Abstract
Changes in primary metabolism of lettuce, Lactuca sativa L. (cv. Cobham Green), induced by compatible interaction with the biotrophic oomycete pathogen Bremia lactucae Regel (race BL 16), under two intensities of illumination in the presence and absence of exogenous cytokinins were studied by chloro...
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PMID: 20471849
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
|
PMID: 20601497
PDF is available here.
Abstract
We adopted an Agrobacterium tumefaciens-mediated transient protein expression system in tobacco (Nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. Herein, we demonstrate that RPP1 associates with its cognate effector ARABIDOPSIS THALIANA RECOG...
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PMID: 20601497
PDF is available here.
Abstract
We investigated the basis for resistance to the downy mildew agent Hyaloperonospora arabidopsidis ex parasitica (Hpa) in a global sample of A. thaliana. We implemented a combined genome-wide mapping of resistance using populations of recombinant inbred lines and a collection of wild A. thaliana acce...
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PMID: 20479233
PDF is available here.
Abstract
We describe for the first time the molecular mechanism of resistance to a carboxylic acid amide (CAA) fungicide. We identified a family of four cellulose synthase (CesA) genes containing conserved domains that are found in all processive glycosyltransferases. Phylogenetic analysis revealed their clo...
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PMID: 20226261
PDF is available here.
Abstract
Four isolates of Peronophythora litchii with resistance to carboxylic acid amide (CAA) fungicides were selected on fungicide-amended agar. These isolates had various levels of resistance, as evidenced by their resistance factor (RF), which is the 50% effective concentration (EC(50)) value of a parti...
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PMID: 20373974
PDF is available here.
Abstract
Methyl salicylate (MeSA), which is synthesized in plants from salicylic acid (SA) by methyltransferases, has roles in defense against microbial and insect pests. Most of the MeSA that accumulates after pathogen attack is synthesized by benzoic acid/SA carboxyl methyltransferase 1 (AtBSMT1). To inves...
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PMID: 19958141
PDF is available here.
Abstract
We have assessed the implication of the transcriptional regulator OCP3 in SA- and JA-dependent induced defenses. Through a series of double mutant analyses, we conclude that SA-dependent defense signaling does not require OCP3. However, we found that ocp3 plants are impaired in a Pseudomonas fluores...
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PMID: 20836879
PDF is available here.
Abstract
Various fungal pathogens are used in Arabidopsis pathogen studies, including Fusarium oxysporum, Alternaria brassicicola, Botrytis cinerea, and others. The oomycete pathogen Peronospora parasitica has been used by several groups and is described in this protocol. Working with Peronospora is complica...
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PMID: 20147042
PDF is available here.
Abstract
A new maleimide-bearing compound, farinomalein (1), was isolated from the entomopathogenic fungus Paecilomyces farinosus HF599. The structure was determined on the basis of spectroscopic analyses and chemical conversion. Compound 1 showed potent activity (5 mug/disk) against the plant pathogenic Phy...
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PMID: 19670877
PDF is available here.
Abstract
Dual epidemics are defined as epidemics developing on two or several plant organs in the course of a cropping season. Agricultural pathosystems where such epidemics develop are often very important, because the harvestable part is one of the organs affected. These epidemics also are often difficult...
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PMID: 19594312
PDF is available here.
Abstract
We showed that, as for ABA, the elicitor-induced stomatal closure is mediated by reactive oxygen species (ROS) production in guard cells. In plants, we observed that the protection against downy mildew induced by some elicitors is probably not due only to effects on stomatal movements or to a guard-...
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PMID: 19589073
PDF is available here.
Abstract
Solvent extracts of 17 different basidiomycetes were tested for their ability to inhibit Sclerospora graminicola. Among those tested, only three basidiomycete crude extracts exhibited significant inhibition on pathogen sporulation, zoospore release and zoospore motility. In vitro, the chloroform ext...
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PMID: 19418357
PDF is available here.
Abstract
We summarize current knowledge about the population genetics of fungal and oomycete effector genes, focusing on recent studies that have used both spatial and temporal collections to assess the diversity and distribution of alleles and to monitor changes in allele frequencies over time. These studie...
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PMID: 19271952
PDF is available here.
Abstract
We documented colonization of eggs of three amphibian species, the wood frog (Rana sylvatica), the green frog (Rana clamitans), and the spotted salamander (Ambystoma maculatum), by water molds in the field and examined the interactive effects of road deicing salt and water molds, two known sources o...
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PMID: 18976747
PDF is available here.
Abstract
We studied if physical and chemical characteristics of the water from mosquito breeding sites affect pathogenicity, and the infectivity of zoospores of L. chapmanii. Water samples were taken from pools filled by rains, urban ditches with domestic waste water, pools filled by overflow from RÃo de...
