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Journal of Structural Biology

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  1. Coupling between inter-helical hydrogen bonding and water dynamics in a proton transporter.

    Journal of Structural Biology 186(1):95 (2014) PMID 24576681

    We explore the role of inter-helical hydrogen bonding in long-distance protein conformational coupling and dynamics of internal water molecules. From molecular dynamics simulations of wild type and nine different bacteriorhodopsin mutants we find that both intra- and inter-helical hydrogen bonds are...
  2. Local regularization of tilt projections reduces artifacts in electron tomography.

    Journal of Structural Biology 186(1):28 (2014) PMID 24632448

    We introduce a pre-reconstruction non-conservative non-linear isotropic diffusion (NID) filter that automatically identifies and reduces local irregularities in the tilt projections. We illustrate the improvement in quality obtained using this approach for reconstructed tomograms generated from samp...
  3. Xmipp 3.0: an improved software suite for image processing in electron microscopy.

    Journal of Structural Biology 184(2):321 (2013) PMID 24075951

    We present Xmipp 3.0, a major release which introduces several improvements and new developments over the previous version. A central improvement is the concept of a project that stores the entire processing workflow from data import to final results. It is now possible to monitor, reproduce and res...
  4. Crystal structure of rat intestinal alkaline phosphatase--role of crown domain in mammalian alkaline phosphatases.

    Journal of Structural Biology 184(2):182 (2013) PMID 24076154

    We have cloned, expressed, purified and characterized IAPs from rat and cynomolgus monkey (rIAP and cIAP respectively) which are important pre-clinical species for drug metabolism studies. The recombinant rat and monkey enzymes expressed in Sf9 insect cells (IAP-Ic) were found to be glycosylated and...
  5. Mapping the road to recovery: the ClpB/Hsp104 molecular chaperone.

    Journal of Structural Biology 179(2):161 (2012) PMID 22659404

    We focus on one family of Hsp100s, the homologous ClpB and Hsp104 molecular chaperones that convey thermotolerance by resolubilising and rescuing proteins from aggregates. We explore how the nucleotide binding and hydrolysis properties at the twelve nucleotide-binding domains of these hexameric ring...
  6. Large nuclear RNP particles—the nuclear pre-mRNA processing machine
    Author(s) unavailable

    Journal of Structural Biology 2012

    We show that the cap-binding proteins (CBPs), CBP20 and CBP80, are associated with the lnRNP particles, as well as components of the 3′-end-processing activity. These results, together with our previous demonstration of the association of splicing factors and A-to-I editing enzymes with lnRNP part...
  7. The distribution of RNA polymerase II largest subunit (RPB1) in the Xenopus germinal vesicle
    Author(s) unavailable

    Journal of Structural Biology 2012

    We present evidence that this stain is due to cross-reaction with one or more SR proteins. We show that constructs consisting of 15–17 CTD heptapeptide repeats fused to glutathione-S-transferase are targeted rapidly and specifically to CBs but not to B-snurposomes after injection into the nucleus....
  8. On the formation of amplified nucleoli during early Xenopus oogenesis
    Author(s) unavailable

    Journal of Structural Biology 2012

    We have studied nucleolar development in early previtellogenic Xenopus oocytes. By using light and electron microscopic immunocytochemistry, in situ hybridization, and the TdT-method for detection of DNA, we have identified three separate structural entities which are related to the three components...
  9. Single molecule imaging of supported planar lipid bilayer—reconstituted human insulin receptors by in situ scanning probe microscopy
    Author(s) unavailable

    Journal of Structural Biology 2012

    We report here the results of an in situ study by high-resolution scanning probe microscopy of the full-length insulin receptor reconstituted within supported planar lipid bilayers. Our preliminary studies confirm that (1) the intact receptor can be reconstituted constitutively within a lipid vesicl...
  10. Use of frozen-hydrated axonemes to assess imaging parameters and resolution limits in cryoelectron tomography
    Author(s) unavailable

    Journal of Structural Biology 2012

    We have obtained tomographic reconstructions of frozen-hydrated sea urchin axonemes with 8–10-nm resolution, as assessed by detection of characteristic components including doublet microtubules, radial spokes, central sheath projections, and outer dynein arms. We did not detect the inner dynein ar...