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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Luciferases, Bacterial (0):
Recent article
Luciferases, Bacterial
Abstract
Bioluminescence imaging (BLI) is emerging as a powerful tool for real-time monitoring of infections in living animals. However, since luciferases are oxygenases, it has been suggested that the requirement for oxygen may limit the use of BLI in anaerobic environments, such as the lumen of the gut. St...
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PMID: 19880653
PDF is available here.
PDF is available here.
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Abstract
The in vitro coupled bacterial luciferase: FMN-NADH oxidoreductase bioluminescent system was: 1 mL crude extract, 27 mmol/L Dodecane 100 microL, 10 mmol/L FMN-Na 0.5 microL and 0.14 mmol/L NADH 300 microL. Furthermore, we developed a method for quantitative detection of NADH according to the biolumi...
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PMID: 20030062
PDF is available here.
PDF is available here.
Biophysical Chemistry (141)1 2009
Abstract
We hypothesize that the hydrophobicity of the quinone plays a role in the non-specific inhibition mechanism of xenobiotic molecules in the bacterial bioluminescence system via altering the rotational mobility of the endogenous flavin in the luciferase....
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PMID: 19162392
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (289)2 2008
Abstract
Two whole-cell bioluminescent reporters were constructed by fusing the reporter genes luxAB with the Co(2+) and Zn(2+) inducible coaT promoter or the Ni(2+)-inducible nrsBACD promoter, respectively, in the genome of Synechocystis sp. PCC 6803. The obtained reporters, designated coaLux and nrsLux, re...
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PMID: 19016871
PDF is available here.
PDF is available here.
Environmental Science (29)11 2008
Abstract
Recombinate luminescence bacteria have the important role in evaluating water toxicity. Two recombinate luminescence bacteria vectors PUCD-uvrA and PUCD-alkA were constructed to investigate the impaired mechanism of pollutant genetic toxicity. The genes of uvrA and alkA were amplified by PCR from E....
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PMID: 19186821
PDF is available here.
PDF is available here.
Abstract
These results show that the sensitivity of the two biosensors is sufficient for the detection of Hg2+ under most contaminated environments....
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PMID: 18437376
PDF is available here.
PDF is available here.
Abstract
We describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal an...
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PMID: 18204457
PDF is available here.
PDF is available here.
Abstract
We introduce the alternative approach of chemiluminescence (CL) detection with firefly luciferase as the probe. Firefly luciferase is roughly 100 times more efficient and is faster in generating CL than bacterial luciferase but requires the introduction of luciferin, a species that is not native to...
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PMID: 18179241
PDF is available here.
PDF is available here.
Abstract
We showed that some types of highly mineralized water with a high hydrocarbonate content have a marked inhibitory effect on the luminescence intensity of microbial luminescent biosensors, mimicking the effect of chemical pollutants....
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PMID: 18663957
PDF is available here.
PDF is available here.
Aquatic Toxicology (85)3 2007
Abstract
The bioassay Lux-Fluoro test was developed for the rapid detection and quantification of environmental pollutants with genotoxic and/or cytotoxic potential. This bacterial test system uses two different reporter genes whose gene products and their reactions, respectively, can be measured easily and...
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PMID: 17936920
PDF is available here.
PDF is available here.
Abstract
This study deals with application of bioluminescent assay systems to evaluate the detoxifying effect of humic substances (HS) on the solutions of organic oxidizers - quinones. A series of homologous quinones with different redox characteristics: 1,4-benzoquinone, tetrafluoro-1,4-benzoquinone, methyl...
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PMID: 17716903
PDF is available here.
PDF is available here.
Abstract
These results suggest that mutation of Glu175 to Gly extended the structure of Xenorhabdus Luminescence luciferase, locally....
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PMID: 17669259
PDF is available here.
PDF is available here.
Abstract
We report for the first time real-time imaging of lysis and leakage of single bacterium with 10-s temporal resolution. Movies are generated to visualize how the cell membrane was damaged by phage lysis, antibiotics attack, or dehydration, as well as the wall repair and cell recovery processes. The r...
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PMID: 17550231
PDF is available here.
PDF is available here.
Abstract
An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during...
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PMID: 17351089
PDF is available here.
PDF is available here.
Biosensors and Bioelectronics (22)9-10 2007
Abstract
We have developed a method for determining ultralow amounts of nisin in food samples that is based on luminescent biosensor bacteria. Modified bacterial luciferase operon luxABCDE was placed under control of the nisin-inducible nisA promoter in plasmid pNZ8048, and the construct was transformed into...
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PMID: 16996730
PDF is available here.
PDF is available here.
Abstract
Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated with virulence. Cloning and sequencing of the V. salmonicida lux operon revealed that homologs of all...
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PMID: 17277225
PDF is available here.
PDF is available here.
Abstract
Salmonellae are mammalian pathogens that are transmitted mainly through foodstuffs and their handlers. Rapid detection requires both specificity and sensitivity in samples containing other bacteria. A solution to this problem is the use of the great specificity conferred by bacteriophages. After imp...
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PMID: 18363229
PDF is available here.
PDF is available here.
Abstract
The unicellular cyanobacterium Synechococcus elongatus PCC 7942 is the model organism for studying prokaryotic circadian rhythms. Although S. elongatus does not display an easily measurable overt circadian behavior, its gene expression is under circadian control; hence, a "behavior" is created by li...
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PMID: 17417005
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)6 2007
Abstract
We therefore constructed a synthetic luxCDABE operon encoding the P. luminescens Lux proteins optimized for expression in high-GC bacteria. We tested the genes using transcriptional fusions to S. coelicolor promoters having well-established expression profiles during this organism's life cycle. The...