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PMID: 19637714
PDF is available here.
Abstract
Manipulation of programmed cell death (PCD) is central to many host microbe interactions. Both plant and animal cells use PCD as a powerful weapon against biotrophic pathogens, including viruses, which draw their nutrition from living tissue. Thus, diverse biotrophic pathogens have evolved many mech...
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PMID: 19278553
PDF is available here.
BMC Microbiology (9 Suppl 1)suppl 1 2009
Abstract
We identify common features of the pathogenic processes of fungi and oomycetes and create a pathogenesis model using 256 newly developed and 38 extant GO terms, with an emphasis on the appressorium and signal transduction. This set of standardized GO terms provides a solid base to further compare an...
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PMID: 19278555
PDF is available here.
BMC Microbiology (9 Suppl 1)suppl 1 2009
Abstract
A wide diversity of plant-associated symbionts, including microbes, produce proteins that can enter host cells, or are injected into host cells in order to modify the physiology of the host to promote colonization. These molecules, termed effectors, commonly target the host defense signaling pathway...
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PMID: 19278551
PDF is available here.
Abstract
Two cDNAs named VvNPR1.1 and VvNPR1.2 were isolated from Vitis vinifera cv chardonnay, encoding proteins showing 55% and 40% identity to Arabidopsis NPR1 respectively. Constitutive expression of VvNPR1.1 and VvNPR1.2 monitored in leaves of V. vinifera cv chardonnay was found to be enhanced by treatm...
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PMID: 19432948
PDF is available here.
BMC Microbiology (9 Suppl 1)suppl 1 2009
Abstract
We review common themes among the nutrient acquisition strategies of a range of plant symbiotic microorganisms, including mutualistic symbionts, biotrophic pathogens that feed from living tissue, necrotrophic pathogens that kill host tissue, and hemibiotrophic pathogens that switch from biotrophy to...
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PMID: 19278554
PDF is available here.
Abstract
We describe the infectivity, virulence, cultivating conditions, and phylogenetic positions of naturally occurring oomycete parasites of Daphnia, invertebrates which play a major role in aquatic food webs. Daphnia pulex individuals were found dead and covered by oomycete mycelia when exposed to pond...
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PMID: 18992181
PDF is available here.
Abstract
The present study investigated the effect of chitosan seed priming on the induction of disease resistance in pearl millet against downy mildew disease caused by Sclerospora graminicola (Sacc.) Schroet.
Pearl millet seeds were primed with chitosan at different concentr...
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PMID: 18663698
PDF is available here.
Abstract
A population of Arabis alpina (Brassicaceae) growing in Saudi Arabia was observed to be infected for the first time by the Oomycete, Albugo candida. Both conventional chemical fixation and high pressure freezing followed by freeze substitution (HPF/FS) were used to prepare zoosporangia, intercellula...
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PMID: 18640046
PDF is available here.
Abstract
Eight pathotypes of Plasmopara halstedii were screened to investigate the occurrence of virions and the potential viral influence on the pathogenicity of the sunflower downy mildew pathogen. In 23 of 26 P. halstedii isolates derived from eight countries in Europe, North America and South America, vi...
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PMID: 19019006
PDF is available here.
Abstract
During the past two years the integrity of Albugo candida as the only species of Albugo parasitic to Brassicaceae has been challenged. The existence of two distinct species parasitic to Brassicaceae has been confirmed, to which a third species was added. For the purpose of further exploring the dive...
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PMID: 18951775
PDF is available here.
Abstract
We describe recent progress in characterizing RXLR-EER effectors and discuss why so many of these rapidly evolving proteins are encoded by the genomes of plant pathogenic oomycetes....
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PMID: 18819803
PDF is available here.
Abstract
We used these markers to study the genetic structure and the evolution of sunflower downy mildew races. Bayesian assignment analysis identified three genetically differentiated groups of isolates organized around the first three races described in France. Strong genetic substructuring according to g...
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PMID: 18450523
PDF is available here.
Abstract
We propose that CaPIMP1 plays distinct roles in both bacterial disease resistance and oomycete disease susceptibility....
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PMID: 18506481
PDF is available here.
Abstract
To manipulate host defences, plant pathogenic oomycetes secrete and translocate RXLR effectors into plant cells. Recent reports have indicated that RXLR effectors are translocated from the extrahaustorial matrix during the biotrophic phase of infection and that they are able to suppress PAMP-trigger...
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PMID: 18511334
PDF is available here.
Abstract
Following inoculation of host and nonhost plants with Plasmopara viticola, the grapevine downy mildew, a histological survey was undertaken to identify the stage where its development is contained in nonhosts and in resistant host plants. Three herbaceous nonhost species, Beta vulgaris, Lactuca sati...
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PMID: 18943253
PDF is available here.