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Polylysine (0):
Recent article
Polylysine
Abstract
We investigated the delivery of vascular endothelial growth factor (VEGF) and the effect on in vitro cultured human umbilical vein endothelial cells (HUVECs), in an attempt to provide experimental basis for promoting the vascularization of tissue-engineered bone grafts. A mechanical stimulator was d...
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PMID: 21341993
PDF is available here.
PDF is available here.
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Abstract
Implanted polymeric materials, such as medical devices, provoke the body to initiate an inflammatory reaction, known as the foreign body response (FBR), which causes several complications. In this study, polyurethane (Tecoflex®, PU) surface modified with the nonionic surfactant Tween80® (PU/T80) a...
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PMID: 20648535
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)11 2010
Abstract
We evaluate the adhesive behaviour of skeletal muscle cells (C2C12 myoblasts) during the initial steps of spreading on layer-by-layer films of controlled stiffness made of poly(L-lysine) and hyaluronan as model biomaterial surfaces for muscle tissue engineering. We show that integrin clustering, int...
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PMID: 20601233
PDF is available here.
PDF is available here.
Abstract
We show that the Saccharomyces cerevisiae Ltn1 RING-domain-type E3 ubiquitin ligase acts in the quality control of non-stop proteins, in a process that is mechanistically distinct but conceptually analogous to that performed by ssrA: Ltn1 is predominantly associated with ribosomes, and it marks nasc...
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PMID: 20835226
PDF is available here.
PDF is available here.
Abstract
We have studied the effects of successive hydration on the (15)N labeled side chain amino groups of solid poly-L-lysine (PLL) in the presence of acids. Generally, hydration leads to the formation of local "ionic fluid" phases composed by flexible side chain ammonium groups, acid anions and small amo...
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PMID: 20648266
PDF is available here.
PDF is available here.
Optics Express (18)18 2010
Abstract
We present a "holographic module", a simple optical path that can be combined with commercial scanheads for simultaneous imaging and uncaging with structured two-photon light. The present microscope is coupled to two independently tunable lasers and has two principal configurations: holographic imag...
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PMID: 20940765
PDF is available here.
PDF is available here.
Abstract
We developed polylysine based DNA nanoparticles (DNA NPs) that contain disulfide linkage in the carrier and demonstrated that this reducible DNA NP enhances in vitro gene transfer via an extracellular mechanism. Polylysine was conjugated through an N-terminal cysteine to a polyethylene glycol chain...
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PMID: 20438780
PDF is available here.
PDF is available here.
Abstract
Well-defined AB2 Y-shaped miktoarm star copolymers of PNIPAM-b-(PZLL)2 and PNIPAM-b-(PLL)2 were synthesized through the combination of atom transfer radical polymerization (ATRP), ring-opening polymerization (ROP), and click chemistry, where PNIPAM, PZLL, and PLL are poly(N-isopropylacrylamide), pol...
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PMID: 20536122
PDF is available here.
PDF is available here.
Biomaterials (31)19 2010
Abstract
The blood-brain barrier is the major obstacle that prevents diagnostic and therapeutic drugs being delivered to the central nervous systems in order to exert their effects. Specific ligand-receptor binding mediated endocytosis is one of the possible strategies to cross this barrier. A 30-amino-acid...
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PMID: 20382424
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
We propose pulsed-gating as a facile method to extend the operation window of LGFETs to |VG| > 1 V. A comparison between simulation and experimental results reveals that at voltages in excess of 1 V, the LGFET sensing mechanism has a contribution from two factors: electrostatic gating as well as cap...
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PMID: 20464024
PDF is available here.
PDF is available here.
Abstract
Most commonly used Drosophila cell lines adhere only loosely to the substrate. For some purposes (e.g., time-lapse photography and cytological examination of living cells), it is desirable to fix the growing cells more firmly to the substrate. As described in this protocol, this can be accomplished...
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PMID: 20516171
PDF is available here.
PDF is available here.
