Zeitschrift fur Naturforschung. Section C. Biosciences. Tubingen
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In vitro antiproliferative and antifungal activity of essential oils from Erigeron acris L. and Erigeron annuus (L.) Pers.
Antiproliferative and antifungal activities of essential oils from Erigeron acris root and herb and from Erigeron annuus herb were investigated. The cell viability assay was performed in cultured fibroblasts, cancer cell lines (MCF-7 and MDA-MBA-231), and endometrial adenocarcinoma (...
Polymorphism of insulin-like growth factor I gene among chicken breeds in Egypt.
We describe how SSCP analysis of RT-PCR products can be used to evaluate the transcript expression pattern of avian IGF-I polymorphism, and their effect on the growth traits of chickens....
A disappearance of a 24-kDa acid-soluble protein from liver chromatin of normal and starved hens following D-galactosamine administration.
Normal and starved adult chickens were injected intraperitoneally with D-galactosamine hydrochloride (0.5 g/kg body weight) and 6 h later liver chromatin acid-soluble proteins were isolated. These proteins were resolved by a two-dimensional polyacrylamide gel electrophoresis in the presence of non-i...
[New isolation procedure for swine kidney acylase. Kinetics of Co2+, Mn2+, Ni2+ and Cd2+-enzymes].
A new procedure for the isolation of highly purified acylamino acid amidohydrolase from hog kidney is described which allows the preparation of the enzyme with a recovery of about 45%, a 200 fold purification and a spec. activity of 350-500 U. The essential Zn2+ of the enzyme was exchanged for Co2+,...
L-usnate-urease interactions: binding sites for polymerization.
L-usnic acid inactivates urease through a process which implicates the blockade of--SH groups in parallel to the formation of inactive polymers. Both L-alanine and L-proline partially reverses the inactivation and effectively diminishes the amount of highly polymerized protein. The amino acids also...
Electromechanical coupling III. Estimation of the Ca storage capacity of the SR by analysing the time course of caffeine-induced tension tra...
The Ca uptake of the SR of a skinned fibre, which was studied with an indirect method, shows a biphasic time course. A total Ca concentration between 8 and 23 mm was accumulated in the SR by an initial fast process (tau approximately equal to 4.7 s). This Ca-uptake activity of the SR is high enough...
Initiation of protein synthesis in yeast: binding of Met-tRNAi.
Conditions for the binding of Met-tRNAi to 40 s ribosomal subunits and to proteins isolated out of the yeast ribosomal KCl wash were investigated. Sucrose density gradient experiments revealed that binding of Met-tRNAi to 40 s ribosomal subunits was catalyzed in a AUG and GTP dependent reaction. Bin...
The specific labeling in hypertonic medium of a spleen protein.
The biosynthesis of 12 000 dalton protein species released into the medium by mouse spleen was resistant to the inhibitory effect of hypertonic medium on protein synthesis. This protein fraction comprised 10% or more of the total radioactive protein in the medium but could hardly be detected in the...
Altered heme environments in opossum and rabbit methemoglobins.
The effect of pH and inositol hexaphosphate (IHP) on the symmetry of the heme environments in opossum (Didelphius marsupialis) and new Zealand White rabbit hemoglobins has been studied using electron spin resonance (ESR). Each methemoglobin is found to contain two different heme environments as dete...
Paramagnetic species in beta-thalassemic sera: an ESR study.
We report that heme-human albumin complex shows a similar rhombic distortion. The observed ESR signal at g approximately equal to 6 is then attributed to this complex....