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Staphylococcal Protein A (0):
Recent article
Staphylococcal Protein A
Xenobiotica (41)5 2011
Abstract
Immunoprecipitation (IP) uses the specificity of antibodies to isolate target proteins (antigens) out of complex sample mixtures. Three different approaches for performing IP will be discussed; traditional (classical) method, oriented affinity method and direct affinity method. The traditional metho...
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PMID: 18369874
PDF is available here.
PDF is available here.
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Abstract
Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affin...
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PMID: 20978976
PDF is available here.
PDF is available here.
Orthopaedic Nursing (23)8 2010
Abstract
Skin injuries provide a favorable environment for microbial infection if left untreated. This is problematic especially in nosocomial situations having a high prevalence of Staphylococcus aureus that can cause suppuration of wounds, systemic disease, and toxic shock. The objective of this investigat...
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PMID: 20664329
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
|
PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
|
PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
|
PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
|
PMID: 20564482
PDF is available here.
PDF is available here.
Abstract
Biomolecules, including protein A, albumin, and immunoglobulin G, are spotted on top of a nanoporous substrate by using a continuous-flow microspotter (CFM) system, which normally produces spots 3 to 4 orders of magnitude more sensitive than conventional biomolecule printing methods....
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PMID: 20564482
PDF is available here.
PDF is available here.
Analytica Chimica Acta (667)1-2 2010
Abstract
The Z-domain of protein A has been known to bind specifically to the F(c) region of antibodies (IgGs). In this work, the Z-domain of protein A was expressed on the outer membrane of Escherichia coli by using "Autodisplay" technology as a fusion protein of autotransport domain. The E. coli with autod...
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PMID: 20441874
PDF is available here.
PDF is available here.
Abstract
We detected "tumor necrosis factor-alpha (TNF-alpha)" on a gold nanoarray protein chip at the attomolar (aM) concentration level (x10(-18)) by total internal reflection fluorescence microscopy (TIRFM). The 4 x 5 nanoarray patterning of gold with a spot diameter of 500 nm was successfully achieved on...
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PMID: 20358928
PDF is available here.
PDF is available here.
Ginekologia polska (81)5 2010
Abstract
Biochemical markers and abnormal uterine artery Doppler waveform are associated with PIH and IUGR. These parameters can be the base for the test identifying pregnant patients with high risk of PIH and IUGR....
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PMID: 20568515
PDF is available here.
PDF is available here.
Abstract
ST22-methicillin-resistant Staphylococcus aureus type IV (ST22-MRSA-IV) is endemic in Irish hospitals and is designated antibiogram-resistogram type-pulsed-field group (AR-PFG) 06-01. Isolates of this highly clonal strain exhibit limited numbers of pulsed-field gel electrophoresis (PFGE) patterns an...
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PMID: 20335411
PDF is available here.
PDF is available here.
Abstract
We describe a simple, aqueous route for the one-pot synthesis of antibody-derivatized zinc sulfide (ZnS) immuno-QDs. In this strategy, easily expressed and purified fusion proteins perform the dual function of nanocrystal mineralizers through ZnS binding sequences identified by cell surface display...
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PMID: 20218715
PDF is available here.
PDF is available here.
Abstract
This study aimed to evaluate the use of protein A-peroxidase (horseradish peroxidase [HRPO]) in indirect enzyme-linked immunosorbent assays (iELISAs) and IgG avidity assays for serological distinction between Brucella abortus S19-vaccinated and -infected cows. Four groups were analyz...
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PMID: 20147498
PDF is available here.
PDF is available here.
Abstract
Abstract
spa typing is a method used in molecular typing of methicillin-resistant Staphylococcus aureus (MRSA). Due to the lack of an amplification product in one of 55 MRSA cases, a new PCR setup, which allows an indicative description of a previously unknown type, was performed.
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PMID: 19955280
PDF is available here.
PDF is available here.