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PMID: 17337439
PDF is available here.
PDF is available here.
Abstract
Several lines of evidence have been reported previously to document the ability of the Vibrio harveyi NADPH-specific flavin reductase FRP to directly transfer reduced riboflavin-5'-phosphate to luciferase for bioluminescence. This study aimed at characterizing further the kinetic properties of FRP i...
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PMID: 16949542
PDF is available here.
PDF is available here.
Abstract
We successfully constructed a genetically labelled isoproturon-mineralizing-strain SRS2 and demonstrated its ability to survive in soil and its colonization of rhizosphere. SIGNIFICANCE AND IMPACT OF THE STUDY: The construction of a luxAB-labelled strain SRS2 maintaining the degradative ability, pro...
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PMID: 16910932
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (90)2 2006
Abstract
We were able to identify an orthologue of luxS, required for the synthesis of AI-2 signals, in Streptococcus anginosus. Comparative analyses revealed conserved sequences in the predicted S. anginosus LuxS. Expression of luxS was highest during early exponential growth phase. Compared to other oral s...
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PMID: 16897563
PDF is available here.
PDF is available here.
Abstract
Root proliferation as a response to exploit zones of nutrient enrichment in soil has been demonstrated for a wide range of plant species. However, the effectiveness of this as a strategy to acquire nutrients is also dependent on interactions with the soil microbial community. Specifically, C-flow fr...
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PMID: 16766600
PDF is available here.
PDF is available here.
Folia Microbiologica (51)1 2006
Abstract
We created Wluxl with the wild-type sequence and pWlux2, pWlux3 and pWlux4 plasmids containing TGA-, TAG- and TAA-stop codons, respectively. Streptomyces lividans TK 24 was transformed with the plasmids and the reporter system was tested by growth of the strain in the presence of streptomycin as a t...
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PMID: 16821714
PDF is available here.
PDF is available here.
Abstract
We found an inverse correlation between tubulin mRNA and the protein levels of free alpha- and beta-tubulin subunits, which are more abundant in trypomastigotes and amastigotes than in epimastigotes. Here we investigated a possible autoregulatory mechanism responsible for the differential accumulati...
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PMID: 16233898
PDF is available here.
PDF is available here.
Abstract
Our findings show that cag+H pylori cause rapid induction of Egr-1 in gastric epithelial cells which may contribute to H pylori mediated pathogenesis....
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PMID: 15863471
PDF is available here.
PDF is available here.
Current protocols in microbiology (Chapter 16) 2005
Abstract
This unit describes the analysis of the translation-regulating properties of viral RNAs in cell-based assays using a luciferase reporter system. Electroporation and polyethylene glycol-mediated introduction of luciferase reporter RNA into cowpea protoplasts are also presented. The Commentary section...
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PMID: 18770560
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)39 2005
Active site hydrophobicity is critical to the bioluminescence activity of Vibrio harveyi luciferase.
Abstract
Vibrio harveyi luciferase is an alphabeta heterodimer containing a single active site, proposed earlier to be at a cleft in the alpha subunit. In this work, six conserved phenylalanine residues at this proposed active site were subjected to site-directed mutations to investigate their possible funct...
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PMID: 16185065
PDF is available here.
PDF is available here.
FEBS Letters (579)21 2005
Abstract
Structural properties of a bacterial luciferase mutant, evolved by random mutagenesis, have been investigated. Bacterial luciferases (LuxAB) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. By random mutagenesis, one of t...
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PMID: 16098518
PDF is available here.
PDF is available here.
Journal of Bacteriology (187)9 2005
Abstract
Vibrio cholerae has multiple survival strategies which are reflected both in its broad distribution in many aquatic environments and its high genotypic diversity. To obtain additional information regarding the content of the V. cholerae genome, suppression subtractive hybridization (SSH) was used to...
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PMID: 15838025
PDF is available here.
PDF is available here.
Archives of Microbiology (183)3 2005
Abstract
We demonstrate that in the presence of various oxidants (hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide and ferrous ions) growth of dark mutants of different strains of Vibrio fischeri and Photobacterium leiognathi is impaired relative to wild-type bacteria, though to various extents...
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PMID: 15717160
PDF is available here.
PDF is available here.
Biochemical Journal (385)Pt 2 2005
Abstract
We have conducted random mutagenesis on this region. One of the mutants generated by a single mutation on LuxA at position 175 [E175G (Glu175-->Gly)] resulted in the 'slow decay' X. luminescens luciferase being converted into a luciferase with a significantly more rapid decay rate. These results ind...
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PMID: 15352872
PDF is available here.
PDF is available here.
Luminescence (20)3 2005
Abstract
The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluores...
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PMID: 15924327
PDF is available here.
PDF is available here.
Abstract
Biochemistry (Washington) (43)50 2004
Abstract
The excited state of 4a-hydroxy-4a,5-dihydroFMN has been postulated to be the emitter in the bacterial bioluminescence reaction. However, while the bioluminescence quantum yield of the luciferase emitter is about 0.16, chemiluminescence and fluorescence quantum yields of earlier flavin models mimick...
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PMID: 15595854
PDF is available here.
PDF is available here.
YAKUGAKU ZASSHI (124)10 2004
Abstract
These results indicate that the extinguishing by glucose in P. leiognathi is induced by the interruption of the translation of luciferase....
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PMID: 15467278
PDF is available here.
PDF is available here.
Abstract
We report a novel method for the rapid, sensitive, and quantitative detection of alkanes, alcohols, and aldehydes that relies on the reaction of bacterial luciferase with an aldehyde, resulting in the emission of light. Primary alcohols with corresponding aldehydes that are within the substrate rang...
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PMID: 15236245
PDF is available here.
PDF is available here.