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- CSA, Ovid from 2000, Ingenta, Gale, Wiley from 1987, Rcgp, and Proquest
Shewanella oneidensis cytochrome c maturation component CcmI is essential for heme attachment at the non-canonical motif of nitrite reductas...
We identify SO0265 as CcmI, an apocytochrome c chaperone that is important and essential for maturation of c-type cytochromes with the canonical heme binding motif(s) (HBM; CX2 CH) and nitrite reductase NrfA carrying a non-canonical CX2 CK motif respectively. We show that the N-terminal transmembran...
CheY's acetylation sites responsible for generating clockwise flagellar rotation in Escherichia coli.
We identified K91 and K109 as the major sites whose acetylation level in vivo increases in response to acetate. Employing single and multiple lysine replacements in CheY, we found that K91 and K109 are also the sites mainly responsible for acetate-dependent clockwise generation. Furthermore, we show...
Specific N-terminal cleavage of ribosomal protein L27 in Staphylococcus aureus and related bacteria.
We demonstrate that L27 in Staphylococcus aureus and other Firmicutes is encoded with an N-terminal extension that is not present in most Gram-negative organisms and is absent from mature ribosomes. We have identified a cysteine protease, conserved among bacteria containing the L27 N-terminal extens...
The atypical response regulator HP1021 controls formation of the Helicobacter pylori replication initiation complex.
We found that Helicobacter pylori oriC activity is controlled by HP1021, a member of the atypical response regulator family. HP1021 protein specifically interacts with H. pylori oriC at HP1021 boxes (5'-TGTT[TA]C[TA]-3'), which overlap with three modules important for oriC function: DnaA boxes, the...
Steve Harris appointed editor for molecular biology and pathogenesis of fungi.
Vancomycin tolerance in clinical and laboratory Streptococcus pneumoniae isolates depends on reduced enzyme activity of the major LytA autol...
We examined two vancomycin-tolerant clinical isolates, i.e., a purported autolysin negative (LytA(-)), serotype 23F isolate (strain S3), and the serotype 14 strain "Tupelo" which is considered a paradigm of vancomycin tolerance. S3 was characterized here as carrying a frameshift mutation in the lytA...
The SciZ protein anchors the enteroaggregative
Type VI secretion sys...
We recently launched a study on the Sci-1 T6SS of enteroaggregative Escherichia coli (EAEC). The Sci-1 T6SS is composed of all or a subset of the 21 gene products encoded within the cluster, 13 of which are shared by all T6SS identified so far. In the present work, we focussed our attention on the S...
The molecular mechanism of mitochondria autophagy in yeast
We review the current knowledge on mitophagy, in particular on the molecular mechanism and regulation of mitophagy in yeast. We also discuss some of the differences between yeast and mammalian mitophagy....
Nuclear-encoded DnaJ homologue of
interacts with replication
We analyzed DNA-protein interactions at a plDNA replication ori region and report the identification of a nuclear-encoded DnaJ homolog that binds directly to ori elements of the plDNA molecule. PfDnaJ(A) interacted with the minor groove of the DNA double-helix and recognized a 13 bp sequence within...