Cell Transplantation

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  1. Beneficial role of pancreatic microenvironment for angiogenesis in transplanted pancreatic islets.

    Cell Transplantation 18(1):23 (2009) PMID 19476206

    Pancreatic islets implanted heterotopically (i.e., into the kidney, spleen, or liver) become poorly revascularized following transplantation. We hypothesized that islets implanted into the pancreas would become better revascularized. Islets isolated from transgenic mice expressing enhanced yello...
  2. Toward improving human islet isolation from younger donors: rescue purification is efficient for trapped islets.

    Cell Transplantation 18(1):13 (2009) PMID 19476205 PMCID PMC3777447

    Several reports suggest that islets isolated from younger donor pancreata are of better quality for clinical islet transplantation. The relative inefficiency of the continuous gradient purification process (CGP) is one of the major obstacles to the utilization of these younger donor pancreata. T...
  3. Improved survival of fulminant liver failure by transplantation of microencapsulated cryopreserved porcine hepatocytes in mice.

    Cell Transplantation 18(1):101 (2009) PMID 19476213

    The aim of this study was to establish hepatocyte isolation in pigs, and to evaluate function of isolated hepatocytes after encapsulation, cryopreservation, and transplantation (Tx) in a mouse model of fulminant liver failure (FLF). After isolation, porcine hepatocytes were microencapsulated wit...
  4. Assessments of proliferation capacity and viability of New Zealand rabbit peripheral blood endothelial progenitor cells labeled with superparamagnetic particles.

    Cell Transplantation 18(2):171 (2009) PMID 19499705

    Magnetic resonance imaging (MRI) has proven to be effective in tracking the distribution of transplanted stem cells to target organs by way of labeling cells with superparamagnetic iron oxide particles (SPIO). However, the effect of SPIO upon labeled cells is still unclear on a cellular level. W...
  5. Microtransplantation of dopaminergic cell suspensions: further characterization and optimization of grafting parameters.

    Cell Transplantation 18(2):119 (2009) PMID 19499701

    Intracerebral transplantation of dopaminergic (DA) cells is currently further explored as a potential restorative therapy for Parkinson's disease (PD). However, before they can be considered for a more widespread clinical use a number of critical issues have to be resolved, including an optimize...
  6. Collagenase isoforms for pancreas digestion.

    Cell Transplantation 18(2):203 (2009) PMID 19499708

    The available information concerning the characteristics and composition of collagenase batches, which are effective in the digestion of human pancreas for islet transplants, is scarce and incomplete. A large inter- and intrabatched variability in activity and efficiency of blend enzymes availab...
  7. Evaluation of a peritoneal-generated cardiac patch in a rat model of heterotopic heart transplantation.

    Cell Transplantation 18(3):275 (2009) PMID 19558776

    Tissue engineering holds the promise of providing new solutions for heart transplant shortages and pediatric heart transplantation. The aim of this study was to evaluate the ability of a peritoneal-generated, tissue-engineered cardiac patch to replace damaged myocardium in a heterotopic heart tr...
  8. NF-kappaB activity in endothelial cells is modulated by cell substratum interactions and influences chemokine-mediated adhesion of natural killer cells.

    Cell Transplantation 18(3):261 (2009) PMID 19558775 PMCID PMC2857529

    Because changes in subendothelial matrix composition are associated with alterations of the endothelial immune phenotype, we sought to understand if cytokine-induced NF-kappaB activity and downstream effects depend on substrate adherence of endothelial cells (EC). We compared the upstream phosph...
  9. Cardiac stem cell therapy: advances from 2008.

    Cell Transplantation 18(3):243 (2009) PMID 19558772

  10. Myoblast transfer in ischemic heart failure: effects on rhythm stability.

    Cell Transplantation 18(3):333 (2009) PMID 19558781

    Skeletal myoblast (SM) implantation promotes recovery of myocardial function after ischemic injury. Clinical observations suggest an association of SM implantation and ventricular arrhythmias. Support for this link has been sought in animal studies, but none employing models of congestive heart ...
  11. Implantation of a three-dimensional fibroblast matrix improves left ventricular function and blood flow after acute myocardial infarction.

