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Bacteriocins
Abstract
These results show that wild animals constitute an appropriate source for the isolation of bacteriocinogenic enterococci....
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PMID: 21819651
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PDF is available here.
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Ceska a Slovenska farmacie : casopis Ceske farmaceuticke spolecnosti a Slovenske farmaceuticke spolecnosti (60)2 2011
Abstract
Lactic acid bacteria comprise several genera of gram-positive bacteria that are known for the production of structurally different antimicrobial substances. Among them, bacteriocins are nowadays in the centre of scientific interest. Bacteriocins, proteinaceous antimicrobial substances, are produced r...
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PMID: 21650008
PDF is available here.
PDF is available here.
Abstract
In the present study, the bacteriocins produced by Staphylococcus aureus 4185, a strain isolated from bovine mastitis, were purified and partially characterized. After purification by ammonium sulfate precipitation, cation-exchange chromatography, and five runs of high-performance liquid chromatogra...
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PMID: 20618078
PDF is available here.
PDF is available here.
Journal of Dairy Science (93)10 2010
Abstract
I and II using Fourier transform infrared spectroscopy yielded a considerable species diversity, with lactic acid bacteria increasing strongly during the 8-h cultivation. Therefore, 23 lactic acid bacteria bacteriocin genes were assayed using specific PCR primers, identifying 3 bacteriocin genes in...
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PMID: 20854983
PDF is available here.
PDF is available here.
Abstract
The recombinant DNA pLR5cat_PSAB in which pediocin PA-1 structural and immunity genes (pedAB) fused with the promoter and deduced signal sequence of an alpha-amylase gene from a bifidobacterial strain were inserted in pLR5cat, an Escherichia coli-lactobacilli shuttle vector was transferred to Lactob...
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PMID: 20798585
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (59)Pt 8 2010
Abstract
Antagonistic and synergistic substances are important for interactions between micro-organisms associated with human body surfaces, either in healthy or in diseased conditions. In the present study, such compounds produced by Gardnerella vaginalis strains isolated from women with bacterial vaginosis...
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PMID: 20466841
PDF is available here.
PDF is available here.
Journal of Dairy Science (93)8 2010
Abstract
Lantibiotics are small (<5 kDa), polycyclic peptides produced by gram-positive bacteria; they are also known as gram-positive bacteriocins. The high antimicrobial activity of lacticins and the continuing appearance of antibiotic-resistant bacteria in recent years have resulted in a renewed interest...
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PMID: 20655430
PDF is available here.
PDF is available here.
Abstract
The microflora of four batches of traditional Greek Graviera cheese was studied at 5 weeks of ripening, and 200 lactic acid bacteria (LAB) isolates were phenotypically characterized and screened for antilisterial bacteriocins. The cheeses were also analyzed for organic acids by high-performance liqu...
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PMID: 20615342
PDF is available here.
PDF is available here.
Journal of Antibiotics (63)7 2010
Abstract
Deoxyactagardine B (DAB) is a hitherto unknown type B lantibiotic, produced by Actinoplanes liguriae NCIMB41362. The mature peptide is 19 amino acids in length and structurally analogous to actagardine, differing by two amino acids (V15L and I16V) and the absence of a sulfoxide bond between residues...
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PMID: 20520597
PDF is available here.
PDF is available here.
Abstract
The bacteriocins produced by Lactococcus lactis subsp. lactis C101910 (C101910) and NBRC 12007 (NBRC 12007) were used to prevent the growth of sake spoiling hiochi bacteria (Lactobacillus hilgardii, Lactobacillus fructivorans, and Lactobacillus paracasei) in namazake, which is raw (unpasteurized) sa...
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PMID: 20471596
PDF is available here.
PDF is available here.
Microbiology (156)Pt 6 2010
Abstract
The importance of 3D structuring in the N- and C-terminal ends of the two peptides (39-mer LcnG-alpha and 35-mer LcnG-beta) that constitute the two-peptide bacteriocin lactococcin G was analysed by replacing residues in the end regions with the corresponding D-isomeric residues. When assayed for ant...
