Abstract
We performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compou...
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PMID: 21612302
PDF is available here.
Abstract
Efficient nutrient removal in decentralized wastewater treatment systems is a challenging task. To improve the removal of organic matter and nitrogen from wastewater, two types of bioreactors using membrane-aerated biofilm reactor (MABR) and microbial fuel cell (MFC) techniques were evaluated. During...
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PMID: 21905409
PDF is available here.
Abstract
Our results confirm the importance of pH and related geochemical factors in fine-tuning acidophilic microbial community structure and function at the species and strain level, and demonstrate the broad utility of proteomics in laboratory community studies....
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PMID: 21228889
PDF is available here.
Abstract
Deep-seated mycosis usually occurred in severe immunocompromised patients and is sometimes fatal. Hence, chronic fungal infection occurred in the patients with mild to moderate immunocompromised status and persists for longer period. Biofilm formation is one of the factors related to persisting infec...
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PMID: 21861435
PDF is available here.
Abstract
Central venous catheters (CVCs) nowadays constitute critical devices used in medical care, namely in intensive care units. However, CVCs also represent one of the indwelling medical devices with enhanced risk of nosocomial device-related infection. Catheter-related infections (CRIs) are a major cause...
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PMID: 21718230
PDF is available here.
Abstract
Innovative wastewater treatment technologies are developed to respond to changing regulatory requirements, increase efficiency, and enhance sustainability or to reduce capital or operating costs. Drawing from experience of five successful new process introductions from both the inventor/developer's a...
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PMID: 21751707
PDF is available here.
Abstract
Herpesviruses, including Epstein-Barr virus and cytomegalovirus, occur at high copy counts in aggressive periodontitis, and may interact synergistically with periodontopathic bacteria in the etiology of the disease. Herpesvirus active periodontal infections may impair local host defenses and thus inc...
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PMID: 21823497
PDF is available here.
Abstract
It has been well established that dental caries results from the accumulation of dental plaque on tooth surfaces. Several decades of in vitro and as well as clinical studies have identified Streptococcus mutans as an important etiological agent in carious lesion formation. In additio...
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PMID: 21874934
PDF is available here.
Abstract
Household water in Oman, as well as in other countries in the region, is stored in tanks placed on house roofs that can be subjected to physicochemical factors which can promote microbial growth, including pathogens and opportunistic pathogens which pose health risks. Water samples were collected fro...
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PMID: 21942202
PDF is available here.
Abstract
Echinocandins represent the newest class of antifungal agents. Currently, three echinocandins, anidulafungin, caspofungin and micafungin are licensed for clinical use in various indications. They act as inhibitors of β-(1,3)-glucan synthesis in the fungal cell wall and have a favorable pharmacologic...
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PMID: 21486730
PDF is available here.
Abstract
I use a model system of stream biofilms to show that niche partitioning among species of algae can increase the uptake and storage of nitrate, a nutrient pollutant of global concern. I manipulated the number of species of algae growing in the biofilms of 150 stream mesocosms that had been set up to...
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PMID: 21475199
PDF is available here.
Abstract
We compared the exoproteome of a nasal carrier strain of SA to a genetically similar noncarrier strain. Additionally, the carrier strain biofilm exoproteome was also compared against its planktonic counterpart. Using high throughput proteomics, it was observed that the carrier strain of SA secretes...
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PMID: 21338050
PDF is available here.
Abstract
It aims to investigate the changes in composition and structure of bacterial communities de-veloping on biological activated carbon (BAC) particles, and the bacterial functions.
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PMID: 21565683
PDF is available here.
Abstract
Some microfungi are known to be opportunistic human pathogens, and there is a body of scientific opinion that one of their routes of infection may be water aerosols. Others have been implicated as causative agents of odours and off-tastes in drinking water. This study was undertaken...
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PMID: 21556175
PDF is available here.
Abstract
To review the evidence for the presence of bacterial biofilms in chronic rhinosinusitis (CRS) and mechanisms by which they may contribute to the chronic inflammation characteristic of this disease. Lastly, to provide an overview of the current and potential future treatments for bact...
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PMID: 21150431
PDF is available here.
Abstract
We describe here the role of muramidases present in clones of metagenomic DNA that result in cell aggregation and biofilm formation by Escherichia coli. The metagenomic clones were obtained from uncultured Lachnospiraceae-affiliated bacteria resident in the foregut microbiome of the Tammar wallaby....
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PMID: 20668486
PDF is available here.
Abstract
Cell surface physicochemical characterization techniques were combined with quantitative changes in protein expression, to investigate the biological and biophysical changes of Escherichia coli MG1655 cells when grown as a biofilm (BIO). The overall surface charge of BIO cells was fo...
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PMID: 21268264
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Abstract
We report how LapD differentially controls maintenance and release of LapA: c-di-GMP binding to LapD promotes interaction with and inhibition of the periplasmic protease LapG, which targets the N-terminus of LapA. We identify conserved amino acids in LapA required for cleavage by LapG. Mutating thes...
