Abstract
Borreliosis has been widely recognized in Switzerland and is often used in unclear cases with non-specific symptoms. Two illustrative cases should emphasize the current options for diagnosis and therapy of neuroborreliosis. The keystones of the diagnostic instruments are a history wi...
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PMID: 21563099
PDF is available here.
Abstract
In up to half of all cases of community-acquired pneumonia (CAP), no pathogen can be identified with conventional diagnostic methods. The most common identified causative agent is Streptococcus pneumoniae. In this study, pneumococcal antibody responses during CAP were analyzed to est...
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PMID: 21367978
PDF is available here.
Abstract
In recent years, better diagnostics for tuberculosis (TB) has received increasing attention, especially the diagnosis of tuberculous pleural effusion, which is difficult and at present the main tool in TPE diagnostic is pleural effusion smear and culture, but unfortunately, sensitivi...
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PMID: 21612137
PDF is available here.
Abstract
We have evaluated the immunostimulatory role of the highly-conserved outer membrane lipoprotein P6 from non-typeable Haemophilus influenzae (NTHI) to determine whether the presence of the lipid motif plays a critical role on its immunogenicity. We undertook a systematic analysis of the role that the...
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PMID: 21611194
PDF is available here.
Abstract
These results highlight the important risk factors for Brucella spp. infection in smallholder herds. Thus, brucellosis control programmes which take these factors into consideration will be beneficial....
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PMID: 20478082
PDF is available here.
Abstract
Salmonella Paratyphi A (SPA) is rapidly becoming a common cause of enteric fever in South East Asia. A large outbreak of SPA occurred in a boarding middle school in China in 2004. There were 394 suspected cases; 95·5% were students. The highest incidence was in the youngest children (7th grade). Fo...
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PMID: 20800009
PDF is available here.
S J Olsen,
S Thamthitiwat,
S Chantra,
M Chittaganpitch,
A M Fry,
J M Simmerman,
H C Baggett,
T C T Peret,
D Erdman,
R Benson,
D Talkington,
L Thacker,
M L Tondella,
J Winchell,
B Fields,
W L Nicholson,
S Maloney,
L F Peruski,
K Ungchusak,
P Sawanpanyalert and
S F Dowell
Abstract
Although pneumonia is a leading cause of death from infectious disease worldwide, comprehensive information about its causes and incidence in low- and middle-income countries is lacking. Active surveillance of hospitalized patients with pneumonia is ongoing in Thailand. Consenting patients are teste...
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PMID: 20353622
PDF is available here.
Abstract
We enrolled adults undergoing chest radiography at three Indian Health Service clinics in the Southwestern United States and collected acute and convalescent serum for measurement of PsaA and PspA titres and urine for pneumococcal antigen detection. Blood and sputum cultures were obtained at the dis...
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PMID: 20334727
PDF is available here.
Abstract
We collected respiratory tract samples of adult outpatients with symptoms of pneumonia in a German nationwide network and detected M. pneumoniae by real-time PCR in 140 specimens. The strains were typed by sequencing and demonstrated the circulation of subtypes 1 and 2 and variants 2a and 2b. The ov...
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PMID: 20334729
PDF is available here.
Abstract
We report a new testing method named colloidal gold-immunochromatography assay (GICA) to detect syphilis instead of fluorescent treponemal antibody-absorption (FTA-Abs). Syphilis-specific immunoglobulin G (IgG) antibody was detected with GICA established on syphilis-specific recombinant proteins, TP...
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PMID: 20846810
PDF is available here.
Abstract
A modified, non-species dependent ELISA was performed to detect antibodies in sera of Burkholderia pseudomallei-immunized goats using protein G- or protein A-peroxidase conjugates. The rise of antibody titers during the immunization period exhibited corresponding results by modified ELISA comparison...
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PMID: 20849887
PDF is available here.
Wenyan Hong,
Lihua Huang,
Haoran Wang,
Jianfeng Qu,
Zhaobiao Guo,
Chengke Xie,
Ziwen Zhu,
Youbao Zhang,
Zongmin Du,
Yanfeng Yan,
Yan Zheng,
Huijie Huang,
Ruifu Yang and
Lei Zhou
Abstract
In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immun...
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PMID: 20801166
PDF is available here.
