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Phytochemical Analysis

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Wiley from 1991, Ingenta, Proquest, Rcgp, and CSA

  1. Vibrational spectroscopy as a rapid quality control method for Melaleuca alternifolia cheel (tea tree oil).

    Phytochemical Analysis 25(1):81 (2014) PMID 23934710

    Tea tree oil (TTO) is an important commercial oil which has found application in the flavour, fragrance and cosmetic industries. The quality is determined by the relative concentration of its major constituents: 1,8-cineole, terpinen-4-ol, α-terpineol, α-terpinene, terpinolene, γ-terpinene and limon...
  2. Purification of Phenylalkanoids and monoterpene glycosides from Rhodiola rosea L. roots by high-speed counter-current chromatography.

    Phytochemical Analysis 24(2):129 (2013) PMID 22811209

    Rhodiola rosea L. is a medicinal herb used for its adaptogenic properties. The main active components are the phenylpropanoids collectively referred to as rosavins. To develop an isolation method for phytochemicals present in Rhodiola rosea roots using high-speed counter-current chromatography (HSCC...
  3. Identification of the anti-oxidants in Flos Chrysanthemi by HPLC-DAD-ESI/MS(n) and HPLC coupled with a post-column derivatisation system.

    Phytochemical Analysis 24(1):59 (2013) PMID 22736398

    Flos Chrysanthemi (Jiju) is a traditional Chinese medicine (TCM) that is known to have anti-oxidant activity; in this study, on-line HPLC-DAD-ESI/MS(n) and HPLC-DAD-DPPH methods have been developed for rapidly screening and identifying free-radical scavengers in Jiju extract. To develop an efficient...
  4. Multi-phase extraction of glycoraphanin from broccoli using aminium ionic liquid-based silica.

    Phytochemical Analysis 24(1):81 (2013) PMID 22777845

    Glucosinolates, a class of phytochemicals found in broccoli, have attracted recent interest due to the potential health benefits associated with their dietary intake. Glucoraphanin, the most common glucosinolate in broccoli can be converted to a known cancer chemopreventive agent. Multi-phase extrac...
  5. Molecular weight determination and correlation analysis of Dalbergia sissoo polysaccharide with constituent oligosaccharides.

    Phytochemical Analysis 24(1):75 (2013) PMID 22786831

    Mucilaginous polysaccharide extracted from Dalbergia sissoo Roxb. leaves has a number of medicinal applications. Molecular weight studies and correlation analysis of the structure of polysaccharide with oligosaccharides can be helpful for further utilisation, modification and structure-activity rela...
  6. Simple and reliable methods for the determination of three steroidal glycosides in the eight species of Solanum by reversed-phase HPLC coupl...

    Phytochemical Analysis 24(1):87 (2013) PMID 22786841

    Solanum species are important ingredients of many traditional Indian medicines and thus the quality control of their herbal formulations is of paramount concern. To establish a simple and effective high-performance liquid chromatographic (HPLC) method to evaluate the quality of Solanum species and t...
  7. An improved CTAB-ammonium acetate method for total RNA isolation from cotton.

    Phytochemical Analysis 23(6):647 (2012) PMID 22552877

    We used phenol-chloroform and chloroform-isoamyl alcohol to remove proteins, polysaccharides and polyphenols, and ammonium acetate to precipitate RNA, reducing the incubation time prior to RNA precipitation. After adding ammonium acetate to precipitate RNA, all centrifugation steps (14000 × g) were...
  8. Structure characterization and identification steroidal saponins from Ophiopogon japonicus Ker-Gawler (Liliaceae) by high-performance liquid...

    Phytochemical Analysis 22(2):166 (2011) PMID 21046684

    Steroidal saponins are the main active constituents in Ophiopogon japonicus Ker-Gawler (Liliaceae). However, because of their high polarity, non-chromophores and low content in plants, steroidal saponins are difficult to be isolated from O. japonicus by conventional phytochemical methods. To develop...
  9. Determination of hydroxylated fatty acids from the biopolymer of tomato cutin and their fate during incubation in soil.

    Phytochemical Analysis 21(6):582 (2010) PMID 20799277

    The plant cuticle is a thin, predominantly lipid layer that covers all primary aerial surfaces of vascular plants. The monomeric building blocks of the cutin biopolymer are mainly ω-hydroxy fatty acids. Analysis of ω-hydroxy fatty acids from cutin isolated from tomato fruits at different stages of d...
  10. Rapid validated HPTLC method for estimation of betulinic acid in Nelumbo nucifera (Nymphaeaceae) rhizome extract.

    Phytochemical Analysis 21(6):556 (2010) PMID 21043041

    Betulinic acid (pentacyclic triterpenoid) is an important marker component present in Nelumbo nucifera Gaertn. rhizome. N. nucifera rhizome has several medicinal uses including hypoglycaemic, antidiarrhoeal, antimicrobial, diuretic, antipyretic, psychopharmacological activities. To establish a simpl...