Trends in Biotechnology
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What place for polyacrylamide in proteomics?
Trends in Biotechnology 19:3 (2001)We describe the progress made towards establishing 2D-PAGE as the optimal separation tool for proteomics research....
Novel vaccine technologies.
Nanotechnology-based restorative materials for dental caries management.
We discuss the current progress and future applications of functional nanoparticles incorporated in dental restorative materials as useful strategies to dental caries management. We also overview proposed antimicrobial and remineralizing mechanisms. Nanomaterials have great potential to decrease bio...
Bioremediation, an environmental remediation technology for the bioeconomy.
Bioremediation differs from other industrial biotechnologies in that, although bioremediation contractors must profit from the activity, the primary driver is regulatory compliance rather than manufacturing profit. It is an attractive technology in the context of a bioeconomy but currently has limit...
Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics.
Clinical translation of mesenchymal stem cells (MSCs) is leading to optimization of procedures for ex vivo expansion. Endogenous growth factors and fibrin scaffolds can be used to support MSC expansion and transplantation. Cell growth on a fibrin scaffold mimics the 3D environment of tissue and faci...
Plant biotechnology: research behind fences.
The government of Switzerland has responded to vandalism of field experiments with genetically modified (GM) crops by establishing a protected field site. This site will enable research groups to conduct experiments without having to install and pay for security measures. This could be a model for o...
Exterior design: strategies for redecorating the bacterial surface with small molecules.
Recombinant techniques for expressing heterologous proteins and sugars on the surface of bacteria have been known since the 1980s, and have proven useful in a variety of settings from biocatalysis to vaccinology. The past decade has also seen the emergence of novel methods that allow modification of...
Stem cell metabolic and spectroscopic profiling.
Stem cells offer great potential for regenerative medicine because they regenerate damaged tissue by cell replacement and/or by stimulating endogenous repair mechanisms. Although stem cells are defined by their functional properties, such as the potential to proliferate, to self-renew, and to differ...
Advances in shaking technologies.
Shaking bioreactors are the most frequently used reactor system for screening and process optimization on a small scale. Their success can be attributed to their simple and functional design, which make shaking systems suitable for a large number of cost-efficient parallel experiments. Recently repo...
Exploiting cell surface thiols to enhance cellular uptake.
We discuss how thiol groups (-SH) naturally present on the cell surface (exofacial thiols) can be used to enhance cellular association and internalization of various materials bearing thiol-reactive groups in their structure. We propose that such thiol modifications should be considered in future de...