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MutS DNA Mismatch-Binding Protein (0):
Recent article
MutS DNA Mismatch-Binding Protein
Abstract
We have developed a novel DNA-based nanomechanical device that measures the amount of work that a DNA-bending protein can do when it binds to the double helix. The device we report here is a scissors-like device consisting of two double-crossover (DX) molecules connected to each other by a flexible...
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PMID: 20205420
PDF is available here.
PDF is available here.
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Journal of Physical Chemistry B (113)36 2009
Abstract
Label-free and real-time detections of mismatched dsDNAs are demonstrated using MutS-protein-immobilized, single-walled carbon nanotube field effect transistor (SWNT-FET) devices. The E. coli MutS proteins specifically recognizing mismatched dsDNAs are immobilized on SWNT-FET devices that have been...
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PMID: 19685907
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)33 2009
Abstract
We term a "destabilized duplex", is involved in exonuclease-mediated strand degradation. We find that DNA strands in such a destabilized duplex can be displaced by the challenge of a molar excess of homologous single- and double-stranded DNA targets, in trans. Such an action of the UvrD protein is A...
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PMID: 19618961
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)8 2009
Abstract
We resolve this loop problem. We introduce AptaPIC, Aptamer-facilitated Protein Isolation from Cells, a technology that integrates (i) the development of aptamers for a protein in cell lysate and (ii) the utilization of the developed aptamers for protein isolation from the cell lysate. Using MutS pr...
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PMID: 19304751
PDF is available here.
PDF is available here.
Abstract
We first studied the role of mismatch repair proteins, MSH2, MSH3, MSH6, PMS2 and MLH1, since they would recognize mismatches. The MSH2-MSH6 heterodimer is involved in hypermutation by binding to U:G and other mismatches generated during repair synthesis, but the other proteins are not necessary. Se...
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PMID: 19008198
PDF is available here.
PDF is available here.
Abstract
This review focuses on the contribution of translesion DNA polymerases to immunoglobulin gene hypermutation, in particular on the roles of DNA polymerase eta (Poleta) in the generation of mutations at A/T bases from the initial cytosine-targeted activation-induced cytidine deaminase (AID)-mediated d...
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PMID: 19010770
PDF is available here.
PDF is available here.
Journal of Molecular Biology (384)1 2008
Abstract
We showed that MutS bound to a 30-bp heteroduplex containing an unpaired T with a binding affinity approximately 400-fold stronger than to a 30-bp homoduplex, a much higher specificity than previously reported. The binding of nucleotide cofactors decreased both MutS specific and nonspecific binding...
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PMID: 18773911
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)24 2008
Abstract
We used the same collection of isolates to explore the role of hypermutation in this process, since one of the hallmarks of CRI is the high prevalence of DNA mismatch repair (MMR) system-deficient mutator strains. The presence of mutations in 34 genes (many of them positively linked to adaptation in...
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PMID: 18849421
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)48 2008
Abstract
We found that Thermus thermophilus MutS2 (ttMutS2) harbors an endonuclease activity and that this activity is confined to the C-terminal domain, whose amino acid sequence is widely conserved in a variety of proteins with unknown function from almost all organisms ranging from bacteria to man. In thi...
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PMID: 18838375
PDF is available here.
PDF is available here.
Mutation Research (645)1-2 2008
Abstract
We have examined the consequences of seven MSH2 missense mutations found in HNPCC families by testing the MSH2 mutant proteins in functional assays as well as by generating equivalent missense mutations in Escherichia coli MutS and analyzing the phenotypes of these mutants. Here we show that two mut...
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PMID: 18822302
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)19 2008
Abstract
We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response to growth. In S. pyogenes, mutS and mutL are organized on a polycistronic mRNA under control of a common promoter. Prophage SF370.4...
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PMID: 18676670
PDF is available here.
PDF is available here.
Abstract
We examine the cellular response to fluorodeoxyuridine (FdU) and the role of the MMR system. After brief exposure of cells to low doses of FdU, MMR mediates DNA damage signaling during S-phase and triggers arrest in G2/M in the first cell cycle in a manner requiring MutSalpha, MutLalpha, and DNA rep...
