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Phosphopeptides (0):
Recent article
Phosphopeptides
Abstract
Major difficulties in phosphoprotein analysis relate to the presence of a huge number of nonphosphorylated proteins and to the wide concentration dynamic range among them. In order to overcome the analysis complexity, specific clean-up and highly efficient enrichment procedures are mandatory prior t...
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PMID: 20978974
PDF is available here.
PDF is available here.
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Abstract
We utilized three different types of TiO(2) nanoparticles (NPs) namely TiO(2)-dopamine, TiO(2)-CdS and bare TiO(2) NPs as multifunctional nanoprobes for the rapid enrichment of phosphopeptides from tryptic digests of α- and β-casein, milk and egg white using a simplified procedure in MALDI-TOF-MS....
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PMID: 20928925
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
We validated the MS method by determining the IC(50) value of imatinib, an Abl inhibitor for clinical treatment of chronic myelogenous leukaemia (CML). The obtained IC(50) value (234 nM) is consistent with that determined by ELISA (291 nM). Then, six analogues of imatinib synthesized in our laborato...
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PMID: 20835477
PDF is available here.
PDF is available here.
Abstract
We report the crystal structure at 2.4 Å resolution of the amino-terminal domain (residues 30-340) of human symplekin in a ternary complex with the Pol II carboxy-terminal domain (CTD) Ser 5 phosphatase Ssu72 (refs 7, 10-17) and a CTD Ser 5 phosphopeptide. The N-terminal domain of symplekin...
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PMID: 20861839
PDF is available here.
PDF is available here.
Proteomics (10)19 2010
Abstract
LC combined with MS/MS analysis of complex mixtures of protein digests is a reliable and sensitive method for characterization of protein phosphorylation. Peptide retention times (RTs) measured during an LC-MS/MS run depend on both the peptide sequence and the location of modified am...
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PMID: 20815086
PDF is available here.
PDF is available here.
Abstract
We demostrate that the phosphopeptide enrichment method based on an online continuous pH gradient in a strong anion exchange column (SAX method) is highly complementary to the method based on titanium dioxide (TiO2) affinity enrichment. Moreover, we found that the flow-through fraction of either SAX...
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PMID: 20681634
PDF is available here.
PDF is available here.
Abstract
Prostate tissue microarray analysis indicates that Ack1 Tyr284 phosphorylation correlates positively with disease progression and negatively with the survival of prostate cancer patients. Interestingly, neither pTyr267-AR expression nor its transcriptional activation was affected by anti-androgens i...
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PMID: 20623637
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Abstract
A label free fluorescence method for rapid detection of the presence of phosphorylated peptides/proteins in sample solutions using functional magnetic nanoparticles (MNPs) as the detection probes is demonstrated.
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PMID: 20574590
PDF is available here.
PDF is available here.
Plant Physiology (153)3 2010
Abstract
We show that in Arabidopsis, the A-domain is not simply degraded but that it accumulates as a soluble, phosphorylated protein separated from Toc159. However, the physiological relevance of this process is unclear. The data show that the A-domain of Toc159 as well as those of its homologs Toc132 and...
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PMID: 20457805
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
|
PMID: 20374526
PDF is available here.
PDF is available here.
Journal of Molecular Biology (399)4 2010
Abstract
We determined the crystal structure of a phosphopeptide derived from melanoma antigen recognized by T cells-1 (pMART-1), selectively expressed by human melanomas, in complex with HLA-DR1. The structure revealed that the phosphate moiety attached to the serine residue at position P5 of pMART-1 is ava...
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PMID: 20417641
PDF is available here.
PDF is available here.
Cancer Research (70)12 2010
Abstract
We use biochemical and cell-based transcriptional assays to assess the structural and functional defects associated with a large set of 117 distinct BRCA1 missense variants within the essential BRCT domain of the BRCA1 protein that have been documented in individuals with a family history of breast...
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PMID: 20516115
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular me...
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PMID: 20223255
PDF is available here.
PDF is available here.
