Journal of Biological Chemistry

Print ISSN
0021-9258
Electronic ISSN
1083-351X
Impact factor
5.328
Publisher
highwire
URL
http://www.jbc.org
Usage rank
3
Article count
163289
Free count
147136
Free percentage
0.901077
PDFs via platforms
Aacr, CSA, American Society for Biochemistry and Molecular Biology, Ama, Nephrology, Endocrine, Aaas, Rupress, Highwire, Csh, and Proquest

  1. Molecular dissection of Erv26p identifies separable cargo binding and coat protein sorting activities.

    Journal of Biological Chemistry 284(36):24049 (2009) PMID 19574229 PMCID PMC2781999

    Efficient export of secretory alkaline phosphatase (ALP) from the endoplasmic reticulum depends on the conserved transmembrane sorting adaptor Erv26p/Svp26p. In the present study we investigated the mechanism by which Erv26p couples pro-ALP to the coat protein complex II (COPII) export machinery...
  2. The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast.

    Journal of Biological Chemistry 284(36):23989 (2009) PMID 19584054 PMCID PMC2781993

    Fission yeast Atf1 is a member of the ATF/CREB basic leucine zipper (bZIP) family of transcription factors with strong homology to mammalian ATF2. Atf1 regulates transcription in response to stress stimuli and also plays a role in controlling heterochromatin formation and recombination. However,...
  3. The role of nitric-oxide synthase in the regulation of UVB light-induced phosphorylation of the alpha subunit of eukaryotic initiation factor 2.

    Journal of Biological Chemistry 284(36):24281 (2009) PMID 19586904 PMCID PMC2782021

    UV light induces phosphorylation of the alpha subunit of the eukaryotic initiation factor 2 (eIF2alpha) and inhibits global protein synthesis. Both eIF2 kinases, protein kinase-like endoplasmic reticulum kinase (PERK) and general control of nonderepressible protein kinase 2 (GCN2), have been sho...
  4. Regulation of Rad51 recombinase presynaptic filament assembly via interactions with the Rad52 mediator and the Srs2 anti-recombinase.

    Journal of Biological Chemistry 284(36):24363 (2009) PMID 19605344 PMCID PMC2782029

    Homologous recombination represents an important means for the error-free elimination of DNA double-strand breaks and other deleterious DNA lesions from chromosomes. The Rad51 recombinase, a member of the RAD52 group of recombination proteins, catalyzes the homologous recombination reaction in t...
  5. B cell antigen receptor endocytosis and antigen presentation to T cells require Vav and dynamin.

    Journal of Biological Chemistry 284(36):24088 (2009) PMID 19586920 PMCID PMC2782002

    Antigen binding to the B cell antigen receptor (BCR) initiates an array of signaling events. These include endocytosis of ligand-receptor complexes via clathrin-coated pits, trafficking of the internalized ligand to lysosomes, degradation of the associated proteins to peptides, and peptide prese...
  6. Antigen-specific proteolysis by hybrid antibodies containing promiscuous proteolytic light chains paired with an antigen-binding heavy chain.

    Journal of Biological Chemistry 284(36):24622 (2009) PMID 19542217 PMCID PMC2782051

    The antigen recognition site of antibodies consists of the heavy and light chain variable domains (V(L) and V(H) domains). V(L) domains catalyze peptide bond hydrolysis independent of V(H) domains (Mei, S., Mody, B., Eklund, S. H., and Paul, S. (1991) J. Biol. Chem. 266, 15571-15574). V(H) domai...
  7. Evolutionary basis of converting a bacterial tRNA synthetase into a yeast cytoplasmic or mitochondrial enzyme.

    Journal of Biological Chemistry 284(36):23954 (2009) PMID 19574213 PMCID PMC2781989

    Previous studies showed that cytoplasmic and mitochondrial forms of yeast valyl-tRNA synthetase (ValRS) are specified by the VAS1 gene through alternative initiation of translation. Sequence comparison suggests that the yeast cytoplasmic (or mature mitochondrial) ValRS contains an N-terminal app...
  8. Generation of digital responses in stress sensors.

    Journal of Biological Chemistry 284(36):23902 (2009) PMID 19570986 PMCID PMC2781984

    Ultrasensitivity, hysteresis (a form of biochemical memory), and all-or-none (digital) responses are important signaling properties for the control of irreversible processes and are well characterized in the c-Jun N-terminal kinase (JNK) system using Xenopus oocytes. Our aim was to study these p...
  9. Inhibition of lysine acetyltransferase KAT3B/p300 activity by a naturally occurring hydroxynaphthoquinone, plumbagin.

    Journal of Biological Chemistry 284(36):24453 (2009) PMID 19570987 PMCID PMC2782038

    Lysine acetyltransferases (KATs), p300 (KAT3B), and its close homologue CREB-binding protein (KAT3A) are probably the most widely studied KATs with well documented roles in various cellular processes. Hence, the dysfunction of p300 may result in the dysregulation of gene expression leading to th...
  10. Dissecting activation of the PAK1 kinase at protrusions in living cells.