Abstract
PEGylated poly-L-lysine DNA nanoparticles are composed of plasmid DNA compacted with poly-L-lysine conjugated with polyethylene glycol (PEG). They are soluble and stable in saline and tissue fluids, transfect nondividing cells, display minimal toxicity, and are effective in vivo and in humans. Moreo...
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PMID: 20439407
PDF is available here.
PDF is available here.
Abstract
To improve the stability of a head-tail type polycation (PAMAM dendron-PLL), which is composed of a polyamidoamine (PAMAM) dendron head and poly(l-lysine) (PLL) tail blocks, as a gene vector against serum proteins, the PEGylation of the PAMAM dendron head was examined using physicochemical and trans...
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PMID: 20201491
PDF is available here.
PDF is available here.
Abstract
epsilon-Poly-L-lysine (epsilon-PL), a natural biomacromolecule having a broad spectrum of antibacterial activity, was grafted on the wool fiber via the acyl transfer reaction catalyzed by microbial transglutaminase (mTGase) to develop a new strategy for antibacterial functionalization of proteinous...
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PMID: 19649747
PDF is available here.
PDF is available here.
Abstract
We analyzed the effect of poly ICLC on pulmonary responses to influenza and respiratory syncytial virus (RSV) infections in the cotton rat (Sigmodon hispidus) model. Viral replication, pulmonary inflammation, and expression of IFN, TLR, and chemokines were monitored and compared. Antiviral effect of...
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PMID: 20038196
PDF is available here.
PDF is available here.
Abstract
A poly-L-lysine (PLL)/bacteriorhodopsin-embedded purple membrane (bR-PM) multilayer film has been successfully constructed by a layer-by-layer (LbL) assembly process to enhance the photoelectric response of bR. The assembly conditions were investigated and optimized. The PLL/bR-PM adsorption process...
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PMID: 20056227
PDF is available here.
PDF is available here.
Abstract
We report on the characterization of two members of soybean LEA IV proteins, basic GmPM1 and acidic GmPM28, by circular dichroism and Fourier transform infrared spectroscopy. The spectra of both proteins revealed limited defined secondary structures in the fully hydrated state. Thus, the soybean LEA...
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PMID: 20071374
PDF is available here.
PDF is available here.
Abstract
A new epsilon-PL-producing strain TUST-2 was isolated from the soil collected from Hainan province, China. Chemotaxonomic and morphological characteristics of the isolate were typical of strain of the genus Streptomyces. The strain TUST-2 was found to belong to Streptomyces diastatochromogenes by co...
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PMID: 20387461
PDF is available here.
PDF is available here.
Abstract
We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathoge...
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PMID: 19948852
PDF is available here.
PDF is available here.
Abstract
SPL7013 Gel applied vaginally once daily for 7 days at concentrations of 0.5% to 3% was safe and well tolerated in healthy, sexually abstinent women, with no evidence of systemic toxicity or absorption....
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PMID: 19823111
PDF is available here.
PDF is available here.
Abstract
We describe the steps required during the in situ fractionation of adherent and non-adherent mammalian cells on microscope coverslips. Protein visualization can be achieved using specific antibodies or fluorescent fusion proteins and fluorescence microscopy. Antibodies and/or fluorescent dyes that a...
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PMID: 20689508
PDF is available here.
PDF is available here.
Abstract
We investigated the interactions of poly-l-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in...
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PMID: 20463944
PDF is available here.
PDF is available here.
Abstract
Our data might provide an important insight into the mechanism of pathogenesis of SARS-CoV and these properties might be therapeutically advantageous....
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PMID: 20231782
PDF is available here.
PDF is available here.
Abstract
Magnetic resonance (MR) is one of the most widely used imaging modalities in contemporary medicine to obtain images of pathological areas. Still, there is a big effort to facilitate the accumulation of contrast in the required zone and further increase a local spatial concentration of a contrast age...
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PMID: 20072891
PDF is available here.
PDF is available here.