British Journal of Nutrition (103)12 2010
Abstract
Suboptimal vitamin D status among the South Asian UK population is widely reported; however, its impact on bone health is unclear. The aim of the present study was to conduct a comparative investigation of vitamin D status in postmenopausal South Asian (SA) and Caucasian (C) women and its relationsh...
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PMID: 20102676
PDF is available here.
PDF is available here.
Abstract
We developed a novel sequential phosphopeptide enrichment method, using magnetic iron oxide (Fe(3)O(4)) and titanium dioxide (TiO(2)) particles, to detect mono- and multi-phosphorylated peptides. In the first step, phosphopeptides were captured on Fe(3)O(4) particles. In a subsequent step, any resid...
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PMID: 20411586
PDF is available here.
PDF is available here.
Abstract
A novel algorithm, PILOT_PTM, has been developed for the untargeted identification of post-translational modifications (PTMs) on a template sequence. The algorithm consists of an analysis of an MS/MS spectrum via an integer linear optimization model to output a rank-ordered list of PTMs that best ma...
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PMID: 20103568
PDF is available here.
PDF is available here.
Journal of Proteomics (73)6 2010
Abstract
Interferon regulatory factor 3 (IRF-3) is a critical transcription factor that regulates innate immune responses against viral infection. Upon infection, IRF-3 is activated through phosphorylation of Ser/Thr residues in its C-terminal domain by the kinases, IKK-i and/or TBK-1. This p...
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PMID: 20170763
PDF is available here.
PDF is available here.
Chemical Communications (46)13 2010
Abstract
Ti-hexagonal mesoporous silica (Ti-HMS) with high titanium content has been synthesized. The Ti-HMS has been applied as a potential adsorbent for the selective capture of phosphopeptides, due to the strong affinity interaction between the incorporated titanium in the framework and the phosphoryl gro...
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PMID: 20234929
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We performed a global quantitative analysis of the Arabidopsis phosphoproteome in response to a time course of treatments with various plant hormones using phosphopeptide enrichment and subsequent mass accuracy precursor alignment (MAPA). The use of three time points, 1, 3 and 6 h, in combination wi...
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PMID: 20374526
PDF is available here.
PDF is available here.
Abstract
We examined the regulatory interactions of a serine/threonine phosphatase (BA-Stp1), serine/threonine kinase (BA-Stk1) pair in Bacillus anthracis. B. anthracis STPK101, a null mutant lacking BA-Stp1 and BA-Stk1, was impaired in its ability to survive within macrophages, and this correlated with an o...
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PMID: 19915022
PDF is available here.
PDF is available here.
Voprosy Virusologii (55)3 2010
Abstract
Five phosphodipeptides were synthesized; two of them (H-Lys-Ala(P) and H-Pro-Ala(P) had interferon-induced activity. These dipeptides at millimolar concentrations (10(-4)) and 10(-5) M) induced the synthesis of late (40-hour) interferon in human peripheral blood lymphocytes. The dipeptides H-Lys-Ala...
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PMID: 20608081
PDF is available here.
PDF is available here.
Abstract
We systematically assessed matrix combinations of 2, 6-dihydroxyacetophenone (DHAP) and diammonium hydrogen citrate (DAHC), and demonstrated that the low ratio DHAP/DAHC matrix was more effective in enhancing the ionization of phosphopeptides. Low femtomole level of phosphopeptides from the tryptic...
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PMID: 20339515
PDF is available here.
PDF is available here.
Abstract
We report an initial attempt to characterize the phosphoprotein content of serum. To accomplish this, we developed a method in which phosphopeptides are enriched from digested serum proteins and analyzed by LC-MS/MS using LTQ-Orbitrap (CID) and LTQ-ETD mass spectrometers. With this approach, we iden...
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PMID: 19824718
PDF is available here.
PDF is available here.
Proteomics (9)24 2009
Abstract
We have developed a simple and efficient method for phosphopeptides enrichment, which employs a biphasic phosphate-binding tag (Phos-tag)/C18 tip consisting of overlaid Phos-tag on the C18 resin in a pipet tip. The improvement in selectivity for phosphopeptides was achieved by using a 40% ACN soluti...
