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[Antimicrobial activities of ant Ponericin W1 against plant pathogens in vitro and the disease resistance in its transgenic Arabidopsis].
These results demonstrate a potential use of Ponericin W1 in genetic engineering for broad-spectrum plant disease resistance....
[Mechanism on differential gene expression and heterosis formation].
We discussed the recent researches on allelic-specific expression in hybrid which was frequently observed in recent studies and analyzed its regulatory mechanism. All possible modes of gene action, including additivity, high- and low-parent dominance, underdominance, and over-dominance, were observe...
[Association of single nucleotide polymorphisms (SNPs) in leptin receptor gene with knee osteoarthritis in the Ningxia Hui population].
To investigate the association between primary knee osteoarthritis (OA) and single nucleotide polymorphism (SNP) (A668G) of leptin receptor gene (LEPR) in the Ningxia Hui population. A case-control association study has been adopted in this thesis. The polymerase chain reaction and restriction fragm...
[Differential expression analysis of proteins in neutrophils between clinical mastitis and healthy dairy cows].
Neutrophils provide the first line of defense against invading pathogens and have been reported to play a key role in bovine mammary immune. To examine the differential expression of proteins in neutrophils between clinical mastitis and healthy dairy cows, a 2-dimensional electrophor...
[Manual annotation of the pig whole genomic sequence using Otterlace software].
We used CFL1 (Cofilin 1) gene as an example to expound the usage of the three main components of Otterlace, Zmap, Blixem, and Dotter tools, and developed a practical procedure for manual annotations. We have analyzed 243 immune-related genes, among which 180 genes have been completely or partially a...
[Dosage compensation mechanism of X chromosome].
Dosage compensation mechanism is crucial for the balance expression of X chromosome genes, which ensures the protein or enzyme encoded by the X chromosome to be equal or almost equal expression amounts between males and females. However, different organisms have evolved distinct dosa...
[Application of next generation sequencing in microRNA detection].
MicroRNAs (miRNAs) are a class of ~22nt long non-coding RNAs. They are evolutionarily conserved and play essential roles in the regulation of post-transcriptional gene expression. The rapidly developing next generation sequencing (NGS) has important applications in miRNA detection. T...
[Application of case-based method in genetics and eugenics teaching].
We introduced case teaching method and got a better teaching effect. Based on our teaching practices, we summarized some experiences about this subject. In this article, the main problem of case-based method applied in Genetics and Eugenics teaching was discussed....
[Function of the Lck and Fyn in T cell development].
We discussed the mechanisms by which these two proteins perform functions in T cell development based on our current understanding....
[Molecular characteristics and specific PCR detection of transgenic rice containing Cry1Ab].
Bt01 is a new type of rice that has been genetically modified to express Cry1Abprotein. This study confirmed that Cry1Abwas inserted into Bt01 as a single copy using Southern blotting analysis. TAIL-PCR method was further used to obtain its insertion siteinformation. Specific PCR pri...