Abstract
We used clumping factor A (clfA) to profile 87 bovine mastitis isolates from four regions in Canada and compared the results to those obtained by pulsed-field gel electrophoresis (PFGE) and spa typing. Twenty-five pulsotypes were obtained by PFGE with an index of discrimination of 0.91. These were a...
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PMID: 19955267
PDF is available here.
PDF is available here.
Abstract
The 2009 Viral Clearance Symposium (Indianapolis, IN, USA) was held to interactively discuss methods for virus removal and inactivation during biopharmaceutical manufacture. Its origin was the result of worldwide regulatory and industry recognition that challenges, gaps, and opportun...
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PMID: 21516942
PDF is available here.
PDF is available here.
Abstract
We collected 913 food samples and detected 93 S. aureus isolates by using culture and biochemical tests. Subsequently, the X region of the protein A gene was amplified by the polymerase chain reaction (PCR) and amplicons were digested with HaeII and HindIII. Seven different patterns, ranging in leng...
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PMID: 20130689
PDF is available here.
PDF is available here.
Abstract
TAP (tandem affinity purification) allows rapid and clean isolation of a tagged protein along with its interacting partners from cell lysates. Initially developed in yeast, the TAP method has subsequently been adapted to other cells and organisms. In combination with MS analysis, this method has bec...
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PMID: 20156193
PDF is available here.
PDF is available here.
médecine/sciences (25)12 2009
Abstract
Recent advances in combinatorial protein engineering have made it possible to develop non-Ig protein scaffolds that can potentially substitute for most whole antibody-associated properties. These protein scaffolds display most of the binding properties associated with the variable domain of antibodi...
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PMID: 20035700
PDF is available here.
PDF is available here.
Abstract
Staphylococcus aureus is one of the most important etiologic agents of ovine mastitis. To develop effective control measures for mastitis, it is important to type S. aureus strains that have considerable genetic heterogeneity. In the current study, 47 S. aureus strains isolated from ovine mastitis w...
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PMID: 19901288
PDF is available here.
PDF is available here.
Abstract
The use of PCR for molecular diagnosis is accepted as the standard method for detecting nucleic acids from numerous major infectious agents using diverse sampling techniques. Although PCR is an essential tool in the research laboratory; the success of the method depends on a sample free of inhibitor...
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PMID: 19539653
PDF is available here.
PDF is available here.
Abstract
We develop a highly sensitive quartz crystal microbalance (QCM) biosensor with a fundamental resonance frequency of 170 MHz. A naked AT-cut quartz plate of 9.7 microm thick is set in a sensor cell. Its shear vibration is excited by the line wire, and the vibration signals are detected by the other l...
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PMID: 19728731
PDF is available here.
PDF is available here.
Abstract
Protein A chromatography has become widely established for the preparative purification of mAbs (monoclonal antibodies). Low pH elution from Protein A columns followed by neutralization can often lead to precipitation of impurities in the product stream, leading to a visually turbid solution. Pretre...
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PMID: 19656082
PDF is available here.
PDF is available here.
Abstract
Affinity capture mass spectrometry was used to isolate and ionize protein A from Staphylococcus aureus from both a commercial source and cell culture lysate using matrix assisted laser desorption/ionization (MALDI) mass spectrometry. Two surfaces are compared: gold surfaces with immunoglobulin G cov...
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PMID: 19572565
PDF is available here.
PDF is available here.
Abstract
We show that, if contact energies are properly taken into account, a different picture emerges from kinetic simulations of the above-mentioned model. This is characterized by a dominant folding pathway, which is consistent with the most recent experimental results. Given the simplicity of the model,...
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PMID: 19624233
PDF is available here.
PDF is available here.
Abstract
These results provide the first report of a surface display system in lactic acid bacteria for the delivery of oral vaccines against Helicobacter pylori....
