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Antifreeze Proteins (4):
Recent article
Antifreeze Proteins
Abstract
We have studied the hyperactive insect AFP from Tenebrio molitor (TmAFP) with the (17)O magnetic relaxation dispersion (MRD) method, which selectively monitors the rotational motion and exchange kinetics of water molecules on picosecond-microsecond time scales. The global hydration behavior of TmAFP...
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PMID: 20668761
PDF is available here.
PDF is available here.
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Abstract
An insect antifreeze protein gene Mpafp149 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis dzungarica. Sequence analysis revealed that this gene encoding a protein of 120 amid acids and this protein showed 65-76% homology with other insect antifreeze proteins, the ded...
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PMID: 19562508
PDF is available here.
PDF is available here.
Abstract
We designed to introduce the Thp gene into the plants. The physiological and biochemical effect of high-lever expression of the modified Choristoneura fumiferana Thp (ThpI) in Arabidopsis thaliana plants was analyzed. Under low temperature stress, the ThpI transgenic plants exhibited stronger growth...
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PMID: 19728155
PDF is available here.
PDF is available here.
Abstract
We reasoned that species with low-temperature adaptations should also be present, although rare, in more temperate environments. In consequence, the challenge then becomes one of selecting for rare microbes present in a larger community. Following the introductory commentary, we demonstrate that bot...
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PMID: 20662383
PDF is available here.
PDF is available here.
FEBS Journal (277)2 2010
Abstract
We report a new fungal antifreeze protein from another psychrophile, Antarctomyces psychrotrophicus. We examined its biochemical properties and thermal hysteresis activity, and compared them with those of the T. ishikariensis antifreeze protein. The antifreeze protein from A. psychrotrophicus was pu...
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PMID: 20030710
PDF is available here.
PDF is available here.
Abstract
For a long time, the cryopreservation of gametes and embryos remained a major hurdle for the clinicians and scientists in terms of success. However, recent technical advancement in the field of cryobiology has opened up various options for freezing gametes and embryos at different developmental stag...
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PMID: 19573301
PDF is available here.
PDF is available here.
[The secondary structure of winter-wheat-bran antifreeze protein determined by FTIR and CD spectrum]
Abstract
I and III of the winter wheat bran antifreeze protein were fitted by the Gauss peak to show the characteristic peaks of the alpha-helix, beta-sheet, beta-turn and random coil. The CD spectrum was evaluated by the Chen-Yang principle and analyzed by the least squares method. Furthermore, the differen...
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PMID: 19798935
PDF is available here.
PDF is available here.
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences (367)1894 2009
Abstract
Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of prote...
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PMID: 19376768
PDF is available here.
PDF is available here.
Abstract
We have modified classical LSW theory, allowing for the derivation of inhibition rate constants. It was found that the investigated synAFGPmi inhibit ice recrystallization at concentrations down to approximately 3 microg mL(-1) or, equivalently, approximately 1 micromol L(-1) for the largest synAFGP...
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PMID: 19708116
PDF is available here.
PDF is available here.
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Biomacromolecules (10)2 2009
Abstract
A series of structurally diverse polymers, containing either peptide or vinyl-derived backbones, was tested for ice recrystallization inhibition activity, which is commonly associated with antifreeze (glyco)proteins. It was revealed that only polymers bearing hydroxyl groups in the s...
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PMID: 19072300
PDF is available here.
PDF is available here.
Abstract
Cryobiology (57)3 2008
Abstract
Five genes coding for ice-active proteins were identified from an expressed sequence tag database of Lolium perenne cDNA libraries. Each of the five genes were characterized by the presence of an N-terminal signal peptide, a region enriched in hydrophilic amino acids and a leucine-rich region in fou...
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PMID: 18835384
PDF is available here.
PDF is available here.
Abstract
We are still far from identifying the critical elements behind over-wintering success and how some species can regularly survive temperatures below -20 degrees C. Molecular biology is the most recent tool to be added to the insect physiologist's armoury. With the public availability of the genome se...
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PMID: 18584182
PDF is available here.
