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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Bacterial Proteins (27):
- Streptavidin
- RNA Polymerase Sigma 54
- Exfoliatins
- Colicins
- Bacteriocins
- Integration Host Factors
- GroEL Protein
- Azurin
- AraC Transcription Factor
- Staphylococcal Protein A
- Periplasmic Proteins
- GroES Protein
- Botulinum Toxins
- Cell Wall Skeleton
- Penicillin-Binding Proteins
- Factor For Inversion Stimulation Protein
- Bacterial Transferrin Receptor Complex
- Tetanus Toxin
- Flavodoxin
- Ferredoxins
- Coagulase
- MutS DNA Mismatch-Binding Protein
- Luciferases, Bacterial
- Escherichia coli Proteins
- Flagellin
- Bacterial Outer Membrane Proteins
- Bacterial Capsules
Recent article
Bacterial Proteins
Abstract
We identify a previously unknown target of POA as the ribosomal protein S1 (RpsA), a vital protein involved in protein translation and the ribosome-sparing process of trans-translation. Three PZA-resistant clinical isolates without pncA mutation harbored RpsA mutations. RpsA overexpression conferred...
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PMID: 21835980
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Biomacromolecules (12)9 2011
Abstract
Polyhydroxyalkanoates (PHA) synthesis genes phbC and orfZ cloned from Ralstonia eutropha H16 were transformed into beta-oxidation weakened Pseudomonas putida KTOY08ΔGC, a mutant of P. putida KT2442. The recombinant P. putida strain termed KTHH06 was able to produce a short-chain-len...
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PMID: 21863836
PDF is available here.
PDF is available here.
Proteomics (11)17 2011
Abstract
Lactobacillus acidophilus NCFM is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits to the host. Lactitol is a synthetic sugar alcohol used as a sugar replacement in low calorie foods and selectively stimulating growth of L. aci...
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PMID: 21751373
PDF is available here.
PDF is available here.
Proteomics (11)18 2011
Abstract
This review covers progress in proteome research on Mycoplasma pneumoniae made over the last 5 years. This bacterium is one of the smallest known self-replicating bacteria. With fewer than 700 proposed proteins, it is well suited to a comprehensive proteome analysis. While all of the...
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PMID: 21751371
PDF is available here.
PDF is available here.
Proteomics (11)18 2011
Abstract
We present a simple, time- and cost-efficient approach to tackle the proteome of prokaryotic organisms. To obtain large data sets of complex biological experiments high-throughput and time- and cost-efficient methods still have to be developed and refined. In this study, we combined well-approved te...
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PMID: 21751368
PDF is available here.
PDF is available here.
Abstract
We used GeLC-MS/MS, in which proteins are separated using 1-DE and identified using nanoLC-MS/MS, to generate high-quality protein catalogs. To maximize the number of identifications, all data sets were searched against the target databases using two search engines, Mascot and Paragon. As a result,...
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PMID: 21615180
PDF is available here.
PDF is available here.
Abstract
We performed comparative proteomic analyses (2D-DIGE and MALDI-TOF/TOF and iTRAQ/SCX-LC-MS/MS) of cells at three different conditions: exponential, late stationary phase, and biofilms. These results were compared with alterations in the proteome resulting from exposure to a biofilm inhibitory compou...
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PMID: 21612302
PDF is available here.
PDF is available here.
PLoS Pathogens (7)7 2011
Abstract
We performed high throughput sequencing of cDNA generated from sRNA transcripts isolated from a strain ectopically expressing ToxT, the major transcriptional regulator within the virulence gene regulon. We compared this data set with ToxT binding sites determined by pulldown and deep sequencing to i...
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PMID: 21779167
PDF is available here.
PDF is available here.
Abstract
We have produced human CFTR in the prokaryotic expression host Lactococcus lactis. The full-length protein was detected in the membrane of the bacterium, but the yields were too low (< 0.1% of membrane proteins) for in vitro functional and structural characterization, and induction of the expression...
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PMID: 21742800
PDF is available here.
PDF is available here.
ISME Journal (5)7 2011
Abstract
Our results confirm the importance of pH and related geochemical factors in fine-tuning acidophilic microbial community structure and function at the species and strain level, and demonstrate the broad utility of proteomics in laboratory community studies....
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PMID: 21228889
PDF is available here.
PDF is available here.
Abstract
In Wistar rats, specific inhibitor UPS lactacystin induced degeneration of 24% of the dopaminergic neurons in the black substance. The work shows that a moderate weakening of the UPS function is characterized by an enhanced activity of the shaperon system. This process seems to resto...
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PMID: 21961290
PDF is available here.
PDF is available here.
Abstract
We provide an updated analysis of the evolutionary relationships between CRISPR-Cas systems and Cas proteins. Three major types of CRISPR-Cas system are delineated, with a further division into several subtypes and a few chimeric variants. Given the complexity of the genomic architectures and the ex...
