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Edeine (0):
Recent article
Edeine
Abstract
A peptide antibiotic, edeine B(1), exerts a lethal action in Bacillus subtilis causing filamentous morphology. This antibiotic assumes to inhibit cell division by interacting with FtsZ and inhibiting FtsZ polymerization. The temperature-sensitive mutant ftsZ ts1 was shown to be hypersensitive to the...
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PMID: 20410587
PDF is available here.
PDF is available here.
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Abstract
I explain again why it is not valid to invoke a special mechanism of initiation based on the finding that edeine (at very low concentrations) does not inhibit the translation of a putative IRES from cricket paralysis virus. I explain why new assays, devised to rule out splicing in tests with dicistr...
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PMID: 17888589
PDF is available here.
PDF is available here.
Abstract
We have re-examined the wheat germ extract experiments that led to the Trojan horse model, incorporating a variety of controls. We report that (1) translation in vitro from the polyprotein AUG of TYMV RNA is unchanged after removal of the 3' TLS but is stimulated by the presence of a 5'-cap; (2) the...
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PMID: 17095542
PDF is available here.
PDF is available here.
Abstract
The edeines analogs were tested in several in vitro and in vivo assays using the mouse model, with edeine B (peptide W1) and cyclosporine A as reference compounds. The peptides displayed moderate, stimulatory effects on concanavalin A-induced (ConA-induced) splenocyte proliferation, whereas their ef...
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PMID: 17149559
PDF is available here.
PDF is available here.
Journal of Peptide Science (12)10 2006
Abstract
We describe the synthesis of four novel edeine A and D analogues in which the above-mentioned acid residue was replaced with the (3R, 4S)- or (3S, 4S)-4,5-diamino-3-hydroxypentanoic acid moiety. In one compound we also introduced into molecule the 3-N,N-dimethyl derivative of (S)-2,3-diaminopropanoi...
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PMID: 16847816
PDF is available here.
PDF is available here.
Abstract
We show that in the presence of 5 mM MgCl2, the HCV IRES can initiate translation by an alternative mechanism that does not require known initiation factors. Specifically, the HCV IRES was shown to initiate translation in a reconstituted system consisting only of purified 40S and 60S subunits, elong...
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PMID: 16556939
PDF is available here.
PDF is available here.
Nucleic Acids Research (33)5 2005
Abstract
We reassessed the mechanism accounting for the synthesis of the F protein by measuring the expression in cultured cells of a luciferase reporter gene with an insertion encompassing the IRES plus the beginning of the HCV-coding region preceding the luciferase-coding sequence. The insertion was such t...
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PMID: 15755749
PDF is available here.
PDF is available here.
Journal of Virology (79)2 2005
Abstract
We show here that the TSV IRES does not use this or any other AUG codon to initiate translation. Like the IRES in cricket paralysis virus (CrPV), the TSV IRES can assemble 80S ribosomes in the absence of initiation factors and can direct protein synthesis in a reconstituted system that contains only...
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PMID: 15613295
PDF is available here.
PDF is available here.
Journal of General Virology (85)Pt 5 2004
Abstract
Ongoing transcription in vitro of Tomato spotted wilt virus (TSWV) has previously been demonstrated to require the presence of reticulocyte lysate. This dependence was further investigated by testing the occurrence of transcription in the presence of two translation inhibitors: edeine, an inhibitor...
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PMID: 15105551
PDF is available here.
PDF is available here.
Molecular Cell (13)1 2004
Abstract
We show that base pair formation by addition of edeine inhibits tRNA binding to the P site by preventing codon-anticodon interaction and that addition of pactamycin, which rebreaks the base pair, can relieve this inhibition. In addition, edeine induces translational misreading in the A site, at leve...
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PMID: 14731399
PDF is available here.
PDF is available here.
Abstract
Analysis of the high resolution structure of the small subunit from Thermus thermophilus shed light on its inherent conformational variability and indicated an interconnected network of features allowing concerted movements during translocation. It also showed that conformational rearrangements may...
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PMID: 12370011
PDF is available here.
PDF is available here.
Abstract
We report here that a recently discovered IRES located in the genome of cricket paralysis virus can direct the efficient translation of a second URA3 cistron in dicistronic mRNAs in S. cerevisiae, thereby conferring uracil-independent growth. Curiously, the IRES functions poorly in wild-type yeast b...
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PMID: 11687653
PDF is available here.
PDF is available here.
