Biochimica et Biophysica Acta

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  1. The importance of the benzoic acid carboxylate moiety for substrate recognition by CYP199A4 from Rhodopseudomonas palustris HaA2.

    Biochimica et Biophysica Acta 1864(6):667 (2016) PMID 26969786

    The cytochrome P450 enzyme CYP199A4 can efficiently demethylate 4-methoxybenzoic acid. The substrate is positioned in the enzyme active site with the methoxy group ideally positioned for demethylation. This occurs through interactions of hydrophobic benzene ring with aromatic phenylalanine resid...
  2. Dual mechanism of action of the atypical tetracycline chelocardin.

    Biochimica et Biophysica Acta 1864(6):645 (2016) PMID 26969785

    Classical tetracyclines targeting the protein biosynthesis machinery are commonly applied in human and veterinary medicine. The development and spread of resistance seriously compromise the successful treatment of bacterial infections. The atypical tetracycline chelocardin holds promise as it re...
  3. Substitutions mimicking deimination and phosphorylation of 18.5-kDa myelin basic protein exert local structural effects that subtly influence its global folding.

    Biochimica et Biophysica Acta 1858(6):1262 (2016) PMID 26903219

    Intrinsically-disordered proteins (IDPs) present a complex interplay of conformational variability and multifunctionality, modulated by environment and post-translational modifications. The 18.5-kDa myelin basic protein (MBP) is essential to the formation of the myelin sheath of the central nerv...
  4. Alanine scan and (2)H NMR analysis of the membrane-active peptide BP100 point to a distinct carpet mechanism of action.

    Biochimica et Biophysica Acta 1858(6):1328 (2016) PMID 26975251

    The short membrane-active peptide BP100 [KKLFKKILKYL-NH2] is known as an effective antimicrobial and cell penetrating agent. For a functional alanine scan each of the 11 amino acids was replaced with deuterated Ala-d3, one at a time. MIC assays showed that a substitution of Lys did not affect th...
  5. Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments.

    Biochimica et Biophysica Acta 1858(6):1306 (2016) PMID 26922884

    G protein-coupled receptors are of key significance for biomedical research. Streamlined approaches for their efficient recombinant production are of pivotal interest in order to explore their intrinsic conformational dynamics and complex ligand binding behavior. We have systematically optimized...
  6. Both idebenone and idebenol are localized near the lipid-water interface of the membrane and increase its fluidity.

    Biochimica et Biophysica Acta 1858(6):1071 (2016) PMID 26926421

    Idebenone is a synthetic analog of coenzyme Q; both share a quinone moiety but idebenone has a shorter lipophilic tail ending with a hydroxyl group. Differential scanning calorimetry experiments showed that both idebenone and idebenol widened and shifted the phase transition of 1,2-dipalmitoylph...
  7. The Alzheimer's disease Aβ peptide binds to the anionic DMPS lipid bilayer.

    Biochimica et Biophysica Acta 1858(6):1118 (2016) PMID 26947182

    We have applied isobaric-isothermal replica exchange molecular dynamics (REMD) and the all-atom explicit water model to study binding of Aβ10-40 peptide to the anionic DMPS bilayer. To provide comparison with a zwitterionic bilayer, we used our previous REMD simulations probing binding of the sa...
  8. Alternative packing of EGFR transmembrane domain suggests that protein-lipid interactions underlie signal conduction across membrane.

    Biochimica et Biophysica Acta 1858(6):1254 (2016) PMID 26903218

    The human epidermal growth factor receptor (EGFR) of HER/ErbB receptor tyrosine kinase family mediates a broad spectrum of cellular responses transducing biochemical signals via lateral dimerization in plasma membrane, while inactive receptors can exist in both monomeric and dimeric forms. Recen...
  9. Membrane interactions of proline-rich antimicrobial peptide, Chex1-Arg20, multimers.

    Biochimica et Biophysica Acta 1858(6):1236 (2016) PMID 26926423

    The increasing prevalence of antibiotic-resistant pathogens requires the development of new antibiotics. Proline-rich antimicrobial peptides (PrAMPs), including native apidaecins, Bac7, and oncocins or designed A3APO, show multi-modal actions against pathogens together with immunostimulatory act...
  10. Nanoscale characterization of vesicle adhesion by normalized total internal reflection fluorescence microscopy.

    Biochimica et Biophysica Acta 1858(6):1244 (2016) PMID 26972045

    We recently proposed a straightforward fluorescence microscopy technique to study adhesion of Giant Unilamellar Vesicles. This technique is based on dual observations which combine epi-fluorescence microscopy and total internal reflection fluorescence (TIRF) microscopy: TIRF images are normalize...
  11. Spatial distribution and activity of Na(+)/K(+)-ATPase in lipid bilayer membranes with phase boundaries.