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PMID: 18543256
PDF is available here.
PDF is available here.
Molecular Microbiology (69)6 2008
Abstract
SUMMARY: MutS protein dimer recognizes and co-ordinates repair of DNA mismatches. Mismatch recognition by the N-terminal mismatch recognition domain and subsequent downstream signalling by MutS appear coupled to the C-terminal ATP catalytic site, Walker box, through nucleotide-mediated conformationa...
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PMID: 18673453
PDF is available here.
PDF is available here.
Abstract
We examined the binding properties of this protein with various DNA substrates containing a single centrally located adduct of ruthenium(II) arene complexes [(eta(6)-arene)Ru(II)(en)Cl][PF(6)] [arene is tetrahydroanthracene (THA) or p-cymene (CYM); en is ethylenediamine]. These two complexes were ch...
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PMID: 18491151
PDF is available here.
PDF is available here.
Current Microbiology (57)1 2008
Abstract
These results suggest that inactivation of the PP pathway decreases the resistance of D. radiodurans cells to DNA damaging agents and increases mutation frequency and integration efficiency in the mutS1 mutant background....
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PMID: 18389309
PDF is available here.
PDF is available here.
Cell Research (18)5 2008
Abstract
We show that a mutant Arabidopsis plant carrying the putative disrupted AtMSH5 gene exhibits defects during meiotic division, producing a proportion of nonviable pollen grains and abnormal embryo sacs, and thereby leading to a decrease in fertility. AtMSH5 expression is confined to meiotic floral bu...
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PMID: 18379590
PDF is available here.
PDF is available here.
Mutation Research (640)1-2 2008
Abstract
We looked at the relationship between recognition and the effects of methylating versus ethylating agents on mutagenesis, using a MutS-deficient strain of E. coli. We find that while methylating agents induce mutations more effectively in a MutS-deficient strain than in wild-type, this genetic backg...
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PMID: 18243250
PDF is available here.
PDF is available here.
BioTechniques (44)2 2008
Abstract
We and others have previously identified factors that influence SSO-mediated single base substitutions. In this report, we focus our study on SSO-mediated deletion on plasmids. We found that SSOs as short as 63 bp were sufficient to mediate deletion as long as 2 kb with efficiency higher than 1%. St...
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PMID: 18330349
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Using digital differential display PCR, mouse ESTs exhibiting an oocyte-predominant expression were identified. Those murine ESTs were subsequently used to identify cognate rainbow trout (Oncorhynchus mykiss) ESTs using a reciprocal Blast search strategy. In the present study we report the identific...
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PMID: 18947432
PDF is available here.
PDF is available here.
Mutation Research (637)1-2 2008
Abstract
We show that E. colidam cells transformed with plasmids containing either the mutS, mutL or mutH gene display a mutation frequency three to eight times lower than that of the parental dam strain, due to increased mismatch-stimulated cell killing. Transformed strains are also more susceptible to kill...
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PMID: 17727900
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)2 2008
Abstract
These results suggest that the genus Oenococcus is hypermutable due to the loss of mutS and mutL, which occurred with the divergence away from the neighboring Leuconostoc branch. The hypermutable status of the genus Oenococcus explains the observed high level of allelic polymorphism among known O. o...
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PMID: 17993526
PDF is available here.
PDF is available here.
Abstract
The ability of MutS to recognize mismatched DNA is required to initiate a mismatch repair (MMR) system. ATP binding and hydrolysis are essential in this process, but their role in MMR is still not fully understood. In this study, steady-state ATPase activities of MutS from Escherichia coli were inve...
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PMID: 17950245
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (24)6 2007
Abstract
Six germline mutations of MSH6 gene were detected in 39 probands of Chinese HNPCC kindreds, and the mutations distributed in the exon 4, 6, 9 and 10. Four out of six mutations were missense mutation, one was nonsense mutation and the remaining one was insertion mutation in splice site. The results o...