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PMID: 20203056
PDF is available here.
PDF is available here.
Abstract
We have identified and purified thuricin CD, a two-component antimicrobial that shows activity against C. difficile in the nanomolar range. Thuricin CD is produced by Bacillus thuringiensis DPC 6431, a bacterial strain isolated from a human fecal sample, and it consists of two distinct peptides, Trn...
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PMID: 20435915
PDF is available here.
PDF is available here.
Abstract
On most dairy farms teat dips are applied to the teats of cows either before or after milking in order to prevent pathogens from gaining access to the mammary gland via the teat canal. In the present experiments, a natural teat dip was developed using a fermentate containing the live bacterium Lacto...
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PMID: 19785910
PDF is available here.
PDF is available here.
Meat Science (84)4 2010
Abstract
These results indicate that AS-48 can be applied alone to control L. monocytogenes and combined with HHP treatment to control Salmonella in fuets.
2009. Published by Elsevier Ltd....
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PMID: 20374829
PDF is available here.
PDF is available here.
Abstract
A finished compost sample was examined for bacteriocin-like substance production against five pathogenic bacteria: Salmonella typhimurium EF 85-9, Escherichia coli O157:H7 ATCC 43888, Enterococcus faecalis JCM 8726, Staphylococcus aureus JCM 2151, and Yersinia enterocolitica JCM 7577. At the prelimi...
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PMID: 20513963
PDF is available here.
PDF is available here.
Journal of Biotechnology (145)4 2010
Abstract
Aiming to facilitate the accessibility of recombinant proteins produced with Escherichia coli, extracellular expression may be achieved by means of bacteriocin release protein (BRP) coexpression. Different types of BRPs were tested in order to optimize protein secretion into the culture medium. Thos...
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PMID: 19958803
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
The anti-Pr CA and rabbit anti-human GPA increased exposure of PE and increased membrane Ca(2+) permeability that may have caused hemolysis. The difference in the responses to these antibodies to enzyme treatment of RBCs suggests that they react with different epitopes on GPA....
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PMID: 19843291
PDF is available here.
PDF is available here.
Abstract
Different methods were used to elucidate the mode of action of thuricin S, a new class IId bacteriocin produced by Bacillus thuringiensis subsp. entomocidus HD198. According to cell viability tests, thuricin S was shown to exert a bactericidal effect on the sensitive cells of Bacillus thuringiensis...
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PMID: 20237578
PDF is available here.
PDF is available here.
Abstract
Lactococcin G and enterocin 1071 are two homologous two-peptide bacteriocins. Expression vectors containing the gene encoding the putative lactococcin G immunity protein (lagC) or the gene encoding the enterocin 1071 immunity protein (entI) were constructed and introduced into strains sensitive to o...
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PMID: 20038710
PDF is available here.
PDF is available here.
Abstract
We sought to characterize 128 isolates from the sputum of adults with CF, along with 45 isolates from patients with invasive diseases for comparison. The tests performed included Lancefield grouping; tests for hemolysis; tests for the production of hyaluronidase, chondroitin sulfatase, DNase, protea...
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PMID: 20007382
PDF is available here.
PDF is available here.
Abstract
The aim of the present work was to study the mode of the induction of the biosynthesis of macedocin, the lantibiotic produced by Streptococcus macedonicus ACA-DC 198. Macedocin was produced when the strain was grown in milk but not in MRS or M17 broth. No autoinduction mechanism was observed. Produc...
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PMID: 20023075
PDF is available here.
PDF is available here.
Abstract
The technological feasibility of producing fermented sausages using the bacteriocin-producing Lactobacillus pentosus 31-1, isolated from a traditional Chinese fermented meat product (Xuanwei ham), was evaluated. Strain 31-1 was used both as a single starter and in coculture for manufacture of fermen...
