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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Streptavidin (0):
Recent article
Streptavidin
Abstract
A design of smart surfaces responsive to biochemical analytes is demonstrated in the example of mixed monolayers of biotin/fluorocarbon. The contact angle of aqueous solutions on such surfaces decreases upon streptavidin binding and can be used in detecting this protein. The specificity of the effect...
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PMID: 20161597
PDF is available here.
PDF is available here.
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Analytical Sciences (25)3 2009
Abstract
Proteins labeled with a luminescent lanthanide chelate, {{2,2',2'',2'''-{4'-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2':6',2''-terpyridine-6,6''-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTA-Eu(3+)),were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SD...
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PMID: 19276586
PDF is available here.
PDF is available here.
Abstract
We describe an efficient method for their rapid purification that relies on the capture of these receptors with streptavidin immobilized on agarose beads, and their subsequent release by enzymatic digestion with TEV protease. This method has been applied to several GPCRs belonging to the class A rho...
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PMID: 18835448
PDF is available here.
PDF is available here.
Abstract
The immobilization procedure to a biosensor surface has a major influence on the measurement results. To characterize the immobilization onto various biolayers, the interaction of DNA anti-thrombin aptamer with the protein thrombin was used as a model system. The aptamer was immobilized to a two-dim...
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PMID: 19377993
PDF is available here.
PDF is available here.
Abstract
Out of 535 cases, there were 481 (89.9%) cases of infiltrating ductal carcinoma with mean age of 48 years and mean tumour size of 4.4 cm. Tumour grade II was seen in 68% cases and lymph node metastases were present in 65% cases. HER-2/neu expression was seen in 31% cases, while ER and PR expression...
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PMID: 19208313
PDF is available here.
PDF is available here.
Methods in Molecular Biology (528)528 2009
Abstract
We describe a simple method for enrichment and separation of plasma membrane proteins, prior to their identification by tandem mass spectrometry. Cell surface proteins are labeled with biotin using a reagent which does not enter the cell, purified by differential centrifugation and then affinity cap...
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PMID: 19153690
PDF is available here.
PDF is available here.
Analytica Chimica Acta (632)2 2009
Abstract
I (cTnI) were analyzed using the EOC. A chemiluminescent signal proportional to the analyte concentration was produced by adding a luminogenic substrate to the tracer enzyme complexed with the analyte on the chip. The luminescent signal was detected in a dark chamber mounted with a cooled charge-cou...
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PMID: 19110101
PDF is available here.
PDF is available here.
Abstract
We report on porphyrin-based PCL for patterning poly(propylene sulfide) block copolymer films on gold substrates on the micrometer and submicrometer scales. We confirm chemical patterning with imaging ToF-SIMS and low-voltage SEM. Biomolecular patterning on micrometer and submicrometer scales is dem...
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PMID: 19113808
PDF is available here.
PDF is available here.
Abstract
We demonstrated that both nucleic acids hybridization and nucleic acids-protein interactions can be analyzed using perforated evanescent-field-coupled waveguide-mode nanobio-sensors....
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PMID: 19749276
PDF is available here.
PDF is available here.
Abstract
We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture ass...
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PMID: 19591045
PDF is available here.
PDF is available here.
Abstract
The conjugate of the residue of vitamin H (biotin, Bt) with the hormone of thyroid gland thyroxin (T4) was prepared by N-acylation of N-(3-aminopropyl) biotin amide with N-hydroxysuccinimide ester of N-acetyl thyroxin. The interactions of the Bt-T4 conjugate with one or simultaneously with two bindi...
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PMID: 19537169
PDF is available here.
PDF is available here.
Abstract
Zeptomole detector: A highly sensitive giant-magnetoresistive chip and FeCo nanoparticles can be used to linearly detect 600-4500 copies of streptavidin. Under unoptimized conditions, this system also detects human IL-6 with a sensitivity 13-times higher than that of standard ELISA techniques.
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PMID: 19288507
PDF is available here.
PDF is available here.
