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Biotechnology Letters

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  1. Inhibiting cyprinid herpesvirus-3 replication with CRISPR/Cas9.

    Biotechnology Letters (2015) PMID 26712370

    The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro.
  2. Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris.

    Biotechnology Letters 30(10):1801 (2008) PMID 18575809

    A synthetic gene coding for single-chain variable domain fragment antibody against mycotoxin zearalenone (scFv-ZEN) has been designed, constructed and expressed in Pichia pastoris. The native scFv-ZEN sequence was optimized to Pichia preference codon usage. The expression level of codon-optimize...
  3. What affects the innovation performance of small and medium-sized enterprises (SMEs) in the biotechnology industry? An empirical study on Korean biotech SMEs.

    Biotechnology Letters 30(10):1699 (2008) PMID 18506395

    Research-intensive small and medium-sized enterprises (SMEs) play a crucial role in the advancement of the biotechnology industry. This paper explored the impacts of internal and contextual variables on innovative activity in Korea and compared the results of this analysis with previous studies ...
  4. Biofuel cell system employing thermostable glucose dehydrogenase.

    Biotechnology Letters 30(10):1753 (2008) PMID 18516502

    Enzyme biofuel cells utilizing glucose dehydrogenase as an anode enzyme were constructed. The glucose dehydrogenase is composed of a catalytic subunit, an electron transfer subunit, and a chaperon-like subunit. Cells, constructed using either a glucose dehydrogenase catalytic subunit or a glucos...
  5. Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol.

    Biotechnology Letters 30(10):1807 (2008) PMID 18575813

    The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatal...
  6. Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase.

    Biotechnology Letters 30(10):1765 (2008) PMID 18563582

    HydE, HydF, and HydG participate in the synthesis of the complex di-iron center of [FeFe] hydrogenases. The hydE, hydF, hydG, hydA, and hydB genes of Desulfovibrio vulgaris Hildenborough were cloned and His-tag pull-down assays were used to study the potential interaction between HydE, HydF, and...
  7. Multiple gene disruptions by marker recycling with highly efficient gene-targeting background (DeltaligD) in Aspergillus oryzae.

    Biotechnology Letters 30(10):1811 (2008) PMID 18574559

    Previously we reported that double disruption of the proteinase genes (tppA and pepE) improved heterologous protein production by Aspergillus oryzae (Jin et al. Appl Microbiol Biotechnol 76:1059-1068, 2007). Since A. oryzae has 134 protease genes, the number of auxotrophy in a single host is lim...
  8. Application of bipolar electrodialysis on recovery of free lactic acid after simultaneous saccharification and fermentation of cassava starch.

    Biotechnology Letters 30(10):1747 (2008) PMID 18568401

    The efficiency of bipolar electrodialysis (BED) for the recovery of lactic acid from fermentation broth was evaluated. Three systems of BED (bipolar-anion, bipolar-cation and bipolar-anion-cation) at fixed voltage (20 V) were compared using a model solution of ammonium lactate (100 g l(-1)). Res...
  9. Induced hydrolytic enzymes of ectomycorrhizal fungi against pathogen Rhizoctonia solani.

    Biotechnology Letters 30(10):1777 (2008) PMID 18574560

    Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the grow...
  10. A simple structured model for recombinant IDShr protein production in Pichia pastoris.

    Biotechnology Letters 30(10):1727 (2008) PMID 18521539

    A simple structured model is proposed for the methanol production phase of the iduronate 2-sulphate sulfatase recombinant enzyme (IDShr) in Pichia patoris Mut(+). The model is mainly focused in oxidative stress phenomenon due to methanol consumption and based on extracellular experimental inform...