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PMID: 20132674
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)4 2010
Abstract
We establish for the first time, through a combination of reverse genetics, mass spectrometry, and mutagenesis, that these genes encode a functional lantibiotic. We also reveal that Bsa is identical to the previously identified bacteriocin staphylococcin Au-26, produced by an S. aureus strain of vag...
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PMID: 20023032
PDF is available here.
PDF is available here.
Science progress (93)Pt 1 2010
Abstract
The complex physical and chemical conditions encountered in the gut present a range of physiological challenges to both the commensal microbiota and to pathogenic microorganisms attempting to colonise the gut. The innate immune system of the host, the host's diet and the microbial population present...
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PMID: 20222354
PDF is available here.
PDF is available here.
Abstract
We will focus on a well-characterized form of biochemical warfare: bacteriocins produced by Streptococcus mutans, a primary dental caries pathogen, and H(2)O(2) produced by Streptococcus sanguinis, an oral commensal. We will describe detailed methodologies on the competition assay, isolation, purifi...
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PMID: 20717784
PDF is available here.
PDF is available here.
Abstract
A method of separation and purification of macromolecular bacteriocins of phytopathogenic bacterium Erwinia carotovora is proposed. It is shown on the basis of polypeptide composition of the particles and their killer specificity, that E. carotovora ESP86 is a complex defective-polylysogenic system...
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PMID: 21117296
PDF is available here.
PDF is available here.
Abstract
Present study was carried for the isolation of bacteriocin producing Enterococci from indigenous sources. Gram positive enterococci are known for having the ability to produce enterocins with good antimicrobial potential. A total of 34 strains were isolated from processed dairy products of Pakistan...
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PMID: 20134247
PDF is available here.
PDF is available here.
Voprosy Virusologii (55)4 2010
Abstract
Light and immunofluorescence microscopies were used to study the cytopathic effect of herpes simplex virus type 1 (HSV-1) grown on the Vero cell cultures in the absence or presence of supernatants of Enterococcus faecium L3, Lactobacillus plantarum 8A-P3, and Escherichia coil M17. The effect of the...
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PMID: 20886709
PDF is available here.
PDF is available here.
Mikrobiologiia (79)1 2010
Abstract
Abstract
Recombinant divercin RV41 (DvnRV41) and its structural variants were used in this study to assess their antilisterial activities in vivo in mice challenged intravenously with Listeria monocytogenes EGDe. Treatment with DvnRV41 before and after infection permitted a conclusion as to the capacities of...
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PMID: 19841145
PDF is available here.
PDF is available here.
Abstract
We investigated effects of negatively charged lipids on the binding and pore formation of lacticin Q with liposomes by surface plasmon resonance analysis and fluorescence dye leakage experiments respectively. Negatively charged lipids accelerated the binding of lacticin Q on the membranes and the re...
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PMID: 20057115
PDF is available here.
PDF is available here.
BMC Microbiology (10) 2010
Abstract
Mutants of E. faecalis V583 resistant to pediocin PA-1 were isolated, and their gene expression profiles were analyzed and compared to the wild type using whole-genome microarray. Significantly altered transcription was detected from about 200 genes; most of them encoding proteins involved in energy...
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PMID: 20738841
PDF is available here.
PDF is available here.
Abstract
Enterococci are among the most common human intestinal lactic acid bacteria, and they are known to produce bacteriocins. In this study, fecal enterococci were isolated from infants and screened for bacteriocin production. Bacteriocin-producing Enterococcus avium isolates were obtained, and a new ped...
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PMID: 19933345
PDF is available here.
PDF is available here.
Abstract
The cyclic peptide bacteriocin enterocin AS-48 was tested (at final concentrations of 0.175, 0.613, and 1.05 AU/ml) against the exopolysaccharide-producing cider spoilage strain Pediococcus parvulus 48 in apple juice in combination with high-intensity pulsed electric field (HIPEF) treatment (35 kV/c...
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PMID: 20051202
PDF is available here.
PDF is available here.