    Journal of Biological Chemistry 284(36):24133 (2009) PMID 19574218 PMCID PMC2782007

    The p21-activated kinase (PAK) 1 kinase, an effector of the Cdc42 and Rac1 GTPases, regulates cell protrusions and motility by controlling actin and adhesion dynamics. Its deregulation has been linked to human cancer. We show here that activation of PAK1 is necessary for protrusive activity duri...
  11. Getting along with a little help from my friends.

    Journal of Biological Chemistry 284(36):23885 (2009) PMID 19515841 PMCID PMC2781982

  12. Skeletal muscle AMP-activated protein kinase is essential for the metabolic response to exercise in vivo.

    Journal of Biological Chemistry 284(36):23925 (2009) PMID 19525228 PMCID PMC2781986

    AMP-activated protein kinase (AMPK) has been postulated as a super-metabolic regulator, thought to exert numerous effects on skeletal muscle function, metabolism, and enzymatic signaling. Despite these assertions, little is known regarding the direct role(s) of AMPK in vivo, and results obtained...
  13. Regulation by afadin of cyclical activation and inactivation of Rap1, Rac1, and RhoA small G proteins at leading edges of moving NIH3T3 cells.

    Journal of Biological Chemistry 284(36):24595 (2009) PMID 19589776 PMCID PMC2782049

    Cyclical activation and inactivation of Rho family small G proteins, such as Rho, Rac, and Cdc42, are needed for moving cells to form leading edge structures in response to chemoattractants. However, the mechanisms underlying the dynamic regulation of their activities are not fully understood. W...
  14. Phosphorylated intrinsically disordered region of FACT masks its nucleosomal DNA binding elements.

    Journal of Biological Chemistry 284(36):24610 (2009) PMID 19605348 PMCID PMC2782050

    FACT is a heterodimer of SPT16 and SSRP1, which each contain several conserved regions in the primary structure. The interaction of FACT with nucleosomes induces chromatin remodeling through the combinatorial action of its distinct functional protein regions. However, there is little mechanistic...
  15. The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast.

    Journal of Biological Chemistry 284(36):23989 (2009) PMID 19584054 PMCID PMC2781993

    Fission yeast Atf1 is a member of the ATF/CREB basic leucine zipper (bZIP) family of transcription factors with strong homology to mammalian ATF2. Atf1 regulates transcription in response to stress stimuli and also plays a role in controlling heterochromatin formation and recombination. However,...
  16. Physical interactions between Mcm10, DNA, and DNA polymerase alpha.

    Journal of Biological Chemistry 284(36):24662 (2009) PMID 19608746 PMCID PMC2782055

    Mcm10 is an essential eukaryotic protein required for the initiation and elongation phases of chromosomal replication. Specifically, Mcm10 is required for the association of several replication proteins, including DNA polymerase alpha (pol alpha), with chromatin. We showed previously that the in...
  17. Molecular dissection of Erv26p identifies separable cargo binding and coat protein sorting activities.

    Journal of Biological Chemistry 284(36):24049 (2009) PMID 19574229 PMCID PMC2781999

    Efficient export of secretory alkaline phosphatase (ALP) from the endoplasmic reticulum depends on the conserved transmembrane sorting adaptor Erv26p/Svp26p. In the present study we investigated the mechanism by which Erv26p couples pro-ALP to the coat protein complex II (COPII) export machinery...
  18. Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration.

    Journal of Biological Chemistry 284(36):24526 (2009) PMID 19561078 PMCID PMC2782044

    High grade gliomas such as glioblastoma multiforme express multiple members of the epithelial sodium channel (ENaC)/Degenerin family, characteristically displaying a basally active amiloride-sensitive cation current not seen in normal human astrocytes or lower grade gliomas. Using quantitative r...
  19. Evolutionary basis of converting a bacterial tRNA synthetase into a yeast cytoplasmic or mitochondrial enzyme.

    Journal of Biological Chemistry 284(36):23954 (2009) PMID 19574213 PMCID PMC2781989

    Previous studies showed that cytoplasmic and mitochondrial forms of yeast valyl-tRNA synthetase (ValRS) are specified by the VAS1 gene through alternative initiation of translation. Sequence comparison suggests that the yeast cytoplasmic (or mature mitochondrial) ValRS contains an N-terminal app...
  20. CYP719B1 is salutaridine synthase, the C-C phenol-coupling enzyme of morphine biosynthesis in opium poppy.

    Journal of Biological Chemistry 284(36):24432 (2009) PMID 19567876 PMCID PMC2782036

    Morphine is a powerful analgesic natural product produced by the opium poppy Papaver somniferum. Although formal syntheses of this alkaloid have been reported, the morphine molecule contains five stereocenters and a C-C phenol linkage that to date render a total synthesis of morphine commerciall...