Biotechnology Letters

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  1. What affects the innovation performance of small and medium-sized enterprises (SMEs) in the biotechnology industry? An empirical study on Korean biotech SMEs.

    Biotechnology Letters 30(10):1699 (2008) PMID 18506395

    Research-intensive small and medium-sized enterprises (SMEs) play a crucial role in the advancement of the biotechnology industry. This paper explored the impacts of internal and contextual variables on innovative activity in Korea and compared the results of this analysis with previous studies ...
  2. Biofuel cell system employing thermostable glucose dehydrogenase.

    Biotechnology Letters 30(10):1753 (2008) PMID 18516502

    Enzyme biofuel cells utilizing glucose dehydrogenase as an anode enzyme were constructed. The glucose dehydrogenase is composed of a catalytic subunit, an electron transfer subunit, and a chaperon-like subunit. Cells, constructed using either a glucose dehydrogenase catalytic subunit or a glucos...
  3. Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase.

    Biotechnology Letters 30(10):1765 (2008) PMID 18563582

    HydE, HydF, and HydG participate in the synthesis of the complex di-iron center of [FeFe] hydrogenases. The hydE, hydF, hydG, hydA, and hydB genes of Desulfovibrio vulgaris Hildenborough were cloned and His-tag pull-down assays were used to study the potential interaction between HydE, HydF, and...
  4. Expression of functional single-chain variable domain fragment antibody (scFv) against mycotoxin zearalenone in Pichia pastoris.

    Biotechnology Letters 30(10):1801 (2008) PMID 18575809

    A synthetic gene coding for single-chain variable domain fragment antibody against mycotoxin zearalenone (scFv-ZEN) has been designed, constructed and expressed in Pichia pastoris. The native scFv-ZEN sequence was optimized to Pichia preference codon usage. The expression level of codon-optimize...
  5. Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol.

    Biotechnology Letters 30(10):1807 (2008) PMID 18575813

    The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatal...
  6. Multiple gene disruptions by marker recycling with highly efficient gene-targeting background (DeltaligD) in Aspergillus oryzae.

    Biotechnology Letters 30(10):1811 (2008) PMID 18574559

    Previously we reported that double disruption of the proteinase genes (tppA and pepE) improved heterologous protein production by Aspergillus oryzae (Jin et al. Appl Microbiol Biotechnol 76:1059-1068, 2007). Since A. oryzae has 134 protease genes, the number of auxotrophy in a single host is lim...
  7. Application of bipolar electrodialysis on recovery of free lactic acid after simultaneous saccharification and fermentation of cassava starch.

    Biotechnology Letters 30(10):1747 (2008) PMID 18568401

    The efficiency of bipolar electrodialysis (BED) for the recovery of lactic acid from fermentation broth was evaluated. Three systems of BED (bipolar-anion, bipolar-cation and bipolar-anion-cation) at fixed voltage (20 V) were compared using a model solution of ammonium lactate (100 g l(-1)). Res...
  8. Induced hydrolytic enzymes of ectomycorrhizal fungi against pathogen Rhizoctonia solani.

    Biotechnology Letters 30(10):1777 (2008) PMID 18574560

    Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the grow...
  9. A simple structured model for recombinant IDShr protein production in Pichia pastoris.

    Biotechnology Letters 30(10):1727 (2008) PMID 18521539

    A simple structured model is proposed for the methanol production phase of the iduronate 2-sulphate sulfatase recombinant enzyme (IDShr) in Pichia patoris Mut(+). The model is mainly focused in oxidative stress phenomenon due to methanol consumption and based on extracellular experimental inform...
  10. Optimization of octanoic acid and sulfur donor concentrations for lipoic acid production by Pseudomonas reptilivora.

    Biotechnology Letters 30(10):1825 (2008) PMID 18575810

    Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1).
  11. Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease.

    Biotechnology Letters 30(10):1839 (2008) PMID 18604480 PMCID PMC2522325

    Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the hu...
  12. Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice.

    Biotechnology Letters 30(10):1833 (2008) PMID 18563580

    Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic r...
  13. Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey.

    Biotechnology Letters 30(10):1861 (2008) PMID 18575811

    Tissue-specific promoters are required for plant molecular breeding to drive a target gene in the appropriate location in plants. A chromoplast-specific, carotenoid-associated gene (OgCHRC) and its promoter (Pchrc) were isolated from Oncidium orchid and characterized. Northern blot analysis reve...
  14. Improvement of innate immune responses and defense activity in mitten crab (Eriocheir sinensis) by oral administration of beta-glucan.

    Biotechnology Letters 30(10):1721 (2008) PMID 18568402

    The beta-glucan (BG), extracted from Saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (Eriocheir sinensis) at 0, 1, 5 and 10 g BG/kg diet for 28 days, followed by a challenge with Vibrio mimicus by intramuscular injection. Growth, phenoloxidase, superoxide dismutas...
  15. Phosphate assimilation by Chlorella and adjustment of phosphate concentration in basal medium for its cultivation.

    Biotechnology Letters 30(10):1735 (2008) PMID 18566756

    Assimilation of phosphate by Chlorella pyrenoidosa was 0.81-8.1 mg PO(4)-P/g dry weight for heterotrophic cultures and 0.81-16.1 mg/g for mixotrophic cultures. Optimal carbon:phosphorous (C/P) ratios were 206:1-2060:1 and 103:1-2060:1 for heterotrophic and mixotrophic cultivations, respectively....
  16. Thionin Thi2.1 from Arabidopsis thaliana expressed in endothelial cells shows antibacterial, antifungal and cytotoxic activity.

    Biotechnology Letters 30(10):1713 (2008) PMID 18563581

    Thionins are plant antimicrobial peptides with antibacterial and antifungal activities. Thionin Thi2.1 cDNA from Arabidopsis thaliana was expressed in BVE-E6E7 bovine endothelial cell line and its activity was evaluated against Escherichia coli, Staphylococcus aureus, Candida albicans and differ...
  17. Secretion of human interferon-beta 1b by recombinant Lactococcus lactis.

    Biotechnology Letters 30(10):1819 (2008) PMID 18575814

    Interferon-beta has anti-viral, anti-proliferation and multifunctional immunomodulatory activities and shows promising clinical effects for treatment of inflammatory disorders. The recombinant human interferon-beta (huIFN-beta) 1b was expressed in the food-grade lactic acid bacterium, Lactococcu...
  18. Cerulenin inhibits de novo sophorolipid synthesis of Candida bombicola.

    Biotechnology Letters 30(10):1829 (2008) PMID 18568403

    Synthesis of medium-chain sophorolipids by Candida bombicola is a challenging objective. One of the difficulties is that the obtained sophorolipids always represent a mixture of medium-chain and native de novo formed or long-chain sophorolipids. The fatty acid moiety of de novo sophorolipids is ...
  19. Synthetic seed production from somatic embryos of Pinus radiata.

    Biotechnology Letters 30(10):1847 (2008) PMID 18563583

    Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for t...
  20. Cloning and expression of a prion protein (PrP) gene from Korean bovine (Bos taurus coreanae) and production of rabbit anti-bovine PrP antibody.

    Biotechnology Letters 30(10):1705 (2008) PMID 18574558

    A PrP gene, from a Korean bovine, exhibiting a nonsense and a missense polymorphism respectively at nucleotides 576 and 652 has been cloned. The latter resulted in Glu to Lys substitution at amino acid residue 218. After expression and purification of the recombinant bovine PrP (recBoPrP) with G...