Abstract
In this paper, a sensitive immunoassay method was proposed for Listeria monocytogenes detection by using highly fluorescent bioconjugated nanoparticles probe. (FITC-IgG)-doped fluorescent silica nanoparticles (fsNPs) firstly were synthesized by a microemulsion method and characterized by TEM and flu...
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PMID: 20807555
PDF is available here.
Abstract
The basis for efficacy of the recombinant botulinum vaccine, serotypes A and B (rBV A/B) is that neutralizing antibodies induced by vaccination bind to botulinum neurotoxin complex serotype A, subtype A1 (BoNT/A1) and serotype B, subtype B1 (BoNT/B1) and prevent their actions at cholinergic neurons....
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PMID: 20816903
PDF is available here.
Abstract
We found a higher number of H. parasuis strains (16 strains) within a given farm than previously thought. Overall, more H. parasuis diversity was found in piglets from non-vaccinated sows than in those from vaccinated sows. These results indicate that vaccination of sows in a farm delays the coloniz...
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PMID: 20447775
PDF is available here.
Abstract
The combination of optimal multiple antigens to detect anti-MTB antibody responses increased the sensitivity and specificity. Therefore, detection of anti-MTB antibody responses with multiple antigens may be valuable in the clinical diagnosis of TB patients.
Copyright 2010. Published by Elsevier B.V...
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PMID: 20599865
PDF is available here.
Abstract
We have developed a single 17-residue peptide immunogen that generates antibodies that target the receptor-binding domain of the type IV pilus of more than one strain of P. aeruginosa. Using the receptor-binding domain sequence, of native strain PAO as a template, we have systematically changed up...
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PMID: 20807222
PDF is available here.
Abstract
The objective of the present study was to construct and assess the immunogenicity of recombinant adenovirus vectors expressing the HMW1, HMW2, or Hia protein of nontypeable Haemophilus influenzae (NTHi). These proteins are critical adhesins and potential protective antigens expressed...
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PMID: 20685934
PDF is available here.
Abstract
We investigated the presence of Chlamydophila pneumoniae antibodies in 125 patients with cardiovascular disease and in 128 controls. C. pneumoniae antibodies were measured by microimmunofluorescence assay. A significantly high prevalence of IgG C. pneumoniae antibodies at titre > or = 8 was found in...
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PMID: 21213597
PDF is available here.
Abstract
Our results indicated that T. forsythia and P. gingivalis exhibit virulence, but not virulence synergy, resulting in the immuno-inflammatory responses and lack of humoral immune protection during periodontitis in rats.
© 2010 John Wiley & Sons A/S....
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PMID: 20846155
PDF is available here.
Abstract
We present the case of a previous healthy child, who ultimately succumbed to H. influenzae type B meningitis where multiple factors were likely responsible for his acquisition of the disease....
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PMID: 20930601
PDF is available here.
Abstract
Human leptospirosis is a zoonotic disease of global importance that causes significant morbidity and mortality, particularly in developing nations. In this review, the history, epidemiology, transmission, clinical presentation and treatment of this disease, and its impact in Australia, are discussed...
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PMID: 21092392
PDF is available here.
Abstract
We report the case of a 40-year-old man with underlying alcoholic liver disease who presented with prolonged fever, jaundice and liver failure associated with Coxiella burnetii infection. After diagnosis and appropriate antibiotic treatment, the patient made a complete recovery. We describe aspects...
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PMID: 20685010
PDF is available here.
Marie Moinet,
Christine Fournier-Chambrillon,
Geneviève André-Fontaine,
Stéphane Aulagnier,
Alain Mesplède,
Béatrice Blanchard,
Véronique Descarsin,
Philippe Dumas,
Yann Dumas,
Christophe Coïc,
Laurent Couzi and
Pascal Fournier
Abstract
We conducted a survey of antibody prevalence and renal carriage of pathogenic leptospira (Leptospira interrogans sensu lato) using serum and kidney samples collected from 1990 to 2007 from several free-ranging small carnivores and farmed American mink (Mustela vison) in southwestern France. An indir...
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PMID: 20966265
PDF is available here.
Abstract
We conducted a study to determine the seroprevalence of Brucella spp. in the Kafue lechwe and to evaluate serologic tests for detection of Brucella spp. antibodies in lechwe. The Rose Bengal Test (RBT), competitive enzyme-linked immunosorbent assay (cELISA), and fluorescence polarization assay (FPA)...
