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- Ingenta, Rcgp, Proquest, Springer, Metapress, Gale, and CSA
Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein.
We used the double-stranded RNA binding domain (DRBD) that can bind to siRNA. The complex, consisting of PEI, siRNA and constructed fusion protein, TrxC2DRBD including C2 and DRBD domains, could protect siRNA from RNase degradation. Furthermore, cell specific siRNA delivery into HeLa cells could be...
In vitro evolution of glutathione S-transferase using a plasmid display system based on the GAL4 DNA-binding domain.
We describe a simple strategy based on plasmid display and limited proteolysis for the rapid and easy screening of highly stable and active mutants from a library. When glutathione S-transferase was used as a model enzyme, the resulting mutants obtained in the first round of screening were approxima...
Comparison of periplasmic and intracellular expression of Arabidopsis thionin proproteins in E. coli.
We tested the expression of all four Arabidopsis proproteins as fusion proteins. Periplasmic expression as fusion with maltose binding protein was not successful but cytoplasmic expression as His-tagged TRX fusion proteins with a TEV recognition sequence resulted in proteins of correct size. Use of...
Extraction of short-chain-length poly-[(R)-hydroxyalkanoates] (scl-PHA) by the "anti-solvent" acetone under elevated temperature and pressur...
A novel method was developed for extraction of short-chain-length poly(hydroxyalkanoates) (scl-PHA) from microbial biomass by the well-known "scl-PHA anti-solvent" acetone at elevated temperature and pressure in a closed system combining components for extraction, filtration, and product work-up. Re...
Nanoparticle-tethered NAD(+) with in situ cofactor regeneration.
A new and simple route for the preparation of immobilized NAD(+) on carboxyl-activated silica nanoparticles activated by γ-aminpropyltriethoxysilane and glutaric anhydride was developed. In addition, formate dehydrogenase, keto-reductase and the silica nanoparticle-attached NAD(+) were applied to ca...
Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli.
Genes of the key enzymes for phycocyanobilin (PCB) biosynthesis were cloned into E. coli and combinationally expressed to produce phycocyanobilin, with autologous heme as substrate. Culture conditions were optimized to achieve ~3 mg PCB/l. A protocol for the purification of recombinant phycocyanobil...
Modification of histidine biosynthesis pathway genes and the impact on production of L-histidine in Corynebacterium glutamicum.
We constructed plasmid pK18hisDPtac to replace the native hisD promoter with the tac promoter, and overexpressed phosphoribosyl-ATP-pyrophosphohydrolase, encoded by hisE, and ATP-phosphoribosyltransferase, encoded by hisG. The L-histidine titer at 0.85 g l(-1) was 80 % greater in the transformed bac...
Spirit-based distillers' grain as a promising raw material for succinic acid production.
Spirit-based distillers' grain (SDG) is the main by-product of the Chinese liquor industry, with an annual output of approx. 100 million tons. The economical potential of fermentative production of succinic acid from SDG was investigated using Actinobacillus succinogens. Use of pretreated SDG (PSDG)...
Eugenol inhibits quorum sensing at sub-inhibitory concentrations.
We confirmed that eugenol inhibited the las and pqs QS systems. Our data identified eugenol as a novel QS inhibitor. The purpose of this study was to track the quorum sensing inhibitor (QSI) in herbal extracts by effective screening systems and evaluate its biological activity. The QSIs from herbal...
Dimensionality reduction for microarray data using local mean based discriminant analysis.
We developed a new criterion for constructing the weight matrix by using local neighborhood information to discover the intrinsic discriminant structure in the data. Also this approach applies regularized least square technique to extract relevant features. We assess the performance of the proposed...