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PMID: 18067074
PDF is available here.
PDF is available here.
Journal of Molecular Biology (374)1 2007
Abstract
We incorporated 2-aminopurine, a fluorescent analogue of adenine next to a G.T mismatch, a C.C mismatch, or an unpaired T, and at several other non-mismatch positions. Rotational diffusion of 2-aminopurine at these locations, monitored by time-resolved fluorescence anisotropy, showed distinct differ...
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PMID: 17919654
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (275)2 2007
Abstract
A variable but substantial proportion of wild Escherichia coli isolates present consistently lower mutation frequencies than that found in the ensemble of strains. The genetic mechanisms responsible for the hypo-mutation phenotype are much less known than those involved in hyper-mutation. Changes in...
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PMID: 17825069
PDF is available here.
PDF is available here.
Abstract
We determined that deletion of the last 7 C-terminal amino acids is enough to abolish tetramer formation and that the K850A substitution destabilize the tetramer structure. It is proposed that the C-terminal extreme alpha helix (residues 839-850) of the protein may play an important role in protein...
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PMID: 17599803
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (282)22 2007
Abstract
We demonstrate that the MutS tetramer is extended in solution using small angle x-ray scattering and the crystal structure of the C-terminal 34 amino acids of MutS containing the tetramer-forming domain fused to maltose-binding protein (MBP). Wild-type C-terminal MBP fusions formed tetramers and cou...
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PMID: 17426027
PDF is available here.
PDF is available here.
Journal of Molecular Biology (369)1 2007
Abstract
We propose that DNA bending could play a role in MutS biochemical modulations induced by a compound lesion and that cisplatin DNA damage signaling by the MMR system could be modulated in a direct mode....
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PMID: 17400248
PDF is available here.
PDF is available here.
Molecular Cell (26)4 2007
Abstract
We describe a series of crystal structures of human MutSalpha bound to different DNA substrates, each known to elicit one of the diverse biological responses of the MMR pathway. All lesions are recognized in a similar manner, indicating that diversity of MutSalpha-dependent responses to DNA lesions...
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PMID: 17531815
PDF is available here.
PDF is available here.
Abstract
We isolated a mutL mutant having a separation-of-function phenotype. The mutant showed an increased frequency of deletions but not of mutations. The split phenotype is due to a decreased MutL level, indicating that recombination, but not replication editing, is highly sensitive to MutL level. By alt...
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PMID: 17502621
PDF is available here.
PDF is available here.
Microbiology (153)Pt 5 2007
Abstract
These results suggest that P. aeruginosa hypermutation is associated with a biological cost, reducing the potential for colonization of new environments and therefore strain transmissibility....
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PMID: 17464058
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)9 2007
Abstract
These results may help in understanding the reasons for the low and high prevalences, respectively, of hypermutable P. aeruginosa strains in acute and chronic infections....
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PMID: 17307847
PDF is available here.
PDF is available here.
Plant Cell Online (19)4 2007
Abstract
We have observed reproducible asymmetric recombination events occurring at specific locations in the mitochondrial genome. Based on these experiments and existing models of double-strand break repair, we propose a model for plant mitochondrial DNA replication, chimeric gene formation, and the illegi...
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PMID: 17468263
PDF is available here.
PDF is available here.
Molecular Microbiology (64)2 2007
Abstract
Pseudomonas aeruginosa colonizes the respiratory tract of cystic fibrosis (CF) patients, where mutators along with mucoid variants emerge leading to chronic infection. Mucoid conversion generally involves mutations inactivating the mucA gene. This study correlates the frequency and nature of mucA mu...
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PMID: 17493134
PDF is available here.
PDF is available here.
Journal of Molecular Biology (366)4 2007
Abstract
We present a kinetic analysis of these interactions, including the effects of ATP binding and hydrolysis, reported directly from the mismatch site by 2-aminopurine fluorescence. When free of nucleotides, the Thermus aquaticus MutS dimer binds a mismatch rapidly (k(ON)=3 x 10(6) M(-1) s(-1)) and form...
