Journal of Structural Biology
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- Sciencedirect from 1990, Ingenta, Gale, Rcgp, Proquest, and CSA
Atomic force microscopy studies of APOBEC3G oligomerization and dynamics.
We address these questions here using atomic force microscopy (AFM) to directly image A3G protein in a free-state and in complexes with DNA, and using time-lapse AFM imaging to characterize the dynamics of A3G oligomers. We found that the formation of oligomers is an inherent property of A3G and tha...
Selection and orientation of different particles in single particle imaging.
The short pulses of X-ray free electron lasers can produce diffraction patterns with structural information before radiation damage destroys the particle. The particles are injected into the beam in random orientations and they should be identical. However, in real experimental conditions it is not...
Likelihood-based classification of cryo-EM images using FREALIGN.
We describe an implementation of maximum likelihood classification for single particle electron cryo-microscopy that is based on the FREALIGN software. Particle alignment parameters are determined by maximizing a joint likelihood that can include hierarchical priors, while classification is performe...
On the function of chitin synthase extracellular domains in biomineralization.
We investigate the assembly mechanism of the chitin synthase Ar-CS1 via its extracellular domain ArCS1_E22. The corresponding transmembrane protein ArCS1_E22TM accumulates in membrane fractions of the expression host Dictyostelium discoideum. Soluble recombinant ArCS1_E22 proteins can be purified as...
Structural and mechanical properties of the arthropod cuticle: Comparison between the fang of the spider Cupiennius salei and the carapace o...
Most biological materials are nanocomposites characterized by a multi-level structural hierarchy. Particularly, the arthropod cuticle is a chitin-based composite material where the mechanical properties strongly depend on both molecular chitin/protein properties, and the structural arrangement of ch...
Fast and accurate reference-free alignment of subtomograms.
We propose an efficient and accurate alignment algorithm via a generalized convolution theorem, which allows computation of a constrained correlation function using spherical harmonics. This formulation increases computational speed of rotational matching dramatically compared to rotation search in...
Hysteresis in swelling and in sorption of wood tissue.
We report measurements of the reversible moisture-induced orthotropic swelling/shrinkage strains. Hysteresis is observed when the swelling/shrinkage strain is considered as a function of relative humidity, except for the very high porosity sample. Hysteresis is no longer present when swelling/shrink...
Marker-free dual-axis tilt series alignment.
We report on the extension of marker-free image registration based on cross-correlation techniques to dual-axis tilt series. A simultaneous geometry refinement yields accurate parameters for the computation of the final reconstruction. Both, image registration and 3D-reconstruction operate on the co...
The backbone model of the Arabis mosaic virus reveals new insights into functional domains of Nepovirus capsid.
We determined the 6.5 Å resolution cryo-electron microscopy structure of ArMV and used homology modeling and flexible fitting approaches to build its pseudo-atomic structure. This study allowed us to resolve ArMV CP architecture and delineate connections between ArMV capsid shell and its RNA. Compar...
Structural characterization of a D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus.
We report the apo and coenzyme NAD(+) complexed crystal structures of the L. bulgaricusD-isomer specific 2-hydroxyacid dehydrogenase, D2-HDH. Comparison with closely related members of the NAD-dependent dehydrogenase family reveals that whilst the D2-HDH core fold is structurally conserved, the subs...