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PMID: 19267231
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that Staphylococcus aureus activates type I IFN signaling, which contributes to its virulence as a respiratory pathogen. This response was dependent on the expression of protein A and, more specifically, the Xr domain, a short sequence-repeat region encoded by DNA that consists...
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PMID: 19603548
PDF is available here.
PDF is available here.
Methods in Molecular Biology (551)551 2009
Abstract
The spa typing method is based on sequencing of the polymorphic X region of the protein A gene (spa), present in all strains of Staphylococcus aureus. The X region is constituted of a variable number of 24-bp repeats flanked by well-conserved regions. This single-locus sequence-based typing method c...
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PMID: 19521876
PDF is available here.
PDF is available here.
Abstract
Abstract
A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion...
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PMID: 19494700
PDF is available here.
PDF is available here.
Biomedical Microdevices (11)2 2009
Abstract
Although 3-aminopropyltriethoxysilane (APTES) is widely adopted as a monolayer in biosensors, experimental silanization takes at least 1 h at high temperature. Therefore, the feasibility of the silanization with APTES in a short reaction time and at room temperature was investigated. The surface mod...
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PMID: 18821014
PDF is available here.
PDF is available here.
Abstract
We propose a novel, alternative approach that can be applied in at least one instance: evaluating retroviral clearance by protein A chromatography. Our strategy uses a quantitative PCR (Q-PCR) assay that quantifies the endogenous type C retrovirus-like particle genomes directly in production Chinese...
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PMID: 18988264
PDF is available here.
PDF is available here.
Biomaterials (30)9 2009
Abstract
Nanoparticles are effective of delivering cargo into cells. Here, sodium borocaptate (BSH) was encapsulated in liposomes composed of nickel lipid, and anti-epidermal growth factor receptor (EGFR) antibodies were conjugated to the liposomes using the antibody affinity motif of protein A (ZZ) as an ad...
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PMID: 19121537
PDF is available here.
PDF is available here.
Abstract
We introduce a new way to perform swaps between replicas in replica exchange molecular dynamics simulations. The method is based on a generalized canonical probability distribution function and flattens the potential of mean force along the temperature coordinate, such that a random walk in temperat...
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PMID: 19239315
PDF is available here.
PDF is available here.
Pediatric Research (65)2 2009
Abstract
We describe 13 patients out of nine families with the clinical presentation of ALPS. Eight different mutations were detected. Sensitivity to CD95-induced cell-death, assembly of the CD95-death-inducing signaling complex (DISC), and activity of initiator caspases-8 and -10 were compared in EBV-transf...
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PMID: 18948840
PDF is available here.
PDF is available here.
Analytical Biochemistry (385)1 2009
Abstract
We previously reported a silica-binding protein, designated Si-tag, which can be used to immobilize proteins on silica surfaces. Here, we constructed a fusion protein of Si-tag and immunoglobulin-binding staphylococcal protein A for oriented immobilization of antibodies on a silicon wafer whose surf...
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PMID: 19017523
PDF is available here.
PDF is available here.
Abstract
Abstract
We believe that this mathematical model can be used to evaluate the significance of other putative interactions in the regulatory network governing spa transcription....
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PMID: 18657473
PDF is available here.
PDF is available here.
Abstract
Combining the procedures of immunoprecipitation and immunoblotting can help to overcome some of the limitations of each separate procedure. Immunoblotting can identify immunoprecipitated proteins more specifically and with higher sensitivity than nonspecific protein stains or autoradiography. Immuno...
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PMID: 19378065
PDF is available here.
PDF is available here.
Abstract
We describe the use of iodinated secondary antibodies for immunodetection of an autoantigen during HPLC purification....
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PMID: 19378082
PDF is available here.
PDF is available here.
Abstract
The combination of histochemical techniques and electron microscopy is a powerful tool to study the mechanisms and pathology of renal disease. Through the use of electron-dense markers such as colloidal gold, biologists are able to localize immune deposits, cellular receptors, and extracellular prot...
