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Fibrinopeptide B (0):
Recent article
Fibrinopeptide B
Abstract
We suggest that both markedly slow polymerization and decreased plasmin resistance played causative roles in the hemorrhagic diathesis associated with this dysfibrinogen....
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PMID: 20019599
PDF is available here.
PDF is available here.
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Biochimica et Biophysica Acta (1784)9 2008
Abstract
We wanted to investigate the catalytic potential of MASP1 towards two major proteins involved in the clotting process, fibrinogen and factor XIII, and compare the activity directly with that of thrombin. We found that rMASP1 and thrombin cleave factor XIII A-chain and the fibrinogen beta-chain at id...
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PMID: 18456010
PDF is available here.
PDF is available here.
Abstract
Sodium ascorbate and histidine were employed to protect fibrinogen against modifications followed by a gamma-irradiation process that could potentially inactivate the blood-borne viruses in plasma-derived products. Fibrinogen was irradiated (50 kGy total dose, on dry ice) using a 60Co source. Sample...
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PMID: 17890955
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)31 2007
Abstract
We studied the interactions of the recombinant alphaC region (Aalpha221-610 fragment) and its subfragments, alphaC-connector (Aalpha221-391) and alphaC-domain (Aalpha392-610), between each other and with the recombinant (Bbeta1-66)2 and (beta15-66)2 fragments and NDSK corresponding to the fibrin(oge...
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PMID: 17630702
PDF is available here.
PDF is available here.
Abstract
An automated off-line liquid chromatography-matrix-assisted laser desorption ionization (LC-MALDI) interface capable of coupling both capillary and microbore LC separations with MALDI mass spectrometry (MS) and tandem mass spectrometry (MS/MS) has been developed. The interface is a combination of tw...
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PMID: 17605467
PDF is available here.
PDF is available here.
Haematologica (92)2 2007
Abstract
An asymptomatic, 29-year-old woman was referred to our hospital before surgery because in the basic study of hemostasis she showed a prolonged thrombin time (TT) and a normal reptilase time (RT). She had not received any anticoagulants so, to account for these abnormal results the presence of an inh...
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PMID: 17405747
PDF is available here.
PDF is available here.
Abstract
The relative abundances of fragment ions in electron capture dissociation (ECD) are often greatly affected by the secondary and tertiary structures of the precursor ion, and have been used to derive the gas-phase conformations of the protein ions. In this study, it is found that resonance ejection o...
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PMID: 16904337
PDF is available here.
PDF is available here.
Abstract
We implement the method using a quadrupole time-of-flight tandem mass spectrometer. The instrument is operated in TOF-MS mode that allows the ions from source region broadband-passing the first mass analyzer to enter the collision cell. Cone voltage and collision energy are investigated to optimize...
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PMID: 16970313
PDF is available here.
PDF is available here.
Abstract
Quantification of fibrinopeptides release is widely used to investigate fibrinogen activation, and standard chromatographic or capillary electrophoretic procedures are readily available. However, in the analyses of fibrinopeptide mixtures derived from the action of thrombin on human fibrinogen, a fe...
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PMID: 16953271
PDF is available here.
PDF is available here.
Biochemistry (Washington) (45)11 2006
Abstract
We reveal new details of the structure and present the results of molecular modeling of the fpA- and fpB-containing portions of the Aalpha and Bbeta chains, not identified in the complex, in both fibrinogen and protofibrils. The analysis of the results reveals that in fibrinogen the fpA-containing p...
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PMID: 16533041
PDF is available here.
PDF is available here.
Abstract
Electron capture dissociation was implemented in a digital ion trap without using any magnetic field to focus the electrons. Since rectangular waveforms are employed in the DIT for both trapping and dipole excitation, electrons can be injected into the trap when the electric field is constant. Follo...
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PMID: 16536438
PDF is available here.
PDF is available here.
Electrophoresis (26)24 2005
Abstract
We also demonstrated the use of this multiplex system in a 2-D LC configuration to increase sample loading and throughput for the analysis of biomarker samples of higher complexity. Variations in peptide abundance down to two-fold change were identified across salt fractions for spiked tryptic diges...
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PMID: 16358250
PDF is available here.
PDF is available here.
Electrophoresis (26)3 2005
Abstract
We describe a method for the simultaneous determination of the five fibrinopeptide forms derived from the thrombin-promoted activation of human fibrinogen by capillary zone electrophoresis (CZE). The fibrinopeptide mixture was first desalted by a solid-phase extraction (SPE) step. The analysis was p...
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PMID: 15690433
PDF is available here.
PDF is available here.
Biophysical Chemistry (112)2-3 2004
Abstract
The mechanisms involved in fibrin gel formation are reviewed. Furthermore, a new concept of the role of fibrinopeptide release in this process is presented.