Abstract
I ribosome-inactivating proteins (RIPs), gene-encoding plasmids, adenovirus, oligonucleotides, and the chemotherapeutic bleomycin. PCI has also been shown to enhance the treatment effect of targeted therapeutic macromolecules. The present protocol describes PCI of an epidermal growth factor receptor...
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PMID: 20552345
PDF is available here.
PDF is available here.
Abstract
We developed a protocol to measure the bending rigidity of filamentous rod-shaped bacteria. Forces are applied with an optical trap, a microscopic three-dimensional spring made of light that is formed when a high-intensity laser beam is focused to a very small spot by a microscope's objective lens....
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PMID: 20421864
PDF is available here.
PDF is available here.
Abstract
Our results show a significant enantiospecific preference of d-GLU over l-GLU at PEMs containing PLL and of d-ASC over l-ASC at PEMs containing PEI-m. No such enantiospecific preference was found for nonchiral PEMs containing PEI. The enantiospecificity of PEMs of PLL/PSS toward l/d-GLU could be sig...
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PMID: 20356170
PDF is available here.
PDF is available here.
Abstract
We have developed nanoscaled 3D scaffolds consisting of growth factor-loaded heparin/poly(l-lysine) nanoparticles (NPs) attached to the surface of polymeric microspheres via polyionic complex methods. Growth factor-loaded NPs were simply produced as polyelectrolyte complexes with diameters of 100-20...
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PMID: 19996706
PDF is available here.
PDF is available here.
Abstract
Otolaryngologia Polska (63)7 2009
Abstract
The histological and macroscopic examinations indicated that both materials developed in this study have properties similar to cartilaginous tissue and seem to be good for her restoration. Although the quickest tissue regeneration was found after implantation of PLG-Hyal....
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PMID: 20564904
PDF is available here.
PDF is available here.
Abstract
We have recently developed and reported an amphiphilic dendritic poly(L-lysine)-b-poly(L-lactide)-b-dendritic poly(L-lysine) D(2)-PLLA-D(2) with two-generation PLL dendrons and a PLLA block. In this work, we continued to explore the roles of dendritic PLL generation in DNA binding and intracellular...
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PMID: 19586045
PDF is available here.
PDF is available here.
Abstract
EPL was assembled as functional dimers and capable of binding DNA plasmids in both an EPL protein and plasmid DNA concentration-dependent manner. EPL targeted plasmid DNA to the high-affinity receptor for IgE on cell surfaces and increased the model gene uptake/expression. The EPL-DNA complexes were...
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PMID: 19423156
PDF is available here.
PDF is available here.
Abstract
Extracellular stability of electrostatically formed siRNA polyplexes is a significant concern in the delivery process. To overcome the risk of polyplex dissociation in the extracellular environment, siRNA was covalently incorporated into a pH- and redox-responsive polymer conjugate....
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PMID: 19348503
PDF is available here.
PDF is available here.
Abstract
In the present work, a series of composite films were produced from chitosan/poly-L-lysine blend solutions. The surface topography, chemistry, and wettability of composite films were characterized by atomic force microscopy (AFM), attenuated total reflection Fourier transform infrared (ATR-FTIR) spe...
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PMID: 18431777
PDF is available here.
PDF is available here.
Abstract
We need to determine the fundamental material properties that influence NSC behavior. To investigate the role of material properties on NSCs, we synthesized a library of 52 hydrogels composed of poly(ethylene glycol) and poly(L-lysine) (PLL). This library of hydrogels allows independent variation of...
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PMID: 18435406
PDF is available here.
PDF is available here.
Abstract
It is feasible to treat experimental diabetes by transplantation of encapsulated Langerhans islets without immunosuppression....
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PMID: 19579813
PDF is available here.
PDF is available here.
Abstract
SPL7013 is the sodium salt of a sulfonated dendrimer that has potent antiviral properties. VivaGel, a topical gel containing 3% (wt/wt) SPL7013, is in development as a vaginal microbicide. BufferGel is a Carbopol-based acidic buffering gel that enhances the natural protective action of the vagina to...