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PMID: 19834909
PDF is available here.
PDF is available here.
Abstract
We are targeting its SH2 domain to prevent docking to cytokine and growth factor receptors and subsequent signaling. One of the important elements of the recognition sequence, pTyr-Xxx-Xxx-Gln, is glutamine. We incorporated novel Gln mimics into a lead peptide, pCinn-Leu-Pro-Gln-NHBn, and found that...
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PMID: 19728728
PDF is available here.
PDF is available here.
Abstract
We analyzed the position and conservation of large numbers of phosphorylation sites for the cyclin-dependent kinase Cdk1 in the budding yeast Saccharomyces cerevisiae. We combined specific chemical inhibition of Cdk1 with quantitative mass spectrometry to identify the positions of 547 phosphorylatio...
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PMID: 19779198
PDF is available here.
PDF is available here.
Molecular Biotechnology (43)1 2009
Abstract
We demonstrate that IMAC beads possess a strong adsorption of nucleic acids, especially single-stranded or single-stranded-region-containing nucleic acids, leading to approximately 50% loss of phosphopeptides during the process of IMAC enrichment. Therefore, nucleic acids must be removed from protei...
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PMID: 19412670
PDF is available here.
PDF is available here.
Abstract
We report the identification of minimal phosphopeptides that specifically interact with the PBD of human PLK1, but not those of the closely related PLK2 and PLK3. Comparative binding studies and analyses of crystal structures of the PLK1 PBD in complex with the minimal phosphopeptides revealed that...
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PMID: 19597481
PDF is available here.
PDF is available here.
Bioorganic Chemistry (37)4 2009
Abstract
Phosphopeptide pTyr-Glu-Glu-Ile (pYEEI) has been introduced as an optimal Src SH2 domain ligand. Peptides, Ac-K(IDA)pYEEIEK(IDA) (1), Ac-KpYEEIEK (2), Ac-K(IDA)pYEEIEK (3), and Ac-KpYEEIEK(IDA) (4), containing 0-2 iminodiacetate (IDA) groups at the N- and C-terminal lysine residues were synthesized...
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PMID: 19539345
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (284)30 2009
Abstract
We show that increased IP(3) receptor (IP(3)R) sensitivity is initiated at the germinal vesicle breakdown stage of maturation, which correlates with maturation promoting factor (MPF) activation. Extensive phosphopeptide mapping of the IP(3)R resulted in approximately 70% coverage and identified thre...
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PMID: 19473987
PDF is available here.
PDF is available here.
Abstract
We identified the first phosphorylated MHC class I ligands from tumor tissue (renal cell carcinoma) and, by comparison to healthy renal tissue, found one Brf1-derived ligand as potentially tumor-associated. We further discovered the first natural phosphorylated MHC class II ligands. They revealed se...
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PMID: 19415920
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the method is particularly useful for the characterization of large phosphopeptides, including those with multiple phosphorylation sites, as extensive series of c' and z fragment-ions were observed. Finally, we have applied a directed tandem mass spectrometric workflow using incl...
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PMID: 19435317
PDF is available here.
PDF is available here.
Proteomics (9)10 2009
Abstract
We present phosphoproteomes of two ecologically diverse non-enteric Gram-negative bacteria captured by a nanoLC-MS-based approach combined with a novel phosphoenrichment method. While the phosphoproteome data from the two species are not very similar, the results reflect high similarity to the previ...
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PMID: 19405024
PDF is available here.
PDF is available here.
Abstract
We performed enzyme purification to obtain a novel L-amino acid ligase from Bacillus subtilis NBRC3134, a microorganism producing peptide-antibiotic rhizocticin. Rhizocticins are dipeptide or tripeptide antibiotics and commonly possess L-arginyl-L-2-amino-5-phosphono-3-cis-pentenoic acid. The purifi...
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PMID: 19352016
PDF is available here.
PDF is available here.