Saccharomycopsis 15 articles
Structure-function relationships in the catalytic and starch binding domains of glucoamylase.
Sixteen primary sequences from five sub-families of fungal, yeast and bacterial glucoamylases were related to structural information from the model of the catalytic domain of Aspergillus awamori var. X100 glucoamylase obtained by protein crystallography. This domain is composed of th...
Characterization of ATPase activity in the mycelial form cells of yeast Saccharomycopsis fibuligera.
1. The properties of ATPase activity were studied with the mycelial form cells of Saccharomycopsis fibuligera. 2. Optimal pH for the activity was about 9.5. 3. The activity was stimulated by Mg2+. 4. The activity was inhibited by DCCD, NaF and oligomycin, but not inhibited by ouabain.
Genetic control of lysine permeases in Saccharomycopsis lipolytica.
In order to obtain strains of Saccharomycopsis lipolytica impaired in the active transport of L-lysine, mutants resistant to a mixture of L-canavanine, L-4-5-transdehydrolysine and L-S-amino ethylcysteine, taken either all three or two by two, were isolated. These compounds were shown previously to...
[Biodegradation of a lipid-rich effluent by native bacteria].
From samples of effluent derived from a biological treatment plant of a mayonnaise and margarine producing factory, several bacteria strains presenting high lipolytic activities were isolated. The strain having the highest activity was used for treating a typical final effluent and the results obtai...
Trehalose accumulation in a high-trehalose-accumulating mutant of Saccharomycopsis fibuligera sdu does not respond to stress treatments.
The isolation of high-trehalose-accumulating mutant A11 from Saccharomycopsis fibuligera sdu has been previously described. In this paper, accumulation of trehalose under various stress conditions in S. fibuligera A11 was investigated. Neither activation of trehalose-6-phosphate synthase (SfTps1) no...
Genetic and enzymatic characterisation of glucoamylases from yeast.
The nucleotide sequence of the glucoamylase gene GLA1 from Saccharomycopsis fibuligera KZ.
The nucleotide sequence of the 2544-bp PstI fragment carrying the glucoamylase gene of Saccharomycopsis fibuligera KZ, designated as GLA1, has been determined. When compared with the nucleotide sequence of the GLU1 gene  one nucleotide substitution was found in the 321- bp of the 5'-flanking regio...
Use of nystatin for random spore selection in the yeast Saccharomycopsis lipolytica.
Nystatin was used to develop a new method to select spores of the yeast Saccharomycopsis lipolytica. At low concentrations nystatin killed preferently growing cells of this yeast. At high concentrations nongrowing cells were affected as well. In contrast, spores were not sensitive to nystatin action...
Isolation and characterization of a unique endo-beta-1,6-glucanase from the yeast Saccharomycopsis fibuligera NCYC 451.
A beta-glucanase enzyme has been described which has beta-1,6 activity but no beta-1,3 activity. It was isolated and purified from cell free extract and culture free medium of Saccharomycopsis fibuligera by a combination of techniques that included adsorption on DEAE-Sepharose and gel filtration thr...
Modulation of biorecognition of glucoamylases with Concanavalin A by glycosylation via recombinant expression
Various types of glucoamylases were prepared to modulate their biospecific interaction with Concanavalin A. Glucoamylase Glm was isolated from the native yeast strain Saccharomycopsis fibuligera IFO 0111. Two glycosylated recombinant glucoamylases Glu's of S. fibuligera HUT 7212 were expressed and i...