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Azurin (0):
Recent article
Azurin
Abstract
A method for the electrical contacting of redox enzymes that obtain oxidizing or reducing equivalents from small electron-transfer proteins is demonstrated. The electrochemical contacting of redox enzymes through their immobilization onto electrode supports offers great potential for technological a...
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PMID: 20397667
PDF is available here.
PDF is available here.
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Biochemical Journal (426)3 2010
Abstract
We show by X-ray crystallography that in FU-h of KLH1 (keyhole-limpet-haemocyanin isoform 1) the structure of the tail domain is cupredoxin-like but contains no copper. The copper-free domain 3 in arthropod haemocyanin subunits has also recently been reinterpreted as being cupredoxin-like. We propos...
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PMID: 20025608
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)4 2010
Abstract
Analysis of the Rhodobacter sphaeroides 2.4.3 genome revealed four previously unidentified sequences similar to the binding site of the transcriptional regulator NnrR. Expression studies demonstrated that three of these sequences are within the promoters of genes, designated paz, norEF, and cdgA, in...
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PMID: 19966004
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
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PMID: 19701722
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
|
PMID: 19701722
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
|
PMID: 19701722
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
|
PMID: 19701722
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
|
PMID: 19701722
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
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PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2010
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Extremophiles (14)1 2009
Abstract
Phenotypic and genotypic analysis was carried out on four iron- and sulfur-oxidizing acidophilic bacteria (the "NO-37 group") isolated from different parts of the world. 16S rRNA phylogeny showed that they are highly related to each other, but are less related to the type strain of A...
|
PMID: 19787416
PDF is available here.
PDF is available here.
Abstract
Our work demonstrates that time-resolved IR spectroscopy and emission anisotropy of Re(I) carbonyl-diimine complexes are powerful probes of molecular dynamics at or around the surfaces of proteins and protein-protein interfacial regions....
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PMID: 19639996
PDF is available here.
PDF is available here.
Abstract
I is predicted to bind Cu(2+) using three conserved residues (C41 and 103, and E66) and M101. Of these, only one (C103) is conserved in classical cupredoxins where it also acts as a Cu ligand. Site II most probably binds Fe(3+) and consists of four well conserved surface Glu residues. Phylogenetic a...
|
PMID: 19701722
PDF is available here.
PDF is available here.
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory (113)30 2009
Abstract
In this study an attempt has been made to investigate the effect of metal ion substitution on the structure and spectra of azurin using two-layer ONIOM (our own N-layered integrated molecular orbital + molecular mechanics) approach. It is evident from the results that the overall structure of the pr...
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PMID: 19572691
PDF is available here.
PDF is available here.
Abstract
We have constructed a function-based model of the Fox1 protein which replicates hCp's six copper-site ligand arrays with an overall root mean square deviation of 1.4 A. Analysis of this model has led also to assignment of motifs in Fox1 that are unique to ferroxidases, the strongest evidence to date...
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PMID: 19023602
PDF is available here.
PDF is available here.
Biophysical Chemistry (139)1 2009
Abstract
The hybrid system obtained by conjugating the protein azurin, which is a very stable and well-described protein showing a unique interplay among its electron transfer and optical properties, with 20-nm sized gold nanoparticles has been investigated. Binding of azurin molecules to gold nanoparticle su...
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PMID: 18938024
PDF is available here.
PDF is available here.
Abstract
Searching for "intelligence": Azurin-PNIPAM conjugates were prepared by site-directed mutagenesis followed by protein reconstitution by using imidazole-conjugated poly(N-isopropylacrylamides). The polymer-bound imidazole acts as a ligand in the active site of the blue copper protein azurin. The bioc...
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PMID: 19199311
PDF is available here.
PDF is available here.
Abstract
The successful modeling of metalloproteins is an important step in understanding their structure and function. Toward this goal, models of the noncoupled copper centers found in the enzymes peptidyl alpha-hydroxylating monooxygenase (PHM), dopamine beta-monooxygenase (DBM), and nitrite reductase (Ni...
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PMID: 18830721
PDF is available here.
PDF is available here.
Abstract
We have investigated the hybrid system constituted by the redox copper protein Azurin integrated with the semiconductor indium tin oxide (ITO) coated on quartz substrate. The system appears to be a good candidate for bio-sensing and bio-optoelectronics applications, especially due to the coupling be...
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PMID: 18457941
PDF is available here.
PDF is available here.
Physical Review Letters (101)15 2008
Abstract
We show that beyond a transition distance (2-3 A in water and 6-7 A in proteins), fluctuation contributions to the mean-squared donor-to-acceptor tunneling matrix element are likely to dominate over the average matrix element. Even though fluctuations dominate the tunneling mechanism at larger dista...