PDF is available here.
Abstract
The strategy for cold-hardiness and water balance features of two closely related families of Coleoptera, Cerambycidae and Chrysomelidae, were investigated. Cerambycids were freeze-avoiding with low supercooling points, whereas chrysomelids froze at high temperatures and were tolerant to freezing. H...
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PMID: 18563418
PDF is available here.
PDF is available here.
ChemPhysChem (9)11 2008
Abstract
The ability of antifreeze glycoproteins to inhibit clathrate-hydrate formation is studied using DFT. A 5(12) cavity, dodecahedral (H(2)O)(20), and the AATA peptide are used to model the inhibitor-clathrate interaction. The presence of AATA in the vicinity of the water cavities not only leads to the...
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PMID: 18618535
PDF is available here.
PDF is available here.
Abstract
These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein....
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PMID: 18598029
PDF is available here.
PDF is available here.
Abstract
We report the total chemical synthesis of the mirror image forms of sfAFP (i.e., L-sfAFP, the native protein, and D-sfAFP, the native protein's enantiomer). The predicted 81 amino acid residue polypeptide chain of sfAFP contains Cys residues at positions 1, 13, 28, and 43 and was prepared from four...
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PMID: 18598026
PDF is available here.
PDF is available here.
Plant Cell Reports (27)8 2008
Abstract
To elucidate the function of antifreeze protein from Microdera puntipennis dzhungarica for freezing stress tolerance in plant, the construct of MpAFP149 gene with the signal peptide sequence responsible for secreting the native MpAFP149 into the apoplast space under control of a cauliflower mosaic v...
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PMID: 18528694
PDF is available here.
PDF is available here.
Abstract
I AFP, TTTT, and the low molecular weight fraction glycoprotein, AFGP8, with dimyristoylphosphatidylcholine membranes above and below the gel-fluid phase transition temperature. In contrast to previous studies in fluid phase bilayers these experiments have provided direct information regarding both...
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PMID: 18449532
PDF is available here.
PDF is available here.
Biophysical Journal (95)1 2008
Abstract
We fluorescently tagged sbwAFP and visualized it on the surface of ice crystals using fluorescence microscopy. SbwAFP accumulated at the six prism plane corners and the two basal planes of hexagonal ice crystals. In contrast, fluorescently tagged fish type III AFP did not adhere to the basal planes...
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PMID: 18339740
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)23 2008
Abstract
We confirmed that the decrease in the growth velocity of ice was attributable to the melting point depression caused by the Gibbs-Thomson effect. Simulation results suggested that the growth of ice surrounding the AFP is needed to promote stable AFP binding to the interface and subsequent ice growth...
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PMID: 18476736
PDF is available here.
PDF is available here.
Biophysical Journal (94)11 2008
Abstract
Interfacial adsorption from two antifreeze proteins (AFP) from ocean pout (Macrozoarces americanus, type III AFP, AFP III, or maAFP) and spruce budworm (Choristoneura fumiferana, isoform 501, or cfAFP) were studied by neutron reflection. Hydrophilic silicon oxide was used as model su...
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PMID: 18234809
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)19 2008
Abstract
We show that the flexible F3C water model reproduces properties of water in the solid phase (ice I(h)), the supercooled liquid phase, and at the ice/liquid water interface. Additionally, the hydration of the type III AFP from ocean pout was studied as a function of temperature. Hydration waters on t...
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PMID: 18336017
PDF is available here.
PDF is available here.
Plant Physiology (147)1 2008
Abstract
A cold-responsive chitinase gene, BiCHT1, was isolated from bromegrass (Bromus inermis) 'Manchar' suspension cells. BiCHT1 messenger RNA was detected at low levels in nonstressed bromegrass cells, whereas its accumulation was induced by incubation at 10 degrees C and 4 degrees C as detected by north...
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PMID: 18359848
PDF is available here.
PDF is available here.
