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Journal of Immunological Methods

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  1. Mouse models of hepatitis B and delta virus infection.

    Journal of Immunological Methods 410:39 (2014) PMID 24631647

    Liver disease associated to persistent infection with the hepatitis B virus (HBV) continues to be a major health problem of global impact. Therapeutic regimens currently available can efficiently suppress HBV replication; however, the unique replication strategies employed by HBV permit the virus to...
  2. Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays.

    Journal of Immunological Methods 409:62 (2014) PMID 24801479 PMCID PMC4138252

    Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis of variables impacting assay performance, nor development of a standardized pro...
  3. An ELISA method to compute endpoint titers to Epstein-Barr virus and cytomegalovirus: application to population-based studies.

    Journal of Immunological Methods 408:64 (2014) PMID 24859346 PMCID PMC4098116

    Indirect fluorescence analysis (IFA), the gold standard for determining herpesvirus antibody titers, is labor-intensive and poorly suited for large population-based studies. The enzyme-linked immunosorbent assay (ELISA) is used widely for measuring antiviral antibodies but also suffers drawbacks suc...
  4. Colorimetric engineered immunoprobe for the detection and quantification of microcystins.

    Journal of Immunological Methods 406:124 (2014) PMID 24607607

    We propose to apply recombinant antibody technologies to the production of a bivalent colorimetric immunoprobe (scFv-AP) made of the so-called scFv fused to the alkaline phosphatase (AP) of Escherichia coli. Recombinant antibody technologies allow the development of specific probes with improved pro...
  5. A flow cytometric method for the analysis of macrophages in the vascular wall.

    Journal of Immunological Methods 396(1-2):33 (2013) PMID 23928493

    We describe a method for the isolation and subsequent flow cytometric analysis of macrophages from aortas of mice. Cell suspensions were prepared from thoracic aortas of male C57BL/6J mice by a combination of manual disruption, incubation in enzymatic digestion medium, and passage through a 70 μm ce...
  6. Publishers Note
    Author(s) unavailable

    Journal of Immunological Methods 392(1-2):v (2013)

  7. Multiplex serology of paraneoplastic antineuronal antibodies.

    Journal of Immunological Methods 391(1-2):125 (2013) PMID 23500780

    We developed a multiplex assay based on Luminex technology. Reaction of serial dilutions of six onconeural standard sera with microsphere-bound antigens showed lower limits of detection than with Western blotting. Using the six standard sera at a dilution of 1:200, the average within-run coefficient...
  8. Distribution and determinants of circulating complement factor H concentration determined by a high-throughput immunonephelometric assay.

    Journal of Immunological Methods 390(1-2):63 (2013) PMID 23376722

    We describe the development and validation of a high throughput nephelometric assay for fH. Reagents from a commercial radial immunodiffusion (RID) assay (The Binding Site) were adapted for use on the Siemens BNII high throughput nephelometric instrument. The assay was calibrated with a highly purif...
  9. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk.

    Journal of Immunological Methods 389(1-2):18 (2013) PMID 23279946

    We describe the development of five high-affinity monoclonal antibodies (dissociation constants below nM range) against Stx2 using a recombinant toxoid as an immunogen. These antibodies, designated Stx2-1, Stx2-2, Stx2-3, Stx2-4, and Stx2-5 are IgG1 or IgG2a heavy-chain subclass with kappa light-cha...
  10. Performance evaluation of the MBio Diagnostics point-of-care CD4 counter.

    Journal of Immunological Methods 387(1-2):107 (2013) PMID 23063690 PMCID PMC3529779

    We evaluate the performance of a prototype point-of-care device (POC) to quantify CD4 T cells from MBio Diagnostics, Inc. Whole blood samples, both venous and capillary (finger stick), were collected from known HIV-infected participants at the University of California, San Diego Antiviral Research C...