EMBO Journal (20)8 2001
Abstract
We analyzed by X-ray crystallography the structures of three different complexes of the small ribosomal subunit of Thermus thermophilus with the A-site inhibitor tetracycline, the universal initiation inhibitor edeine and the C-terminal domain of the translation initiation factor IF3. The crystal st...
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PMID: 11296217
PDF is available here.
PDF is available here.
Journal of Antibiotics (52)4 1999
Abstract
Journal of Virology (71)4 1997
Abstract
An RNA-dependent RNA polymerase (RdRp) activity associated with the ribonucleoproteins of rice hoja blanca tenuivirus (RHBV) was detected and analyzed. Conditions for in vitro RNA synthesis and for coupled RNA synthesis-translation of RHBV were established. In both cases, synthesis of the viral and...
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PMID: 9060614
PDF is available here.
PDF is available here.
Nucleic Acids Research (25)2 1997
Abstract
Eukaryotic cellular mRNAs contain a cap at their 5'-ends, but some viral and cellular mRNAs bypass the cap-dependent mechanism of translation initiation in favor of internal entry of ribosomes at specific RNA sequences. Cap-dependent initiation requires intact initiation factor eIF4G (formerly eIF-4...
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PMID: 9016570
PDF is available here.
PDF is available here.
Abstract
A convenient and reliable multisample assay for the screening of inhibitors of the growth factor signalling enzyme phosphatidylinositol specific phospholipase C (PtdInsPLC) has been developed. Three naturally occurring peptide inhibitors of PtdInsPLC have been identified, myroridin K, streptothricin...
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PMID: 7916901
PDF is available here.
PDF is available here.
Abstract
We have produced radiolabeled eIF-4 gamma by in vitro transcription and translation. Despite the fact that eIF-4 gamma is predicted from the cDNA sequence to be 154 kDa, the major synthetic product migrated on SDS-polyacrylamide gel electrophoresis at 205 kDa. Although this is similar to the migrati...
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PMID: 8294456
PDF is available here.
PDF is available here.
Journal of Virology (66)2 1992
Abstract
We found that edeine, another inhibitor of protein synthesis, stimulated not only synthesis of S mRNAs but also that of the full-length S cRNAs. Taking into account the mode of action of this antibiotic, we discuss the results, which emphasize the crucial role of active ribosomes during bunyavirus t...
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PMID: 1731108
PDF is available here.
PDF is available here.
American Journal of Physiology -- Legacy Content (256)1 Pt 1 1989
Abstract
A cell-free system, which maintained a linear rate of protein synthesis for up to 20 min of incubation, was prepared from isolated rat hepatocytes. The rate of protein synthesis in the cell-free system was approximately 20% of the rate obtained in isolated hepatocytes or perfused liver. More than 70...
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PMID: 2463762
PDF is available here.
PDF is available here.
Abstract
Chemical footprinting shows that several classes of antibiotics (streptomycin, tetracycline, spectinomycin, edeine, hygromycin and the neomycins) protect concise sets of highly conserved nucleotides in 16S ribosomal RNA when bound to ribosomes. These findings have strong implications for the mechani...
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PMID: 2953976
PDF is available here.
PDF is available here.
Abstract
The r-proteins of an edeine-resistant mutant of Saccharomyces cerevisiae were compared to those of the wild-type strain by using two different two-dimensional electrophoretic techniques: (1) the Kaltschmidt-Wittmann method and, (2) the Kaltschmidt-Wittmann system, in the first dimension and the Na D...
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PMID: 3515127
PDF is available here.
PDF is available here.
Abstract
The effect of edeine on the translation of mRNA or poly(U)-directed polyphenylalanine synthesis has been studied in an edeine-resistant mutant of Saccharomyces cerevisiae under three different experimental conditions: in the whole lysate system, in a micrococcal-nuclease-treated lysate, and in a hig...
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PMID: 6389131
PDF is available here.
PDF is available here.
FEBS Letters (174)1 1984
Abstract
A cell-free protein synthesizing system from a mutant of Saccharomyces cerevisiae translated exogenous mRNA in the presence of 2 microM edeine, while a similar system from wild-type strain was completely inhibited by the drug. The mutant ribosomes showed an affinity for [125I]edeine comparable to th...
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PMID: 6381095
PDF is available here.
PDF is available here.