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PMID: 21304920
PDF is available here.
Abstract
We elucidate the molecular mechanism of autoinhibition and activation of LapD based on structure-function analyses and crystal structures of the entire periplasmic domain and the intracellular signaling unit in two different states. In the absence of c-di-GMP, the intracellular module assumes an ina...
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PMID: 21304926
PDF is available here.
Abstract
We have shown previously that spontaneous lateral transfer of non-conjugative plasmids occurs in a colony biofilm of mixed E. coli strains (a set of a donor strain harbouring a plasmid and a plasmid-free recipient strain). In this study, with high-frequency combinations of strains and a plasmid, we...
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PMID: 21283723
PDF is available here.
Abstract
To better reveal the functions of key members involved in cyclic di-GMP signal metabolism pathways in the bacterial blight pathogen of rice Xanthomonas oryzae pv. oryzae (Xoo).
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PMID: 21465786
PDF is available here.
Abstract
We present a method for the extraction and quantitation of the P. aeruginosa QS signal N-3-oxo-dodecanoyl-homoserine lactone, and its rearranged tetramic acid product, C12-TA, which itself has implications as a survival tactic used by P. aeruginosa....
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PMID: 21031307
PDF is available here.
Abstract
We describe the usage of reporter strains consisting of a lasB-gfp or rhlA-gfp fusion in P. aeruginosa for qualitative and quantitative evaluation of the inhibition of the two major QS pathways, monitored as reduced expression of green fluorescence. By the use of an in vitro flow cell system it is p...
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PMID: 21031317
PDF is available here.
Abstract
We describe several methods for the analysis of the effects of farnesol on biofilm formation and function....
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PMID: 21031315
PDF is available here.
Abstract
A hybrid activated sludge/biofilm process was investigated for wastewater treatment in a cold climate region. This process, which contains both suspended biomass and biofilm, usually referred as IFAS process, is created by introducing plastic elements as biofilm carrier media into a conventional acti...
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PMID: 21436546
PDF is available here.
Abstract
We investigated the phylogenetic distribution of the bacteria present in an operating rainwater tank by denaturing gradient gel electrophoresis (DGGE), and compared the bacterial composition in rainwater and biofilm from the inlet and outlet of the tank. Seventeen species were identified, the DGGE p...
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PMID: 21508560
PDF is available here.
Abstract
We present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the co...
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PMID: 20938507
PDF is available here.
Abstract
These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface....
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PMID: 21040512
PDF is available here.
Abstract
To explore the relationship between the bacterial biofilm, allergy, inflammatory cytokines and refractory rhinosinusitis.
According to the assessment of symptom by visual analogue scale and nasal endoscopy by Lund-Kennedy system, 19 patients with persistent chronic rh...
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PMID: 21215049
PDF is available here.
Abstract
We grew S. aureus in serum and analyzed biofilm thickness and expression of biofilm-related genes. Whereas serum supported planktonic bacterial growth, it was a potent inhibitor of biofilm formation. The inhibitory serum component had a molecular weight less than 3000 kDa. The component was proteas...
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PMID: 20673798
PDF is available here.
Abstract
We investigated if some NSAIDs increment or diminish the secretion of aspartyl-proteases (Sap) by Candida albicans grown under different phenotypes and oxygen availability using a set of SAP knock-out mutants and other set for genes (EFG1 and CPH1) that codify transcription factors involved in filam...
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PMID: 20708674
PDF is available here.
Abstract
We determined that posttranslational modification and expression of amino-acid sequence variants change as a function of biofilm maturation. For Cytochrome₅₇₉ (Cyt₅₇₉), the most abundant cytochrome in the biofilms, late developmental-stage biofilms differed from early-stage biofilms in N...
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PMID: 20485387
PDF is available here.
Abstract
Bacterial biofilms have been observed in many patients with chronic rhinosinusitis, but their importance is still being investigated. This study examines the association between biofilms and other clinical findings in chronic rhinosinusitis patients. Twenty-four patients with chronic rhinosinusitis...
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PMID: 20974342
PDF is available here.
Abstract
The goal of this comparative study was to investigate biofilm forming microorganisms living in washing machines (WMs). Biofilms were sampled from 11 washing machines from four countries and three continents. Among the 94 isolated strains, 30% were potential human pathogens. Representative strains we...
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PMID: 20954022
PDF is available here.
Abstract
The effective removal of nitrogen compounds from wastewater has become a critical issue for treatment plants as the awareness of their negative impact on the environment increased. Autotrophic nitrogen removal has become an interesting alternative to the more conventional heterotrophic processes, as...
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PMID: 21121462
PDF is available here.
Abstract
Altogether 118 Pseudomonas aeruginosa strains isolated from urine of patients with urinary tract infection were tested by the disk diffusion method for susceptibility to ciprofloxacin, ofloxacin, gentamicin, amikacin, colistin, meropenem, imipenem, piperacillin/tazobactam and ceftazidime. All strain...
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PMID: 21114003
PDF is available here.