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PMID: 18999647
PDF is available here.
PDF is available here.
Current Microbiology (57)4 2008
Abstract
We analyzed the expression, by real-time PCR, of the eight genes from the rus operon when A. ferrooxidans LR was grown in the presence of iron (control) and then kept in contact with chalcopyrite (CuFeS(2)) and covellite (CuS). A small decrease in rus operon gene expression was observed in the prese...
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PMID: 18665419
PDF is available here.
PDF is available here.
Abstract
We report a kinetic and thermodynamic study to characterize the process, taking into consideration that the unfolded state undergoes an irreversible reaction....
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PMID: 18515056
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)33 2008
Abstract
This investigation represents a first attempt to gain a quantitative estimate of the effects of the anions sulfate, citrate, acetate, chloride and thiocyanate on the thermodynamic stability (DeltaG degrees) of a model globular protein in ice at -15 degrees C. The method, based on guanidinium chlorid...
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PMID: 18665632
PDF is available here.
PDF is available here.
Biophysical Journal (95)2 2008
Abstract
Changes in flexibility and structural stability of Pseudomonas aeruginosa azurin in response to cavity-creating mutations were probed by the phosphorescence emission of Trp-48, which was deeply buried in the compact hydrophobic core of the macromolecule, and by measurements of guanidinum hydrochlori...
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PMID: 18424505
PDF is available here.
PDF is available here.
Abstract
We report transient optical and infrared spectroscopic experiments that quantify the extent to which an intervening tryptophan residue can facilitate electron transfer between distant metal redox centers in a mutant Pseudomonas aeruginosa azurin. Cu(I) oxidation by a photoexcited Re(I)-diimine at po...
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PMID: 18583608
PDF is available here.
PDF is available here.
Abstract
Abstract
We report herein the observation of pH-dependent transformation between blue T1 copper, red T2 copper, and the native purple Cu(A) centers of nitrous oxide reductase (N2OR) from Paracoccus denitrificans. The blue and red copper centers form initially before they are transformed into purple Cu(A) cen...
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PMID: 18535143
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)18 2008
Abstract
We have investigated the effect of a physiological electron donor to cytochrome cd(1), the copper protein pseudoazurin, on the mechanism of nitrite reduction by the enzyme. Our data clearly show that initially oxidized pseudoazurin causes rapid further turnover by the enzyme to give a final product...
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PMID: 18310770
PDF is available here.
PDF is available here.
Abstract
Our resonance Raman and MCD spectra demonstrate that the low pH and H120A mutant forms are essentially identical and are the perturbed forms of the completely delocalized high pH CuA site. However, in going from high pH to low pH, a seven-line hyperfine coupling pattern associated with complete delo...
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PMID: 18348522
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (112)14 2008
Abstract
This study represents the first attempt to gain a quantitative estimate of the protective influence of sugars (sucrose and trehalose) and polyols (sorbitol and glycerol) on the thermodynamic stability (DeltaG degrees ) of a protein in low-temperature part-frozen aqueous solutions. The method, based...
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PMID: 18341329
PDF is available here.
PDF is available here.
Abstract
The metabolism of Thiobacillus ferrooxidans involves electron transfer from the Fe+2 ions in the extracellular environment to the terminal oxygen in the bacterial cytoplasm through a series of periplasmic proteins like Rusticyanin (RCy), Cytochrome (Cyt c4), and Cytochrome oxidase (CcO). The energy...
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PMID: 18282009
PDF is available here.
PDF is available here.
Abstract
We present the results of the first quantum chemical investigations of 1H NMR hyperfine shifts in the blue copper proteins (BCPs): amicyanin, azurin, pseudoazurin, plastocyanin, stellacyanin, and rusticyanin. We find that very large structural models that incorporate extensive hydrogen bond networks...
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PMID: 18314973
PDF is available here.
PDF is available here.
Abstract
S K-edge X-ray absorption, UV-vis absorption, magnetic circular dichroism (MCD), and resonance Raman spectroscopies are used to investigate the electronic structure differences among WT, M121SeM, and C112SeC Pseudomonas aeruginosa (P.a) azurin. A comparison of S K-edge XAS of WT and M121SeM azurin an...
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PMID: 18314977
PDF is available here.
PDF is available here.
Abstract
Biochemistry (Washington) (47)11 2008
Abstract
This study reports the first quantitative estimate of the thermodynamic stability (Delta G degrees ) of a protein in low-temperature partly frozen aqueous solutions in the presence of the protective osmolytes trimethylamine N-oxide (TMAO), glycine betaine, and sarcosine. The method, based on guanidi...
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PMID: 18293933
PDF is available here.
PDF is available here.
