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All Diagnosis Diagnostic Techniques and Procedures Diagnostic Imaging Microscopy Microscopy, Fluorescence

Microscopy, Fluorescence 4,428 articles

  1. Single-molecule imaging of nuclear transport.

    Journal of Visualized Experiments (40) (2010) PMID 20548283

    We described a protocol for the methodological approach with an improved spatiotemporal resolution of 0.4 ms and 12 nm. The improved resolution enabled us to capture transient active transport and passive diffusion events through the nuclear pore complexes (NPC) in semi-intact cells. We expect this...
  2. Fluorescent Labeling of Drosophila Heart Structures

    Journal of Visualized Experiments (32) (2009) PMID 19826399

  3. Calcium imaging of cortical neurons using Fura-2 AM.

    Journal of Visualized Experiments (23) (2009) PMID 19229178

  4. Tracking dynamics of muscle engraftment in small animals by in vivo fluorescent imaging.

    Journal of Visualized Experiments (31) (2009) PMID 19770816

    We demonstrate that in vivo fluorescence imaging is a sensitive and reliable method for tracking transplanted GFP (Green Fluorescent Protein)-labeled cells in mouse skeletal muscles. Despite the concern about background due to the use of an external light necessary for excitation of fluorescent prot...
  5. APUD-like cells in the primitive gut of a 27 day old human embryo.

    Archives d'anatomie microscopique et de morphol... 75(2):111 (1986) PMID 2881515

    We could observe that a few cells exhibiting greenish fluorescence were located in the epithelium wall of the fore gut. Most of them were situated in contact with the basal lamina and possessed a long apical process extending over towards the gut lumen. The uptake of amine precursor, the morphology...
  6. Labeling stem cells with fluorescent dyes for non-invasive detection with optical imaging.

    Journal of Visualized Experiments (14) (2008) PMID 19066580

    We label human mesenchymal stem cells (hMSC) by simple incubation with the lipophilic fluorescent dye DiD (C67H103CIN2O3S) and how we label human embryonic stem cells (hESC) with the FDA approved fluorescent dye Indocyanine Green (ICG). The uptake mechanism is via adherence and diffusion of the lypo...
  7. Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis.

    Cancer and Metastasis Reviews 17(3):271 (1998) PMID 10352880

    We took advantage of the green fluorescent protein (GFP) of the jellyfish, Aequorea victoria. A series of cancer cell lines have been stably transfected with vectors containing humanized GFP cDNA. To utilize GFP expression for metastasis studies, fragments of subcutaneously growing tumor, which were...
  8. Morphological studies of effector cell-microvessel interactions in adoptive immunotherapy in tumor-bearing animals.

    Natural Immunity 15(2-3):78 (1996) PMID 9162266

    We have investigated the migration from the vasculature towards malignant tissues of various effector cells. Our results indicate that murine A-NK cells seem to be arrested for an extended period of time in the microvasculature and also, although infrequently, to adhere to the endothelial lining of...
  9. [Regressive changes in the placenta in early pregnancy in women with primary arterial hypertension].

    Roczniki Akademii Medycznej w Bialymstoku = Ann... 35-36:71 (1990) PMID 1726680

    Morphological methods, in particular histochemical technique of secondary fluorescence according to Bertalanffy were used for the assessment of regressive changes of placenta. In women with primary arterial hypertension during early pregnancy fibrinoid masses in decidua, chorion, anchoring willi, vi...
  10. High-content single-cell drug screening with phosphospecific flow cytometry.

    Orthopedics 31(10) (2008) PMID 19225997

    We describe a high-content, cell-based drug discovery platform based on phosphospecific flow cytometry, or phosphoflow, that enabled screening for inhibitors against multiple endogenous kinase signaling pathways in heterogeneous primary cell populations at the single-cell level. From a library of sm...