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Insect Biochemistry and Molecular Biology

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Ingenta, Gale, Proquest, CSA, Rcgp, and Sciencedirect from 1992

  1. Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella ger...

    Insect Biochemistry and Molecular Biology 33(10):1043 (2003) PMID 14505698

    I gene (COXI) was identified and isolated as a differentially expressed gene between insecticide susceptible ACY and resistant Apyr-R German cockroach strains using PCR-selected subtractive hybridization and cDNA array techniques. The cDNA sequence of COXI has an open reading frame of 1533 nucleotid...
  2. Prophenoloxidase-activating proteinase-3 (PAP-3) from Manduca sexta hemolymph: a clip-domain serine proteinase regulated by serpin-1J and se...

    Insect Biochemistry and Molecular Biology 33(10):1049 (2003) PMID 14505699

    We previously isolated PAP-1 from integument and PAP-2 from hemolymph of the tobacco hornworm, Manduca sexta. Here, we report the purification, characterization, and regulation of PAP-3 from the hemolymph. Similar to M. sexta PAP-2, PAP-3 consists of two amino-terminal clip domains followed by a car...
  3. Isolation, characterization and chemical synthesis of a new insect defensin from Chironomus plumosus (Diptera).

    Insect Biochemistry and Molecular Biology 28(12):1059 (1998) PMID 9887520

    We have isolated two 36-residue peptides from this hemolymph which are active against Gram-positive bacteria. The peptides are novel members of the insect defensin family and their sequences present marked differences with those of insect defensins isolated from other dipteran species. We have devel...
  4. A hydrophobic peptide (VAP-peptide) of the silkworm, Bombyx mori: structure, expression and an enhancing function of diapause hormone activi...

    Insect Biochemistry and Molecular Biology 28(2):75 (1998) PMID 9639873

    We have recently identified a unique lipophilic peptide (VAP-peptide) with diapause egg inducing activity in the silkworm, Bombyx mori (Imai et al., 1996). The cloning and sequencing of cDNA encoding VAP-peptide have demonstrated that the deduced amino acid sequence consisted of 84 amino acid residu...
  5. Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea).

    Insect Biochemistry and Molecular Biology 27(8-9):711 (1997) PMID 9443371

    We obtained three other cDNAs exhibiting high sequence similarity with Hyphantria cecropin A. We synthesized the C-terminally amidated peptide of 35 residues based on the deduced sequence of the isolated cDNA of Hyphantria cecropin A. The synthetic peptide exhibited strong antibacterial activity aga...
  6. Non conserved residues between Cqm1 and Aam1 mosquito α-glucosidases are critical for the capacity of Cqm1 to bind the Binary toxin from Lys...

    Insect Biochemistry and Molecular Biology 50:34 (2014) PMID 24746772

    The Binary (Bin) toxin from the entomopathogenic bacterium Lysinibacillus sphaericus acts on larvae of the culicid Culex quinquefasciatus through its binding to Cqm1, a midgut-bound α-glucosidase. Specific binding by the BinB subunit to the Cqm1 receptor is essential for toxicity however the toxin i...
  7. Insulin receptor-mediated nutritional signalling regulates juvenile hormone biosynthesis and vitellogenin production in the German cockroach...

    Insect Biochemistry and Molecular Biology 49:14 (2014) PMID 24657890

    We analysed the role of InR in different processes, but mainly those related to juvenile hormone (JH) synthesis and vitellogenin production. We first cloned the InR cDNA from B. germanica (BgInR) and then determined that its expression levels were constant in corpora allata and fat body during the f...
  8. Juvenile hormone signaling during reproduction and development of the linden bug, Pyrrhocoris apterus.

    Insect Biochemistry and Molecular Biology 45:69 (2014) PMID 24361539

    We employ the robust JH response during reproduction and development of the linden bug, Pyrrhocoris apterus, to compare the function of key JH-signaling genes encoding the JH receptor, Methoprene-tolerant (Met), its binding partner Taiman (Tai), and a JH-inducible protein, Krüppel-homolog 1 (Kr-h1)....
  9. Novel aquatic silk genes Simulium (Psilozia) vittatum (Zett) Diptera: Simuliidae.

    Insect Biochemistry and Molecular Biology 43(12):1181 (2013) PMID 24446544

    We identified three major silk gland proteins using a combined biochemical, proteomic, next-generation sequencing and bioinformatic approach. Biochemical characterization determined that they were phosphorylated with multiple isoforms and potentially differential phosphorylation. Structural characte...
  10. Ionotropic receptors (IRs): chemosensory ionotropic glutamate receptors in Drosophila and beyond.

    Insect Biochemistry and Molecular Biology 43(9):888 (2013) PMID 23459169

    We review the expression and function of IRs in Drosophila, highlighting similarities and differences with iGluRs. We also briefly describe the organization of the neuronal circuits in which IRs function, comparing and contrasting them with the sensory pathways expressing ORs. Finally, we summarize...