Abstract
Lipopeptides constitute a structurally diverse group of metabolites produced by various bacterial and fungal genera. In the past decades, research on lipopeptides has been fueled by their antimicrobial, antitumour, immunosuppressant and surfactant activities. However, the natural functions of lipope...
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PMID: 20412310
PDF is available here.
Abstract
We developed a new medium (SHI medium) by combining the ingredients of several selected media that can support different subpopulations within the original oral microbial community derived from pooled saliva. DGGE and 454 pyrosequencing analysis showed that SHI medium was capable of supporting a mor...
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PMID: 20883224
PDF is available here.
Abstract
Oral biofilm-related diseases such as periodontal and peri-implant diseases are unique infections in that they develop from the resident indigenous microflora. As more implants are nowadays being placed, clinicians may encounter more complications. Therefore, understanding the etiology is warranted...
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PMID: 20881809
PDF is available here.
Abstract
We compared cytokine induction in human monocytes and mouse macrophages by planktonic and biofilm bacteria. We compared Pseudomonas aeruginosa and Staphylococcus aureus, two bacteria often colonizing the airways of cystic fibrosis patients. Planktonic and biofilm S. aureus induced equivalent amounts...
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PMID: 19710099
PDF is available here.
Abstract
Bacterial attachment and growth on material surfaces are considered to be the primary steps leading to the formation of biofilm. Biofilms in hospital and food processing settings can result in bacterial infection and food contamination, respectively. Prevention of bacterial attachment, therefore, is...
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PMID: 20938849
PDF is available here.
Abstract
We identified the culturable bacteria forming a biofilm in the microalgal cells by 16S rDNA sequencing. At least eight different culturable species of bacteria were detected in the biofilm and were evaluated for the presence of quorum-sensing signals in these bacteria. Few studies have considered th...
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PMID: 20502890
PDF is available here.
Abstract
Root-associating bacteria of the nipa palm (Nypa fruticans), preferring brackish-water affected mud in Sarawak, Malaysia, were investigated. In a comparison of rhizobacterial microbiota between the nipa and the sago (Metroxylon sagu) palm, it was found that the nipa palm possessed a group of Burkhol...
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PMID: 20834139
PDF is available here.
Abstract
To evaluate virulence factors involved in the adhesion process, such as cell surface hydrophobicity (CSH), adherence to plastic capacity, adherence capacity to buccal epithelial cells (BEC), and biofilm formation, in 17 strains of C. albicans isolated from bronchial aspirates of critically ill patien...
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PMID: 20849975
PDF is available here.
Abstract
We report the formation of biofilm streamers suspended in the middle plane of curved microchannels under conditions of laminar flow. Experiments with different mutant strains allow us to identify a link between the accumulation of extracellular matrix and the development of these structures. Numeric...
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PMID: 20356880
PDF is available here.
Abstract
We demonstrate bacterial ordering and oriented attachment on the single-cell level induced by nanometer-scale periodic surface features. These surfaces cause spontaneous and distinct patterning phases, depending on their periodicity, which is observed for several strains, both gram positive and nega...
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PMID: 20687595
PDF is available here.
Abstract
This report is based on a Hygienist Panel Meeting held at St Anne's Manor, Wokingham on 24-25 June 2009. The panel agreed that greater use should be made of antiseptics to reduce reliance on antibiotics with their associated risk of antibiotic resistance. When choosing an antiseptic for clinical use...
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PMID: 20819330
PDF is available here.
Abstract
We sought to determine the distribution of ACME in 128 S. epidermidis blood culture isolates from neonates and to assess ACME's impact on antibiotic resistance, biofilm production, invasive capacity, and host inflammatory response. ACME was detected in 15/64 (23%) invasive blood culture isolates and...
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PMID: 20703143
PDF is available here.
Abstract
We show that bacteria are also capable of olfaction. Bacillus licheniformis was able to sense airborne volatile metabolites produced by neighbouring bacterial cultures and cells could respond to this chemical information in a coordinated way. When Bacillus licheniformis was grown in a microtitre pla...
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PMID: 20721987
PDF is available here.
Abstract
Magnetic resonance microscopy (MRM) was successfully applied for non-invasive online monitoring of biofilm development, sloughing, and forced detachment. Biofilm cultivation was performed in a tube reactor directly placed in the MRM scanner. Based on the differences in relaxation time of free and bo...
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PMID: 20506514
PDF is available here.
Abstract
Industrial waste streams may contain contaminants that are valuable like Pd(II) and/or toxic and mutagenic like Cr(VI). Using Serratia sp. biofilm the former was biomineralized to produce a supported nanocrystalline Pd(0) catalyst, and this biofilm-Pd heterogeneous catalyst was then used to reduce C...
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PMID: 20506297
PDF is available here.
Abstract
We have used iTRAQ proteomics to identify and quantify alterations in global protein expression of T. forsythia during growth in a biofilm. This is the first proteomic study concentrating on biofilm growth in this key periodontal pathogen, and this study has identified several changes in protein exp...
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PMID: 20806225
PDF is available here.