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Biotechnology Letters

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  1. Recent developments in use of 1-aminocyclopropane-1-carboxylate (ACC) deaminase for conferring tolerance to biotic and abiotic stress.

    Biotechnology Letters 36(5):889 (2014) PMID 24563292

    Ethylene is an essential plant hormone also known as a stress hormone because its synthesis is accelerated by induction of a variety of biotic and abiotic stress. The plant growth promoting bacteria containing the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase enhances plant growth by decr...
  2. Cloning and characterization of a protein elicitor Sm1 gene from Trichoderma harzianum.

    Biotechnology Letters 36(4):783 (2014) PMID 24322765

    We analyzed Sm1 gene expression of eight different strains of Trichoderma spp. grown on glucose, seeds or roots of beans. Regardless of the carbon source, T37 strain had significantly higher Sm1 expression and was chosen for further studies. When grown on different carbon sources, Sm1 expression was...
  3. Low temperature cell pausing: an alternative short-term preservation method for use in cell therapies including stem cell applications.

    Biotechnology Letters 36(2):201 (2014) PMID 24062136

    Encouraging advances in cell therapies have produced a requirement for an effective short-term cell preservation method, enabling time for quality assurance testing and transport to their clinical destination. Low temperature pausing of cells offers many advantages over cryopreservation, including t...
  4. Genetic engineering techniques for lactic acid bacteria: construction of a stable shuttle vector and expression vector for β-glucuronidase.

    Biotechnology Letters 36(2):327 (2014) PMID 24101246

    The shuttle vector, pUL6erm, was constructed by using a replicon from pL2, a multiple cloning site, colE1 ori, the ori of Gram-negative bacteria from vector pUC19, and the erythromycin resistance gene from pVA838 as a selection marker. pUL6erm could be transformed easily and maintained stably in Lac...
  5. Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.

    Biotechnology Letters 35(12):2081 (2013) PMID 23907669

    We used the double-stranded RNA binding domain (DRBD) that can bind to siRNA. The complex, consisting of PEI, siRNA and constructed fusion protein, TrxC2DRBD including C2 and DRBD domains, could protect siRNA from RNase degradation. Furthermore, cell specific siRNA delivery into HeLa cells could be...
  6. Expanding the landscape of recombinant protein production in Escherichia coli.

    Biotechnology Letters 35(12):1971 (2013) PMID 24170176

    Over the years, several vectors and host strains have been constructed to improve the overexpression of recombinant proteins in Escherichia coli. More recently, attention has focused on the co-expression of genes in E. coli, either by means of a single vector or by cotransformation with multiple com...
  7. Recent advances in glycotechnology for glycoconjugate synthesis using microbial endoglycosidases.

    Biotechnology Letters 35(11):1733 (2013) PMID 23801123

    Biotechnology associated with synthesis of glycopeptides and glycoproteins has recently advanced as glycotechnology. Studies toward glycotechonology include the artificial modification of sugar chains in glycoconjugates to improve their function because the physiological importance of sugar chains i...
  8. A wider role for polyamines in biofilm formation.

    Biotechnology Letters 35(11):1715 (2013) PMID 23881324

    Polyamines play an essential role in biofilm formation of diverse Gram-negative and Gram-positive bacteria. Biosynthetic pathways and transport systems for diverse polyamines have been identified as key components of bacterial biofilm formation.
  9. Apoptosis-antagonizing transcription factor (AATF) gene silencing: role in induction of apoptosis and down-regulation of estrogen receptor i...

    Biotechnology Letters 35(10):1561 (2013) PMID 23801113

    Apoptosis-antagonizing transcription factor (AATF) is involved in transcriptional regulation, cell cycle control, DNA damage responses and in the execution of cell death programs. It also interacts directly with nuclear hormone receptors to enhance their transactivation. This study highlights the RN...
  10. Production of sophorolipids with eicosapentaenoic acid and docosahexaenoic acid from Wickerhamiella domercqiae var. sophorolipid using fish ...

    Biotechnology Letters 35(6):901 (2013) PMID 23386226

    Sophorolipids (SLs) were synthesized by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 grown on fish oil and glucose. They were purified using preparative HPLC and their structures were identified by MS/MS. The yields of total and lactonic SLs were 47 and 19 g l(-1) in shake-flasks when fish...