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Analytical chemistry

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  1. Investigation of the Persistence of Nerve Agent Degradation Analytes on Surfaces through Wipe Sampling and Detection with Ultrahigh Performa...

    Analytical chemistry 87(2):1034 (2015) PMID 25495198

    The persistence of chemical warfare nerve agent degradation analytes on surfaces is important, from indicating the presence of nerve agent on a surface to guiding environmental restoration of a site after a release. Persistence was investigated for several chemical warfare nerve agent degradation an...
  2. Emulsion PCR Significantly Improves Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures-Based Aptamer Selection: Allowing for E...

    Analytical chemistry 87(2):1411 (2015) PMID 25495441

    We proposed the use of emulsion PCR (ePCR), which is known to reduce byproduct formation, as a PCR mode for coupling with NECEEM partitioning. For the first time, we tested the advantages of ePCR in NECEEM-based aptamer selection to a medically relevant DNA repair enzyme, ABH2. We report that the co...
  3. LED-Based UV Absorption Detector with Low Detection Limits for Capillary Liquid Chromatography.

    Analytical chemistry 87(2):1381 (2015) PMID 25496031

    A 260 nm deep UV LED-based absorption detector with low detection limits was developed and integrated with a small nanoflow pumping system. The detector is small in size (5.2 × 3.0 cm) and weighs only 85 g (without electronics). This detector was specifically designed and optimized for on-column det...
  4. Phosphate ion targeted colorimetric and fluorescent probe and its use to monitor endogeneous phosphate ion in a hemichannel-closed cell.

    Analytical chemistry 87(2):1196 (2015) PMID 25511872

    Fluorescent probe 1, the first inorganic phosphate (Pi) targeted colorimetric and fluorescent probe to detect endogenous Pi in hemichannel-closed cells, has been developed. Probe 1 undergoes a unique Pi induced hydrolytic reaction in DMSO-HEPES (V/V = 9:1) buffered (0.02 M, pH 7.4) solutions that pr...
  5. Practical assay for nitrite and nitrosothiol as an alternative to the griess assay or the 2,3-diaminonaphthalene assay.

    Analytical chemistry 87(2):1274 (2015) PMID 25519711

    We designed a novel probe (NT555) for nitrite detection, which displays superior detection kinetics and sensitivity. NT555 was constructed following our "covalent-assembly" probe design principle. Upon detection, it affords a gigantic bathochromic shift of the absorption spectrum and a sensitive tur...
  6. Simple Quantitative Determination of Potent Thiols at Ultratrace Levels in Wine by Derivatization and High-Performance Liquid Chromatography...

    Analytical chemistry 87(2):1226 (2015) PMID 25562625

    Volatile sulfur compounds contribute characteristic aromas to foods and beverages and are widely studied, because of their impact on sensory properties. Certain thiols are particularly important to the aromas of roasted coffee, cooked meat, passion fruit, grapefruit, and guava. These same thiols enh...
  7. NMR spectroscopy for metabolomics and metabolic profiling.

    Analytical chemistry 87(1):133 (2015) PMID 25375201

  8. Fluorometric method for inorganic pyrophosphatase activity detection and inhibitor screening based on click chemistry.

    Analytical chemistry 87(1):816 (2015) PMID 25483562

    A fluorometric method for pyrophosphatase (PPase) activity detection was developed based on click chemistry. Cu(II) can coordinate with pyrophosphate (PPi), the addition of pyrophosphatase (PPase) into the above system can destroy the coordinate compound because PPase catalyzes the hydrolysis of PPi...
  9. Tattoo-Based Noninvasive Glucose Monitoring: A Proof-of-Concept Study.

    Analytical chemistry 87(1):394 (2015) PMID 25496376

    We present a proof-of-concept demonstration of an all-printed temporary tattoo-based glucose sensor for noninvasive glycemic monitoring. The sensor represents the first example of an easy-to-wear flexible tattoo-based epidermal diagnostic device combining reverse iontophoretic extraction of intersti...
  10. Accumulation and separation of membrane-bound proteins using hydrodynamic forces.

    Analytical chemistry 83(2):604 (2011) PMID 21155531

    We demonstrate how hydrodynamic forces can be used to both accumulate and separate membrane-bound proteins in their native state. A supported lipid bilayer (SLB) was formed inside a microfluidic channel with the two proteins streptavidin (SA) and cholera toxin (CT) coupled to receptors in the lipid...