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Journal of Immunological Methods

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  1. Utilization of imaging flow cytometry to define intermediates of megakaryopoiesis in vivo and in vitro

    Journal of Immunological Methods (1997)

    Imaging flow cytometry is a particularly powerful analytical approach for the study of megakaryopoiesis. It can utilize well-defined immunophenotypic markers as well as assess maturation of megakaryocytes by their increasing ploidy as they endoreplicate. Imaging flow cytometry can also ass...
  2. Isolation of high-affinity, neutralizing anti-idiotype antibodies by phage and ribosome display for application in immunogenicity and pharma...

    Journal of Immunological Methods 416:49 (2015) PMID 25449532

    We have used a combination of phage and ribosome display to isolate a panel of monoclonal anti-idiotype antibodies with sub-nanomolar affinity and high specificity to a human anti-IgE monoclonal antibody. Anti-idiotype antibodies were enriched from scFv libraries using phage display, and a biochemic...
  3. Mouse models of hepatitis B and delta virus infection.

    Journal of Immunological Methods 410:39 (2014) PMID 24631647

    Liver disease associated to persistent infection with the hepatitis B virus (HBV) continues to be a major health problem of global impact. Therapeutic regimens currently available can efficiently suppress HBV replication; however, the unique replication strategies employed by HBV permit the virus to...
  4. Development and implementation of a proficiency testing program for Luminex bead-based cytokine assays.

    Journal of Immunological Methods 409:62 (2014) PMID 24801479 PMCID PMC4138252

    Luminex bead array assays are widely used for rapid biomarker quantification due to the ability to measure up to 100 unique analytes in a single well of a 96-well plate. There has been, however, no comprehensive analysis of variables impacting assay performance, nor development of a standardized pro...
  5. An ELISA method to compute endpoint titers to Epstein-Barr virus and cytomegalovirus: application to population-based studies.

    Journal of Immunological Methods 408:64 (2014) PMID 24859346 PMCID PMC4098116

    Indirect fluorescence analysis (IFA), the gold standard for determining herpesvirus antibody titers, is labor-intensive and poorly suited for large population-based studies. The enzyme-linked immunosorbent assay (ELISA) is used widely for measuring antiviral antibodies but also suffers drawbacks suc...
  6. Colorimetric engineered immunoprobe for the detection and quantification of microcystins.

    Journal of Immunological Methods 406:124 (2014) PMID 24607607

    We propose to apply recombinant antibody technologies to the production of a bivalent colorimetric immunoprobe (scFv-AP) made of the so-called scFv fused to the alkaline phosphatase (AP) of Escherichia coli. Recombinant antibody technologies allow the development of specific probes with improved pro...
  7. A halotyrosine antibody that detects increased protein modifications in asthma patients.

    Journal of Immunological Methods 403(1-2):17 (2014) PMID 24295867 PMCID PMC3944894

    We evaluated whether a 3-bromobenozoic acid hapten antigen produced antibodies that recognized halogenated tyrosine residues. Studies with small-molecule inhibitors or brominated or chlorinated protein suggested that a mouse monoclonal antibody (BTK-94C) selectively bound free and protein mono- and...
  8. A flow cytometric method for the analysis of macrophages in the vascular wall.

    Journal of Immunological Methods 396(1-2):33 (2013) PMID 23928493

    We describe a method for the isolation and subsequent flow cytometric analysis of macrophages from aortas of mice. Cell suspensions were prepared from thoracic aortas of male C57BL/6J mice by a combination of manual disruption, incubation in enzymatic digestion medium, and passage through a 70 μm ce...
  9. Publishers Note
    Author(s) unavailable

    Journal of Immunological Methods 392(1-2):v (2013)

  10. Multiplex serology of paraneoplastic antineuronal antibodies.

    Journal of Immunological Methods 391(1-2):125 (2013) PMID 23500780

    We developed a multiplex assay based on Luminex technology. Reaction of serial dilutions of six onconeural standard sera with microsphere-bound antigens showed lower limits of detection than with Western blotting. Using the six standard sera at a dilution of 1:200, the average within-run coefficient...