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Ferredoxins (2):
Recent article
Ferredoxins
Abstract
The unicellular green alga Chlamydomonas reinhardtii has at least six plant-type ferredoxins (FDX). Besides the long-known photosynthetic ferredoxin PetF the isoforms Fdx2-Fdx6 have been identified. The FDX genes are differentially expressed under various environmental conditions suc...
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PMID: 20696493
PDF is available here.
PDF is available here.
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Abstract
We show that inverted membrane vesicles of A. woodii couple electron transfer from reduced ferredoxin to NAD(+) with the transport of Na(+) from the outside into the lumen of the vesicles. Na(+) transport was electrogenic, and accumulation was inhibited by sodium ionophores but not protonophores, de...
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PMID: 20921383
PDF is available here.
PDF is available here.
Current Microbiology (61)1 2010
Abstract
The marine bacterium Neptuniibacter sp. strain CAR-SF utilizes carbazole as its sole carbon and nitrogen sources. Two sets of clustered genes related to carbazole degradation, the upper and lower pathways, were obtained. The marine bacterium genes responsible for the upper carbazole degradation path...
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PMID: 20039169
PDF is available here.
PDF is available here.
Abstract
We characterized the regioselectivity of lauric acid by CYP119 using the auxiliary redox partner proteins putidaredoxin (Pd) and putidaredoxin reductase (PdR). Purified CYP119 protein showed a tight binding affinity to lauric acid (K(d)=1.1+/-0.1 microM) and dominantly hydroxylated (omega-1) positio...
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PMID: 20372030
PDF is available here.
PDF is available here.
Abstract
We have identified a novel subtype of Rieske ferredoxins present in hyperthermophilic archaea, in which a two-cysteine conserved SKTPCX((2-3))C motif is found at the C-terminus. We establish that in the Acidianus ambivalens representative, Rieske ferredoxin 2 (RFd2), these cysteines form a novel dis...
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PMID: 19862563
PDF is available here.
PDF is available here.
Infection and Immunity (78)2 2010
Abstract
We previously showed that cytotoxic necrotizing factor 1 (CNF1) contributes to Escherichia coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and interacts with the receptor on the surface of HBMEC. CNF1 is the cytoplasmic protein, and it remains incompletely understood how CNF1...
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PMID: 19917710
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)3 2010
Abstract
We present a detailed analysis of a Methanosarcina mazei Delta ech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduc...
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PMID: 19948802
PDF is available here.
PDF is available here.
Abstract
The general importance of the Fe-S cluster prosthetic groups in biology is primarily attributable to specific features of iron and sulfur chemistry, and the assembly and interplay of the Fe-S cluster core with the surrounding protein is the key to in-depth understanding of the underlying mechanisms....
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PMID: 20885930
PDF is available here.
PDF is available here.
Biotechnology Letters (31)9 2009
Abstract
We report the functional expression in Escherichia coli of tricistronic constructs consisting of P450( cam ) encoded by the first cistron and the auxiliary proteins, Pd and PdR by the second and the third. Transformed bacterial whole cells efficiently oxidized (1R)-(+)-camphor to 5-exo-hydroxycampho...
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PMID: 19458919
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)31 2009
Abstract
The bfrB gene from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli. The resultant protein (BfrB), which assembles into a 445.3 kDa complex from 24 identical subunits, binds 12 molecules of heme axially coordinated by two Met residues. BfrB, isolated with 5-10 iron atoms per prote...
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PMID: 19575528
PDF is available here.
PDF is available here.
Abstract
Fatty acid desaturation in plastids and chloroplasts depends on the electron-donor activity of ferredoxins. Using degenerate oligonucleotides designed from known photosynthetic and heterotrophic plant ferredoxin sequences, two full-length ferredoxin cDNAs were cloned from sunflower (Helianthus annuu...
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PMID: 19130032
PDF is available here.
PDF is available here.
Abstract
We report on spectroscopic and redox characterization of the [Fe4S4] center in Synechocystis sp. PCC 6803 H86Y ferredoxin:thoredoxin reductase in the accessible redox states of both the as purified and N-ethylmaleimide-modified forms, using the combination of UV-visible absorption and variable-tempe...