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PMID: 20966257
PDF is available here.
Abstract
A serologic survey of anti-Brucella and antileptospiral antibodies was conducted on 147 adult, female New Zealand sea lions (Phocarctos hookeri). Most sea lions (n=138) were sampled at Sandy Bay, Enderby Island, Auckland Islands (50°30'S, 166°17'E), January 2000-March 2005. Nine were sampled at Ot...
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PMID: 20966283
PDF is available here.
Vaccine (28 Suppl 5) 2010
Abstract
The first report in this chapter describes the development of a killed composite vaccine. This killed vaccine is non-infectious to humans, other animals, and the environment. The vaccine has low reactivity, is non-abortive, and does not induce pathomorphological alterations to the organs of vaccinat...
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PMID: 20850688
PDF is available here.
Abstract
A six-year-old boy with high-grade fever and abdominal pain in the epigastric region was examined with ultrasonogram of the abdomen. Hematology-cell analysis, serology (Widal test), urine analysis, and blood cultures were also performed. The ultrasonogram was helpful for the identification of multip...
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PMID: 21045376
PDF is available here.
Abstract
Two Japanese travelers from Bali were diagnosed with murine typhus in Japan during the same period. Although one had only mild illness, the other experienced liver and kidney dysfunction. Murine typhus may be missed not only in endemic areas around the world, but also in travelers, especially those...
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PMID: 20920060
PDF is available here.
Abstract
Maternal antibodies, transported through the placenta during pregnancy, contribute to the protection of infants from infectious diseases during the first months of life. The aim of this study was to measure the concentration of antibodies against several vaccine-preventable diseases in paired matern...
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PMID: 20803841
PDF is available here.
Abstract
We attempted to develop a new specific antibody detection method for discriminating infection state from colonization state in hospitalized immunocompromised patients with a positive sputum culture for Pseudomonas aeruginosa. Serum samples from 65 patients with P. aeruginosa in sputum culture (total...
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PMID: 20538018
PDF is available here.
Abstract
We have previously demonstrated the efficacy of recombinant chlamydial protease-like activity factor (rCPAF; a secreted chlamydial protein) in inducing antigen-specific CD4+ T cell/gamma interferon (IFN-gamma)-mediated but not antibody-mediated chlamydial clearance and reduction of upper genital tra...
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PMID: 20605976
PDF is available here.
Abstract
We demonstrate that the biosensor was able to detect SEB at concentrations as low as 5 ng/mL at a signal to baseline (S/B) ratio of 2. Such measurements were performed on the chip in wet conditions. The actuation of the chip by SEB is immediate, permitting real-time signal measurements. In addition...
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PMID: 20447819
PDF is available here.
Abstract
We found no statistically significant difference between O and non-O individuals either in the frequency distributions of the fold increase or in the postvaccination increase in geometric mean titer compared to the baseline. Further, in contrast to the earlier observation that the O allele frequency...
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PMID: 20554804
PDF is available here.
Abstract
We utilized this relevant animal model to investigate the safety and immunogenicity of a triple mutant of SEB carrying the amino acid changes L45R, Y89A, and Y94A. This recombinant mutant SEB (rmSEB) did not possess superantigen activity in pig lymphocyte cultures. Furthermore, rmSEB was unable to c...
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PMID: 20554806
PDF is available here.
Abstract
There is mounting evidence that innate and adaptive immunity are critical for periodontal disease-mediated bone resorption. These studies examined the role of B and CD4 T cells in adaptive immunity of rats infected with Aggregatibacter actinomycetemcomitans (Aa). Sprague-Dawley male...
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PMID: 20618701
PDF is available here.
V Bakthavatchalu,
A Meka,
S Sathishkumar,
M C Lopez,
R K Verma,
S M Wallet,
I Bhattacharyya,
B F Boyce,
J J Mans,
R J Lamont,
H V Baker,
J L Ebersole and
L Kesavalu
Abstract
We examined changes in the host transcriptional profiles during a T. denticola infection using a murine calvarial model of inflammation and bone resorption. T. denticola was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and the c...
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PMID: 20618700
PDF is available here.
