Archaea 3,417 articles
Intramolecular stable carbon isotopic analysis of archaeal glycosyl tetraether lipids.
We developed a procedure to analyze the δ(13)C values of glycosidic headgroups. Successful determination was achieved by (1) monitoring the contamination from free sugars during lipid extraction and preparation, (2) optimizing the hydrolytic conditions for glycolipids, and (3) derivatizing the resu...
A Major Clade of Prokaryotes with Ancient Adaptations to Life on Land
We show how combined evidence from phylogenetic, cytological, and environmental data support the existence of an evolutionary group that appears to have had a common ancestor on land early in Earth's history and includes two-thirds of known prokaryote species. Members of this terrestrial clade (Terr...
[Determination of diversity peat soil diazotrophic microorganisms by cloning nifH gene].
Characterization of a Bacillus subtilis 64-kDa DNA Polymerase X Potentially Involved in DNA Repair
Bacillus subtilis gene yshC encodes a 64-kDa family X DNA polymerase (PolXBs), which contains all the critical residues involved in DNA and nucleotide binding as well as those responsible for catalysis of DNA polymerization, conserved in most family X members. Biochemical analyses of the purified en...
Identification of Archaeal population in the granular sludge of an UASB reactor treating sewage at low temperatures.
Effect of low temperature on up-flow anaerobic sludge bed (UASB) reactor performance treating raw sewage was investigated in terms of the variations in methanogenic diversity using the 16S rRNA based Fluorescence In-Situ Hybridization (FISH) technique. The diversity of microorganisms present in the...
An alternative menaquinone biosynthetic pathway operating in microorganisms.
We deduced the outline of this alternative pathway in a nonpathogenic strain of Streptomyces by bioinformatic screening, gene knockouts, shotgun cloning with isolated mutants, and in vitro studies with recombinant enzymes. As humans and commensal intestinal bacteria, including lactobacilli, lack thi...
Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor
A total community DNA sample from an agricultural biogas reactor continuously fed with maize silage, green rye, and small proportions of chicken manure has recently been sequenced using massively parallel pyrosequencing. In this study, the sample was computationally characterized without a prior ass...
Phylogenetic analyses of archaeal ribosomal DNA sequences from salt pan sediment of Mumbai, India.
The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two uniqu...
P"I"I signal transducers: novel functional and structural insights
The P(II) signal transduction proteins have key functions in coordinating the regulation of central metabolic processes. Signals from the carbon, nitrogen and energy status of the cells are converted into different conformational (and modification) states of the P(II) proteins. Depending on these st...
Evidence for a gram-positive, eubacterial root of the tree of life.
We apply a newly developed rooting algorithm, top-down rooting, to indels found in informational and operational gene sets, introduce new computational tools for indel analyses, and present evidence (P < .01) that the root of the tree of life is not present in its traditional location, between the E...