Abstract
We report that azurin and an azurin-like protein (Laz) from gonococci/meningococci have activity against Toxoplasma, an apicomplexan parasite that causes opportunistic infection in immunocompromised individuals. We demonstrate that the mechanism of action for Laz involves interfering with the abilit...
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PMID: 18070964
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)5 2008
Abstract
Copper-containing nitrite reductase is able to catalyze the reduction of nitrite with a turnover rate of several hundreds per second. Electrons for the reaction are donated by the electron transfer protein pseudoazurin. The process of protein complex formation, electron transfer and dissociation mus...
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PMID: 18083191
PDF is available here.
PDF is available here.
Natural Product Reports (25)1 2008
Abstract
Many approaches are being used to engineer metalloproteins, with most of these informed by, and aiming to further elucidate, the basic structural requirements for biological metal centers. Cupredoxins are type 1 (T1) copper-containing electron transfer (ET) proteins with a -barrel fold that is thoug...
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PMID: 18250895
PDF is available here.
PDF is available here.
Methods in Enzymology (437) 2008
Abstract
The two subunit cytochrome bc complex (NorBC) isolated from membranes of the model denitrifying soil bacterium Paracoccus denitrificans is the best characterized example of the bacterial respiratory nitric oxide reductases. These are members of the superfamily of heme-copper oxidases and are charact...
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PMID: 18433624
PDF is available here.
PDF is available here.
Abstract
A quartz crystal microbalance coupled with electrochemistry was used to examine the adsorption of azurin on a gold electrode modified with a self-assembled monolayer of octanethiol. Azurin adsorbed irreversibly to form a densely packed monolayer. The rate of azurin adsorption was related to the bulk...
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PMID: 18041855
PDF is available here.
PDF is available here.
Journal of Inorganic Biochemistry (102)5-6 2008
Abstract
Several blue copper proteins are known to change the active site structure at alkaline pH (alkaline transition). Spectroscopic studies of Met16Phe, Met16Tyr, Met16Trp, and Met16Val pseudoazurin variants were performed to investigate the second sphere role through alkaline transition. The visible ele...
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PMID: 18343503
PDF is available here.
PDF is available here.
Abstract
The 'immunoglobulin-like' fold is one of most common structural motifs observed in proteins. This topology is found in more than 80 superfamilies of proteins, including Cu,Zn-superoxide dismutase (SOD) and cupredoxin. Evolutionary relationships have not been identified, but may exist. The challenge...
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PMID: 18080994
PDF is available here.
PDF is available here.
Abstract
We get insight into the kinetics of this complex formation, by exploring the interaction between p53 and azurin in their environment by single molecule force spectroscopy. To this aim, azurin has been linked to the atomic force microscope tip, whereas p53 has been immobilized onto a gold substrate....
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PMID: 18247358
PDF is available here.
PDF is available here.
Abstract
The long 15-residue type 1 copper-binding loop of nitrite reductase has been replaced with that from the cupredoxin amicyanin (7 residues). This sizable loop contraction does not have a significant effect on the spectroscopy, and therefore, the structures of both the type 1 and type 2 Cu(II) sites....
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PMID: 18491346
PDF is available here.
PDF is available here.
ChemBioChem (8)16 2007
Abstract
The role played by the alpha-helix in determining the structure, the stability and the unfolding mechanism of azurin was addressed by studying a helix-depleted azurin variant produced by site-directed mutagenesis. The protein structure was investigated by CD, 1D (1)H NMR, fluorescence spectroscopy m...
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PMID: 17868155
PDF is available here.
PDF is available here.
Abstract
We have explored the effects of the cofactors on CopC unfolding in vitro. We demonstrate that Cu(II) coordination affects both protein stability and unfolding pathway, whereas Cu(I) has only a small effect on stability. Apo-CopC unfolds in a two-state reaction between pH 4 and 7.5 with maximal stabi...
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PMID: 17889826
PDF is available here.
PDF is available here.
Abstract
Abstract
Rusticyanin (RCy) mediated transfer of electron to Cytochrome C(4) (Cytc(4)) from the extracellular Fe(+2) ion is primarily involved in the Thiobacillus ferrooxidans induced bio-leaching of pyrite ore and also in the metabolism of this acidophilic bacteria. The modeling studies have revealed the two...
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PMID: 17718594
PDF is available here.
PDF is available here.
Biochemistry (Washington) (46)35 2007
Abstract
Atomic resolution structures of the pseudoazurin (PAZ) variant into which the shorter ligand-containing loop of amicyanin (AMI) is introduced have been determined. The mutated loop adopts a different conformation in PAZAMI than in AMI. The copper site structure is affected, with the...
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PMID: 17685636
PDF is available here.
PDF is available here.