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Phenolic Compounds Involved in Grafting Incompatibility of Vitis spp: Development and Validation of an Analytical Method for their Quantific...
Graft incompatibility of Vitis spp is an unresolved worldwide problem with important economic consequences. Grafting comprises a complex set of morphological and physiological alterations, in which the phenolic compounds seem to be strongly involved. Therefore, a detailed analysis and recognition of...
Advances in Isoflavone Profile Characterisation using Matrix Solid-phase Dispersion Coupled to HPLC/DAD in Medicago Species.
Analytical methods used in phytochemistry analysis are limited by the sample preparation step, which should ideally be fast, accurate, ecofriendly and achievable using low quantities of the sample. Matrix solid-phase dispersion (MSPD) may be a good alternative for combining extraction and purificati...
Vibrational spectroscopy as a rapid quality control method for Melaleuca alternifolia cheel (tea tree oil).
Tea tree oil (TTO) is an important commercial oil which has found application in the flavour, fragrance and cosmetic industries. The quality is determined by the relative concentration of its major constituents: 1,8-cineole, terpinen-4-ol, α-terpineol, α-terpinene, terpinolene, γ-terpinene and limon...
Purification of Phenylalkanoids and monoterpene glycosides from Rhodiola rosea L. roots by high-speed counter-current chromatography.
Rhodiola rosea L. is a medicinal herb used for its adaptogenic properties. The main active components are the phenylpropanoids collectively referred to as rosavins. To develop an isolation method for phytochemicals present in Rhodiola rosea roots using high-speed counter-current chromatography (HSCC...
Identification of the anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MS(n) and HPLC coupled with a post-column derivatisation system.
Flos Chrysanthemi (Jiju) is a traditional Chinese medicine (TCM) that is known to have anti-oxidant activity; in this study, on-line HPLC-DAD-ESI/MS(n) and HPLC-DAD-DPPH methods have been developed for rapidly screening and identifying free-radical scavengers in Jiju extract. To develop an efficient...
Multi-phase extraction of glycoraphanin from broccoli using aminium ionic liquid-based silica.
Glucosinolates, a class of phytochemicals found in broccoli, have attracted recent interest due to the potential health benefits associated with their dietary intake. Glucoraphanin, the most common glucosinolate in broccoli can be converted to a known cancer chemopreventive agent. Multi-phase extrac...
Molecular weight determination and correlation analysis of Dalbergia sissoo polysaccharide with constituent oligosaccharides.
Mucilaginous polysaccharide extracted from Dalbergia sissoo Roxb. leaves has a number of medicinal applications. Molecular weight studies and correlation analysis of the structure of polysaccharide with oligosaccharides can be helpful for further utilisation, modification and structure-activity rela...
Simple and reliable methods for the determination of three steroidal glycosides in the eight species of Solanum by reversed-phase HPLC coupl...
Solanum species are important ingredients of many traditional Indian medicines and thus the quality control of their herbal formulations is of paramount concern. To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate the quality of Solanum species and t...
An improved CTAB-ammonium acetate method for total RNA isolation from cotton.
We used phenol-chloroform and chloroform-isoamyl alcohol to remove proteins, polysaccharides and polyphenols, and ammonium acetate to precipitate RNA, reducing the incubation time prior to RNA precipitation. After adding ammonium acetate to precipitate RNA, all centrifugation steps (14000 × g) were...
Structure characterization and identification steroidal saponins from Ophiopogon japonicus Ker-Gawler (Liliaceae) by high-performance liquid...
Steroidal saponins are the main active constituents in Ophiopogon japonicus Ker-Gawler (Liliaceae). However, because of their high polarity, non-chromophores and low content in plants, steroidal saponins are difficult to be isolated from O. japonicus by conventional phytochemical methods. To develop...