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PMID: 19148612
PDF is available here.
PDF is available here.
Abstract
All the strains resistant to oxacillin were found to contain mec A gene. Fifty-two per cent of these strains were typeable by the international basic set of 23 phages. Five different PCR-RFLP patterns were observed among 125 MRSA strains. Non phage typeable strains were differentiated into four PCR-...
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PMID: 19384033
PDF is available here.
PDF is available here.
Biophysical Journal (95)12 2008
Abstract
We have created a model polyQ protein in which the repeat location mimics the flexible environment of the polyQ tract in the disease proteins. Our model protein recapitulates the aggregation features observed with the clinical proteins and allows structural characterization. With the use of NMR spec...
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PMID: 18849414
PDF is available here.
PDF is available here.
Abstract
Optimal conditions for depositing protein microarrays using a continuous-flow microfluidic device, the continuous-flow microspotter (CFM), have been determined using a design of experiments approach. The amount of protein deposited on the surface depends on the rates of convective and diffusive tran...
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PMID: 18939855
PDF is available here.
PDF is available here.
Abstract
A unique, sensitive, highly specific, and photobleaching-resistant immunoassay system utilizing gold nanoparticles and surface-enhanced Raman scattering (SERS) is described. This new system, featuring a capability of bifunctional analysis, is manufactured by chemisorption of antibody immunoglobulin...
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PMID: 18468881
PDF is available here.
PDF is available here.
Amino Acids (35)3 2008
Abstract
We introduce a predicted contact map constructed based on the interresidue average-distance statistics for prediction of folding properties of a protein. We refer to this map as an average distance map (ADM). Furthermore, the statistics of interresidue distances can be converted to an effective inte...
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PMID: 18431532
PDF is available here.
PDF is available here.
Abstract
We conducted a prospective multicenter study of methicillin-resistant Staphylococcus aureus (MRSA) isolates, including the first five consecutive clinical isolates, collected between September 2006 and February 2007 in 23 hospitals located throughout France (Fig. 1). The 111 isolates were tested for...
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PMID: 18667599
PDF is available here.
PDF is available here.
Abstract
Gammaretroviral and lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein G (VSV-G) have been used for stable gene transfer because of their broad host range and high mechanical strength. In the present study, an expression plasmid for chimeric VSV-G, consisting of a ZZ fragmen...
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PMID: 18606189
PDF is available here.
PDF is available here.
Journal of Chromatography A (1205)1-2 2008
Abstract
The robustness of virus clearance with respect to protein A media reuse was demonstrated using media with four matrix chemistries: Protein A immobilized ProSep A, Poros A50, Protein A ceramic Hyper DF and MabSelect SuRe, an alkali resistant protein A ligand. Endogenous retrovirus clearance, step yie...
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PMID: 18722622
PDF is available here.
PDF is available here.
Journal of Molecular Biology (381)5 2008
Abstract
We apply a combination of psi analysis (which probes the role of specific side-chain to side-chain contacts) and kinetic H/D amide isotope effects (which measures hydrogen-bond content), building upon previous studies using mutational phi analysis (which probes the energetic influence of side-chain...
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PMID: 18625237
PDF is available here.
PDF is available here.
Abstract
We describe the generation of highly purified engineered rHuPON1(K192) that protects against DZO exposure when injected into PON1(-/-) mice. The injected rHuPON1 is nontoxic, persists in serum for at least 2 days after injection, and provides protection against DZO exposures of at least three times...
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PMID: 18711144
PDF is available here.
PDF is available here.
Clinica Chimica Acta (395)1-2 2008
Abstract
The sensitivity of IgG-tTG detection with ELISA increased by protein A or protein G conjugates, whereas the specificity was reduced as compared with anti-IgG conjugate. The combined measurement of IgA-tTG and IgG-tTG still seems to be the optimal procedure when screening children for CD....
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PMID: 18514068
PDF is available here.
PDF is available here.