Abstract
To study the impact of coagulase-negative staphylococcal (CNS) primary and intravascular catheter-related bloodstream infection (PBSI/CRBSI) on mortality and morbidity in critically-ill patients.
|
PMID: 21130534
PDF is available here.
Abstract
The activity of telavancin was evaluated against Staphylococcus spp. collected from European hospitals as part of an international surveillance study (2007-2008). A total of 7534 staphylococcal clinical isolates [5726 Staphylococcus aureus and 1808 coagulase-negative staphylococci (CoNS)] were inclu...
|
PMID: 20598860
PDF is available here.
Abstract
To determine the in vitro susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative Staphylococcus (MRCNS) isolates to various antibiotics.
All cases of bacterial keratitis caused by Staphylococcus species during 2006 and 2007 were identified....
|
PMID: 20595899
PDF is available here.
Abstract
Gram-positive bacteria such as Staphylococcus aureus have been a common cause of infection among liver transplant (LT) recipients in recent decades. The understanding of local epidemiology and its evolving trends with regard to pathogenic spectra and antibiotic susceptibility is beneficial to prophy...
|
PMID: 20819529
PDF is available here.
François Barbier,
Etienne Ruppé,
David Hernandez,
David Lebeaux,
Patrice Francois,
Benjamin Felix,
Adeline Desprez,
Aminata Maiga,
Paul-Louis Woerther,
Kevin Gaillard,
Cécile Jeanrot,
Michel Wolff,
Jacques Schrenzel,
Antoine Andremont and
Raymond Ruimy
Abstract
MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRSA....
|
PMID: 20550456
PDF is available here.
Abstract
Staphylococcus spp. are microorganisms that are naturally present in milk and dairy products and are often associated with food-borne diseases outbreaks due to the ability of some strains to produce thermostable enterotoxins. This ability is usually associated with coagulase and thermonuclease produ...
|
PMID: 20417392
PDF is available here.
Abstract
We investigated the relationship between the time from obtaining the blood specimen to the detection of positive culture tests as well as the significance of the pathogen detected. Twenty-three inpatients demonstrated CNS-positive blood culture tests. It was judged that six patients (26%) had contam...
|
PMID: 20622485
PDF is available here.
Abstract
In recent years, coagulase-negative staphylococci (CoNS) have been increasingly recognised as causative agents of various infections, especially in immunocompromised patients and related to implanted foreign body materials. CoNS, and especially Staphylococcus epidermidis, transform into a stationary...
|
PMID: 20221891
PDF is available here.
Abstract
We tested the immunogenicity of a peptide subunit vaccine coding for three different adhesion factors, fibrinogen-binding protein (Efb), fibronectin-binding protein A (FnbpA) and clumping factor A (ClfA). Then we evaluated the influence of some virulence factors on the ability of specific anti-adhes...
|
PMID: 20030902
PDF is available here.
Abstract
We used clumping factor A (clfA) to profile 87 bovine mastitis isolates from four regions in Canada and compared the results to those obtained by pulsed-field gel electrophoresis (PFGE) and spa typing. Twenty-five pulsotypes were obtained by PFGE with an index of discrimination of 0.91. These were a...
|
PMID: 19955267
PDF is available here.
Abstract
The FISH assay showed 91.8% sensitivity and 100% specificity for S. aureus and 100% sensitivity and 93.3% specificity for CoNS when hybridized with STA and SAU probes. Meanwhile, the assay showed 91.8% sensitivity and 100% specificity for S. aureus and 100% sensitivity and 90% specificity for CoNS,...
|
PMID: 20357507
PDF is available here.
Abstract
Human plasma was more sensitive than sheep plasma for the tube coagulase test (sensitivity of 91% vs. 81% respectively), but both plasmas had very low specificity (11% and 7% respectively). The sensitivity and specificity of the tube coagulase test (human plasma) was markedly improved when Mannitol...
|
PMID: 20707914
PDF is available here.
Abstract
A set of degenerate PCR primers was designed and used to amplify and sequence about 75% of the catalase (kat) gene from each of 49 staphylococcal strains. In some strains of Staphylococcus xylosus, S. saprophyticus, and S. equorum, two catalase genes, katA and katB, were found. A phylogenetic tree w...
|
PMID: 19889901
PDF is available here.