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PMID: 15572242
PDF is available here.
PDF is available here.
Nippon rinsho. Japanese journal of clinical medicine (62 Suppl 12) 2004
Abstract
Abstract
We generated variant fibrinogens substituting alanine for gamma D316, gamma D318, gamma D320, and gamma K321. We measured thrombin-catalyzed polymerization and found normal polymerization with gamma K321A, no polymerization with gamma D316A, and, as reported by Lounes et al. in 2002, impaired polyme...
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PMID: 15311153
PDF is available here.
PDF is available here.
Abstract
We tested the hypothesis that venous thrombi significantly increase their thrombotic activity once they embolize into the high-flow circulation of the pulmonary arteries. Thrombotic activity was measured using an immunoassay that measures both fibrinopeptide B (FPB) as well as its most abundant meta...
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PMID: 15044200
PDF is available here.
PDF is available here.
Abstract
We synthesized 3 variant fibrinogens: gammaAsn308 changed to lysine (gammaN308K), isoleucine (gammaN308I), and alanine (gammaN308A). We measured thrombin-catalyzed polymerization by turbidity, fibrinopeptide release by high-performance liquid chromatography, and factor XIIIa-catalyzed cross-linking...
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PMID: 14764520
PDF is available here.
PDF is available here.
Abstract
We synthesized three fibrinogen variants, BbetaE397A, BbetaD398A, and BbetaD432A, with substitutions at positions identified in crystallographic studies as critical for binding the "B" peptide, Gly-His-Arg-Pro-amide (GHRPam), to the "b" polymerization site. We examined thrombin- and batroxobin-catal...
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PMID: 14992584
PDF is available here.
PDF is available here.
Abstract
We engineered variant fibrinogen gammaE132A in which calcium binding to the beta2-site was disrupted by replacing glutamic acid at gamma132 with alanine. We compared polymerization of gammaE132A to normal fibrinogen as a function of calcium concentration. Polymerization of gammaE132A at concentratio...
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PMID: 14992585
PDF is available here.
PDF is available here.
Biochemistry (Washington) (42)42 2003
Abstract
We found beta approximately 1, with corresponding normalized rate constants (K(a)) of 3.8, 4.2, 2.7, and 1.9 x 10(-5) [(NIHu/L)s](-1). Surprisingly, in TBC2.5 we found beta = 0.69, with an "average" K(a) of 3.5 x 10(-5) [(NIHu/L)s](-1). This effect disappeared [beta = 0.97, K(a) = 2.7 x 10(-5) [(NIH...
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PMID: 14567695
PDF is available here.
PDF is available here.
Abstract
We have developed a design that offers significant improvements in terms of ease of fabrication, durability, and maintenance of the integrity of the CE-separated analyte zones. Capillaries with different inside and outside diameters were evaluated to optimize the performance of the CE-MS system, res...
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PMID: 14588041
PDF is available here.
PDF is available here.
Abstract
Our objective was to investigate whether gammaA/gamma' differs in fibrin formation and structure from the more common variant gammaA/gammaA. Both variants were separated and purified by anion-exchange chromatography. Fibrin formation and clot structure of the variants and unfractionated fibrinogen w...
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PMID: 12663453
PDF is available here.
PDF is available here.
Abstract
Polydimethylcyclosiloxanes, an almost ubiquitous air contaminant, can interfere with nanoelectrospray analysis. The sensitivity of nanoelectrospray to these volatile air contaminants was demonstrated in this study. The intensity of the interfering ion signals caused by these compounds can be decreas...
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PMID: 12794873
PDF is available here.
PDF is available here.
Thrombosis Research (110)2-3 2003
Abstract
Urine FPBtot levels may help detect patients with PE and DVT....
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PMID: 12893031
PDF is available here.
PDF is available here.
Abstract
A novel, rugged capillary electrophoresis-electrospray ionization (CE-ESI) interface where the separation column, an electrical porous junction, and the spray tip are integrated on a single piece of a fused-silica capillary is described. ESI is accomplished by applying an electrical potential throug...
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PMID: 12705593
PDF is available here.
PDF is available here.
Life Sciences (71)10 2002
Abstract
We examined the mechanisms of the migration of vascular smooth muscle cells (SMCs) into fibrin gels, using an in vitro assay system. Cultured SMCs from bovine fetal aortic media migrated into fibrin gels prepared with thrombin, which cleaves both fibrinopeptides A and B from fibrinogen, without othe...
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PMID: 12095535
PDF is available here.
PDF is available here.
Biochemistry (Washington) (41)16 2002
Abstract
(1) a site in the gamma-module other than the C-terminus is critical for platelet aggregation and (2) "B-b" interactions have a role in protofibril formation....
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PMID: 11955079
PDF is available here.
PDF is available here.
