Zeitschrift fur Naturforschung. Section C. Biosciences. Tubingen

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  1. A disappearance of a 24-kDa acid-soluble protein from liver chromatin of normal and starved hens following D-galactosamine administration.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(11-12):798 (1985) PMID 2421496

    Normal and starved adult chickens were injected intraperitoneally with D-galactosamine hydrochloride (0.5 g/kg body weight) and 6 h later liver chromatin acid-soluble proteins were isolated. These proteins were resolved by a two-dimensional polyacrylamide gel electrophoresis in the presence of n...
  2. Phosphorylation of coformycin and 2'-deoxycoformycin, and substrate and inhibitor properties of the nucleosides and nucleotides in several enzyme systems.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(9-10):710 (1985) PMID 3002059

    Under conditions where 2'-deoxycoformycin is enzymatically phosphorylated by wheat shoot phosphotransferase to the 5'-phosphate in 15-20% yield, coformycin is a relatively poor substrate, and is phosphorylated only to the extent of less than or equal to 5%. However, chemical phosphorylation of c...
  3. Chemical and physical characterization of four interfacial-active rhamnolipids from Pseudomonas spec. DSM 2874 grown on n-alkanes.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(1-2):51 (1985) PMID 3993180

    Four extracellular glycolipids produced under growth-limiting conditions were isolated from the culture broth of Pseudomonas spec. DSM 2874. After purification by column and thick-layer chromatography they were identified as anionic rhamnolipids. 1H and 13C-NMR studies showed that two of these, ...
  4. Acyl chain specificity and kinetic properties of phospholipase A1 and A2 of bone marrow-derived macrophages.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):356 (1985) PMID 4024704

    The fatty acyl specificity of phospholipase A1 and A2 in homogenates of mouse bone marrow-derived macrophages was determined using phosphatidylcholine and phosphatidylethanolamine of different acyl chain composition. Phosphatidylcholine with arachidonoyl at position 2 was cleaved preferentially ...
  5. Biochemical oxygen activation as the basis for the physiological action of tetrachlorodecaoxide (TCDO).

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):409 (1985) PMID 3839616

    Oxidation of methionine, 4-(methylthio)-2-oxobutyric acid (KMB), or 1-aminocyclopropane carbonic acid (ACC) are indicator reactions for activated oxygen species such as singlet oxygen (1O2), OH.-radical like oxidants, superoxide anion (O2.-), hydrogen peroxide (H2O2) or activated hemo-iron compl...
  6. Isolation of labeled lipoprotein from Escherichia coli and Proteus mirabilis after incubation with [14C]penicillin.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):415 (1985) PMID 3895763

    [14C]penicillin binding experiments and membrane analysis were carried out with cell envelope preparations from Escherichia coli and Proteus mirabilis. After incubation with [14C]penicillin G labeled free lipoprotein could be identified. The analysis of the isolated lipoprotein by SDS polyacryla...
  7. Localization of chromenes and benzofurans in the genus Encelia (Asteraceae).

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):301 (1985) PMID 4024703

    Phytochemical and microscopial analysis of leaves and stems of various species of Encelia showed a strict correlation between the presence of resin ducts and the accumulation of benzopyrans and benzofurans. Fluorescence microscopy of Encelia farinosa proved unambigously that these compounds are ...
  8. On the role of branched-chain amino acids in protein turnover of skeletal muscle. Studies in vivo with L-norleucine.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):427 (1985) PMID 3895764

    The effect of L-norleucine, an isomer of leucine, on protein metabolism in vivo was studied in suckling rats. Rats were injected subcutaneously with various doses of L-norleucine (0.5 and 5.0 mumol/g body wt.) every 12 h from 3 to 15 days post partum. Protein concentration, amino acid concentrat...
  9. Small-angle X-ray and light scattering studies on the influence of Mg2+ ions on the structure of the RNA from bacteriophage MS2.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(3-4):234 (1985) PMID 4002831

    The influence of Mg2+ ions on the secondary and tertiary structure of the RNA from bacteriophage MS2 was investigated by small-angle X-ray scattering and light scattering and by sedimentation experiments. The analysis of the outer part of the X-ray scattering curve obtained at low temperature in...
  10. Site of H atom attack on uracil and its derivatives in aqueous solution.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(3-4):292 (1985) PMID 4002833

    Hydrogen atoms from the radiolysis of water at pH 1.6 add to the 5,6-double bond of pyrimidines. The preferential site of attack is the C(5) position (values in brackets) in the case of 6-methyluracil (87%), 1,3-dimethyluracil (71%), uracil (69%) and poly(U) (60%). This reaction yields a radical...
  11. Properties of lectins from snails of the genus Helix probed by monoclonal antibodies.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(3-4):254 (1985) PMID 3923727