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PMID: 19132843
PDF is available here.
PDF is available here.
Abstract
Transformation of dinitrosyl iron complexes (DNICs) [(NO)2Fe(SR)2]− (R = Et, Ph) into [4Fe-4S] clusters [Fe4S4(SPh)4]2− in the presence of [Fe(SPh)4]2−/1− and S-donor species S8 via the reassembling process ([(NO)2Fe(SR)2]− → [Fe4S3(NO)7]− (1)/[Fe4S3(NO)7]2− (2) → [Fe4S4(NO)4]2− (...
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PMID: 19053409
PDF is available here.
PDF is available here.
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory (112)50 2008
Abstract
We combine protein-protein docking algorithms, protein structure prediction methodologies and mixed quantum mechanics/molecular mechanics techniques to map the electron transfer pathway between cytochrome P450 camphor and its redox partner, putidaredoxin. Although the mechanism of interaction and el...
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PMID: 18823106
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1784)11 2008
Abstract
We use biophysical methods to investigate what is the relative contribution of iron-sulfur clusters in respect to ionic interactions in a thermophilic di-cluster ferredoxin model. Changes in protonation affect both the stability and the conformational dynamics of the protein fold. In the pH 5.5-8 in...
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PMID: 18534203
PDF is available here.
PDF is available here.
Biophysical Journal (95)8 2008
Abstract
We have simulated the process over multiple timescales. A Brownian dynamics simulation method gave an initial thorough sampling of the rigid-body translational and rotational phase spaces, and the resulting trajectories were used to compute the occupancy and free-energy landscapes. Several important...
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PMID: 18621810
PDF is available here.
PDF is available here.
Photosynthesis Research (97)3 2008
Abstract
I (PSI) were grouped and compared. PsaA, psaB, psaC, and petG represent genes that have been retained in the chloroplasts of both green- and red-lineage species. PsaD, psaE, psaF, and petF represent genes that have been retained in the chloroplast of red-lineage species, but have been transferred to...
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PMID: 18661249
PDF is available here.
PDF is available here.
Acta Crystallographica Section D (64)Pt 9 2008
Abstract
The 2.07 A resolution X-ray crystal structure of a soluble Rieske-type ferredoxin from Mus musculus encoded by the gene Mm.266515 is reported. Although they are present as covalent domains in eukaryotic membrane oxidase complexes, soluble Rieske-type ferredoxins have not previously been observed in...
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PMID: 18703841
PDF is available here.
PDF is available here.
Abstract
We have modeled here the interactions between FNR and both of its protein partners, Fd and Fld, using surface energy analysis, computational rigid-body docking simulations, and interface side-chain refinement. The results, consistent with previous experimental data, suggest the existence of alternat...
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PMID: 18260112
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 8 2008
Abstract
We show that the chloroplast precursor of ferredoxin-5 (Fd V) from maize (Zea mays) interacts with the multifunctional HC-Pro protein of sugar cane mosaic virus (SCMV) in yeast, Nicotiana benthamiana cells and maize protoplasts. Our results demonstrate that the transit peptide rather than the mature...
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PMID: 18632977
PDF is available here.
PDF is available here.
Abstract
Three genes within the genome of E. coli K12 are predicted to encode proteins containing the typical Rieske iron-sulfur cluster-binding motifs. Two of these, hcaC and yeaW, were overexpressed in E. coli BL21 and Tuner (DE3) pLacI. The recombinant proteins were purified and analyzed by UV/Vis- and EP...
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PMID: 18286376
PDF is available here.
PDF is available here.
Transgenic Research (17)4 2008
Abstract
The ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small...
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PMID: 17710559
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)15 2008
Abstract
We provide evidence that hydroquinone is further degraded through 4-hydroxymuconic semialdehyde and maleylacetate to beta-ketoadipate. The P. fluorescens ACB genes involved in 4-hydroxyacetophenone utilization were cloned and characterized. Sequence analysis of a 15-kb DNA fragment showed the presen...
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PMID: 18502868
PDF is available here.
PDF is available here.