    Cell Transplantation 18(3):283 (2009) PMID 19558777 PMCID PMC2739416

    This study was designed to determine if a viable biodegradable three-dimensional fibroblast construct (3DFC) patch implanted on the left ventricle after myocardial infarction (MI) improves left ventricular (LV) function and blood flow. We ligated the left coronary artery of adult male Sprague-Da...
  12. ERas is expressed in primate embryonic stem cells but not related to tumorigenesis.

    Cell Transplantation 18(4):381 (2009) PMID 19622226

    The ERas gene promotes the proliferation of and formation of teratomas by mouse embryonic stem (ES) cells. However, its human orthologue is not expressed in human ES cells. This implies that the behavior of transplanted mouse ES cells would not accurately reflect the behavior of transplanted hum...
  13. DAPI diffusion after intravitreal injection of mesenchymal stem cells in the injured retina of rats.

    Cell Transplantation 18(4):423 (2009) PMID 19622229

    To evaluate DAPI (4',6-diamidino-2-phenylindole) as a nuclear tracer of stem cell migration and incorporation it was observed the pattern of retinal integration and differentiation of mesenchymal stem cells (MSCs) injected into the vitreous cavity of rat eyes with retinal injury. For this purpos...
  14. Extracellular matrix in pancreatic islets: relevance to scaffold design and transplantation.

    Cell Transplantation 18(1):1 (2009) PMID 19476204 PMCID PMC2724969

    Intrahepatic islet transplantation provides a potentially more benign alternative to pancreatic transplantation. However, islet transplants are associated with limited engraftment potential. This inefficiency is likely at least partially attributable to the isolation process, which removes islet...
  15. Bone regeneration and neovascularization processes in a pellet culture system for periosteal cells.

    Cell Transplantation 18(4):443 (2009) PMID 19622231

    Reliable bone regeneration can be achieved with a pellet culture system using bovine periosteal cells. However, bone regeneration and neovascularization processes in this system have remained unclear. The present study aimed to clarify the extracellular environment and neovascularization process...
  16. Percutaneous cell delivery into the heart using hydrogels polymerizing in situ.

    Cell Transplantation 18(3):297 (2009) PMID 19558778 PMCID PMC2771541

    Heart disease is the leading cause of death in the US. Following an acute myocardial infarction, a fibrous, noncontractile scar develops, and results in congestive heart failure in more than 500,000 patients in the US each year. Muscle regeneration and the induction of new vascular growth to tre...
  17. Ex vivo nanofiber expansion and genetic modification of human cord blood-derived progenitor/stem cells enhances vasculogenesis.

    Cell Transplantation 18(3):305 (2009) PMID 19558779 PMCID PMC2749501

    The stem cell therapy for treating ischemic diseases is promising; however, the limited availability and compromised quality of progenitor cells in aged and diseased patients limit its therapeutic use. Here we report a nanofiber-based ex vivo stem cell expansion technology and proangiogenic grow...
  18. Retentive multipotency of adult dorsal root ganglia stem cells.

    Cell Transplantation 18(1):55 (2009) PMID 19476209 PMCID PMC2720448

    Preservation of neural stem cells (NSCs) in the adult peripheral nervous system (PNS) has recently been confirmed. However, it is not clear whether peripheral NSCs possess predestined, bona fide phenotypes or a response to innate developmental cues. In this study, we first demonstrated the longe...
  19. The effect of composite pig islet-human endothelial cell grafts on the instant blood-mediated inflammatory reaction.

    Cell Transplantation 18(1):31 (2009) PMID 19476207

    Instant blood-mediated inflammatory reaction (IBMIR) causes rapid islet loss in portal vein islet transplantation. Endothelial cells are known to protect against complement-mediated lysis and activation of coagulation. We tested composite pig islet-human endothelial cell grafts as a strategy to ...
  20. Reversible regulation of cell cycle-related genes by epigallocatechin gallate for hibernation of neonatal human tarsal fibroblasts.

    Cell Transplantation 18(4):459 (2009) PMID 19622233

    We investigated the hibernation effect of epigallocatechin-3-O-gallate (EGCG) on neonatal human tarsal fibroblasts (nHTFs) by analyzing the expression of cell cycle-related genes. EGCG application to culture media moderately inhibited the growth of nHTFs, and the removal of EGCG from culture med...