Abstract
I shows a different fibrinopeptide release pattern and a mild factor V deficiency....
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PMID: 11914657
PDF is available here.
PDF is available here.
Abstract
We detected a peptide having a behavioral activity on mouse forced swimming from sera of healthy volunteers without affective and psychotic diseases. The amino acid sequence was GVNDNEEGF, which was found in the sequence of human beta-fibrinogen. The synthesized peptide also showed the behavioral ac...
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PMID: 11835988
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (276)30 2001
Abstract
We have characterized the ligand binding activity of SdrG, a fibrinogen-binding microbial surface component recognizing adhesive matrix molecules from S. epidermidis. Western ligand blot analysis showed that a recombinant form of the N-terminal A region of SdrG bound to the native Bbeta chain of fib...
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PMID: 11371571
PDF is available here.
PDF is available here.
Abstract
Fibrinogen Milano XII was detected in an asymptomatic Italian woman, whose routine coagulation test results revealed a prolonged thrombin time. Fibrinogen levels in functional assays were considerably lower than levels in immunologic assays. Polymerization of purified fibrinogen was strongly impaire...
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PMID: 11435303
PDF is available here.
PDF is available here.
Abstract
Fibrin monomer (0.003-0.1 micromol/L) stimulated a concentration-dependent increase in DNA synthesis of up to 10-fold, which could be inhibited by the peptide Bbeta15-42. The stimulation of DNA synthesis was highest for cells cultured on plastic and lowest for cells cultured on type I collagen polym...
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PMID: 11296341
PDF is available here.
PDF is available here.
Abstract
These results suggest that negative mode CID of multiply deprotonated ions provides useful structural information and can be worthwhile for highly acidic peptides that do not form positive ions in abundance....
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PMID: 11142354
PDF is available here.
PDF is available here.
Haemostasis (31)1 2001
Abstract
An abnormal fibrinogen was identified in a man with suspicious prolonged prothrombin time and a mild bleeding tendency. Coagulation studies showed marked prolonged thrombin and reptilase clotting times and a discrepancy between functional fibrinogen test and fibrinogen antigen. The rate of fibrinope...
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PMID: 11408744
PDF is available here.
PDF is available here.
Abstract
These results indicate 'B' and/or 'b' site of M-V fibrinogen play a more important role in thrombin-catalyzed fibrin polymerization than that of normal control fibrinogen....
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PMID: 11204560
PDF is available here.
PDF is available here.
Abstract
Fibrinopeptide A and B releasing enzyme, flavoviridiobin, was isolated from the venom of Trimeresurus flavoviridis using Q-Sepharose, CM-Cellulose, and Sephadex G-75 column chromatographies. Homogeneity was established by the formation of a single band in polyacrylamide gel electrophoresis, isoelect...
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PMID: 11126511
PDF is available here.
PDF is available here.
Biochemistry (Washington) (39)33 2000
Abstract
A novel thrombin-like enzyme (named contortrixobin) has been purified to homogeneity from the venom of Agkistrodon contortrix contortrix by affinity chromatography on arginine-Sepharose, anionic exchange chromatography, and HPLC. The complete amino acid sequence has been determined by Edman degradat...
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PMID: 10956019
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (275)33 2000
Abstract
We synthesized A'beta fibrinogen, replacing FpB with a fibrinopeptide A-like peptide, FpA' (G14V). Analyses of fibrinopeptide release from A'beta fibrinogen showed that FpA release and FpA' release were similar; the release of either peptide followed simple first-order kinetics. Specificity constant...
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PMID: 10837485
PDF is available here.
PDF is available here.
Thrombosis Research (99)3 2000
Abstract
Abstract
We investigated the molecular basis of hypofibrinogenemia in a man with a normal thrombin clotting time. Protein analysis indicated equal plasma expression of 2 different Bbeta alleles, and DNA sequencing confirmed heterozygosity for a new Bbeta235 P-->L mutation. Protein analysis also revealed a no...
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PMID: 10688828
PDF is available here.
PDF is available here.
Abstract
The myeloperoxidase-H2O2-chloride system (MPOS) is exploited by white blood cells to generate reactive oxygen species in many processes involved in the pathogenesis of inflammation and atherothrombosis. This, study investigated the biochemical and functional effects of alpha-thrombin oxidation by MP...
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PMID: 10739383
PDF is available here.
PDF is available here.
Abstract
We isolated the transglutaminase-modified, mostly monomeric form (92-96%) of fibrinogen with a N(epsilon)(gamma-glutamyl)lysine content of approximately 1.6 moles/mole of fibrinogen. The preparation was fully clottable by thrombin, but the rates of release of fibrinopeptides and clotting times were...
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PMID: 10618368
PDF is available here.
PDF is available here.
