Journal of Structural Biology
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Large nuclear RNP particles—the nuclear pre-mRNA processing machine
Journal of Structural Biology in pressWe show that the cap-binding proteins (CBPs), CBP20 and CBP80, are associated with the lnRNP particles, as well as components of the 3′-end-processing activity. These results, together with our previous demonstration of the association of splicing factors and A-to-I editing enzymes with lnRNP part...
The distribution of RNA polymerase II largest subunit (RPB1) in the Xenopus germinal vesicle
Journal of Structural Biology in pressWe present evidence that this stain is due to cross-reaction with one or more SR proteins. We show that constructs consisting of 15–17 CTD heptapeptide repeats fused to glutathione-S-transferase are targeted rapidly and specifically to CBs but not to B-snurposomes after injection into the nucleus....
On the formation of amplified nucleoli during early Xenopus oogenesis
Journal of Structural Biology in pressWe have studied nucleolar development in early previtellogenic Xenopus oocytes. By using light and electron microscopic immunocytochemistry, in situ hybridization, and the TdT-method for detection of DNA, we have identified three separate structural entities which are related to the three components...
Single molecule imaging of supported planar lipid bilayer—reconstituted human insulin receptors by in situ scanning probe microscopy
Journal of Structural Biology in pressWe report here the results of an in situ study by high-resolution scanning probe microscopy of the full-length insulin receptor reconstituted within supported planar lipid bilayers. Our preliminary studies confirm that (1) the intact receptor can be reconstituted constitutively within a lipid vesicl...
Use of frozen-hydrated axonemes to assess imaging parameters and resolution limits in cryoelectron tomography
Journal of Structural Biology in pressWe have obtained tomographic reconstructions of frozen-hydrated sea urchin axonemes with 8–10-nm resolution, as assessed by detection of characteristic components including doublet microtubules, radial spokes, central sheath projections, and outer dynein arms. We did not detect the inner dynein ar...
Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery
A new chitinase-like agglutinin, RobpsCRA, related to family GH18 chitinases, has previously been identified in black locust (Robinia pseudoacacia) bark. The crystal structure of RobpsCRA at 1.85 Å resolution reveals unusual molecular determinants responsible for the lack of its ancestral...
The Cellulose System in the Cell Wall of Micrasterias
The cellulose system of the cell wall of Micrasterias denticulata and Micrasterias rotata was analyzed by diffraction contrast transmission electron microscopy, electron diffraction, and X-ray analysis. The studies, achieved on disencrusted cell ghosts, confirmed that the cellulose m...
Crystal structures of S-adenosylhomocysteine hydrolase from the thermophilic bacterium Thermotoga maritima
S-adenosylhomocysteine (SAH) hydrolase catalyzes the reversible hydrolysis of SAH into adenosine and homocysteine by using NAD+ as a cofactor. The enzyme from Thermotoga maritima (tmSAHH) has great potentials in industrial applications because of its hyperthermophilic properties. Here, two...