Abstract
Edeine inhibits poly(U)-dependent binding of tRNAPhe to the P and A sites simultaneously, both on 30S subunits and 70S ribosomes. Hence, edeine cannot be considered as antibiotic, "complementary" to tetracycline for selective adsorption of tRNA only to the P or to the A site. Further, edeine decreas...
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PMID: 6209550
PDF is available here.
PDF is available here.
Journal of Antibiotics (36)9 1983
Abstract
Journal of Antibiotics (36)8 1983
Abstract
Syntheses of the peptides with sequences postulated for active and inactive isomer of edeine D were carried out. The peptides obtained were identical with natural product in regard to chromatographic and electrophoretic properties. Biological data for synthesized compounds confirmed that in active a...
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PMID: 6415028
PDF is available here.
PDF is available here.
Journal of Antibiotics (36)7 1983
Abstract
The peptide antibiotic edeine F produced by Bacillus brevis Vm4, one of the components of edeine antibiotics complex, was isolated from a fermentation broth and was also obtained by amidination of edeine D. Edeine F is composed of amino acids: (S)-beta-phenyl-beta-alanine, (S)-isoserine, (S)-2,3-dia...
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PMID: 6615592
PDF is available here.
PDF is available here.
FEBS Letters (157)1 1983
Abstract
The 30 S subunit contains 2 sites for tRNA binding (Phe-tRNA, AcPhe-tRNA, tRNAPheOH) with the functional properties of D and A sites of the 70 S ribosome after attachment of 50 S subunit. The third (E) site specific for deacylated tRNA is introduced into 70 S ribosome by its 50 S subunit. The E-site...
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PMID: 6345196
PDF is available here.
PDF is available here.
FEBS Letters (153)2 1983
Abstract
Vaccinia cores inhibit translation in cell-free protein synthesis systems at two stages: initiation; and, as shown here, elongation. The former effect tends to obscure the latter. Elongation control could, however, be revealed as follows: when, in a reticulocyte of L-cell lysate, initiation was bloc...
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PMID: 6617871
PDF is available here.
PDF is available here.
Abstract
Acta Biochimica Polonica (29)3-4 1982
Abstract
Selectivity of amidination using ornithine as model amino acid was investigated in detail. The results obtained were taken advantage of for studying the reactions with other polyfunctional amino acids: beta-tyrosine, isoserine, 2,3-diaminopropionic acid, 2,6-diamino-7-hydroxyazelaic acid and with th...
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PMID: 7158170
PDF is available here.
PDF is available here.
Abstract
An N-terminal signal peptide has been identified on a precursor of the variant surface glycoprotein (VSG) of Trypanosoma brucei, synthesised both in vitro and in vivo. An ordered cyanogen bromide (CNBr) cleavage map was constructed for both mature VSG and for precursor VSG, and this allowed a size e...
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PMID: 6120455
PDF is available here.
PDF is available here.
Acta Virologica (25)3 1981
Abstract
The effects of edeine, hygromycin B and alpha-sarcin on the synthesis of virus-specific proteins and formation of infectious virions was studied in cells infected with fowl plague virus (FPV). The manifestation of the antiviral effect of edeine depended on the peculiarities of the FPV strains and th...
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PMID: 6115560
PDF is available here.
PDF is available here.
Journal of Antibiotics (34)1 1981
Abstract
Methods for the synthesis of esters and amides of edeine A were developed and a number of derivatives of this type was obtained and characterized. The antimicrobial activities of the derivatives are comparable to the activity of the native antibiotic and indicate that the presence of free carboxyl g...
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PMID: 7251506
PDF is available here.
PDF is available here.
Abstract
Abstract
Binding of eukaryotic initiation factor 4C (eIF-4C) to a 40-S initiation complex could be detected by analysis of assay mixtures for 40-S initiation complex formation on columns of Sepharose 6B and on sucrose gradients. On Sepharose 6B columns an almost stoichiometric binding of [14C]eIF-4C was obse...
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PMID: 7398638
PDF is available here.
PDF is available here.
Experimental Cell Research (124)2 1979
Abstract
Journal of Molecular Biology (134)3 1979
Abstract
Abstract
Three functionally related components that block peptide initiation have been identified in lysates of rabbit reticulocytes. The components function consecutively in a cascade type sequence of reactions to cause phosphorylation of eukaryotic peptide initiation factor 2 (eIF-2). The eIF-2 kinase acti...
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PMID: 223151
PDF is available here.
PDF is available here.