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PMID: 21552286
PDF is available here.
PDF is available here.
Abstract
We make use of the random partial least squares regression technique (r-PLS) to trace connections between co-expressed genes in Mycobacterium tuberculosis using data downloaded from public microarray databases. We generated the overall topology of a microbial co-expression network with the exact com...
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PMID: 21514402
PDF is available here.
PDF is available here.
Proteomics (11)12 2011
Abstract
Small-colony variants (SCVs) of Staphylococcus aureus represent a slow-growing subpopulation causing chronic and relapsing infections due to their physiological adaptation on an intracellular lifestyle. In this first proteomic study on physiological changes associated with a natural,...
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PMID: 21595038
PDF is available here.
PDF is available here.
Abstract
We present a comprehensive, gel-free, and GeLC-MS/MS-based quantitative proteome profiling of S. aureus under this infection-relevant situation. (14)N(15)N metabolic labeling and three complementing approaches were combined for relative quantitative analyses of surface-associated proteins. The surfa...
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PMID: 21323324
PDF is available here.
PDF is available here.
Abstract
We compared the exoproteome of a nasal carrier strain of SA to a genetically similar noncarrier strain. Additionally, the carrier strain biofilm exoproteome was also compared against its planktonic counterpart. Using high throughput proteomics, it was observed that the carrier strain of SA secretes...
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PMID: 21338050
PDF is available here.
PDF is available here.
Proteomics (11)7 2011
Abstract
We report the subproteome reference maps of E. coli B REL606 by analyzing cytoplasmic, periplasmic, inner and outer membrane, and extracellular proteomes based on the genome information using experimental and computational approaches. Among the total of 3487 spots, 651 proteins including 410 non-red...
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PMID: 21337514
PDF is available here.
PDF is available here.
Journal of Proteomics (74)4 2011
Abstract
Obligately aerobic ammonia-oxidizing bacteria (AOB) like Nitrosomonas europaea play a pivotal role in the global nitrogen cycle. Although starvation tolerance is a key environmental adaptation, little is known about this response in AOB. The goal of these studies was to compare the c...
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PMID: 21172464
PDF is available here.
PDF is available here.
Abstract
We provide a structural explanation for the pathological properties of the complex formed between streptococcal M1 and human fibrinogen. A conformationally dynamic coiled-coil dimer of M1 was found to organize four fibrinogen molecules into a specific cross-like pattern. This pattern supported the c...
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PMID: 21475196
PDF is available here.
PDF is available here.
Abstract
Cell Host & Microbe (9)3 2011
Abstract
We find that HopZ1 physically interacts with the isoflavone biosynthesis enzyme, 2-hydroxyisoflavanone dehydratase (GmHID1). P. syringae infection induces gmhid1 expression and production of daidzein, a major soybean isoflavone. Silencing gmhid1 increases susceptibility to P. syringae infection, su...
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PMID: 21402357
PDF is available here.
PDF is available here.
Abstract
We have examined the relationship between host resistance and pathogen virulence using mice with a functional allele of the nramp-1 gene and lacking combinations of TLRs. Mice deficient in both TLR2 and TLR4 were highly susceptible to the intracellular bacterial pathogen Salmonella typhimurium, cons...
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PMID: 21376231
PDF is available here.
PDF is available here.
Cell Host & Microbe (9)2 2011
Abstract
We show that RIN4 phosphorylation activates RPM1. RIN4(142-176) is necessary and, with appropriate localization sequences, sufficient to support effector-triggered RPM1 activation, with the threonine residue at position 166 being critical. Phosphomimic substitutions at T166 cause effector-independen...
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PMID: 21320695
PDF is available here.
PDF is available here.
Proteomics (11)3 2011
Abstract
We mapped the surface-attached proteome of S. Typhimurium SL1344 grown in vitro under SPI-2-inducing conditions and identified 108 proteins; using secretion signal prediction software, 43% of proteins identified contained a signal sequence. Of these proteins, 13 were known secreted effector proteins...
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PMID: 21268266
PDF is available here.
PDF is available here.
Abstract
Many recent studies have revealed exquisite subcellular localization of proteins, DNA, and other molecules within bacterial cells, giving credence to the concept of prokaryotic anatomy. Common sites for localized components are the poles of rod-shaped cells, which are dynamically mod...
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PMID: 21095111
PDF is available here.
PDF is available here.
Abstract
These results show that secretin complexes contain previously unidentified large and flexible extra domains with a probable role in stabilization or assembly of type IV pili....
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PMID: 21304951
PDF is available here.
PDF is available here.
Abstract
We here investigated the contribution of ZFPM1 to human Th1/Th2 differentiation.
The cDNA of ZFPM1 was cloned and introduced into human cord blood CD4+ T cells by a lentiviral transduction system. Then, the expression of IL-4 and IFN-γ mRNA was determined by quantitative real-time R...
