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Biochemical characterization of a new β-1,3-galactosyltransferase WbuP from Escherichia coli O114 that catalyzes the second step in O-antige...
In this study, synthetic acceptor substrate GlcNAc alpha-PO3-PO3-(CH2)11-O-phenyl (GlcNAc-PP-PhU) was employed in glycosyl transferase assays to characterize the WbuP galactosyltransferase activity. This activity was time- and enzyme concentration-dependent. The optimal enzyme activity was observed...
Sialyltransferase inhibitors: consideration of molecular shape and charge/hydrophobic interactions.
We synthesized three types of molecules and tested them against a sialyltransferase. The first type of compounds were designed as substrate mimics in which the phosphate in CMP-Neu5NAc was replaced by a non-hydrolysable, uncharged 1,2,3-triazole moiety. The second type of compound contained a 2-deox...
Enzymatic polymerization to an alternating N-l-cysteinyl chitin derivative: a novel class of multivalent glycopeptidomimetics.
A novel chitin derivative with an alternating N-l-cysteinyl group was successfully prepared. The precursor chitin derivative with an alternating N-l-thioprolinyl group could be obtained via chitinase-catalyzed polymerization of the N'-l-thioprolinyl chitobiose oxazoline derivative as a transition st...
High diastereoselective vinylogous Mannich reaction induced by O-pivaloylated d-galactosylamine as the chiral auxiliary: stereoselective syn...
The diastereospecific formation of β-N-glycosidically linked α,β-unsaturated δ-amino aldehyde derivatives has been achieved with high yield via a vinylogous Mannich reaction. The reaction was performed by using a O-pivaloylated galactosyl amine as a chiral template and AlCl3 as a promoter in THF. (S...
Theoretical study on the mechanism of the side reaction of 1-butyl-3-methylimidazolium cation with d-glucose.
To understand the non-inert nature of ionic liquids in cellulose chemistry, density functional theory calculations were carried out to investigate the reaction of 1-butyl-3-methylimidazolium cation ([bmim](+)) with model compounds α- and β-glucoses (the basic units of cellulose and its degradation p...
The capsular polysaccharide and lipopolysaccharide structures of two carbapenem resistant Klebsiella pneumoniae outbreak isolates.
Carbapenem resistant Klebsiella pneumoniae (CRKP) are isolated with increasing frequency, especially from immunocompromized patients. The capsular polysaccharide (CPS) types of CPKP were not determined. Investigation of two CRKP isolates from a 2011 outbreak at the Clinical Center, the National Inst...
A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties.
A heteroglycan (PS) isolated from the mycelia of Pleurotus ostreatus was found to consist of l-fucose, d-mannose, and d-glucose in a molar ratio of nearly 1:2:3. On the basis of acid hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, H...
Structural elucidation of a biologically active heteroglycan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes.
A water soluble heteroglycan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes consists of d-glucose, d-mannose, and d-galactose residues in a molar ratio of nearly 1:1:1. This polysaccharide showed macrophage, splenocyte,...
Synthesis, characterization, and in vitro evaluation of palmitoylated arabinogalactan with potential for liver targeting.
Arabinogalactan (AG), a water soluble polysaccharide with more than 80 mol% galactose units, was hydrophobized by covalent attachment of palmitoyl chains using a base-catalyzed esterification reaction with the objective of effective amalgamation of arabinogalactan in liposomes for targeting asialogl...
Comparison of N-linked glycosylation of protein C in newborns and adults.
We decided to determine the relative constitution of glycoforms in these age groups. Subtypes of PC in newborn and adult plasmas were distinguished by SDS polyacrylamide electrophoresis and Western blotting, followed by immunological analysis. Newborns were found to have alpha-PC/beta-PC mole ratios...