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All Diagnosis Diagnostic Techniques and Procedures Diagnostic Imaging Microscopy Microscopy, Fluorescence

Microscopy, Fluorescence 4,468 articles


  1. Fluid streaming above interdigitated electrodes in dielectrophoresis experiments.

    Electrophoresis 32(18):2448 (2011) PMID 21922492

    For the investigation of alternating current electrokinetic effects, a system is presented that allows for the simultaneous observation of fluid flow above and around microelectrodes in all three directions in space. Beside the usual microscopical view from top, lateral observation t...
  2. Tissue-engineered constructs based on SPCL scaffolds cultured with goat marrow cells: functionality in femoral defects.

    Journal Of Tissue Engineering And Regenerative ... 5(1):41 (2011) PMID 20603869

    This study aims to assess the in vivo performance of cell-scaffold constructs composed of goat marrow stromal cells (GBMCs) and SPCL (a blend of starch with polycaprolactone) fibre mesh scaffolds at different stages of development, using an autologous model. GBMCs from iliac crests w...
  3. pH-Sensitive, N-ethoxybenzylimidazole (NEBI) bifunctional crosslinkers enable triggered release of therapeutics from drug delivery carriers.

    Organic & Biomolecular Chemistry 8(22):5105 (2010) PMID 20820656

    We demonstrate that an analog of doxorubicin (a representative example of an anticancer therapeutic) conjugated to human serum albumin (HSA, a representative example of a macromolecular carrier) via this NEBI crosslinker can internalize and localize into acidic lysosomes of ovarian cancer cells. Flu...
  4. Enhanced fluorescence of curcumin on plasmonic platforms.

    Current Pharmaceutical Biotechnology 11(2):223 (2010) PMID 20210735

    We report a significant increase of a curcumin (1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) fluorescence brightness when deposited on plasmonic platforms (self assembled silver nanostructures formed on the surface of silver semitransparent film). The enhancement of fluorescence inte...
  5. Single-molecule imaging of nuclear transport.

    Journal of Visualized Experiments (40) (2010) PMID 20548283

    We described a protocol for the methodological approach with an improved spatiotemporal resolution of 0.4 ms and 12 nm. The improved resolution enabled us to capture transient active transport and passive diffusion events through the nuclear pore complexes (NPC) in semi-intact cells. We expect this...
  6. Microinjection of Helobdella (leech) embryos.

    Cold Spring Harbor Protocols 2009(4):pdb.prot5190 (2009) PMID 20147133

    One advantage of using Helobdella (leech) embryos as an experimental system is their amenability for microinjection. Blastomeres ranging in size from the zygote (400 microm diameter) down to micromeres and primary blast cells (approximately 20 microm diameter) can be injected by pres...
  7. Development of a highly specific rhodamine-based fluorescence probe for hypochlorous acid and its application to real-time imaging of phagoc...

    Journal of the American Chemical Society 129(23):7313 (2007) PMID 17506554

    We have developed new TMR derivatives that show different dependences of their behavior upon the environment. Among them, HMTMR showed unique characteristics, and its putative spirocyclic structure was confirmed by X-ray crystallography. Utilizing this discovery, we have established a strategy to mo...
  8. [Transfection with 5-lipoxygenase/green fluorescence protein for evaluating injury-induced 5-lipoxygenase translocation to the nuclear membr...

    Zhejiang da xue xue bao. Yi xue ban = Journal o... 36(2):111 (2007) PMID 17443895

    OBJECTIVE: To evaluate the translocation of 5-lipoxygenase (5-LOX)) after injuries by transfection with green fluorescence protein (GFP)/5-LOX in PC12 cells. METHODS: PC12 cells were stably transfected with pEGFP-C2/5-LOX (GFP/5-LOX) or pEGFP-C2 vectors (control). After treatment with oxygen-glucose...
  9. The Mre11 Complex Influences DNA Repair, Synapsis, and Crossing Over in Murine Meiosis

    Current Biology 17(4):6 (2007) PMID 17291760 PMCID 1839861

    We analyzed meiotic events in mice harboring hypomorphic Mre11 and Nbs1 mutations which, unlike null mutants, support viability . Our studies revealed defects in the temporal progression of meiotic prophase, incomplete and aberrant synapsis of homologous chromosomes, persistence of strand exchange p...
  10. Subcellular trafficking of the yeast plasma membrane ABC transporter, Pdr5, is impaired by a mutation in the N-terminal nucleotide-binding f...

    Molecular Microbiology 63(3):811 (2007) PMID 17302805

    We have isolated several mutated forms that fail to confer resistance to cycloheximide and itraconazole. Here, we examined two variants, the expression of which was abnormally low when cells reach the stationary phase of growth. The Pdr5(1157) variant lacked the C-terminal transmembrane domain due t...