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PMID: 21646796
PDF is available here.
PDF is available here.
Abstract
The contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. However, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. Quorum sensing or cell-cell commu...
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PMID: 21031305
PDF is available here.
PDF is available here.
Abstract
We developed an in vitro protein-based assay system for the rapid detection and quantification of the furanosyl borate diester (BAI-2) subclass of autoinducer-2 (AI-2) QS molecules. The biosensor is based on the interaction of BAI-2 with the Vibrio harveyi QS receptor LuxP. Conformation changes asso...
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PMID: 21031302
PDF is available here.
PDF is available here.
Abstract
We describe the identification of the agr as a quorum sensing system in Staphylococcus aureus and the isolation of agr autoinducers and inhibitors by northern blotting, real-time RT-PCR, and β-lactamase reporter cells assays....
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PMID: 21031303
PDF is available here.
PDF is available here.
Abstract
The detection of bacterial signaling molecules in liquid or gaseous environments has been occurring in nature for billions of years. More recently, man-made materials and systems has also allowed for the detection of small molecules in liquid or gaseous environments. This chapter will outline some e...
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PMID: 21031306
PDF is available here.
PDF is available here.
Abstract
We describe a set of luminescent reporters, which could be used in high, medium or low throughput format, for the discovery and validation of agonists or antagonists of the Las QS system of Pseudomonas aeruginosa. These nearly isogenic reporters contain truncations or point mutations in the AHL bind...
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PMID: 21031308
PDF is available here.
PDF is available here.
Abstract
We describe a methodology to heterologously express, purify, and conduct in vitro characterization of several metalloforms of the AHL lactonase from Bacillus thuringiensis (AiiA). These procedures should be applicable to similar enzymes and will facilitate the production of more useful quorum-quench...
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PMID: 21031319
PDF is available here.
PDF is available here.
Abstract
Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affin...
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PMID: 20978976
PDF is available here.
PDF is available here.
Abstract
We have described the generation of quorum quenching monoclonal antibodies (mAbs) against acyl homoserine lactones (AHL) used by Pseudomonas aeruginosa as well as Staphylococcal autoinducing peptides (AIP). These mAbs suppressed QS signaling in bacteria and neutralized AHL-mediated cytotoxic effects...
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PMID: 21031321
PDF is available here.
PDF is available here.
Abstract
We describe the usage of reporter strains consisting of a lasB-gfp or rhlA-gfp fusion in P. aeruginosa for qualitative and quantitative evaluation of the inhibition of the two major QS pathways, monitored as reduced expression of green fluorescence. By the use of an in vitro flow cell system it is p...
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PMID: 21031317
PDF is available here.
PDF is available here.
Abstract
We have used the Mammalian Glycan Array to determine the specific affinities of these proteins. This was correlated with binding protein expression induced by different prebiotics including HMO. Half of the F1SBPs in B. infantis were determined to bind mammalian oligosaccharides. Their affinities in...
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PMID: 21423604
PDF is available here.
PDF is available here.
Abstract
We explored the global transcriptional regulation by CovR in S. mutans. Comparison of the transcriptome profiles of the wild-type strain UA159 with its isogenic covR deleted strain IBS10 indicated that at least 128 genes (∼6.5% of the genome) were differentially regulated. Among these genes, 69 we...
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PMID: 21655290
PDF is available here.
PDF is available here.
Abstract
We determined the complete genomic sequence of H. parasuis SH0165, a highly virulent strain of serovar 5, which was isolated from a hog pen in North China. The single circular chromosome was 2,269,156 base pairs in length and contained 2,031 protein-coding genes. Together with the full spectrum of g...
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PMID: 21611187
PDF is available here.
PDF is available here.
Abstract
We previously demonstrated that, in response to B. pseudomallei infection of macrophage cell line RAW 264.7, a subset of bacteria co-localized with the autophagy marker protein, microtubule-associated protein light chain 3 (LC3), implicating autophagy in host cell defence against infection. Recent r...
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PMID: 21412437
PDF is available here.
PDF is available here.
Abstract
We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability. Transmission electron micrographs revealed cell wall thickening and aberrant division septa in the absence of WalKR, suggesting its...
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PMID: 21386961
PDF is available here.
PDF is available here.
Abstract
We present complete genome sequences for both taxa, with analyses involving other species of Streptococcus but focusing on adaptation in the SD species group. We found little evidence for enrichment in biochemical categories of genes carried by each SD strain, however, differences in the virulence g...
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PMID: 21282711
PDF is available here.
PDF is available here.
Abstract
We demonstrate that Hfq, an RNA-binding protein, which facilitates interactions between sRNAs and their mRNA targets, is critical for K. pneumoniae virulence. A K. pneumoniae mutant lacking hfq (Δhfq) failed to disseminate into extra-intestinal organs and was attenuated on induction of a systemic i...