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PMID: 17207499
PDF is available here.
PDF is available here.
Abstract
These results provide a basis for further studies aimed at resolving the genotype/phenotype relationships of meningococcal genome variability and mutator activity....
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PMID: 17284282
PDF is available here.
PDF is available here.
Abstract
A fluorescent method was developed for the detection of unpaired and mismatched DNAs using a MutS-fluorophore conjugate. The fluorophore, 2-(4'-(iodoacetoamido)anilino) naphthalene-6-sulfonic acid (IAANS), was site-specifically attached to the 469 position of Thermus aquaticus (Taq.) MutS mutant (C4...
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PMID: 16876990
PDF is available here.
PDF is available here.
Journal of Bacteriology (189)3 2007
Abstract
We have previously reported that the human MutY homolog (hMYH) physically and functionally interacts with the human MutS homolog, hMutSalpha (Y. Gu et al., J. Biol. Chem. 277:11135-11142, 2002). Here, we show that a similar relationship between MutY and MutS exists in E. coli. The interaction of Mut...
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PMID: 17114250
PDF is available here.
PDF is available here.
Journal of Biotechnology (127)2 2007
Abstract
A bifunctional protein consisting of MutS, a mismatch binding protein and a beta-galactosidase reporter domain has been constructed. The fusion of beta-galactosidase to the MutS C-terminus was obtained by cloning the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carryi...
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PMID: 16934899
PDF is available here.
PDF is available here.
Abstract
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demanded. A protein chip method for detection of single-base mismatches and unpaired bases of DNA...
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PMID: 18220231
PDF is available here.
PDF is available here.
Microbiology (153)Pt 1 2007
Abstract
In Pseudomonas aeruginosa, quorum sensing constitutes a highly complex cell-to-cell communication system that, along with the cognate acylhomoserine lactone signals and regulators LasR and RhlR, modulates the production of virulence factors and a wide range of metabolic functions. In a previous pape...
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PMID: 17185551
PDF is available here.
PDF is available here.
Nucleic Acids Research (35)22 2007
Abstract
We conducted systematic phylogenetic analysis of its key components, the bacterial MutS and MutL genes and their eukaryotic homologs. Based on genome-wide homolog searches, we identified three new MutS subfamilies (MutS3-5) in addition to the previously studied MutS1 and MutS2 subfamilies. Detailed...
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PMID: 17965091
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)41 2006
Abstract
Abstract
Journal of Biological Chemistry (281)32 2006
Abstract
We have constructed nicked circular heteroduplexes that contain a single MeG-T mispair, and we have examined processing of these molecules by mismatch repair in nuclear extracts of human cells. Excision provoked by MeG-T is restricted to the incised heteroduplex strand, leading to removal of the MeG...
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PMID: 16772289
PDF is available here.
PDF is available here.
Abstract
We present evidence for a convergent gene fusion involving a MutS-homologous gene functioning within the mitochondrion and designated Msh1. In only plants and soft corals, the MutS homologue has fused with a homing endonuclease sequence at the carboxy terminus of the protein. However, the endonuclea...
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PMID: 16830098
PDF is available here.
PDF is available here.
Abstract
TEM-1 and TEM(pUC19)beta-lactamases can gain activity against ceftazidime and other expanded-spectrum cephalosporins via point mutation. The frequency of emergent resistance to ceftazidime at 4 x MIC was elevated >or= 250-fold in hyper-mutable, MutS-deficient Escherichia coli harbouring these beta-l...
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PMID: 16842578
PDF is available here.
PDF is available here.
Abstract
We investigated the variability of MutS among Pseudomonas aeruginosa recovered from cystic fibrosis (CF) patients. Sequencing of the mutS gene of 15 hypermutable P. aeruginosa isolates obtained from different patients revealed high rates of nucleotide substitutions as compared to that of strain PAO1...
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PMID: 16542874
PDF is available here.
PDF is available here.