Abstract
Staphylococcus aureus is one of the most important etiologic agents of ovine mastitis. To develop effective control measures for mastitis, it is important to type S. aureus strains that have considerable genetic heterogeneity. In the current study, 47 S. aureus strains isolated from ovine mastitis w...
|
PMID: 19901288
PDF is available here.
Abstract
Staphylococcus aureus colonization was identified in 67/242 (28%) humans, 19/132 (14%) dogs, and 7/161 (4.3%) cats in households in Ontario, with methicillin-resistant S. aureus present in 8 (3.3%) humans, 2 (1.5%) dogs, and 0 cats. Staphylococcus pseudintermedius was isolated from 8 (4.1%) humans,...
|
PMID: 19949556
PDF is available here.
Abstract
Endophthalmitis following intravitreal injection is an infrequent but serious clinical problem. Infectious endophthalmitis must be differentiated from noninfectious inflammation. Depending on the series, the risk of endophthalmitis is very low. The use of a standardized injection protocol can reduce...
|
PMID: 19586422
PDF is available here.
Abstract
All 4 patients had keratoprosthesis surgery due to previous corneal transplant failure. The incidence of endophthalmitis was 11.4%. All patients were on topical antibiotic prophylaxis with a fluoroquinolone and 1 of the 4 patients was also using vancomycin drops. All 4 patients underwent a tap and i...
|
PMID: 19654529
PDF is available here.
Abstract
A fetal inflammatory response is protective for chronic lung disease. Neonatal sepsis is strongly associated with chronic lung disease, and the infecting organism is important. Coagulase-negative staphylococcal infection confers a risk for chronic lung disease similar to that of other bacteremias. C...
|
PMID: 19403497
PDF is available here.
Abstract
Seventy-eight staphylococcal strains were isolated from surgical-site, blood-stream and other hospital-acquired infections. Eighteen isolates were determined as methicillin (MET)-resistant S. aureus (MRSA), while the remaining were MET-resistant coagulase-negative staphylococci (CoNS). Fifty percent...
|
PMID: 19418254
PDF is available here.
Author(s) unavailable
Abstract
Coagulase-negative staphylococci (CNS) are differentiated from the closely related but more virulent Staphylococcus aureus by their inability to produce free coagulase. Currently, there are over 40 recognized species of CNS. These organisms typically reside on healthy human skin and mucus membranes,...
|
PMID: 19135917
PDF is available here.
Abstract
These results were compared with the results obtained by using the detection kit SET-RPLA for the specific detection of staphylococcal enterotoxins (SEA-SED).
Eighty-one samples of milk and milk products were analyzed for the presence of Staphylococcus strains. Forty-six coagulase po...
|
PMID: 19759471
PDF is available here.
Abstract
Our data show that the R-domain can be useful for typing and grouping host-specific lineages. Moreover, existence of variant repeats in human strains and the dominance of a clonal motif in mastitis may imply that a specific selection has occurred in the mammary gland....
|
PMID: 18990534
PDF is available here.
Abstract
Overall agreement was 96.7%. Sensitivity for S. aureus was 96.5% (83/86). Specificity for S. aureus was 100% (215/215). Sensitivity for CNS was 96.6% (201/208). Specificity for CNS was 96.8% (90/93). Three cultures reported as S. aureus were identified as CNS by S. aureus/CNS PNA FISH. Three culture...
|
PMID: 19354026
PDF is available here.
Abstract
Conjunctival bacterial flora among newborns in NICU is varied, mainly Gram-positive, usually multiresistant to antibiotics....
|
PMID: 19668955
PDF is available here.
Abstract
We developed a novel staphylocoagulase serotyping method based on a microplate format using polystyrene latex particles. Addition of latex particles promotes the formation of fibrin complexes, which represents a more rapidly and easily detected endpoint. For 83 strains, 90% were classified into sero...
|
PMID: 20528093
PDF is available here.
Abstract
Coagulase-negative Staphylococcus, Staphylococcus aureus, and Corynebacterium species were the most commonly isolated bacterial species in the PD group. Among all bacterial isolates, only Staphylococcus aureus was significantly higher in the conjunctival flora of patients with PD than in those in th...
|
PMID: 19373572
PDF is available here.
Abstract
This study investigated slime production by coagulase-negative staphylococci (CNS) using the standard tube (ST), Congo red agar (CRA) plate and Christensen's tube (CT) methods, and compared the results with those of the crystal violet reaction (CVR) test. The potential correlation between slime prod...
|
PMID: 19215681
PDF is available here.
