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Journal of Neuroscience Methods

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  1. Reproducibility and relevance of future behavioral sciences should benefit from a cross fertilization of past recommendations and today's te...

    Journal of Neuroscience Methods 234:2 (2014)

    We discuss this ‘status quo’ and propose solutions to advance behavioral testing. • Examples are given to illustrate the enlarged discriminability of novel test methods....
  2. Fish in behavior research: Unique tools with a great promise!

    Journal of Neuroscience Methods 234:54 (2014)

    • Comparative behavioral neuroscience answers phenogenetic and phylogenetic questions. • Study of new species increases translational relevance in biomedical research....
  3. A method for measuring brain partial pressure of oxygen in unanesthetized unrestrained subjects: the effect of acute and chronic hypoxia on ...

    Journal of Neuroscience Methods 193(2):217 (2010) PMID 20817029 PMCID PMC3044503

    We present a novel fiber optic method to measure brain PtO(2). This method can be used in unanesthetized, unrestrained animals, provides absolute values for PO(2), has a stable calibration, does not consume oxygen and is MRI compatible. Brain PtO(2) was studied during acute hypoxia, as well as befor...
  4. Assessing spatial vision - automated measurement of the contrast-sensitivity function in the hooded rat.

    Journal of Neuroscience Methods 97(2):103 (2000) PMID 10788664

    We therefore designed an economical and rapid method to assess the hooded rat's CSF, using a computer monitor to display stimuli and an infrared touch screen to record responses. A six-alternative forced-choice task presented trials in which a sine-wave grating (S+), varying in spatial frequency and...
  5. Independent component analyses for quantifying neuronal ensemble interactions.

    Journal of Neuroscience Methods 94(1):141 (1999) PMID 10638821

    We describe applications of principal component analysis (PCA) and independent component analysis (ICA) (Cardoso J-F, Souloumiac A. IEE-Proc F 1993;140:362-70; Hyvarinen A, Oja E. Neural Comput 1997;9:1483-92; Lee TW, Girolami M, Sejnowski TJ. Neural Comp 1999;11:417-41) to neuronal ensemble data. S...
  6. A simple analytical method for determining the steady-state potential in models of geometrically complex neurons.

    Journal of Neuroscience Methods 82(2):123 (1998) PMID 9700684

    A method is presented for solving the cable equation for a spiking neuron below firing threshold or a nonspiking neuron of arbitrary geometry under constant stimulation. The neuron structure is considered as a tree composed of a set of cylinder cables of three types (terminal, intermediate and branc...
  7. Validation of video motion-detection scoring of forced swim test in mice.

    Journal of Neuroscience Methods 235:59 (2014) PMID 24992574

    We developed a simple MATLAB-based motion-detection method to quantify mice's activity in videos of FST. FST trials are video-recorded from a side view. Each pixel of the video is compared between subsequent video frames; if the pixel's color difference surpasses a threshold, a motion count is recor...
  8. Micro RNA detection in long-term fixed tissue of cortical glutamatergic pyramidal neurons after targeted laser-capture neuroanatomical micro...

    Journal of Neuroscience Methods 235:76 (2014) PMID 24992573 PMCID PMC4150832

    We detected several classes of sRNA, such as miRNAs that play key roles in brain neurodevelopmental and maintenance pathways, and hsa-mir-155 expression in neurons. Comparison with existing method: Our method is the first to combine the approaches for: laser-capture of pyramidal neurons from long-te...
  9. Hybrid voltage sensor imaging of eGFP-F expressing neurons in chicken midbrain slices.

    Journal of Neuroscience Methods 233:28 (2014) PMID 24906054

    We have adapted a hVoS system for a chicken midbrain slice preparation by combining genetically expressed farnesylated eGFP with dipicrylamine (DPA). Depending on the cellular potential, DPA is shifted in the membrane, resulting in quenching of eGFP fluorescence linearly to the membrane potential by...
  10. Sensory and cognitive neurophysiology in rats, Part 1: Controlled tactile stimulation and micro-ECoG recordings in freely moving animals.

    Journal of Neuroscience Methods 232:63 (2014) PMID 24820913

    We have developed a setup for rats that allows for controlled sensory input to an animal engaged in a task while recording both electrophysiological signals and behavioral output. We record electrophysiological signals using a novel high-density micro-electrocorticography (micro-ECoG) grid that cove...