Surgery Today (30)9 2000
Abstract
We conducted a clinical study on patients with and without coagula and an experimental study on fibrinolytic activity in a rat model. The results of the clinical study showed that when coagula existed in the body cavity, the blood levels of the fibrin degradation products D-dimer and fibrinopeptide...
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PMID: 11039704
PDF is available here.
PDF is available here.
Thrombosis Research (96)5 1999
Abstract
Abstract
Journal of Biological Chemistry (274)32 1999
Abstract
We have developed methods to quantitate fibrinopeptides (FPs) and soluble and insoluble Fg/Fn products during the tissue factor induced clotting of whole blood. Significant FPA generation (>50%) occurs prior to visible clotting (4 +/- 0.2 min) coincident with factor XIII activation. At this time Fg...
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PMID: 10428872
PDF is available here.
PDF is available here.
Abstract
From the snake venom of Agkistrodon acutus, two proteases, acuthrombin-A and acuthrombin-C, were isolated and purified to homogeneity. They can cleave the human fibrinogen to release the fibrinopeptide A and fibrinopeptide B with specific activity of 120 and 370 NIH units/mg, respectively; the fibri...
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PMID: 10484747
PDF is available here.
PDF is available here.
Abstract
I (1-14), and epidermal mitosis inhibiting pentapeptide yielded much larger deprotonated ion signals, [M - H]-, than protonated ions, [M + H]+. This may be due to their absence of basic residues, coupled with their arrays of acidic residues. The PSD fragmentation of the peptide negative ions showed...
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PMID: 9868910
PDF is available here.
PDF is available here.
Biochemistry (Washington) (37)44 1998
Abstract
We synthesized a recombinant fibrinogen, Aalpha251 fibrinogen, that lacks the alphaC domain. We examined thrombin-catalyzed fibrinopeptide release and found that the rate of FpB release from Aalpha251 fibrinogen was 2.5-fold slower than FpB release from normal fibrinogen, while the rate of FpA relea...
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PMID: 9799505
PDF is available here.
PDF is available here.
Biochemistry (Washington) (37)39 1998
Abstract
We have examined the requirement for Phe at this position by constructing a variant recombinant fibrinogen with a conservative substitution of tyrosine for phenylalanine, Aalpha F8Y fibrinogen. We found that the variant fibrinopeptide A (F8Y 1-16) was cleaved by thrombin, in contrast to the lack of...
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PMID: 9753458
PDF is available here.
PDF is available here.
Abstract
Fibrinopeptide B (M(r) 1552.58) was employed as a calibration compound for matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) post-source decay (PSD) fragment ion analysis in the negative mode. Experiments were performed by using both continuous and delayed extraction, with the m...
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PMID: 9879369
PDF is available here.
PDF is available here.
Abstract
We report the first direct detection of a fibrinogen mutation by electrospray ionisation mass spectrometry. The propositus, from a family with a history of thrombosis, came to attention after a pulmonary embolism subsequent to a spontaneous abortion. Prolonged thrombin (41 s) and reptilase times (26...
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PMID: 9423799
PDF is available here.
PDF is available here.
Abstract
We have examined the conversion of fibrinogen to fibrin, comparing the recombinant with the plasma protein. We examined the kinetics of (1) thrombin-catalyzed fibrinopeptide release, (2) thrombin-catalyzed polymerization of fibrinogen, (3) the polymerization of fibrin monomers, and (4) FXIIIa-cataly...
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PMID: 9192765
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (271)20 1996
Abstract
The structure of a fibrin gel depends on the nature of the fibrinogen activation products produced by thrombin and the physical condition under which assembly occurs. Two different structures of the intermediate fibrin protofibril have been proposed, the production of which requires different extent...
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PMID: 8662661
PDF is available here.
PDF is available here.
Biochemistry (Washington) (35)14 1996
Abstract
I to the fast form of thrombin in the transition state is in the second-order rate constant for association, k(l). In the case of fibrinogen, the values of k(l) for interaction with the fast and slow forms at 25 degrees C are 19 +/- 4 and 2.5 +/- 0.3 microM(-1) s(-1), with an activation energy of ab...
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PMID: 8605191
PDF is available here.
PDF is available here.
Abstract
We believe that plasma FPA and desarginine FPB levels should not be used as specific markers of thrombin activity during the course of thrombolytic therapy and suppression of elevated FPA values by heparin should not be accepted as evidence that heparin is effective in this clinical setting....
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PMID: 8743172
PDF is available here.
PDF is available here.
Abstract
Immunohistochemistry was applied to AMeX-fixed sections of twelve cases of gastric carcinoma obtained at surgical resection to explore the occurrence and distribution of fibrin deposits in situ. Fibrinogen was distributed in abundance throughout perivascular zones and in the connective tissue of the...
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PMID: 9112657
PDF is available here.
PDF is available here.