Abstract
This study investigated the colonization of slime-producing coagulase-negative Staphylococcus (CoNS) in 80 patient wards in Turkey (40 vinyl and 40 ceramic tile floors). A total of 480 samples that included 557 CoNS isolates were obtained. Slime production was investigated with the Christensen metho...
|
PMID: 19589249
PDF is available here.
Abstract
Many identification and relatedness studies methods had been commonly used for epidemiological studies in microbiological laboratories. Apart from phenotypic methods, genotypic are also often used. The aim of this study was to compare, obtained by PFGE chromosomal DNA patterns of methicillin-resista...
|
PMID: 19780488
PDF is available here.
Abstract
Staphylococcus spp. are a frequent cause of ABM in hospitals with neurosurgery, especially in the post-operative period following neurosurgical procedures and/or in carriers of CSF drainage devices. Prognosis in infections caused by CoN Staphylococci is favourable, in contrast to the high mortality...
|
PMID: 19145558
PDF is available here.
Abstract
11 of 19 (58 %) catheter isolates and 14 of 47 (30 %) skin isolates (p = 0.04) produced biofilm. We found an increasing prevalence of oxacillin resistance (20 % versus 67 %, p = 0.004) and gentamicin resistance (15 % versus 67 %, p = 0.003) after six months of cancer treatment. Biofilm positive CoNS...
|
PMID: 19079425
PDF is available here.
Abstract
We passively immunized lactating mice with rabbit antibodies to S. aureus capsular polysaccharide (CP) serotype 5 (CP5) or CP8 or with monoclonal antibodies to ClfA. Mice immunized with antibodies to CP5 or CP8 or with ClfA had significantly reduced tissue bacterial burdens 4 days after intramammary...
|
PMID: 18809660
PDF is available here.
Abstract
In all but seven MRSA isolates, MICs were below the concentrations achievable experimentally at application sites suggesting therapeutic efficacy of both antibiotics in infections involving multiresistant staphylococci and for decolonization of carriers. However, the seven MRSA with high MICs, all o...
|
PMID: 18819974
PDF is available here.
Abstract
Isolates of various species of coagulase-negative staphylococci (CNS) from extramammary swab samples were compared with isolates of bovine mastitis CNS species. Swab samples were taken from perineum skin and udder skin, teat apices and teat canals of lactating dairy cows of the research dairy herd o...
|
PMID: 18700996
PDF is available here.
Abstract
Coagulase gene restriction fragment length polymorphism (RFLP), six-locus variable-number tandem-repeat analysis patterns (MLVA) and detection of enterotoxin genes (se) (sea, sec, sed, seg, seh, sei, sej and sel) were used to determine the phylogenetic relationship among isolates of Staphylococcus a...
|
PMID: 18701004
PDF is available here.
Abstract
In this study a comprehensive analysis of toxin production of food associated coagulase-negative staphylococci (CNS) was investigated. The strains belong to the following staphylococcal species, Staphylococcus carnosus, Staphylococcus condimenti, Staphylococcus equorum, Staphylococcus piscifermentan...
|
PMID: 18752861
PDF is available here.
Abstract
We show here that surface proteins of Staphylococcus aureus arrive at two distinct destinations in the bacterial envelope, either distributed as a ring surrounding each cell or as discrete assembly sites. Proteins with ring-like distribution (clumping factor A (ClfA), Spa, fibronectin-binding protei...
|
PMID: 18800056
PDF is available here.
Abstract
We immunized mice with rAls3p-N or negative control proteins and challenged them via the tail vein with S. aureus or other gram-positive or gram-negative pathogens. The rAls3p-N vaccine, but neither tetanus toxoid nor a related Als protein (Als5p), improved the survival of vaccinated mice subsequent...
|
PMID: 18644876
PDF is available here.
Abstract
Ten hand-touch sites were screened weekly on two surgical wards over two consecutive six-month periods. The results were analysed using hygiene standards, which specify (i) an aerobic colony count (ACC) > 2.5 cfu/cm
2, and (ii) presence of coagulase-positive staphylococci, as hygiene...
|
PMID: 18821374
PDF is available here.
