Trends in Biotechnology
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Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges.
Although single-cell oil (SCO) has been studied for decades, lipid production from lignocellulosic biomass has received substantial attention only in recent years as biofuel research moves toward producing drop-in fuels. This review gives an overview of the feasibility and challenges that exist in r...
Autonomous surveillance for biosecurity
Trends in Biotechnology (2013)• Maps autonomous surveillance solutions to biosecurity problems. • Explores three classes of biosecurity problem in vectors, plants, and water....
What place for polyacrylamide in proteomics?
Trends in Biotechnology 19:3 (2001)We describe the progress made towards establishing 2D-PAGE as the optimal separation tool for proteomics research....
Drug target miRNAs: chances and challenges.
miRNAs, short ribonucleic acid gene regulators, are increasingly popular drug targets. Traditionally 'undruggable' proteins can be targeted via their miRNA gene regulators, enabling the treatment of diseases that, at present, seem impossible to cure. However, addressing miRNAs requires innovation at...
Engineering redox balance through cofactor systems.
Redox balance plays an important role in the production of enzymes, pharmaceuticals, and chemicals. To meet the demands of industrial production, it is desirable that microbes maintain a maximal carbon flux towards target metabolites with no fluctuations in redox. This requires functional cofactor s...
Delivery of intrahemocoelic peptides for insect pest management.
The extensive use of chemical insecticides for insect pest management has resulted in insecticide resistance now being recorded in >500 species of insects and mites. Although gut-active toxins such as those derived from Bacillus thuringiensis (Bt) have been successfully used for insect pest manageme...
Towards storage of cells and gametes in dry form.
We review published data on cell/gamete lyophilization. Most studies have utilized the same established protocols for cryopreservation (storage in liquid nitrogen) as for cell lyophilization (dehydration of frozen samples by water sublimation). Surveying natural lyoprotectants, we suggest trehalose...
Potential of proton-pumping rhodopsins: engineering photosystems into microorganisms.
A wide range of proton-pumping rhodopsins (PPRs) have been discovered in recent years. Using a synthetic biology approach, PPR photosystems with different features can be easily introduced in nonphotosynthetic microbial hosts. PPRs can provide hosts with the ability to harvest light and drive the su...
Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding.
We discuss how the recent evolution of targeted mutagenesis and DNA insertion techniques based on tailor-made site-directed nucleases (SDNs) provides opportunities to overcome such limitations. Plant breeding companies are exploiting SDNs to develop a new generation of crops with new and improved tr...
Invoking ideology in the promotion of ecological risk assessment for GM crops.