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  1. Cytotoxic activity of chimeric protein PD-L4UWSCI(tr) does not appear be affected by specificity of inhibition mediated by anti-protease WSC...

    Biochimie 107 Pt B:385 (2014) PMID 25457104

    We have produced a variant of chimeric protein, named PD-L4UWSCI(tr), changing the inhibitory specificity of WSCI domain. The purpose of this approach was to check if the cytotoxicity of the chimeric protein was altered depending on the properties of protease inhibitor domain or by a different fold...
  2. ATDOF5.8 protein is the upstream regulator of ANAC069 and is responsive to abiotic stress.

    Biochimie 110:17 (2015) PMID 25572919

    We report that ANAC069 is a direct target gene of the ATDOF5.8 transcription factor. There are seven DOF motifs in the promoter of ANAC069, which is highly enriched in the promoter. Yeast one-hybrid (Y1H) assays showed that a Dof protein, ATDOF5.8, binds to the DOF motifs. The interaction between AT...
  3. Dose-response estrogen promotes osteogenic differentiation via GPR40 (FFAR1) in murine BMMSCs.

    Biochimie 110:36 (2015) PMID 25576830

    We identified GPR40, a long chain unsaturated fatty acid receptor, to be regulated by estrogen and involved in osteogenic differentiation both in vivo and in vitro. Mechanistically, the Wnt/β-catenin signaling pathway is essential for GPR40 to promote osteogenic differentiation. Furthermore, in vivo...
  4. Exploring the aryl esterase catalysis of paraoxonase-1 through solvent kinetic isotope effects and phosphonate-based isosteric analogues of ...

    Biochimie 106:184 (2014) PMID 25180809

    We briefly present the solvent kinetic isotope effects on the phenyl acetate esterase activity of paraoxonase-1 and its inhibition with the phenyl methylphosphonate anion, which is a stable isosteric analogue that mimics the high-energy tetrahedral intermediate on the hydroxide-promoted hydrolysis p...
  5. A pilgrim's guide to G-quadruplex nucleic acid folding.

    Biochimie 105:1 (2014) PMID 25151411

  6. Characterization, mechanism of anticoagulant action, and assessment of therapeutic potential of a fibrinolytic serine protease (Brevithrombo...

    Biochimie 103:50 (2014) PMID 24735708

    In this study, biochemical and pharmacological characterization of Brevithrombolase, a fibrinolytic serine protease purified from Brevibacillus brevis strain FF02B has been reported. An assessment of its thrombolytic potency has also been made. The molecular mass of this monomeric protease was deter...
  7. Confusion between protectin D1 (PD1) and its isomer protectin DX (PDX). An overview on the dihydroxy-docosatrienes described to date.

    Biochimie 99:1 (2014) PMID 24262603

    We wish to point out that results are sometimes assigned to PD1 while they are indeed related to its isomer protectin DX (PDX) made through double lipoxygenation only. These misleading conclusions urge us to review herein the structural/chemical and biological differences in the docosatrienes report...
  8. Ubiquitin in the peroxisomal protein import pathway.

    Biochimie 98:29 (2014) PMID 23954799

    PEX5 is the shuttling receptor for newly synthesized peroxisomal matrix proteins. Alone, or with the help of an adaptor protein, this receptor binds peroxisomal matrix proteins in the cytosol and transports them to the peroxisomal membrane docking/translocation module (DTM). The interaction between...
  9. A tribute to Christian de Duve (1917-2013).

    Biochimie 98:1 (2014) PMID 24384473

  10. Temperature dependence of binding and catalysis for human serum arylesterase/paraoxonase.

    Biochimie 97:72 (2014) PMID 24096087

    The influence of temperature upon the hydrolysis of phenyl acetate, catalysed by purified human serum arylesterase/paraoxonase (E. C., was studied in the temperature range 10 °C-40 °C by spectrophotometry in TRIS buffer, pH 8.0, using both initial rate analysis and progress curve analysis....