Abstract
We designated it E. faecium IJ-31. The precise identity of this strain was then established by determining the sequence of its 16S and 23S rRNA genes. The sequence homology searches revealed matches with a number of previously reported strains, such as E. faecium HN-N3 and HN-N29, both isolated from...
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PMID: 20051203
PDF is available here.
PDF is available here.
Abstract
To demonstrate treatment efficacy of bacteriocin S760 synthesized by Enterococcus faecium 760 for septic Salmonella infection in mice.
One hundred mice, which were intraperitoneally inoculated with 100 LD50 of Salmonella enteritidis strain 92 Rif(r), received bacteriocin 1 hour (prevention) or 48 ho...
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PMID: 21061577
PDF is available here.
PDF is available here.
Abstract
A bacteriocin-producing strain PI80 was isolated from the gut of Penaeus indicus (Indian white shrimp) and identified as Streptococcus phocae PI80. The bacteriocin was purified from a culture supernatant to homogeneity as confirmed by Tricine SDS-PAGE. Reverse-phase HPLC analysis revealed a single a...
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PMID: 19996693
PDF is available here.
PDF is available here.
Abstract
The peptide antibiotic mutacin 1140 belongs to the group of antibiotics called lantibiotics. They are ribosomally synthesized and undergo extensive enzymatic modifications before being excreted into the culture medium. By using reverse-phase-high-performance liquid chromatography (RP-HPLC) and a sem...
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PMID: 19940934
PDF is available here.
PDF is available here.
Abstract
For several years in the Quebec fisheries' industry, landings of pelagic fish have been calculated at over 4000 tons. These under-exploited species, rich in lipids and proteins, could be used in valuable new products. In the present study, hydrolysates of mackerel and herring were produced and utili...
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PMID: 19940932
PDF is available here.
PDF is available here.
Abstract
Abstract
Competition assays with Sinorhizobium meliloti 1021 and its GFP-labelled pSymA cured and deleted derivatives, SmA818 and SmA146, demonstrated that Sm1021 could still inhibit rice seedling growth even when outnumbered by a large excess of the noninhibitory cured or deleted strain. The wild-type strai...
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PMID: 19935894
PDF is available here.
PDF is available here.
Abstract
Lantibiotics are ribosomally synthesized and post-translationally modified peptide antibiotics containing the characteristic thioether cross-links lanthionine and methyllanthionine. To date, no analogues of lantibiotics that contain nonproteinogenic amino acids have been reported. In this study, in...
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PMID: 19655738
PDF is available here.
PDF is available here.
Abstract
Members of the Gram-positive bacterial genus Streptococcus are a diverse collection of species inhabiting many body sites and range from benign, nonpathogenic species to those causing life-threatening infections. The streptococci are also prolific producers of bacteriocins, which are ribosomally syn...
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PMID: 19722837
PDF is available here.
PDF is available here.
Food Microbiology (26)6 2009
Abstract
In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 degrees...
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PMID: 19527838
PDF is available here.
PDF is available here.
Food Microbiology (26)6 2009
Abstract
In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 degrees...
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PMID: 19527838
PDF is available here.
PDF is available here.
Food Microbiology (26)6 2009
Abstract
In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 degrees...
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PMID: 19527838
PDF is available here.
PDF is available here.
Food Microbiology (26)6 2009
Abstract
In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 degrees...
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PMID: 19527838
PDF is available here.
PDF is available here.
Food Microbiology (26)6 2009
Abstract
In realistic model meat systems, the separate and combined effects of fat content and sodium nitrite on the antilisterial activity of the bacteriocin of Lactobacillus curvatus CWBI-B28 were studied. In laboratory fermentations where Listeria monocytogenes was co-cultured at 4 degrees...
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PMID: 19527838
PDF is available here.
PDF is available here.
Abstract
The three-dimensional structures of the two peptides, PlnJ and PlnK, that constitutes the two-peptide bacteriocin plantaricin JK have been solved in water/TFE and water/DPC-micellar solutions using nuclear magnetic resonance (NMR) spectroscopy. PlnJ, a 25 residue peptide, has an N-terminal amphiphil...
