Trends in Biotechnology
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Advances in fermentative biohydrogen production: the way forward?
We concentrate on fermentative hydrogen production and highlight some recently applied approaches, such as response surface methodology, different reactor configurations and organisms that have been used to maximize hydrogen production rates and yields. However, there are significant remaining barri...
High-throughput screens for fluorescent dye discovery
A recent screen of a combinatorial library of fluorescent compounds discovered fluorescent dyes that were able to distinguish myoblasts from differentiated myotubes. New fluorescent dyes that respond to biologically relevant changes in cell state or type are useful as stains in a wide variety of bio...
Genetic design: rising above the sequence
Genetic engineering has developed around technologies enabling the targeted in vitro recombination of DNA molecules found in living organisms. As a result, the development of new DNA molecules has been primarily focused on cloning strategies that allow their assembly from existing DNA fragments. As...
Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.
We will focus here on highlighting their possible contributions to the pDNA purification challenge. Diverse affinity applications and their advantages and disadvantages are discussed, as well as the most significant results and improvements in the challenging task of purifying plasmids....
Environmental protection: applying the precautionary principle and proactive regulation to biotechnology
Biotechnology is a broad field encompassing diverse disciplines from agriculture to zoology. Advances in research are occurring at a rapid pace, and applications that have broad implications socially, economically, ecologically and politically are emerging. Along with notable benefits, environmental...
Genomics and proteomics in process development: opportunities and challenges
Global gene expression profiling by genomic and proteomic analyses has changed the face of drug discovery and biological research in the past few years. The benefit of these technologies in the area of process development for recombinant protein production has been increasingly realized. This review...
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Realization of @b-lactamase as a versatile fluorogenic reporter