Abstract
There are 104 DHF patients enrolled in the study; 57 are assigned in dextran and 47 in haes-steril group. The mean ages are 8.6 +/- 3.9 years. About half of the patients in both groups require one dose of colloidal solution and 25% require 2 and 3 doses (p = 0.138). The average amount of IV fluid in...
|
PMID: 19253503
PDF is available here.
Abstract
The resistance of 330 coagulase-negative staphylococci (CNS) associated with food or used in starter cultures and belonging to the species Staphylococcus carnosus, Staphylococcus condimenti, Staphylococcus piscifermentans, Staphylococcus equorum, Staphylococcus succinus and Staphylococcus xylosus, a...
|
PMID: 18625535
PDF is available here.
Abstract
In the present study the role of different mammalian cell receptors in adherence of the coagulase positive pathogen, Staphylococcus aureus and some coagulase negative staphylococci, namely Staphylococcus epidermidis and Staphylococcus saprophyticus was investigated. Upon testing the adherence to Ver...
|
PMID: 18702074
PDF is available here.
Abstract
We report a new convenient method for fibrinogen purification based on the affinity of Staphylococcus aureus clumping factor A to fibrinogen. Clumping factor A (ClfA) is a cell wall-anchored surface protein of S. aureus bacteria that binds with a high affinity to the fibrinogen gamma chain C-terminu...
|
PMID: 18558494
PDF is available here.
Sadia Afroz,
Nobumichi Kobayashi,
Shigeo Nagashima,
M Mahbub Alam,
A B M Bayezid Hossain,
M Abdur Rahman,
M Rafiqul Islam,
Afzalunnessa Binte Lutfor,
Naima Muazzam,
M Abul Hossain Khan,
Shyamal Kumar Paul,
A K M Shamsuzzaman,
M Chan Mahmud,
A K M Musa and
M Akram Hossain
Abstract
We investigated 59 strains (49 isolates from clinical specimens and 10 isolates colonized in the nasal cavities of medical staff), including 26 methicillin-resistant S. aureus (MRSA) strains. The PVL gene was detected only in methicillin-susceptible S. aureus (MSSA) strains (7 clinical strains and 2...
|
PMID: 18806351
PDF is available here.
Abstract
We reported our experience with the usefulness and interpretation of the significance of cultures at the second-stage exchange of infected arthroplasty in a prospective, 3-year follow-up study. When such intraoperative cultures were negative, patients received no therapy; when at least two cultures...
|
PMID: 18431606
PDF is available here.
Abstract
Neonatal sepsis causes significant mortality and morbidity. Coagulase-negative staphylococci (CoNS) and Candida frequently cause neonatal sepsis at >72 h of age. Lactoferrin, which is present in human milk, is a component of innate immunity and has broad-spectrum antimicrobial activity. The synergis...
|
PMID: 18719181
PDF is available here.
Abstract
The genus Staphylococcus is made up of 36 validated species which contain strains that are pathogenic, saprophytic, or used as starter cultures for the food industry. Staphylococci species used in cheese-making are novobiocin-resistant, coagulase-negative and are not usually identified at species le...
|
PMID: 17869361
PDF is available here.
Abstract
Fast, sensitive and cheap determination of pathogenic bacteria is extremely important in many branches, for example biotechnology, quality control, analysis of samples and antimicrobial therapy. The development and application of analytical techniques in practice could provide new possibilities in t...
|
PMID: 18631368
PDF is available here.
Abstract
Staphylococcus aureus is a leading cause of ventricular assist device-related infections. This study evaluated the protective effect against S. aureus infection of active and passive immunization that targeted 3 proteins involved in bacterial attachment to a murine intra-aortic polyurethane patch. A...
|
PMID: 18613794
PDF is available here.
Abstract
We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 microg) with oxacillin disks (1 microg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than...
|
PMID: 19030733
PDF is available here.
Abstract
The high prevalence of clone z emphasizes the great capacity of CoNS to colonize patients with central venous catheters such as haemodialysis patients and personnel. This emphasizes the need for the establishment of control and prevention measures....
|
PMID: 18305314
PDF is available here.
Abstract
Therapeutic conclusions for staphylococcal implant infections treated with debridement and implant retention can only be drawn from a small series. To this aim, data from patients with implant staphylococcal infections (1998-2006) treated with debridement and implant retention were retrospectively r...
|
PMID: 18571387
PDF is available here.