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  1. Effects of temperature on leaf hydraulic architecture of tobacco plants.

    Planta 240(3):489 (2014) PMID 24915747

    We grew plants of tobacco (Nicotiana tabacum) 'k326' in separate glasshouse rooms set to different day/night temperature conditions: low (LT 24/18 °C), medium (MT 28/22 °C), or high (HT 32/26 °C). After 40 days of such treatment, their leaf anatomies, leaf hydraulics, photosynthetic rates, and insta...
  2. Out-of-plane orientation of cellulose elementary fibrils on spruce tracheid wall based on imaging with high-resolution transmission electron...

    Planta 240(3):565 (2014) PMID 24965142

    A 3D model of the tracheid wall has been proposed based on high-resolution cryo-TEM where, in contrast to the current understanding, the cellulose elementary fibrils protrude from the cell wall plane. The ultrastructure of the tracheid walls of Picea abies was examined through imaging of ultrathin r...
  3. Age-dependent and jasmonic acid-induced laticifer-cell differentiation in anther callus cultures of rubber tree.

    Planta 240(2):337 (2014) PMID 24841475

    We present molecular and physiological evidences for laticifer-cell differentiation in calli derived from rubber tree anthers. RT-PCR analysis showed that three key genes rubber elongation factor (REF), small rubber particle protein (SRPP), and cis-prenyl transferase (CPT) that are essential in late...
  4. A DEAD-box RNA helicase produces two forms of transcript that differentially respond to cold stress in a cryophyte (Chorispora bungeana).

    Planta 240(2):369 (2014) PMID 24863059

    We characterized a cold-induced DEAD-box RNA helicase gene from C. bungeana. We cloned the full-length cDNA of the gene by RACE and called it C. bungeana DEAD-box RNA Helicase (CbDRH). Structurally, CbDRH possesses all nine conserved motifs characteristic of DEAD-box protein family members in its ce...
  5. Two aspartate residues at the putative p10 subunit of a type II metacaspase from Nicotiana tabacum L. may contribute to the substrate-bindin...

    Planta 239(1):147 (2014) PMID 24121807

    We report for the first time the identification and initial characterization of a metacaspase of Nicotiana tabacum L., NtMC1. By combining domain search, multiple sequence alignment (MSA), and protein fold-recognition studies, we provide compelling evidences that NtMC1 is a plant metacaspase type II...
  6. Role of asparaginase variable loop at the carboxyl terminal of the alpha subunit in the determination of substrate preference in plants.

    Planta 235(5):1013 (2012) PMID 22127737

    Structural determinants responsible for the substrate preference of the potassium-independent (ASPGA1) and -dependent (ASPGB1) asparaginases from Arabidopsis thaliana have been investigated. Like ASPGA1, ASPGB1 was found to be catalytically active with both L: -Asn and β-Asp-His as...
  7. Inhibition of aconitase in citrus fruit callus results in a metabolic shift towards amino acid biosynthesis.

    Planta 234(3):501 (2011) PMID 21528417

    We investigated the effect of citramalate and of another aconitase inhibitor, oxalomalate, on aconitase activity and regulation in callus originated from juice sacs. These compounds significantly increased citrate content and reduced the enzyme's activity, while slightly inducing its protein level....
  8. Decreased ROS level and activation of antioxidant gene expression in Agrobacterium rhizogenes pRiA4-transformed calli of Rubia cordifolia.

    Planta 232(5):1023 (2010) PMID 20680642

    We tested whether transformation of Rubia cordifolia cells by Agrobacterium rhizogenes had a similar effect. We isolated partial cDNA sequences of ascorbate peroxidase, catalase and Cu/Zn superoxide dismutase genes (RcApx1, RcApx2, RcApx3, RcCAT1, RcCAT2, RcCSD1, RcCSD2 and RcCSD3) from plant tissue...
  9. Sustained enhancement of photosynthesis in mature deciduous forest trees after 8 years of free air CO(2) enrichment.

    Planta 232(5):1115 (2010) PMID 20700744

    We investigated gas exchange characteristics and leaf traits in five deciduous tree species during their eighth growing season under free air carbon dioxide enrichment in a 35-m tall, ca. 100-year-old mixed forest. Net photosynthesis of upper-canopy foliage was 48% (July) and 42% (September) higher...
  10. Acclimation of photosynthesis to nitrogen deficiency in Phaseolus vulgaris.

    Planta 232(4):887 (2010) PMID 20632184

    We studied adjustments of the photosynthetic machinery in nitrogen-deficient bean plants and found four phenomena. First, the number of chloroplasts per cell decreased. Chloroplasts of nitrogen starved leaves contained less pigments than those of control leaves, but the in vitro activities of light...