Biochemical Journal (411)1 2008
Abstract
We have cloned,expressed and characterized a 322-amino-acid region of the protein where the antifreeze activity is localized that shows similarity to the RTX (repeats-in-toxin) family of proteins. The recombinant protein requires Ca2+ for structure and activity, and it is capable of depressing the f...
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PMID: 18095937
PDF is available here.
PDF is available here.
Abstract
These results demonstrate that a normal reproductive lifespan can be restored by orthotopic transplantation of AFP transgenic-vitrified ovary....
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PMID: 17874447
PDF is available here.
PDF is available here.
Abstract
Abstract
We recently discovered a hyperactive AFP in the winter flounder and related species that is many-fold more active than other fish AFPs. Like the 3-4-kDa type I AFPs, it is alanine-rich and highly helical, but this 17-kDa protein is considerably larger and forms a dimer. We have sequenced the cDNA en...
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PMID: 18225917
PDF is available here.
PDF is available here.
Abstract
We compared the effectiveness of a dry collection cup (with an insecticide killing strip) to a wet collection cup (containing antifreeze) for use with Lindgren multiple-funnel traps in catching several common species of bark and wood-boring beetles, and their associates in southern pine forests. All...
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PMID: 18330123
PDF is available here.
PDF is available here.
B M B Reports (41)1 2008
Abstract
We examined its importance for the secondary KDN-9-P synthetic activity. The chimeric protein retained almost half of the ability of the human enzyme for KDN-9-P synthesis while the NeuNAc-9-P production was reduced to less than 10%. Data from the homology modeling and the effect of divalent ions an...
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PMID: 18304454
PDF is available here.
PDF is available here.
Cryo letters (29)6 2008
Abstract
We investigated insect species that are active during winter and we demonstrated the ability of the mecopteran Boreus hiemalis (Mecoptera, Boreidae), the wingless fly Chionea sp. (Diptera, Limoniidae) and cantharid larvae (Coleoptera, Cantharidae) to accumulate sugars in their haemolymph to survive...
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PMID: 19280052
PDF is available here.
PDF is available here.
Cryo letters (29)4 2008
Abstract
Larvae of Rhagium mordax empty their guts in preparation for the winter, which alone may enable the larvae to supercool down to -20 degree C or below. This should be sufficient for the larvae to over winter in Denmark if they can prevent inoculation. Antifreeze proteins (AFP) prevent inoculation in...
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PMID: 19137192
PDF is available here.
PDF is available here.
Abstract
Antifreeze proteins (AFPs) are a group of proteins that protect organisms from deep freezing temperatures and are expressed in vertebrates, invertebrates, plants, bacteria, and fungi. The nuclear magnetic resonance, x-ray structure, and many spectroscopic studies with AFPs have been instrumental in d...
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PMID: 18322856
PDF is available here.
PDF is available here.
Iubmb Life (60)1 2008
Abstract
In the Antarctic, fishes of dominant suborder Notothenioidei have evolved in a unique thermal scenario. Phylogenetically related taxa of the suborder live in a wide range of latitudes, in Antarctic, sub-Antarctic and temperate oceans. Consequently, they offer a remarkable opportunity to study the ph...
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PMID: 18379990
PDF is available here.
PDF is available here.
Abstract
We purified many kinds of antifreeze proteins with high activity from the leaves of Ammopiptanthus mongolicus by several biochemical techniques. The antifreeze activities of these AFPs were measured by both osmometry and differential scanning calorimetry, and the inhibition of growth of ice crystals...
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PMID: 18320468
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (111)51 2007
Abstract
We monitored the supercooled and frozen states of the film and measured the thickness of the quasi-liquid layer (QLL) at the ice/prism interfaces. The AFGP structure was determined for the liquid, supercooled, and frozen states of the solution film using the amide I band spectra. No noticeable diffe...
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PMID: 18047311
PDF is available here.
PDF is available here.
Abstract
Abstract
The effects of concentrated carrot protein (CCP) containing 15.4% (w/w) carrot (Daucus carota) antifreeze protein on texture properties of frozen dough and volatile compounds of crumb were studied. CCP supplementation lowered the freezable water content of the dough, resulting in some beneficial eff...