    Biochimica et Biophysica Acta 1858(6):1390 (2016) PMID 26994932

    We have reconstituted functional Na(+)/K(+)-ATPase (NKA) into giant unilamellar vesicles (GUVs) of well-defined binary and ternary lipid composition including cholesterol. The activity of the membrane system can be turned on and off by ATP. The hydrolytic activity of NKA is found to depend on me...
  12. Oxidative stress and glyoxalase I activity mediate dicarbonyl toxicity in MCF-7 mamma carcinoma cells and a tamoxifen resistant derivative.

    Biochimica et Biophysica Acta 1860(6):1272 (2016) PMID 26971627

    Acquired tamoxifen resistance is a significant problem in estrogen receptor positive breast cancer. In a cellular model, tamoxifen resistance was associated with increased sensitivity towards toxic dicarbonyls and reduced free sulfhydryl group content. We here analyzed the role of oxidative stre...
  13. Roles of acetyl-CoA synthetase (ADP-forming) and acetate kinase (PPi-forming) in ATP and PPi supply in Entamoeba histolytica.

    Biochimica et Biophysica Acta 1860(6):1163 (2016) PMID 26922831

    Acetate is an end-product of the PPi-dependent fermentative glycolysis in Entamoeba histolytica; it is synthesized from acetyl-CoA by ADP-forming acetyl-CoA synthetase (ACS) with net ATP synthesis or from acetyl-phosphate by a unique PPi-forming acetate kinase (AcK). The relevance of these enzym...
  14. Zebrafish Tg(hb9:MTS-Kaede): a new in vivo tool for studying the axonal movement of mitochondria.

    Biochimica et Biophysica Acta 1860(6):1247 (2016) PMID 26968460

    Deregulation of axonal transport in neurons is emerging as the major cause of many neurodegenerative diseases in human, such as Charcot-Marie-Tooth (CMT) neuropathy. However, little is known about how mitochondria move in vivo and whether cell culture systems truly represent what happens in livi...
  15. 'Lollipop'-shaped helical structure of a hybrid antimicrobial peptide of temporin B-lipopolysaccharide binding motif and mapping cationic residues in antibacterial activity.

    Biochimica et Biophysica Acta 1860(6):1362 (2016) PMID 27015761

    Temporins are attractive templates for the development of antibiotics. However, many temporins are inactive against Gram-negative bacteria. Previously, we demonstrated conjugation of a lipopolysaccharide binding motif peptide to temporins yielded hybrid non-haemolytic AMPs that killed several Gr...
  16. Insights into conformational regulation of PfMATE transporter from Pyrococcus furiosus induced by alternating protonation state of Asp41 residue: A molecular dynamics simulation study.

    Biochimica et Biophysica Acta 1860(6):1173 (2016) PMID 26879959

    Multidrug and toxic compound extrusion (MATE) family transporters induce multiple-drug resistance (MDR) of bacterial pathogens and cancer cells, thus causing critical reductions in the therapeutic efficacies of antibiotics and anti-cancer drugs. Unfortunately, to date, the details and intrinsic ...
  17. Whi2 enhances methylmercury toxicity in yeast via inhibition of Akr1 palmitoyltransferase activity.

    Biochimica et Biophysica Acta 1860(6):1326 (2016) PMID 27015763

    We have previously reported that Whi2 enhances the toxicity of methylmercury in yeast. In the present study we examined the proteins known to interact with Whi2 to find those that influence the toxicity of methylmercury. Gene disruption and site-directed mutagenesis were employed to examine the ...
  18. Characterisation of two self-sufficient CYP102 family monooxygenases from Ktedonobacter racemifer DSM44963 which have new fatty acid alcohol product profiles.

    Biochimica et Biophysica Acta 1860(6):1149 (2016) PMID 26825771

    Two self-sufficient CYP102 family encoding genes (Krac_0936 and Krac_9955) from the bacterium Ktedonobacter racemifer DSM44963, which possesses one of the largest bacterial genomes, have been identified. Phylogenetic analysis of both the encoded cytochrome P450 enzymes, Krac0936 and Krac9955. Bo...
  19. Design and biological testing of peptidic dimerization inhibitors of human Hsp90 that target the C-terminal domain.

    Biochimica et Biophysica Acta 1860(6):1043 (2016) PMID 26774645

    Small molecules targeting the dimerization interface of the C-terminal domain of Hsp90, a validated target for cancer treatment, have yet to be identified. Three peptides were designed with the aim to inhibit the dimerization of Hsp90. Computational and biophysical methods examined the α-helical...
  20. Overexpression of angiotensin II type 1 receptor in breast cancer cells induces epithelial-mesenchymal transition and promotes tumor growth and angiogenesis.

    Biochimica et Biophysica Acta 1863(6 Pt A):1071 (2016) PMID 26975580

    The angiotensin II type I receptor (AGTR1) has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression. We sought to investigate the role of AGTR1 in cell proliferation, epithelial-mesenchymal transition (EMT...