    Monoclonal antibodies were raised against the lectin of Helix pomatia (HPL). Besides antibodies bearing the more common gamma and kappa chains, antibodies with alpha, mu and lambda 2 chains were elicited. The anti-HPL antibodies are expected to be useful in studies on HPL biogenesis and HPL subs...
  12. Enhanced production of cell-bound and extracellular streptolysin S by hemolytic streptococci pretreated with proteases.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(3-4):166 (1985) PMID 3890397

    The amount of streptolysin S produced by resting streptococci was considerably increased after incubation of the washed bacteria with trypsin or pronase. Production of both cell-bound and free forms of the toxin was enhanced by the protease treatment. By addition of trypsin, streptolysin S yield...
  13. Kinetics of calcium-induced fusion of cell-size liposomes with monolayers in solutions of different osmolarity.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(1-2):92 (1985) PMID 3922148

    The effects of osmolarity, calcium concentration and cell-size liposomes in the subphase on the surface tension of phospholipid monolayers were investigated. The monolayers were spread from chloroform solutions of phosphatidic acid at air/water solution interface. The liposomes (of average diame...
  14. Purification and properties of adenosine kinase from rat liver: separation from deoxyadenosine kinase activity.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(1-2):34 (1985) PMID 2986372

    Ion exchange and affinity chromatography techniques, similar to those previously reported for purification of adenosine kinase from human placenta, were applied to purification of rat liver adenosine kinase. The enzyme, purified 400-fold in 41% yield, was homogeneous on SDS-polyacrylamide gel el...
  15. Phosphatidylinositol-specific phospholipase C from bovine blood platelets. Inhibition by calmodulin-inhibitors--activation by ATP and ADP.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(1-2):68 (1985) PMID 3993181

    The phospholipase C-activity in crude extracts of bovine blood platelets is strongly inhibited by the calmodulin-inhibitors fluphenazine and calmidazolium in the mM range, and activated by ATP and ADP, but not by AMP. The activating effect is also shown by the nonhydrolysable ATP- and ADP-analog...
  16. Occurrence of "stress"-proteins in yeast after heat-shock, acrylonitrile treatment and during the stationary growth phase.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(1-2):26 (1985) PMID 3887791

    The response of yeast cells to different kinds of "stress" is not identical. Cells of the stationary growth phase synthesize three new proteins of molecular weights 68, 27 and 24 kD, compared with cells of the exponential growth phase, while heat-shocked cells exhibit new proteins of 100, 90, 84...
  17. A small-angle X-ray scattering study on pre-irradiated malate synthase. The influence of formate, superoxide dismutase, and catalase on the X-ray induced aggregation of the enzyme.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):364 (1985) PMID 4024705

    The sulfhydryl enzyme malate synthase from baker's yeast was X-irradiated with 6 kGy in air-saturated aqueous solution (enzyme concentration: congruent to 10 mg/ml; volume: 120 microliters), in the absence or presence of the specific scavengers formate, superoxide dismutase, and catalase. After ...
  18. Prosomes are involved in the repression of viral mRNA.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(5-6):449 (1985) PMID 4024706

    Prosomes are small cytoplasmic RNP complexes. We present evidence that their RNA is a potential and selective inhibitor of viral mRNA translation while translation of normal cellular mRNA e.g. rabbit globin mRNA or HeLa cell mRNA is not affected.
  19. [Comparison of various immune surface labeling methods for scanning electron microscopy with the example of a surface antigen protein of the yeast Candida albicans].

    Zeitschrift fur Naturforschung. Section C. Bios... 40(7-8):539 (1985) PMID 3901566

    The labeling of immunocomplexes for scanning electron microscopy (SEM) is a fairly new technique, and the various procedures, that have been proposed, have not yet been compared. Such comparative evaluation was performed with Candida protease as a target antigen. This secretory enzyme of the opp...
  20. Reaction of fluorescein isothiocyanate with thiol and amino groups of sarcoplasmic ATPase.

    Zeitschrift fur Naturforschung. Section C. Bios... 40(11-12):863 (1985) PMID 2938353

    Several model compounds containing thiol and/or amino groups (mercaptoethanol, glutathione, cysteine, ethanolamine, glycine) were studied with respect to their reactivity towards fluorescein isothiocyanate (followed spectrophotometrically at 504 and 412 nm), stability of product and long-wave ab...