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PMID: 17935294
PDF is available here.
PDF is available here.
Abstract
Surface patterning of antifreeze glycoprotein fraction 8 (AFGP 8) via a solvent evaporation method is reported here. In this process, lines of AFGP 8 particles and gridlike patterns were formed as as result of the receding of the droplet contact line and the accumulation of the solute during evapora...
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PMID: 17927221
PDF is available here.
PDF is available here.
Abstract
Antifreeze proteins (AFP) and antifreeze glycoproteins (AFGP) lower the freezing point of marine fish plasma non-colligatively by specifically adsorbing to certain surfaces of ice crystals, modifying their structure and inhibiting further growth. While the freezing point is lowered, the melting poin...
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PMID: 17707669
PDF is available here.
PDF is available here.
Abstract
We used earlier. First, TaAFP was concentrated by a phosphate buffer, on the basis of its strong hydrophilicity that was validated by thermal gravimetric analyses and a surface hydrophobicity analysis. Second, TaAFP was trapped in ice crystals for its specific ice-binding capacity, which was proved...
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PMID: 17715897
PDF is available here.
PDF is available here.
Biophysical Journal (93)5 2007
Abstract
We calculate the free energy difference between the hydrophilic and hydrophobic faces of the protein interacting with ice. Furthermore, we identify three properties of Type I "antifreeze" proteins that discriminate among these two orientations of the protein at the ice/water interface. The three pro...
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PMID: 17526572
PDF is available here.
PDF is available here.
Insect Molecular Biology (16)4 2007
Abstract
Antifreeze proteins (AFPs) are encoded by approximately 17 genes in the spruce budworm, Choristoneura fumiferana. Northern analysis using 6 different cDNA probes showed isoform-specific patterns that varied during development. Transcripts for the majority of isoforms were most abundant in the second...
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PMID: 17651234
PDF is available here.
PDF is available here.
Abstract
The tissue-specific changes in protein synthesis were tracked in relation to the seasonal metabolic depression in cunner (Tautogolabrus adsperus). In vivo protein synthesis rate and total RNA content were determined in liver, white muscle, brain, heart, and gill during periods of normal activity bef...
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PMID: 17379844
PDF is available here.
PDF is available here.
Abstract
The antifreeze ability of glutathione was evaluated on the basis of its thermodynamic characteristics and protection of baker's yeast during cryopreservation at -30 degrees C. The thermodynamic characteristics and protection of baker's yeast of glutathione were similar to those of known antifreeze p...
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PMID: 17508758
PDF is available here.
PDF is available here.
Biophysical Journal (92)10 2007
Abstract
We observed the binding of the AFP to ice. This was accomplished by monitoring the presence of GFP-AFP on the surface of ice crystals several microns in diameter using fluorescence microscopy. The lack of recovery of fluorescence after photobleaching of the GFP component of the surface-bound GFP-AFP...
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PMID: 17325008
PDF is available here.
PDF is available here.
Abstract
Biomacromolecules (8)5 2007
Abstract
We describe the in vitro effects and interactions of C-linked AFGP analogue 1 and native AFGP 8. Our studies reveal that AFGP 8 is cytotoxic to human embryonic liver and human embryonic kidney cells at concentrations higher than 2 and 0.63 mg/mL, respectively, whereas lower concentrations are not to...
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PMID: 17411090
PDF is available here.
PDF is available here.
Abstract
We have obtained the cDNA and genomic sequences of additional T. molitor isoforms. They show variation in the number of repeats (from 6 to 10) which can largely be explained by recombination at various TCT motifs. In addition, phylogenetic comparison of the AFPs from the two beetles suggests that ge...
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PMID: 17443386
PDF is available here.
PDF is available here.
Abstract
Our analysis demonstrates an inverse correlation between AFGP 8 concentration and average crystal size with consistently small, but detectable differences in average crystal size at the edge and centre of the ice wafer. Sensitivity analysis using Monte Carlo re-sampling methods indicate that measuri...
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PMID: 17239343
PDF is available here.
PDF is available here.