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Biotechnology Letters

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  1. A new efficient expression system for Bacillus and its application to production of recombinant phytase

    Biotechnology Letters 22(16):1311 (2000)

  2. A dynamic membrane reactor with immobilized α-chymotrypsin for continuous kyotorphin synthesis in organic media

    Biotechnology Letters 22(9):771 (2000)

  3. Thermokinetic and energetic profiles of the yeast Debaryomyces hansenii in the presence of sodium chloride

    Biotechnology Letters 17(11):1233 (1995)

  4. A new system for the expression of recombinant antibody in mammalian cells

    Biotechnology Letters 17(2):135 (1995)

  5. Transitory plasmid instability during the exponential phase of growth of a recombinantEscherichia coli

    Biotechnology Letters 16(11):1113 (1994)

  6. A novel bacterial thermostable alpha-amylase system produced under solid state fermentation

    Biotechnology Letters 9(7):501 (1987)

  7. A comparison of genetically improved strains of the cellulolytic fungus Trichoderma reesei

    Biotechnology Letters 5(4):243 (1983)

  8. A novel one step process for cellulose fermentation using mesophilic cellulolytic and glycolytic Clostridia

    Biotechnology Letters 5(2):119 (1983)

  9. Expression of cold-adapted β-1,3-xylanase as a fusion protein with a ProS2 tag and purification using immobilized metal affinity chromatogra...

    Biotechnology Letters 37(1):89 (2015) PMID 25214227

    We confirmed that recombinant P.t.Xyn26A maintained its native fold. This is the first report of the expression of a cold-adapted enzyme fused with a ProS2 tag under IMAC purification using a high concentration of ArgHCl. These insights into the expression and purification should be useful during th...
  10. One-step integration of multiple genes into the oleaginous yeast Yarrowia lipolytica.

    Biotechnology Letters 36(12):2523 (2014) PMID 25216641

    We report a multiple fragment assembly method that allows one-step integration of an entire β-carotene biosynthesis pathway (~11 kb, consisting of four genes) via in vivo homologous recombination into the rDNA locus of the Y. lipolytica chromosome. The highest efficiency was 21 %, and the highest pr...