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Single aromatic residue location alters nucleic acid binding and chaperone function of FIV nucleocapsid protein
Virus Research 193:39 (2014)• FIV NC–nucleic acid interactions are probed using ensemble and single molecule approaches. • Structure of FIV and HIV-1 NC differ primarily by location of highly bas...
Inhibition of Theiler's virus-induced apoptosis in infected murine macrophages results in necroptosis
Virus Research 195:177 (2015)• Theiler's virus induces apoptosis in murine macrophages through the intrinsic pathway reducing virus yields late in infection. • Inhibition of apoptosis led to the i...
Incorporation of conserved nucleoprotein into influenza virus-like particles could provoke a broad protective immune response in BALB/c mice...
Virus Research 195:35 (2015)We develop virus-like particles (VLPs) vaccine candidate for influenza protection. • The VLPs are composed of hemagglutinin, neuraminidase, matrix 1 and nucleoprotein. • Incorp...
Development of fetal and placental innate immune responses during establishment of persistent infection with bovine viral diarrhea virus.
We have previously shown that heifers infected experimentally with ncp BVDV type 2 on d. 75 of gestation had transient robust up-regulation of the type I interferon (IFN) stimulated genes (ISGs) 3-15 days after viral inoculation. Blood from persistently infected (PI) fetuses, collected 115 days post...
Regulation of hepatitis C virus translation initiation by iron: role of eIF3 and La protein.
We investigated the possible relationship among iron status, eIFs and HCV IRES-mediated translation in vitro. Using bicistronic reporter gene constructs carrying HCV IRES sequence, we found that the levels of intracellular iron were positively associated with the HCV IRES-dependent translation initi...
Serial passage of a street rabies virus in mouse neuroblastoma cells resulted in attenuation: potential role of the additional N-glycosylati...
We have established an attenuated variant of street rabies virus strain 1088, originating from a rabid woodchuck followed by 2 passages in the brains of suckling mice. The variant, 1088-N30, was well adapted to NA cells and highly attenuated in adult mice after intramuscular (i.m.) but not intracere...
Epithelial cells from goat oviduct are highly permissive for productive infection with caprine arthritis-encephalitis virus (CAEV).
These results suggest also that the use in in vitro fertilisation systems of co-culture feeder cells that support efficient replication of CAEV to high titers could represent a serious risk for permanent transmission of virus to the cultured embryos and to the surrogate dam involved....
Sequence analysis of the turkey coronavirus nucleocapsid protein gene and 3' untranslated region identifies the virus as a close relative of...
The 3' end of the turkey coronavirus (TCV) genome (1740 bases) including the nucleocapsid (N) gene and 3' untranslated region (UTR) were sequenced and compared with published sequences of other avian and mammalian coronaviruses. The deduced sequence of the TCV N protein was determined to be 409 amino...
Primary and challenge infection of mice with equine herpesvirus 1, strain HSV25A.
Clinical signs, haematology, lymphocyte subset analysis, viral clearance, lung histopathology and humoral and cell-mediated (CMI) immune responses were monitored throughout the acute and convalescent phases of infection in groups of BALB/c mice infected intranasally with equine herpesvirus 1 (EHV-1)...
The position of the ZEBRA activation domain does not influence its biological activity.
We demonstrated that heterologous activation domains can fully substitute for the ZEBRA activation domain. Here we extend those results by showing that the position of the ZEBRA activation domain or a heterologous replacement domain does not influence its ability to function in the disruption of EBV...