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PMID: 19040181
PDF is available here.
PDF is available here.
Abstract
We hypothesized that poly(L-lysine) (PLL), an analog of the protein catalyst responsible for silica formation in primitive marine species, would remain functional on dentin. PLL was applied to dentin discs along with a pre-hydrolyzed silica precursor, tetramethyl orthosilicate (TMOS). Discs were ana...
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PMID: 19407161
PDF is available here.
PDF is available here.
Abstract
We therefore investigated the structure of H1/siRNA complexes. Five commonly used methods characterizing the resulting assemblies such as retardation gels, static and dynamic light scattering, reduction of ethidium bromide fluorescence, analytical ultracentrifugation, and electron microscopy were us...
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PMID: 18566913
PDF is available here.
PDF is available here.
Abstract
We find that the film thickness, after the deposition of a given number of layer pairs, reaches a minimum for x values lower than 0.1 (the position of this minimum could not be more precisely localized) and that the film thickness at intermediate values of x is the same as that of films built at the...
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PMID: 19708245
PDF is available here.
PDF is available here.
Abstract
In this study, thermoresponsive xyloglucan hydrogel scaffolds were investigated as candidates for neural tissue engineering of the spinal cord. The hydrogels were optimized to provide similar mechanical properties to that of native spinal cord, although also being functionalized through the immobili...
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PMID: 18404707
PDF is available here.
PDF is available here.
Abstract
We studied the possibility to simultaneously image contrast agents based on two different MRI contrast mechanisms: chemical exchange saturation transfer (CEST) and enhancement of T2 relaxation. Various amounts of superparamagnetic iron oxide (SPIO) nanoparticles were mixed with a fixed concentration...
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PMID: 19189296
PDF is available here.
PDF is available here.
Abstract
Through the use of superparamagnetic iron oxide nanoparticles (SPIONs), fast delivery of DNA into adherent and suspended cells could be achieved by the mediation of a strong impulsed magnetic field. Mammalian cells were well transfected with enhanced green fluorescent protein gene. T...
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PMID: 19438016
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (134)4 2009
Abstract
Oxygen is a major actor of many physiological, biological and industrial processes; as such, its monitoring is of paramount importance. The effects of protein biofouling on dissolved oxygen measurements are described. The consequences of protein adsorption on electrode kinetics and mass transport we...
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PMID: 19305931
PDF is available here.
PDF is available here.
Biomaterials (30)10 2009
Abstract
pH-responsive polymers have been synthesised by grafting l-valine (PV-75), l-leucine (PL-75) and l-phenylalanine (PP-75) onto the pendant carboxylic acid moieties of a pseudo-peptide, poly(l-lysine iso-phthalamide), at a stoichiometric degree of substitution of 75 mol%. The effect of such modificati...
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PMID: 19138797
PDF is available here.
PDF is available here.
Physical Review Letters (102)11 2009
Abstract
We numerically study the length dependence in the bending stiffness of alpha helices, coiled coils, and a linear chain model. As the length approaches what we named the critical buckling length lc, the chain appears to become increasingly more flexible. This is due to weak nonbonded attractions that...
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PMID: 19392240
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (89)11 2009
Abstract
OBJECTIVE: To evaluate the feasibility of the injectable thermosetting chitosan-glycerophosphate-fibroblast (C-GP-FB) hydrogel as an embolizing material of intracranial aneurysm. METHODS: 2% chitosan solution and 56% glycerophosphate solution were mixed in different volume ratio to detect the pH, co...
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PMID: 19595098
PDF is available here.
PDF is available here.
Biophysical Journal (96)6 2009
Abstract
We used a hydrodynamic membrane tube extrusion technique to characterize the mechanical interaction between the plasma membrane and the underlying cortical cytoskeleton. Cells were anchored on 4.5 microm beads coated with polylysine (PL) to obtain nonspecific cell adhesion or with an antibody agains...
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PMID: 19289070
PDF is available here.
PDF is available here.
