The International Journal of Biochemistry & Cell Biology
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- Sciencedirect from 1995, Rcgp, Gale, CSA, Proquest, and Ingenta
FGFR signalling in women's cancers.
We outline the molecular mechanisms of FGFR signalling and discuss the role of this pathway in women's cancers, focusing on breast, endometrial, ovarian and cervical carcinomas, and their associated preclinical and clinical data. We also address the rationale for therapeutic intervention and the nee...
Enhanced mesenchymal stem cell survival induced by GATA-4 overexpression is partially mediated by regulation of the miR-15 family.
We reported previously that pre-programming mesenchymal stem cells with the GATA-4 gene increases significantly cell survival in an ischemic environment. In this study, we tested whether regulation of microRNAs and their target proteins was associated with the cytoprotective effects of GATA-4. Mesen...
miR-320a regulates erythroid differentiation through MAR binding protein SMAR1.
We found that miR-320a inhibits erythroid differentiation by targeting Matrix Attachment Region binding protein SMAR1. miR-320a negatively regulates the expression of SMAR1 by directly binding to its 3'UTR. In response to mild DNA damage, miR-320a expression is decreased resulting in enhanced expres...
Molecular mechanisms of muscle atrophy in myotonic dystrophies.
Myotonic dystrophy type 1 (DM1) and myotonic dystrophy type 2 (DM2) are multisystemic diseases that primarily affect skeletal muscle, causing myotonia, muscle atrophy, and muscle weakness. DM1 and DM2 pathologies are caused by expansion of CTG and CCTG repeats in non-coding regions of the genes enco...
Identification of DAPK as a scaffold protein for the LIMK/cofilin complex in TNF-induced apoptosis.
We performed a peptide array screen. We show that TNF-treatment enhanced the phosphorylation of LIMK at threonine508 and its downstream target cofilin at serine3 (p-cofilin(Ser3)). Modulation of DAPK activity and expression by DAPK inhibitor treatment, siRNA knockdown, and overexpression affected th...
A novel retro-inverso peptide is a preferential JNK substrate-competitive inhibitor.
We observed a 5-fold increase in the potency of the retro-inverso form, D-PYC98 (a d-amino acid peptide in the reversed sequence) when compared with the inhibition achieved by L-PYC98, prompting our further evaluation of the D-PYC98 inhibitory mechanism. In vitro assays revealed that, in addition to...
Dysferlin interacts with calsequestrin-1, myomesin-2 and dynein in human skeletal muscle.
Our objective was to unravel the proteins that constitute the dysferlin complex and their interaction within the complex using immunoprecipitation assays (IP), blue native gel electrophoresis (BN) in healthy adult skeletal muscle and healthy cultured myotubes, and fluorescence lifetime imaging-fluor...
Erythropoietin contributes to slow oxidative muscle fiber specification via PGC-1α and AMPK activation.
We examined the effect of erythropoietin signaling on skeletal muscle fiber type development. Skeletal muscles that are rich in slow twitch fibers are associated with increased mitochondrial oxidative activity and corresponding expression of related genes compared to muscle rich in fast twitch fiber...
Leukocyte integrins αLβ2, αMβ2 and αXβ2 as collagen receptors-Receptor activation and recognition of GFOGER motif.
We produced the corresponding integrin αI domains both in wild-type and activated form and measured their binding to collagens I-VI. In the "closed" (wild-type) conformation, the αLI and αMI domains bound with low avidity to their primary ligands, and the interaction with collagens was also very wea...
Destabilization of CDC6 upon DNA damage is dependent on neddylation but independent of Cullin E3 ligases.
We studied whether Cullin RING E3 ligases also play a role in the turnover of CDC6 protein in mammalian cells. To this end, we used the Nedd8 E1 inhibitor MLN4924, which blocks the activity of all Cullin E3 ligases. We observed that treatment with MLN4924 increased CDC6 protein expression. However,...