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[3-Dimensional microscopy as a method for volume measurement in cells undergoing apoptosis].
We employed recently developed three dimensional microscopy for the measurement of the volume of attached vascular smooth muscle cells transfected with E1A-adenoviral protein. These cells undergo rapid apoptosis in the absence of growth factors or in the presence of staurosporine. In 30-60 min of se...
[Paramagnetic calcium melanins].
We assume the existence of two types of paramagnetic centers in melanin-like polymer: M(2+)-stabilized o-semiquinone radical or bi-radical complex containing o-semiquinone and superoxide anion radicals, stabilized by M2+....
[Singlet-triplet excitation fission in light-harvesting complexes of photosynthetic bacteria and in isolated carotenoids].
Time-resolved electron paramagnetic resonance was used to study the properties of carotenoid triplet states populated in LH2 light-harvesting complexes of phototrophic bacteria Allochromatium minutissimum, Rhodopseudomonas palustris, and in carotenoid films free of bacteriochlorophyll. The study was...
[Reactivation of oxygen accumulation function after complete removal of manganese from the photosystem II particles].
Reactivation of 0² evolution function has been studied in PS-2 particles after complete removal of Mn and water soluble 10, 17, 24, 33 kDa proteins, It has been shown that 0² evolution function in such particles can be reactivated by adding 5 μM Mn² and 20 mM Ca²(+). Preliminary illumination of...
[Suppression of vascular endothelium hyperpermeability by cell-permeating peptide inhibitors of myosin light chain kinase].
Our results validate PIK2 and PIK4 as the leading molecules for the development of novel drugs intended to counteract pathological hyperpermeability of vascular endothelium....
[Blood plasma protein adsorption capacity of perfluorocarbon emulsion stabilized by proxanol 268 (in vitro and in vivo studies)].
The adsorption abilities of the perfluorocarbon emulsion stabilized by Proxanol 268 were investigated in vitro and in vivo. In vitro, the saturation point for the blood plasma proteins was nearly reached after five minutes of incubation of the emulsion with human/rabbit blood plasma...
[Computer modeling of ventricular fibrillation].
Electrical activity of a heart in ventricular fibrillation was modeled as a sum of independent pulse streams with various amplitude-frequency and phase characteristics. Results of computer experiments were compared with those of real physiological experiments on rabbits. Identificati...
[Induction-resonance energy transfer between the terbium-binding peptide and the red fluorescent proteins Dsred2 and TagRFP].
Two novel FRET-pairs: Tb3+ -binding peptide-DsRed2 and Tb3+ -binding peptide-TagRFP have been produced based on the terbium-binding peptide and the red fluorescent proteins DsRed2 and TagRFP. Two induction-resonance energy transfer processes in both FRET-pairs have been registered, from tryptophan o...
[Computer simulation of hydraulic flows in a human eye].
A two-dimensional computer model was developed to describe hydraulic flows inside the human eye. The flow field was described by coupled Navier-Stokes and Darcy equations. The velocity and pressure profiles in the chambers, the wall, and the vitreous body of the normal eye were obtai...
[Mechanical characteristics of synthetic polyelectrolyte gel as a physical model of the cytoskeleton].
A physical model of the cytoskeleton based on synthetic polyelectrolyte hydrogel of polymethacrylic acid has been proposed. From the physicochemical point of view, the structures of polyelectrolyte gel and the cytoskeleton show a high degree of similarity. It was shown that polyelect...