Abstract
We show that human MutLalpha is a latent endonuclease that is activated in a mismatch-, MutSalpha-, RFC-, PCNA-, and ATP-dependent manner. Incision of a nicked mismatch-containing DNA heteroduplex by this four-protein system is strongly biased to the nicked strand. A mismatch-containing DNA segment...
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PMID: 16873062
PDF is available here.
PDF is available here.
Abstract
The mismatch repair process corrects errors in newly synthesized DNA. In this issue, Modrich and colleagues (Kadyrov et al., 2006) show that a component of the human mismatch repair machinery, MutLalpha, has endonuclease activity. MutLalpha introduces single-strand breaks near the mismatch and thus...
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PMID: 16873053
PDF is available here.
PDF is available here.
Cancer Cell (9)6 2006
Abstract
The DNA mismatch repair (MMR) system maintains genome integrity by correcting replication errors. MMR also stimulates checkpoint and cell death responses to DNA damage suggested by the resistance of MMR-defective tumor cells to several chemotherapeutic agents. MMR-dependent cytotoxic response may re...
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PMID: 16766259
PDF is available here.
PDF is available here.
Molecular Cell (22)4 2006
Abstract
We show that checkpoint signaling in response to DNA methylation occurs during S phase and requires DNA replication that gives rise to O(6)-meG/T mispairs. DNA binding studies reveal that MutSalpha specifically recognizes O(6)-meG/T mispairs, but not O(6)-meG/C. In an in vitro assay, ATR-ATRIP, but...
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PMID: 16713580
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (281)20 2006
Abstract
We find that MutL interacts directly with the beta sliding clamp, a ring-shaped dimeric protein that confers processivity to DNA polymerases by tethering them to their substrates. Interestingly, the interaction of MutL with beta only occurs in the presence of single-stranded DNA. We find that the in...
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PMID: 16546997
PDF is available here.
PDF is available here.
Analytical Sciences (22)5 2006
Abstract
A novel electrochemical biosensor was developed to detect gene mutation by using a DNA-mismatch binding protein: MutS from Escherichia coli. The MutS protein was immobilized onto an Au-electrode surface via complex formation between a histidine tag of the MutS protein and a thiol-modified nitrilotri...
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PMID: 16770041
PDF is available here.
PDF is available here.
Microbiology (152)Pt 4 2006
Abstract
I strains were analysed using nucleotide sequences of four genes (mglA, proV, torC and speC). Fifteen strains from the Salmonella reference collection B (SARB), belonging to 13 serovars, were analysed. Sequence data of two housekeeping genes, mdh and mutS, of the same 15 strains reported by Brown et...
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PMID: 16549673
PDF is available here.
PDF is available here.
Abstract
We report simple identification of base pairs in SNPs without DNA sequencing. Our approach is based on the unique ability of MutS protein to bind different single-nucleotide mismatches in DNA with different affinities. Conceptually, the DNA in question is mixed with reference DNA, melted, and reanne...
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PMID: 16536443
PDF is available here.
PDF is available here.
Abstract
We have optimized conditions for investigation of MutLalpha.MutSalpha complexes using a DNA pulldown assay. Non-specific DNA end-binding, which frequently interfered with analysis of the interaction, did not occur under the applied conditions. MutSalpha had significantly higher affinity to DNA mispa...
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PMID: 16403449
PDF is available here.
PDF is available here.
Journal of Biotechnology (121)2 2006
Abstract
We constructed chimeric proteins consisting of MutS from Thermus thermophilus and GFPuv from Aequorea victoria by cloning the GFPuv gene into the plasmid vectors carrying the mutS gene. The GFPuv domain fused to the N-terminus of MutS (histag-GFP-MutS) exhibited the same level of green fluorescence...
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PMID: 16157410
PDF is available here.
PDF is available here.
EMBO Journal (25)2 2006
Abstract
We have analyzed the crystal structures, DNA binding and the response to ATP binding of three Glu38 mutants. While confirming the role of the negative charge in initial discrimination, we show that in vivo mismatch repair can proceed even when discrimination is low. We demonstrate that the formation...
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PMID: 16407973
PDF is available here.
PDF is available here.