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The eubacterial protein synthesis inhibitor pulvomycin interacts with archaeal elongation factor 1α from Sulfolobus solfataricus.
The effect of pulvomycin on the biochemical and fluorescence spectroscopic properties of the archaeal elongation factor 1α from Sulfolobus solfataricus (SsEF-1α), the functional analog of eubacterial EF-Tu, was investigated. The antibiotic was able to reduce in vitro the rate of protein synthesis...
Mode of action of the antiprion drugs 6AP and GA on ribosome assisted protein folding.
We have characterized with biochemical experiments, the mode of inhibition of these two drugs using ribosomes or ribosomal components active in protein folding (referred to as 'ribosomal folding modulators' or RFMs) from both bacteria Escherichia coli and yeast Saccharomyces cerevisiae, and human ca...
A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae.
A chymotrypsin-like proteinase was isolated from the posterior midgut of larvae of the yellow mealworm, Tenebrio molitor, by ion-exchange and gel filtration chromatography. The enzyme, TmC1, was purified to homogeneity as determined by SDS-PAGE and postelectrophoretic activity detection. TmC1 had a...
Expression of eukaryotic glycosyltransferases in the yeast Pichia pastoris.
We have examined the expression in P. pastoris of Arabidopsis thaliana and Drosophila melanogaster core alpha1,3-fucosyltransferases (EC 188.8.131.52), A. thaliana beta1,2-xylosyltransferase (EC 184.108.40.206), bovine beta1,4-galactosyltransferase I (EC 220.127.116.11), D. melanogaster peptide O-xylosyltransferas...
The human TINF2 gene organisation and chromosomal localization.
We need to understand how the genes that control telomere length are regulated. TIN2, the protein product of the human TINF2 gene, was recently identified as a negative regulator of telomere length. Here I report the organisation and chromosomal localization of the TINF2 gene. It contains six exons...
Expression of the Daucus carota somatic embryogenesis receptor kinase (DcSERK) protein in insect cells.
The Daucus carota somatic embryogenesis receptor kinase (DcSERK) gene serves as marker to monitor the transition from somatic into embryogenic plant cells. To determine the intrinsic biochemical properties of the DcSERK protein, a predicted transmembrane receptor, the kinase domain was expressed as...
Colloidal aggregates of insoluble inclusions in human goiters.
We have screened retrospectively the autoradiographs of 60 human nodular goiters labeled 17 h preoperatively with 100 microCi 125I for evidence of colloid compartmentalization. In 87% (52/60) of all goiters examined, sporadic or multiple colloidal inclusions ('colloid stones') not mixing with newly...
Effects of thyroid-stimulating hormone on the replicative cycle of vesicular stomatitis virus in primary cultured sheep thyroid cells.
We infected thyroid cells with vesicular stomatitis virus (VSV) in the course of studies on cell polarity, and we found that TSH augmented the speed of the replicative cycle of VSV but did not affect the final yield of the virus. Three hours post-infection, at a multiplicity of infection of 10, the...
An adenosine receptor agonist-induced modulation of TSH-dependent cell growth in FRTL-5 thyroid cells mediated by inhibitory G protein, Gi.
We have now reexamined our preliminary finding of the inhibitory action of a non-metabolizable adenosine derivative, N6-(L-2-phenylisopropyl)adenosine (PIA), on the TSH-induced DNA synthesis to clarify the adenosine-dependent mechanism of cell growth modulation. PIA dose-dependently inhibited the TS...
Identification and characterization of mRNAs differentially expressed in thyroid cells stimulated by a mitogenic treatment.
The aim of our work is to identify new genes and proteins involved in the control of the proliferation of thyroid cells as putative protooncogenes and antioncogenes. Several strategies are discussed. A first study has allowed to identify three new genes. Further search will use the differential disp...