylsiloxane film for ion channel measurements.
We used a PDMS thin-film, a porous-structured hydrophobic polymer, and squalene, a high viscosity solvent, to facilitate membrane formation, whereas the absorption rates of solvents were controlled to achieve consistent BLM formation time. We successfully reduced thinning-out time down to <1 h as we...