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Long-term follow up of feline leukemia virus infection and characterization of viral RNA loads using molecular methods in tissues of cats wi...
Virus Research 197:137 (2015)• FeLV infection outcome is associated with viral RNA and provirus tissue loads. • Highest loads found at sites of virus replication and shedding....
Genetic variability of VP6, VP7, VP4, and NSP4 genes of porcine rotavirus group H detected in Brazil
Virus Research 197:48 (2015)• Detection of RVH in 9 Brazilian porcine stool samples. • Molecular characterization of VP6, VP7, VP4, and NSP4 genes of 6 RVH samples....
Changes in the mycovirus (LeV) titer and viral effect on the vegetative growth of the edible mushroom Lentinula edodes
Virus Research 197:8 (2015)• Fungal growth conditions affected the viral titer significantly. • Mycelial fragmentation method resulted in mycovirus LeV-cured L. edodes strains....
Interaction between phage BFK20 helicase gp41 and its host Brevibacterium flavum primase DnaG.
We tested the interactions between phage proteins gp40, gp41, gp42, gp43 and gp44 and between these phage proteins and host replication proteins DnaZX, DnaN, Dnaδ, DnaG, DnaA, RecF, TrxC, TrxB1 and SSB using a bacterial two-hybrid system. Phage proteins gp41, gp42 and gp43 self-associated, demonstra...
Controlled viral glycoprotein expression as a safety feature in a bivalent rabies-ebola vaccine
Virus Research 197:54 (2015)Our replication deficient rabies-ebolavirus bivalent vaccine candidate is capable of efficient infection in cell culture as evidenced by equivalent genome levels between the rabies virus glycoprotein trans-complementing cell line and target VeroE6 cells....
New evidence of replication of hepatitis C virus in short-term peripheral blood mononuclear cell cultures.
We have previously developed an innovative system where HCV-RNA can be recovered during PBMC culture from HCV infected patients. Thus, the aim of this work was to use this novel approach in order to observe the evolution and replication of HCV genotype 1b in the PBMC of an HIV-HCV coinfected patient...
Gallic acid-based small-molecule inhibitors of JC and BK polyomaviral infection.
We screened several compounds that possessed similar chemical space as sialic acid for their ability to bind the virus. Positive hits in the assay were restricted to gallic acid based compounds that mimic the viruses known cellular glycan receptors. Pre-treatment of virions with these inhibitors red...
The role of viral genomic RNA and nucleocapsid protein in the autophagic clearance of hantavirus glycoprotein Gn.
We previously reported that Gn is degraded shortly after synthesis by the host autophagy machinery. However, Gn being an important integral component of the virion, must escape degradation during the packaging and assembly stage of virus replication cycle. The mechanism regulating the intrinsic stea...
Grass carp reovirus induces apoptosis and oxidative stress in grass carp (Ctenopharyngodon idellus) kidney cell line.
Grass carp hemorrhage is an acute contagious disease caused by grass carp reovirus (GCRV). The pathogenesis of GCRV and the relationship between GCRV and the host cells remain unclear. The aim of the present study was to investigate the relations among apoptosis, intracellular oxidative stress and v...
Changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in Vero or C6/36 cells.
The aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of DENV-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. To this end, ten serial passages of DENV...