Abstract
The results of a series of experiments are interpreted to indicate that protein synthesis in reticulocyte lysates is not affected by the reticulocyte cAMP-dependent protein kinase. The catalytic subunit of this enzyme was isolated to apparent homogeneity. Also, the protein inhibitor of this protein...
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PMID: 218965
PDF is available here.
PDF is available here.
Journal of Virology (29)1 1979
Abstract
A number of translation inhibitors were tested for their effects on both control and encephalomyocarditis virus-infected mouse 3T6 cells. The virus-infected cells were specifically inhibited by gougerotin, edeine, and blasticidin S, whereas these drugs failed to penetrate into uninfected cells. Inhi...
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PMID: 219211
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (253)18 1978
Abstract
We suggest that in the absence of antibiotics, also, a 40 S ribosome might bind near the 5' terminus and then advance, stopping where it encounters the first AUG triplet. The effect of edeine might be to interfere with the AUG recognition process, thus allowing the 40 S ribosome to continue unhalted...
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PMID: 681367
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (253)18 1978
Abstract
Journal of Biological Chemistry (253)10 1978
Abstract
These results support the concept that Met-tRNAf binds to the native small ribosomal subunit before mRNA, and that processes involved in mRNA binding to the 43 SN preinitiation complex may be the rate-limiting step of 80 S initiation complex formation....
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PMID: 649579
PDF is available here.
PDF is available here.
Abstract
Three lines of evidence are presented indicating that GTP hydrolysis associated with eukaryotic peptide initiation occurs in the absence of 60 S subunits when methionyl-tRNAf is bound to 40 S ribosomal subunits. An enzyme fraction required for binding of methionyl-tRNAf to 40 S subunits and peptide...
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PMID: 246033
PDF is available here.
PDF is available here.
Bacteriological reviews (41)2 1977
Abstract
Biochemistry (46) 1977
Abstract
Abstract
We have examined the basis for the lag period preceding shut-off. The initiation factor that binds Met-tRNAf, previously shown to be rate-limiting in inhibited, heme-deficient lysates, is found to be used stoichiometrically in the presence of excess inhibitor. We suggest that a principal effect of t...
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PMID: 1068462
PDF is available here.
PDF is available here.
Abstract
1. Pyrocatechol violet inhibits the initiation of protein synthesis in reticulocyte lysates. It prevents the formation of 80-S initiation complexes by inhibiting the binding of mRNA to 40-S initiation complexes. 2. Pyrocatechol violet inhibits the hydrolysis of methionyl-tRNAmetf by reticulocyte lys...
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PMID: 938675
PDF is available here.
PDF is available here.
Abstract
Biochemistry (Washington) (15)10 1976
Abstract
The addition of cycloheximide to a thermosensitive conditional yeast mutant (ts-187) before and after transfer to the nonpermissive temperature (36 degrees C) for initiation of protein synthesis produces the uncoupling of the RNA and protein synthetic machineries. Since the drug can...
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PMID: 776214
PDF is available here.
PDF is available here.
Abstract
Abstract
We developed the translational system in order to look for the optimal ion-conditions of a DNA-dependent protein-synthesizing system. We found out that at the optimal MgCl2-concentration (6 mM) protein synthesis was strongly inhibited by Mangan ions which are required for transcription in yeast. If...
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PMID: 134559
PDF is available here.
PDF is available here.
Abstract
Abstract
1. Exogenous edeine inhibits the synthesis of DNA and protein, but not that of RNA, in extracts of edeine-producing Bacillus brevis Vm 4 cells. This is analogous to the effect of edeine on extracts obtained from edeine-sensitive cells. 2. Producer cells, in contrast to sensitive ones, are not permea...
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PMID: 1244852
PDF is available here.
PDF is available here.
Naturwissenschaften (62)9 1975
Abstract
Abstract
1. Cell-free systems prepared from Krebs II ascites cells incubated at 45 degrees C have a much lower endogenous activity than those from cells incubated at 37 degrees C. The endogenous activity is mainly due to completion of polypeptide chains initiated in the intact cell. The low activity of the 4...
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PMID: 1148247
PDF is available here.
PDF is available here.
Abstract
Edeine-synthesizing polyenzymes, associated with a complex of sytoplasmic membrane and DNA, were obtained from gently lysed cells of Bacillus brevis Vm4. The polyenzymes-membrane-DNA complex, isolated from dells intensively synthesizing edeines (18--20 h culture) contained edeine B. Edeine B was fou...
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PMID: 1148278
PDF is available here.
PDF is available here.