Journal of Biomedical Optics

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  1. Publisher’s Note: Three-dimensional anterior segment imaging in patients with type 1 Boston Keratoprosthesis with switchable full depth range swept source optical coherence tomography

    Journal of Biomedical Optics 18(8):089802 (2013) PMCID PMC3763930

  2. Quantitative imaging of scattering changes associated with epithelial proliferation, necrosis, and fibrosis in tumors using microsampling reflectance spectroscopy.

    Journal of Biomedical Optics 14(1):014004 (2009) PMID 19256692 PMCID PMC2813673

    Highly localized reflectance measurements can be used to directly quantify scatter changes in tissues. We present a microsampling approach that is used to raster scan tumors to extract parameters believed to be related to the tissue ultrastructure. A confocal reflectance imager was developed to ...
  3. Dynamic analysis of internal and external mental sweating by optical coherence tomography.

    Journal of Biomedical Optics 14(1):014026 (2009) PMID 19256714

    Mental sweating is human sweating that is accelerated via the sympathetic nerve by application of mental or physical stress. In the neurosciences, there is keen interest in this type of sweating, because the amount of sweat in response to a stress applied to a volunteer directly reflects activit...
  4. In-vivo photoacoustic microscopy of nanoshell extravasation from solid tumor vasculature.

    Journal of Biomedical Optics 14(1):010507 (2009) PMID 19256687

    In this study, high resolution backward-mode photoacoustic microscopy (PAM) is used to noninvasively image progressive extravasation and accumulation of nanoshells within a solid tumor in vivo. PAM takes advantage of the strong near-infrared absorption of nanoshells and their extravasation tende...
  5. Noninvasive in vitro and in vivo assessment of epidermal hyperkeratosis and dermal fibrosis in atopic dermatitis.

    Journal of Biomedical Optics 14(1):014008 (2009) PMID 19256696

    Atopic dermatitis (AD) is characterized by hyperkeratosis of epidermis and fibrosis within dermis in chronic skin lesions. Thus far, the histology of skin lesions has been evaluated only by examination of excised specimens. A noninvasive in vivo tool is essential to evaluate the histopathologica...
  6. Resolution in the ApoTome and the confocal laser scanning microscope: comparison.

    Journal of Biomedical Optics 14(1):014022 (2009) PMID 19256710

    The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological ...
  7. Fluorescence spectroscopy of oral tissue: Monte Carlo modeling with site-specific tissue properties.

    Journal of Biomedical Optics 14(1):014009 (2009) PMID 19256697 PMCID PMC2722954

    A Monte Carlo model with site-specific input is used to predict depth-resolved fluorescence spectra from individual normal, inflammatory, and neoplastic oral sites. Our goal in developing this model is to provide a computational tool to study how the morphological characteristics of the tissue a...
  8. Automated retinal shadow compensation of optical coherence tomography images.

    Journal of Biomedical Optics 14(1):010503 (2009) PMID 19256685

    We present an automated numerical method of compensating for retinal shadows in the choroid. In this method, signal extinction caused by retinal vessels is estimated by subtracting median A-scans obtained from beneath the retinal vessels and A-scans from the surrounding area. Adding the obtained...
  9. Optical spectroscopy for in-vitro differentiation of pediatric neoplastic and epileptogenic brain lesions.

    Journal of Biomedical Optics 14(1):014028 (2009) PMID 19256716

    The objective of this in vitro tissue study is to investigate the feasibility of using optical spectroscopy to differentiate pediatric neoplastic and epileptogenic brain from normal brain. Specimens are collected from 17 patients with brain tumors, and from 26 patients with intractable epilepsy ...
  10. Combination of different noninvasive measuring techniques: a new approach to increase accuracy of peripheral near infrared spectroscopy.

    Journal of Biomedical Optics 14(1):014014 (2009) PMID 19256702

    One of the problems of near-infrared-spectroscopy (NIRS) measurements is low reproducibility. The aim of the present study was to introduce quality criteria to increase reproducibility of peripheral NIRS measurements. In a prospective cohort study in 40 neonates, repeated NIRS measurements were ...
  11. Colloidal mesoporous silica nanoparticles with protoporphyrin IX encapsulated for photodynamic therapy.

