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Insect Biochemistry and Molecular Biology

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Ingenta, Gale, Proquest, CSA, Rcgp, and Sciencedirect from 1992

  1. Overexpression of the cytochrome c oxidase subunit I gene associated with a pyrethroid resistant strain of German cockroaches, Blattella ger...

    Insect Biochemistry and Molecular Biology 33(10):1043 (2003) PMID 14505698

    I gene (COXI) was identified and isolated as a differentially expressed gene between insecticide susceptible ACY and resistant Apyr-R German cockroach strains using PCR-selected subtractive hybridization and cDNA array techniques. The cDNA sequence of COXI has an open reading frame of 1533 nucleotid...
  2. Prophenoloxidase-activating proteinase-3 (PAP-3) from Manduca sexta hemolymph: a clip-domain serine proteinase regulated by serpin-1J and se...

    Insect Biochemistry and Molecular Biology 33(10):1049 (2003) PMID 14505699

    We previously isolated PAP-1 from integument and PAP-2 from hemolymph of the tobacco hornworm, Manduca sexta. Here, we report the purification, characterization, and regulation of PAP-3 from the hemolymph. Similar to M. sexta PAP-2, PAP-3 consists of two amino-terminal clip domains followed by a car...
  3. Isolation, characterization and chemical synthesis of a new insect defensin from Chironomus plumosus (Diptera).

    Insect Biochemistry and Molecular Biology 28(12):1059 (1998) PMID 9887520

    We have isolated two 36-residue peptides from this hemolymph which are active against Gram-positive bacteria. The peptides are novel members of the insect defensin family and their sequences present marked differences with those of insect defensins isolated from other dipteran species. We have devel...
  4. A hydrophobic peptide (VAP-peptide) of the silkworm, Bombyx mori: structure, expression and an enhancing function of diapause hormone activi...

    Insect Biochemistry and Molecular Biology 28(2):75 (1998) PMID 9639873

    We have recently identified a unique lipophilic peptide (VAP-peptide) with diapause egg inducing activity in the silkworm, Bombyx mori (Imai et al., 1996). The cloning and sequencing of cDNA encoding VAP-peptide have demonstrated that the deduced amino acid sequence consisted of 84 amino acid residu...
  5. Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea).

    Insect Biochemistry and Molecular Biology 27(8-9):711 (1997) PMID 9443371

    We obtained three other cDNAs exhibiting high sequence similarity with Hyphantria cecropin A. We synthesized the C-terminally amidated peptide of 35 residues based on the deduced sequence of the isolated cDNA of Hyphantria cecropin A. The synthetic peptide exhibited strong antibacterial activity aga...
  6. High resolution genetic mapping uncovers chitin synthase-1 as the target-site of the structurally diverse mite growth inhibitors clofentezin...

    Insect Biochemistry and Molecular Biology 51:52 (2014) PMID 24859419 PMCID PMC4124130

    We identified and investigated a Tetranychus urticae strain (HexR) harboring recessive, monogenic resistance to each of hexythiazox, clofentezine, and etoxazole. To elucidate if there is a common genetic basis for the observed cross-resistance, we adapted a previously developed bulk segregant analys...
  7. Two essential peritrophic matrix proteins mediate matrix barrier functions in the insect midgut.

    Insect Biochemistry and Molecular Biology 49:24 (2014) PMID 24680676

    We examined the physiological roles of 11 putative peritrophic matrix protein (PMP) genes of the red flour beetle, Tribolium castaneum (TcPMPs). TcPMP genes are differentially expressed along the length of the midgut epithelium of feeding larvae. RNAi of individual PMP genes revealed no abnormal dev...
  8. bmnpv-miR-3 facilitates BmNPV infection by modulating the expression of viral P6.9 and other late genes in Bombyx mori.

    Insect Biochemistry and Molecular Biology 49:59 (2014) PMID 24698834

    We show that bmnpv-miR-3, a Bombyx mori nucleopolyhedrovirus (BmNPV) encoded miRNA, regulates the expression of DNA binding protein (P6.9) and other late genes, vital for the late stage of viral infection in the host, Bombyx mori. We have performed both cell culture and in vivo experiments to establ...
  9. The maxillary palp of Aedes aegypti, a model of multisensory integration.

    Insect Biochemistry and Molecular Biology 48:29 (2014) PMID 24613607

    We surveyed the expressed genetic components and examined their potential involvement with these sensory modalities. Using Illumina sequencing, we identified the transcriptome of the maxillary palps of physiologically mature female Ae. aegypti. Genes expressed in the maxillary palps included those i...
  10. Induction of soluble AChE expression via alternative splicing by chemical stress in Drosophila melanogaster.

    Insect Biochemistry and Molecular Biology 48:75 (2014) PMID 24637386

    We generated a transgenic fly strain carrying the cDNA of DmAChE gene (Dm_ace) that predominantly expressed a single transcript variant encoding the membrane-anchored dimer. 3' RACE (rapid amplification of cDNA ends) and western blotting were performed to compare Dm_ace transcript variants and DmACh...