Abstract
We focus on the structure and mechanism of action of members of the photosynthetic Fdx/Trx system and on biochemical processes linked to Trx. We also summarize recent evidence that extends the Fdx/Trx system to amyloplasts-heterotrophic plastids functional in the biosynthesis of starch and other cel...
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PMID: 18377232
PDF is available here.
PDF is available here.
Abstract
Endocrine Journal (55)3 2008
Abstract
We previously reported that CS increased in the endometrium during implantation, suggesting that CS plays an important role in reproduction. It has been reported that CS regulates progesterone and pregnenolone production in the placenta, adrenal glands and ovary. The regulatory mechanisms of steroid...
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PMID: 18490834
PDF is available here.
PDF is available here.
Physiologia Plantarum (133)3 2008
Abstract
We have used a Ds-T-DNA insertion line of Arabidopsis thaliana for the gene encoding the major leaf ferredoxin (Fd2, At1g60950) to create a situation of high electron pressure in the thylakoids. Although these plants (Fd2-KO) possess only the minor fraction of leaf Fd1 (At1g10960), they grow photoau...
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PMID: 18494733
PDF is available here.
PDF is available here.
Inorganic Chemistry (47)12 2008
Abstract
We have also shown that electronic localization generally favors small gav and large Jeff values (while the opposite is true for electronic delocalization) in a comparative study of the spectroscopic features of plant-type ferredoxins (Fd's) and Rieske centers (and related mutants). Two other types...
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PMID: 18491857
PDF is available here.
PDF is available here.
Abstract
We report on some recent findings, which significantly increased the understanding of the structure-function relationships of FNRs, i.e. the ability of adrenodoxin reductase and its homologs to catalyze the oxidation of NADP(+) to its 4-oxo derivative, and the properties of plant-type FNRs from non-...
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PMID: 18307973
PDF is available here.
PDF is available here.
Biotechnology Letters (30)6 2008
Abstract
I type electron-transfer system in E. coli BL21 (DE3). This was carried out using two plasmids with different selection markers and compatible origins of replication. The study results showed that this biotransformation system was able to mediate the O-dealkylation of 7-ethoxycumarin....
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PMID: 18259876
PDF is available here.
PDF is available here.
Biochemistry (77)1 2008
Abstract
Iron-sulfur (Fe/S) proteins are involved in a wide variety of cellular processes such as enzymatic reactions, respiration, cofactor biosynthesis, ribosome biogenesis, regulation of gene expression, and DNA-RNA metabolism. Assembly of Fe/S clusters, small inorganic cofactors, is assisted by complex p...
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PMID: 18366324
PDF is available here.
PDF is available here.
Abstract
We detected a conformational change in CYP101 upon binding of Pdx that reorients bound camphor appropriately for hydroxylation. Experimental evidence shows that binding of Pdx converts a single X-proline amide bond in CYP101 from trans or distorted trans to cis. Mutation of proline 89 to isoleucine...
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PMID: 18513977
PDF is available here.
PDF is available here.
Human Reproduction (23)5 2008
Abstract
Microarray technologies are useful to mine the transcriptome of FCs expressed in follicles associated with competent oocytes and could be used to improve embryo selection with the objective of successful single embryo transfer....
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PMID: 18310048
PDF is available here.
PDF is available here.
EMBO Journal (27)7 2008
Abstract
Glutaredoxins (Grxs) are small oxidoreductases that reduce disulphide bonds or protein-glutathione mixed disulphides. More than 30 distinct grx genes are expressed in higher plants, but little is currently known concerning their functional diversity. This study presents biochemical and spectroscopic...
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PMID: 18354500
PDF is available here.
PDF is available here.
Abstract
We tested whether deletion of the gene encoding the transcriptional negative regulator, IscR, could enhance the ability of Escherichia coli BL21 to synthesize active recombinant H2-pathway components and stimulate ferredoxin-dependent H2-accumulation in the presence or absence of oxygen. Under anoxi...
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PMID: 18320190
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)14 2008
Abstract
We report here the biochemical characterization of Chlamydomonas GRX1 and GRX3, the major cytosolic and chloroplastic isoforms, respectively. Glutaredoxins are classified on the basis of the amino acid sequence of the active site. GRX1 is a typical CPYC-type GRX, which is reduced by GSH and exhibits...