Abstract
We describe OXavidin, a product containing aldehyde groups, obtained by ligand-assisted sugar oxidation of avidin by sodium periodate. OXavidin chemically reacts with cellular and tissue proteins through Schiff's base formation thus residing in tissues for weeks while preserving the biotin binding c...
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PMID: 20130784
PDF is available here.
PDF is available here.
Abstract
We report here the first realization of an artificial branched DNA template where a single wall carbon nanotube is positioned with the necessary geometry of an individually gated field effect transistor.
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PMID: 19322421
PDF is available here.
PDF is available here.
Abstract
The effect of precipitation of complexes of streptavidin with biotinylated proteins under conditions of simple (according to Mancini) and double (according to Ouchterlony) radial diffusion in agar gel was studied. The position and form of precipitation lines depended primarily on the initial concent...
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PMID: 19235518
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (20)1 2009
Abstract
We utilize a combined approach of a fluorescence resonance energy transfer (FRET) quenched antibody with an "avidin chase" to increase TBR. Trastuzumab, a humanized monoclonal antibody against human epidermal growth factor receptor type 2 (HER2), was biotinylated and conjugated with the near-infrare...
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PMID: 19072537
PDF is available here.
PDF is available here.
Abstract
Oxidative posttranslational protein modifications occur as a normal process of cell biology and to a greater extent during pathogenic conditions. The detection and quantitation of protein oxidation has posed a continuing challenge to bioanalytical chemists because the products of oxidative protein d...
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PMID: 19378083
PDF is available here.
PDF is available here.
Abstract
We have demonstrated that using capillary electrophoresis (CE) as an enrichment step greatly improves the efficiency of the process. CE-SELEX is capable of isolating high-affinity aptamers in as little as 2-4 rounds of selection, shortening the process time from several weeks to as little as a few d...
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PMID: 19377982
PDF is available here.
PDF is available here.
Abstract
We describe a generalized in vitro selection experiment aiming at the enrichment of ssDNA aptamers using biotinylated target molecules. This procedure allows the application of streptavidin-biotin chemistry to separate bound from unbound DNA species during the selection process....
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PMID: 19377986
PDF is available here.
PDF is available here.
Abstract
We have shown that these linkage strategies can be used to successfully create quantum dot molecular beacons that can be used in DNA detection assays with high specificity....
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PMID: 19488712
PDF is available here.
PDF is available here.
Abstract
We present three complementary approaches for biotinylating proteins in vivo in Escherichia coli or in vitro using chemical or enzymatical reactions all of which can be scaled up to tag large numbers of proteins in parallel....
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PMID: 18988027
PDF is available here.
PDF is available here.
Abstract
This method aims at providing structural information on protein or nucleoprotein complexes by high-resolution electron microscopy. The objective is to promote the self-assembly of the macromolecules into two-dimensional crystals in order to use electron crystallography methods. When combined with ob...
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PMID: 19378176
PDF is available here.
PDF is available here.
Optics Express (16)26 2008
Abstract
We demonstrate a highly sensitive imaging method combined a terahertz time-domain spectroscopy and an interference effect for label-free protein detection on a polyvinylidene difluoride membrane. The method is based on terahertz time-domain spectroscopy and uses an interference effect. Biotin is lin...
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PMID: 19104643
PDF is available here.
PDF is available here.
Abstract
We report detection of streptavidin and clinically relevant levels of biotinylated monokine induced by interferon gamma (MIG) at physiological salt concentrations with AlGaN heterojunction field effect transistors (HFETs). The AlGaN HFETs are functionalized with a silane linker and analyte-specific...
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PMID: 18632260
PDF is available here.
PDF is available here.
Abstract
Attached bacteria inhabit the surfaces of many marine animals--a process that may play important roles in the survival and transport through aquatic systems. However, efficient detection of these bacteria has been problematic, especially small aquatic animals such as benthic harpacticoid copepod. Qu...
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PMID: 18718494
PDF is available here.
PDF is available here.