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  1. Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey.

    Biotechnology Letters 30(10):1861 (2008) PMID 18575811

    Tissue-specific promoters are required for plant molecular breeding to drive a target gene in the appropriate location in plants. A chromoplast-specific, carotenoid-associated gene (OgCHRC) and its promoter (Pchrc) were isolated from Oncidium orchid and characterized. Northern blot analysis reve...
  2. Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica.

    Biotechnology Letters 30(10):1853 (2008) PMID 18575812

    Centella asiatica has been extensively studied but there has been no report to date that relates gene expression and centelloside production in non-differentiated tissues. We have determined the content of the four principal triterpenoid bioactive compounds of C. asiatica (asiaticoside, madecass...
  3. Cerulenin inhibits de novo sophorolipid synthesis of Candida bombicola.

    Biotechnology Letters 30(10):1829 (2008) PMID 18568403

    Synthesis of medium-chain sophorolipids by Candida bombicola is a challenging objective. One of the difficulties is that the obtained sophorolipids always represent a mixture of medium-chain and native de novo formed or long-chain sophorolipids. The fatty acid moiety of de novo sophorolipids is ...
  4. Biofuel cell system employing thermostable glucose dehydrogenase.

    Biotechnology Letters 30(10):1753 (2008) PMID 18516502

    Enzyme biofuel cells utilizing glucose dehydrogenase as an anode enzyme were constructed. The glucose dehydrogenase is composed of a catalytic subunit, an electron transfer subunit, and a chaperon-like subunit. Cells, constructed using either a glucose dehydrogenase catalytic subunit or a glucos...
  5. Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol.

    Biotechnology Letters 30(10):1807 (2008) PMID 18575813

    The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatal...
  6. Optimization of octanoic acid and sulfur donor concentrations for lipoic acid production by Pseudomonas reptilivora.

    Biotechnology Letters 30(10):1825 (2008) PMID 18575810

    Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1).
  7. Induced hydrolytic enzymes of ectomycorrhizal fungi against pathogen Rhizoctonia solani.

    Biotechnology Letters 30(10):1777 (2008) PMID 18574560

    Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the grow...
  8. Resolution of secondary alcohols via Carica papaya lipase-catalyzed enantioselective acylation.

    Biotechnology Letters 30(10):1783 (2008) PMID 18512020

    The Carica papaya lipase-catalyzed acylation of benzylcarbinols with vinyl hexanoate proceeded smoothly and enantiospecifically (E > 200), affording the R-esters and leaving the S-alcohols intact. Thus, this plant lipase proved to be a promising biocatalyst for the resolution of alcohols as well...
  9. Electricity generation in a microbial fuel cell with a microbially catalyzed cathode.

    Biotechnology Letters 30(10):1771 (2008) PMID 18563585

    A microbial fuel cell using aerobic microorganisms as the cathodic catalysts is described. By using anaerobic sludge in the anode and aerobic sludge in the cathode as inocula, the microbial fuel cell could be started up after a short lag time of 9 days, generating a stable voltage of 0.324 V (R ...
  10. Synthetic seed production from somatic embryos of Pinus radiata.

    Biotechnology Letters 30(10):1847 (2008) PMID 18563583

    Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for t...