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PMID: 18216016
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)12 2008
Abstract
I (PSI) is a large membrane protein that catalyzes light-driven electron transfer across the thylakoid membrane from plastocyanin located in the lumen to ferredoxin in the stroma. Metal analysis reveals that PSI isolated from the cyanobacterial membranes of Synechococcus leopoliensishas a near-stoic...
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PMID: 18293937
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)12 2008
Abstract
We determined the crystal structure of Pyrococcus abyssi ABCE1 in complex with Mg(2+) and ADP to 2.8A resolution. ABCE1 consists of four structural domains. Two nucleotide binding domains are arranged in a head-to-tail orientation by a hinge domain, suggesting that these domains undergo the characte...
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PMID: 18160405
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)11 2008
Abstract
We studied the Tic component and the putative redox sensor Tic62 in more detail. The experimental results provide evidence that Tic62 can act as a bona fide dehydrogenase in vitro, and that it changes its localization in the chloroplast dependent on the NADP+/NADPH ratio in the stroma. Moreover, the...
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PMID: 18180301
PDF is available here.
PDF is available here.
FEBS Letters (582)5 2008
Abstract
I and II, a [3Fe-4S](1+/0) and a [4Fe-4S](2+/1+) centres. This is in contrast with the previously available ferredoxin structure from Sulfolofus tokodai, which was obtained from an artificial oxidative conversion with two [3Fe-4S](1+/0) centres and poor definition around cluster II....
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PMID: 18258200
PDF is available here.
PDF is available here.
Abstract
Combined QM/MM calculations of the active-site of cytochrome P450cam have been performed before and after the binding of P450cam to putidaredoxin. The calculations were carried out for both a 5-coordinated and a 6-coordinated active-site of cytochrome P450cam, with either a water molecule or a carbo...
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PMID: 18180042
PDF is available here.
PDF is available here.
Current Microbiology (56)3 2008
Abstract
We focused our biophysical and biochemical investigations on CAC0303 and CAC3527, which possess the sequence signature and length of classical 2[4Fe4S] clostridial ferredoxins but differ significantly in theoretical pI. After cloning, heterologous expression in E. coli followed by in vitro Fe-S inco...
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PMID: 18074176
PDF is available here.
PDF is available here.
Abstract
Acetogens use the Wood-Ljungdahl pathway for reduction of carbon dioxide to acetate. This pathway not only allows reoxidation of reducing equivalents during heterotrophic growth but also supports chemolithoautotrophic growth on H(2) + CO(2). The latter argues for this pathway being a source for net...
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PMID: 18378592
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)3 2008
Abstract
We report here that ferredoxin reduction with NADH in cell extracts from Clostridium kluyveri is catalyzed by the butyryl-CoA dehydrogenase/Etf complex and that the acetyl-CoA dependence previously observed is due to the fact that the cell extracts catalyze the reduction of acetyl-CoA with NADH via...
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PMID: 17993531
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)3 2008
Abstract
Abstract
We found that mitochondria isolated from wild-type yeast contain a pool of apoaconitase and machinery capable of forming new clusters and inserting them into this endogenous apoprotein pool. These observations allowed us to develop assays to assess the role of nucleotides (GTP and ATP) in cluster bi...
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PMID: 18029354
PDF is available here.
PDF is available here.
Abstract
We have tested a comprehensive representation of hexacoordinate hemoglobins from plants (rice hemoglobin), animals (neuroglobin and cytoglobin), and bacteria (Synechocystis hemoglobin) for their abilities to scavenge NO compared to myoglobin. Our experiments include in vitro comparisons of NO dioxyg...
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PMID: 18446211
PDF is available here.
PDF is available here.
Abstract
We describe the localization of two such enzymes in E. cuniculi cells. Immunofluorescent localization of ferredoxin involved in mitochondrial iron-sulfur cluster assembly reveals a punctate distribution as expected for mitochondria. In contrast, localization of mitochondrial glycerol-3-phosphate deh...
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PMID: 18318866
PDF is available here.
PDF is available here.