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PMID: 19538999
PDF is available here.
PDF is available here.
Journal of Organic Chemistry (74)15 2009
Abstract
A regiocontrolled intermolecular Diels-Alder reaction of an o-benzoquinone followed by an intramolecular nitrile oxide cyclization is employed to prepare the BCD fragment of viridin. The AE segment is attached to it by means of an intramolecular Diels-Alder reaction of an o-benzoquinone monoketal ge...
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PMID: 19572524
PDF is available here.
PDF is available here.
Abstract
Bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from Lactobacillus brevis (a safe food-grade bacteria isolated from Vari Kandal, a traditional fermented food of Himachal Pradesh by adopting a novel repeated washing method. Its purity was confirmed by SDS-PA...
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PMID: 19788067
PDF is available here.
PDF is available here.
Journal of Chemotherapy (21)4 2009
Abstract
Probiotics are gaining increasing scientific and commercial interest as functional foods. Their success has led to the development and marketing of a broad range of products based on probiotics. In this context, resolution of the taxonomy of microbial species remains a key point, since different spe...
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PMID: 19622453
PDF is available here.
PDF is available here.
Abstract
Enterocin AS-48 was tested against Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes in different kinds of desserts. The highest activity against S. aureus was detected in baker cream. However, in yogurt-type soy-based desserts and in gelatin pudding, AS-48 (175 arbitrary units [AU]...
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PMID: 19722396
PDF is available here.
PDF is available here.
Abstract
The aim of this research work was to identify and characterized the bacteriocins produced by soil-associated bacteria. Bacillocin from Bacillus brevis Bb and pyocin from Pseudomonas aeruginosa Pa were found bioactive only against gram-positive bacteria tested. Maximum production of both the bacterio...
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PMID: 19553169
PDF is available here.
PDF is available here.
Abstract
Apoptosis (programmed cell death) and necrosis (uncontrolled cell death) are two distinct processes of cell death that have been described. Non-invasive molecular imaging of these two processes can have several clinical applications and has various approaches in pre-clinical research...
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PMID: 19362149
PDF is available here.
PDF is available here.
Chemistry & Biology (16)5 2009
Abstract
Mersacidin is a tetracyclic lantibiotic with antibacterial activity against Gram-positive pathogens. To probe the specificity of the biosynthetic pathway of mersacidin and obtain analogs with improved antibacterial activity, an efficient system for generating variants of this lantibi...
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PMID: 19477413
PDF is available here.
PDF is available here.
Abstract
Apoptosis (programmed cell death) and necrosis (uncontrolled cell death) are two distinct processes of cell death that have been described. Non-invasive molecular imaging of these two processes can have several clinical applications and has various approaches in pre-clinical research...
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PMID: 19362149
PDF is available here.
PDF is available here.
Chemistry & Biology (16)5 2009
Abstract
Mersacidin is a tetracyclic lantibiotic with antibacterial activity against Gram-positive pathogens. To probe the specificity of the biosynthetic pathway of mersacidin and obtain analogs with improved antibacterial activity, an efficient system for generating variants of this lantibi...
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PMID: 19477413
PDF is available here.
PDF is available here.
Chemistry & Biology (16)5 2009
Abstract
Mersacidin is a tetracyclic lantibiotic with antibacterial activity against Gram-positive pathogens. To probe the specificity of the biosynthetic pathway of mersacidin and obtain analogs with improved antibacterial activity, an efficient system for generating variants of this lantibi...
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PMID: 19477413
PDF is available here.
PDF is available here.
Chemistry & Biology (16)5 2009
Abstract
Mersacidin is a tetracyclic lantibiotic with antibacterial activity against Gram-positive pathogens. To probe the specificity of the biosynthetic pathway of mersacidin and obtain analogs with improved antibacterial activity, an efficient system for generating variants of this lantibi...
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PMID: 19477413
PDF is available here.
PDF is available here.