Abstract
We analyzed the efficiency of cationic lipid-mediated plasmid DNA transfection using adherent and acutely dissociated neuronal suspensions derived from embryonic mouse cortical tissue. Compared to transfections using adherent cultures, the in-tube procedure enhanced the delivery of a GFP reporter pl...
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PMID: 19014969
PDF is available here.
PDF is available here.
Abstract
We hypothesized that developing zebrafish could be used to identify and distinguish these different types of toxicity. Embryos were exposed to aqueous suspensions of CdSe(core)/ZnS(shell) QDs functionalized with either poly-L-lysine or poly(ethylene glycol) terminated with methoxy, carboxylate, or a...
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PMID: 19350942
PDF is available here.
PDF is available here.
Molecular and Cellular Biochemistry (323)1-2 2009
Abstract
These results suggest that FN negatively modulates eNOS in an alpha(5)beta(1) integrin-p38 MAPK-dependent pathway....
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PMID: 19052844
PDF is available here.
PDF is available here.
Optics Express (17)5 2009
Abstract
We demonstrate a sensitive DNA biosensor based on a long period grating (LPG) formed by a photolithograph process on the surface of a side-polished fiber. The biomolecules of the biosensor were immobilized on the silica surface between LPG patterns. The resonance wavelength was red-shifted after the...
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PMID: 19259227
PDF is available here.
PDF is available here.
Abstract
We report that the transfection ability of these vectors in their own right is relatively low, because the low-generation number limits the endosomal buffering capacity. Importantly, however, we demonstrate that when applied in combination with Lipofectamine 2000, a vector from the cationic lipid fa...
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PMID: 19194595
PDF is available here.
PDF is available here.
Abstract
We have shown that the capture and release of cefazolin was pH-induced and could be controlled, and the developed antibiotic-incorporated polypeptide multilayer nanocoatings could prevent Staphylococcus aureus colonization thus showing great potential for preventing implant-associated infection.
Cop...
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PMID: 18161817
PDF is available here.
PDF is available here.
Abstract
We also found that perturbation from spin coating significantly influences the silicification dynamics. The ability to catalyze nano- to micron-sized mineral with different morphologies using polypeptides could have numerous dental applications including, sealing of dentin tubules, in situ reinforce...
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PMID: 18161782
PDF is available here.
PDF is available here.
Abstract
We report the analogy between the self-assembly properties of amphiphilic phospholipids and the similar behavior observed for quantum dot (CdSe/CdS)-diblock copolypeptide hybrid systems, and the effect of the self-assembly on secondary structures of the polypeptides. At neutral pH, the diblock copol...
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PMID: 19072205
PDF is available here.
PDF is available here.
Abstract
Amphiphilic triblock copolymers of methoxy-poly(ethylene glycol)-poly(L-lactide)-poly(L-lysine) (MPEG-b-PLLA-b-PLL) (Mn=8540-22 240) were synthesized through the ring-opening polymerization of Nepsilon-(Z)-lysine-N-carboxyanhydrides (N(epsilon)-(Z)-Lys-NCA) using MPEG-b-PLLA-NH2 as a macroinitiator....
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PMID: 19063715
PDF is available here.
PDF is available here.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques (12)3 2009
Abstract
IR results show that the antisymmetric PO2- vibration of DNA (at 1224 cm-1) shifts toward lower frequencies in complexation with PEI or PLLys (these polymers are able to transfect DNA). By contrast, the antisymmetric PO2- vibration of DNA in presence of PDAMA or dextran (these polymers are poor in D...
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PMID: 20067709
PDF is available here.
PDF is available here.
Abstract
Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert...
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PMID: 19325638
PDF is available here.
PDF is available here.
Abstract
A general method for the preparation of biodegradable microcapsules capable of antigen inclusion is suggested. Multilayer microcapsules were obtained by the method of level-by-level sorption of various polyelectrolytes (alginate, poly-L-lysine, kappa-carrageenan, and chitosan and dextran derivatives...
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PMID: 19377529
PDF is available here.
PDF is available here.