    Journal of Biomedical Optics 14(1):014012 (2009) PMID 19256700

    Protoporphyrin IX (PpIX)-encapsulated mesoporous silica nanoparticles were synthesized, characterized, and utilized for photodynamic therapy (PDT) of cancer. Silica encapsulation is relatively transparent for activated light and can protect the PpIX against denaturation induced by the extreme bi...
  12. Forster resonance energy transfer-based total internal reflection fluorescence reader for apoptosis.

    Journal of Biomedical Optics 14(2):021003 (2009) PMID 19405716

    A fluorescence reader for the detection of Forster resonance energy transfer (FRET) on surfaces of living cells is described. The method is based on multiple total internal reflections (TIR) of an incident laser beam within a glass slide, such that individual samples on top of the glass slide ar...
  13. Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment.

    Journal of Biomedical Optics 14(2):021004 (2009) PMID 19405717

    Semiconductor quantum dots (QDs) coupled with cancer-specific targeting ligands are new promising agents for fluorescent visualization of cancer cells. Human epidermal growth factor receptor 2/neu (HER2/neu), overexpressed on the surface of many cancer cells, is an important target for cancer di...
  14. Calibration and validation of an optical sensor for intracellular oxygen measurements.

    Journal of Biomedical Optics 14(2):020506 (2009) PMID 19405711

    Calibration of fluorescent optical sensors for accurate, quantitative intracellular measurements in vivo suffers from lack of a representative medium that appropriately simulates the molecular complexity of the cytosol. We present a novel protocol for accurate intracellular oxygen sensing via fl...
  15. Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals.

    Journal of Biomedical Optics 14(2):020503 (2009) PMID 19405709

    Intrinsic two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals are shown to differentiate between normal and neoplastic human esophageal stroma. It was found that TPEF and SHG signals from normal and neoplastic stroma exhibit different organization features, provid...
  16. Extended-working-distance multiphoton micromanipulation microscope for deep-penetration imaging in live mice and tissue.

    Journal of Biomedical Optics 14(2):024032 (2009) PMID 19405761 PMCID PMC3098575

    We constructed a multiphoton (2-P) microscope with space to mount and operate microphysiology hardware, and still acquire high quality 2-P images of tumor cells deep within tissues of live mice. We reconfigured for nondescanned 2-P imaging, a dedicated electrophysiology microscope, the Nikon FN1...
  17. Delivery of optical contrast agents using Triton-X100, part 1: reversible permeabilization of live cells for intracellular labeling.

    Journal of Biomedical Optics 14(2):021012 (2009) PMID 19405725 PMCID PMC2748244

    Effective delivery of optical contrast agents into live cells remains a significant challenge. We sought to determine whether Triton-X100, a detergent commonly used for membrane isolation and protein purification, could be used to effectively and reversibly permeabilize live cells for delivery o...
  18. Quantitative diffuse reflectance and fluorescence spectroscopy: tool to monitor tumor physiology in vivo.

    Journal of Biomedical Optics 14(2):024010 (2009) PMID 19405740 PMCID PMC2724679

    This study demonstrates the use of optical spectroscopy for monitoring tumor oxygenation and metabolism in response to hyperoxic gas breathing. Hemoglobin saturation and redox ratio were quantified for a set of 14 and 9 mice, respectively, measured at baseline and during carbogen breathing (95% ...
  19. Ultrasound-modulated optical tomography in reflection mode with ring-shaped light illumination.

    Journal of Biomedical Optics 14(2):024015 (2009) PMID 19405745 PMCID PMC2716112

    We have succeeded in implementing ring-shaped light illumination ultrasound-modulated optical tomography (UOT) in reflection mode. The system used intense acoustic bursts and a charge-coupled device (CCD) camera-based speckle contrast detection method. In addition, the implementation allows plac...
  20. Photoacoustic molecular imaging with antibody-functionalized single-walled carbon nanotubes for early diagnosis of tumor.

    Journal of Biomedical Optics 14(2):021008 (2009) PMID 19405721

    Single-walled carbon nanotubes (SWNT) in a poly(ethylene)ghycol solution are a biocompatible transporters with strong optical absorption in the near-infrared region, in which the biological tissue is almost transparent with very low absorbance. Here, antibody-functionalized SWNTs for tumor early...