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All Diagnosis Diagnostic Techniques and Procedures Diagnostic Imaging Microscopy Microscopy, Fluorescence

Microscopy, Fluorescence 58 articles

  1. Time-lapse imaging of mitosis after siRNA transfection.

    Journal of Visualized Experiments (40) (2010) PMID 20548280

    We present a basic method for assessing the effect of depleting a potential mitotic regulatory protein on the timing of mitosis. Cells are transfected with siRNA, placed in a stage-top incubation chamber, and imaged using an automated fluorescence microscope. We describe how to use software to set u...
  2. Antifouling self-assembled monolayers on microelectrodes for patterning biomolecules.

    Journal of Visualized Experiments (30) (2009) PMID 19707178

    We present a procedure for forming a poly(ethylene glycol) (PEG) trimethoxysilane self-assembled monolayer (SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single assembly step and prevents biofouling on silicon and gold surfaces. The SAM is used to coat microelectr...
  3. Single-molecule imaging of nuclear transport.

    Journal of Visualized Experiments (40) (2010) PMID 20548283

    We described a protocol for the methodological approach with an improved spatiotemporal resolution of 0.4 ms and 12 nm. The improved resolution enabled us to capture transient active transport and passive diffusion events through the nuclear pore complexes (NPC) in semi-intact cells. We expect this...
  4. High-content single-cell drug screening with phosphospecific flow cytometry.

    Orthopedics 31(10) (2008) PMID 19225997

    We describe a high-content, cell-based drug discovery platform based on phosphospecific flow cytometry, or phosphoflow, that enabled screening for inhibitors against multiple endogenous kinase signaling pathways in heterogeneous primary cell populations at the single-cell level. From a library of sm...

    Acta Microbiologica Academiae Scientiarum Hunga... 11:357 (1964) PMID 14314672

  6. Live cell imaging of F-actin dynamics via Fluorescent Speckle Microscopy (FSM).

    Journal of Visualized Experiments (30) (2009) PMID 19684563

    We describe the use of Fluorescent Speckle Microscopy (FSM) to capture high-resolution images of actin dynamics in PtK1 cells. A unique advantage of FSM is its ability to capture the movement and turnover kinetics (assembly/disassembly) of the F-actin network within living cells. This technique is p...
  7. A simple method for the direct visualization of cell surface-associated lectins and antibodies in freeze fracture electron microscopy. FAU -...

    Ultramicroscopy 12(3):253 (1983) PMID 6372188

  8. Using the gene pulser MXcell electroporation system to transfect primary cells with high efficiency.

    Journal of Visualized Experiments (35) (2010) PMID 20057352

    We will demonstrate how to perform a simple experiment to quickly identify the best electroporation conditions. We will demonstrate how to run several samples through a range of electroporation conditions so that an experiment can be conducted at the same time as optimization is performed. We will a...
  9. Cryostat technique for central nervous system histofluorescence.

    Histochemie. Histochemistry. Histochimie 50(2):119 (1976) PMID 1053221

    This paper offers a technique for obtaining monoamine histofluorescence in the CNS by means of formaldehyde perfusion followed by cryostat sectioning. No freeze-drying is involved. Cryostat sections are exposed to formaldehyde vapor to complete the fluorophore formation. The fluorescence thus obtain...
  10. Enzyme-synthesized poly(amine-co-esters) as nonviral vectors for gene delivery.

    Journal of Biomedical Materials Research, Part A 96(2):456 (2011) PMID 21171165

    A family of biodegradable poly(amine-co-esters) was synthesized in one step via enzymatic copolymerization of diesters with amino-substituted diols. Diesters of length C(4) -C(12) (i.e., from succinate to dodecanedioate) were successfully copolymerized with diethanolamines with eithe...