Fungal Genetics and Biology
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From the Guest Editors
Expressed sequence tags and genes associated with loline alkaloid expression by the fungal endophyte Neotyphodium uncinatum
Loline alkaloids (LA), which are 1-aminopyrrolizidines with an oxygen bridge, are produced by Epichloe¨ (anamorph=Neotyphodium) species, endophytes of grasses. LA are insecticidal, thus, helping to protect host plants from insect herbivory. The objective of this study was to identify genes associate...
A high-affinity ammonium transporter from the mycorrhizal ascomycete Tuber borchii
An ammonium transporter cDNA, named TbAMT1, was isolated from the ectomycorrhizal ascomycetous truffle Tuber borchii. The polypeptide encoded by TbAMT1 (52kDa) functionally complements ammonium uptake-defective yeast mutants and shares sequence similarity with previously characterized ammonium transp...
Light-mediated participation of the VIVID-like protein of Fusarium fujikuroi VvdA in pigmentation and development.
We investigate the role of other blue-light photoreceptors. Here we describe the identification, regulation and targeted mutation of the F. fujikuroi gene vvdA, homologous of the Neurospora crassa vivid (vvd) gene. As found for vvd in N. crassa, expression of vvdA in F. fujikuroi is strongly stimula...
The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus pos...
I to VII previously established, and support the grouping of these into three divisions. Disruption of the gene coding PdChsVII, which contains a short version of a myosin motor domain, has been achieved by using Agrobacterium tumefaciens-mediated transformation and revealed its role in the life cyc...
Natural occurrence of mycotoxins in medicinal plants: a review.
Medicinal plants are widely used as home remedies and raw materials for the pharmaceutical industries. Herbal remedies are used in the prevention, treatment and cure of disorders and diseases since ancient times. However, use of medicinal herbs may not meet the requirements of quality, safety and ef...
Categorisation of sugar acid dehydratases in Aspergillus niger.
In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins wi...
Adenine deaminase is encoded by Tad1 and participates in copper accumulation in Trichoderma reesei.
We cloned a novel Tad1 gene and demonstrated that this gene is closely involved in copper bioaccumulation in Trichoderma reesei. Tad1 gene encodes a 510 amino acids protein of the amidohydrolase superfamily which belongs to COG0402. We found that adenine was the most efficient substrate of Tad1 prot...
Spliceosome twin introns in fungal nuclear transcripts.
We have here predicted analogous instances of spliceosomal twintrons ("stwintrons") in filamentous fungi. In two specific cases, where the internal intron interrupts the donor of the external intron after the first or after the second nucleotide, respectively, we show that intermediates with the seq...