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PMID: 21779404
PDF is available here.
PDF is available here.
Abstract
We present a method for structure-based virtual screening that is based on the comprehensive prediction of ligand binding sites on a protein model and automated construction of a ligand-receptor interaction map. Pharmacophoric features of the map are clustered and transformed in a correlation vector...
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PMID: 21483848
PDF is available here.
PDF is available here.
Abstract
We report the regulatory response of C. crescentus to carbon starvation, based on combined high-throughput proteome and transcriptome analyses. Our results identify cell cycle changes in gene expression in response to carbon starvation that involve the prominent role of the FixK FNR/CAP family trans...
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PMID: 21494595
PDF is available here.
PDF is available here.
Abstract
We characterize how the O. carboxidovorans proteome adapts to different lifestyles of chemolithoautotrophy and heterotrophy. Fatty acid methyl ester (FAME) analysis of O. carboxidovorans grown with acetate or with syngas showed that the bacterium changes membrane fatty acid composition. Quantitative...
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PMID: 21386900
PDF is available here.
PDF is available here.
Abstract
We have utilized sensitive proteomic approaches to examine the impact of diurnal rhythms on the protein complement in Cyanothece 51142. We found that 250 proteins accounting for ∼5% of the predicted ORFs from the Cyanothece 51142 genome and 20% of proteins detected under alternating light/dark con...
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PMID: 21364985
PDF is available here.
PDF is available here.
Abstract
These results provided a fundamental understanding of carbohydrate metabolism in alkaliphiles....
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PMID: 21253875
PDF is available here.
PDF is available here.
Metallomics (3)1 2011
Abstract
We treat the changes in copper proteins in historically the same increasingly oxidising environmental conditions. The study is a bioinformatic analysis of the types and the numbers of copper domains of proteins from 435 DNA sequences of a wide range of organisms available in NCBI, using the method d...
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PMID: 21085721
PDF is available here.
PDF is available here.
Epidemiology and Infection (139)1 2011
Abstract
Our data suggest that MIRU genotype 223325173533 of the Beijing family is associated with MDR and increased transmissibility....
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PMID: 20429968
PDF is available here.
PDF is available here.
Abstract
Journal of Biological Chemistry (285)52 2010
Abstract
We proposed that FtsY requires functional interaction with inner membrane lipids at a late stage of the signal recognition particle pathway. In addition, an essential lipid-binding α-helix was identified in FtsY of various origins. Theoretical considerations and in vitro studies have suggested that...
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PMID: 20956528
PDF is available here.
PDF is available here.
Chemmedchem (5)12 2010
Abstract
We report the discovery of highly potent and selective mPTPB inhibitors using a novel, double Click chemistry strategy. The most potent mPTPB inhibitor from this approach possesses a K(i) value of 160 nM and a >25-fold selectivity for mPTPB over 19 other protein tyrosine phosphatases (PTBs). Molec...
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PMID: 20957718
PDF is available here.
PDF is available here.
Abstract
Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of...
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PMID: 19214390
PDF is available here.
PDF is available here.
Abstract
Abstract
We demonstrate that the resistance enzyme ANT(4') from Staphylococcus aureus is highly substrate and cosubstrate promiscuous. We show that deoxy-ribonucleotide triphosphates (dNTPs) are better cosubstrates than NTPs. We demonstrate that the position of the triphosphate group (5' and not 3') on the r...
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PMID: 21040710
PDF is available here.
PDF is available here.
Abstract
RNA-based pathways that regulate protein expression are much more widespread than previously thought. Regulatory RNAs, including 5' and 3' untranslated regions next to the coding sequence, cis-acting antisense RNAs and trans-acting small non-coding RNAs, are effective regulatory mole...
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PMID: 21079634
PDF is available here.
PDF is available here.
Abstract
We report a metagenomic analysis of a healthy human plaque sample using a combination of second-generation sequencing platforms. A total of 860 million base pairs of non-human sequences were generated. Various analysis tools revealed the presence of 12 well-characterized phyla, members of the TM-7 a...
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PMID: 21040513
PDF is available here.
PDF is available here.
Abstract
This case-controlled study examined clinical and microbiological parameters in Brazilian children and adolescents receiving orthodontic treatment using fixed orthodontic appliances or removable orthodontic appliances.
The plaque index, gingival index, number of decaye...
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PMID: 21344829
PDF is available here.
PDF is available here.
Abstract
A three dimensional model was developed for Cry10Aa protein sequence of B. thuringiensis LDC-9 and B. thuringiensis israelensis that has not been solved empirically by X-ray crystallography or NMR. Homology modeling was employed for the structure prediction using Cry2Aa as template protein, a high-r...
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PMID: 20919752
PDF is available here.
PDF is available here.