Abstract
The enzymes and pathways of steroidogenesis are familiar to most endocrinologists, but the biochemistry and molecular biology of these processes are still being studied. This chapter outlines current knowledge about each enzyme. The quantitative regulation of steroidogenesis occurs at the first step...
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PMID: 18493130
PDF is available here.
PDF is available here.
BMC Biochemistry (9)1 2008
Abstract
We present a scheme for classification of RHOs reflecting new sequence information and interactions between RHO enzyme components. RESULT: We have analyzed a total of 130 RHO enzymes in which 25 well-characterized RHO enzymes were used as standards to test our hypothesis for the proposed classificat...
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PMID: 18387195
PDF is available here.
PDF is available here.
Protein Science (5)9 2008
Abstract
The crystal structure of the 2[4Fe-4S] ferredoxin from Chromatium vinosum has been solved by molecular replacement using data recorded with synchrotron radiation. The crystals were hexagonal prisms that showed a strong tendency to develop into long tubes. The hexagonal prisms diffracted to 2.1 Å res...
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PMID: 8880900
PDF is available here.
PDF is available here.
Abstract
Trichomonas vaginalis, the causative agent for human trichomoniasis, is a problematic sexually transmitted disease mainly in women. At present, metronidazole-resistant trichomoniasis is an infrequent but challenging problem with no universally successful treatment. Genetic mutation is believed to be...
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PMID: 18445954
PDF is available here.
PDF is available here.
Abstract
A recently established mixed culture fermentation (MCF) model has been modified to account for the role of different electron carriers in the process. The MCF-model predicts the product spectrum as a function of the actual environmental conditions using a thermodynamic optimization criterion while s...
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PMID: 18359986
PDF is available here.
PDF is available here.
Biochemistry (New York) (73)1 2008
Abstract
I and II, and plastoquinone in oxygen reduction are examined. It is concluded that, even in the presence of ferredoxin and ferredoxin + NADP+, oxygen reduction is carried out mainly by the membrane-bound carriers of the photosynthetic electron transport chain. The hypothesis is put forward that most...
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PMID: 18294139
PDF is available here.
PDF is available here.
Abstract
A mixed quantum/classical investigation of the dynamical magnetostructural properties, that is, "magnetodynamics," of oxidized Anabaena PCC7119 ferredoxin is carried out at room temperature in two distinct conformational states. This protein hosts a [2Fe-2S] cluster in which two iron centers are ant...
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PMID: 18093955
PDF is available here.
PDF is available here.
FEBS Letters (581)29 2007
Abstract
We assume that desulfoferrodoxin is the key factor in the superoxide reductase dependent part of an alternative pathway for detoxification of reactive oxygen species in this obligate anaerobic bacterium....
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PMID: 18005665
PDF is available here.
PDF is available here.
Abstract
A persistent puzzle in the field of biological electron transfer is the conserved iron-sulfur cluster motif in both high potential iron-sulfur protein (HiPIP) and ferredoxin (Fd) active sites. Despite this structural similarity, HiPIPs react oxidatively at physiological potentials, whereas Fds are r...
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PMID: 18048692
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (277)1 2007
Abstract
Haloferax mediterranei is a halophilic archaeon that can grow using nitrate as the sole nitrogen source. A ferredoxin that serves as the physiological electron donor to the nitrate and nitrite reductases in this assimilatory process has been characterized. The ferredoxin was found to contain approxi...
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PMID: 17986084
PDF is available here.
PDF is available here.
Current Microbiology (55)6 2007
Abstract
We have studied the function of sanH and sanI. Disruption of sanH abolished the production of nikkomycin X and Z, but it accumulated one dominant component nikkomycin Lx, which is the nikkomycin X analog lacking the hydroxy group at the pyridyl residue. The sanI disruption mutant accumulated predomi...
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PMID: 17899263
PDF is available here.
PDF is available here.
Current Microbiology (55)6 2007
Abstract
The [2Fe-2S] cluster containing ferredoxin has attracted much attention in recent years. Genetic analyses show that it has an essential role in the maturation of various iron-sulfur (Fe-S) proteins and functions as a component of the complex machinery responsible for the biogenesis of Fe-S clusters....
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PMID: 17909890
PDF is available here.
PDF is available here.