Abstract
A prospective cohort observational study was conducted to investigate the seroprevalence of Anaplasma marginale in Iowa feedlots and its association with morbidity, mortality, and treatment costs. Blood samples were taken from 659 calves from 31 consigners at processing and classified as seropositiv...
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PMID: 21037886
PDF is available here.
Abstract
We report the generation of the first monoclonal antibody that specifically binds to the polysaccharide chitosan. Mice were immunized with a mixture of chitosans, and hybridoma clones were screened for specific binders resulting in the isolation of a single clone secreting a chitosan-specific IgM, m...
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PMID: 20798578
PDF is available here.
Abstract
Two types of silver nanoparticles were activated by specific sorption of biomolecules for the detection of Escherichia coli. The capture of this bacterium was performed using polyclonal antibodies (anti-E. coli) biosorbed onto nanospheres or nanorice through a protein-A layer. The bacterial detectio...
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PMID: 19649746
PDF is available here.
Abstract
We assumed that a rare HCMV and M. pneumoniae coinfection was the aetiology of the fulminant pneumonia. Under broad antibiotic and antiviral treatment, the situation of the patient improved only very slowly....
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PMID: 20466842
PDF is available here.
Abstract
Viral vectors based on influenza virus, rabies virus (RV), and vaccinia virus (VV) were used to express large polypeptide segments derived from the Bacillus anthracis protective antigen (PA). For the infectious influenza virus vector and recombinant VV constructs, the receptor binding domain (RBD or...
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PMID: 20504926
PDF is available here.
Abstract
We describe a subunit vaccine against ExPEC which is based on immunodominant epitopes of the virulence-associated ExPEC proteins FyuA, IroN, ChuA, IreA, Iha, and Usp. Using a novel approach of computer-aided design, two completely artificial genes were created, both encoding eight peptide domains de...
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PMID: 20498257
PDF is available here.
Abstract
We developed an infection model in murine neonates using the gastroenteric pathogen Yersinia enterocolitica. Here, we report that oral infection of neonatal mice with low doses of virulent Y. enterocolitica leads to vigorous intestinal and systemic adaptive immunity. Y. enterocolitica infection prom...
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PMID: 20515925
PDF is available here.
Abstract
We sought to investigate novel approaches that would enable detection of vaccine specific IgA memory B cells. We demonstrate that stimulation of human peripheral blood mononuclear cells in vitro with immunostimulatory DNA in combination with B cell-activating factor (BAFF) and IL-15 promotes differe...
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PMID: 20547203
PDF is available here.
Abstract
We characterized the levels of antibodies against protective antigen and found that over half of anthrax vaccinees had low serum levels of in vitro toxin neutralization capacity. Using solid-phase epitope mapping and confirmatory assays, we identified several neutralization-associated humoral epitop...
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PMID: 20533877
PDF is available here.
Abstract
This study evaluated the occurrence of granulomatous lymphadenitis and its association with Actinobacillus spp. in 151 653 slaughtered pigs. Markedly enlarged pulmonary hilar, mediastinal, mandibular or hepatic lymph nodes were detected in 6 castrated males. The cut surfaces showed multifocal yellow...
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PMID: 20885825
PDF is available here.
Carl T D'Angio,
Roy J Heyne,
T Michael O'Shea,
Robert L Schelonka,
Seetha Shankaran,
Shahnaz Duara,
Ronald N Goldberg,
Barbara J Stoll,
Krisa P Van Meurs,
Betty R Vohr,
Abhik Das,
Lei Li,
Robert L Burton,
Betty Hastings,
Dale L Phelps,
Pablo J Sanchez,
Waldemar A Carlo,
David K Stevenson,
Rosemary D Higgins and
NICHD Neonatal Research Network
Abstract
We hypothesized that the frequency of postvaccine antibody concentrations > or =0.15 microg/mL would vary directly with birth weight.
This was a multicenter observational study. Infants 401 to 1500 g birth weight and <32 0/7 weeks gestation, stratified by birth weight, were enrolled from 9 National...
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PMID: 20234331
PDF is available here.
Abstract
Gopher tortoise (Gopherus polyphemus) populations on four tracts of public lands in northern and central Florida were studied from 1998 to 2001 to assess the effects of mycoplasmal upper respiratory tract disease (URTD). Adult gopher tortoises (n=205) were marked for identification, serum and nasal...
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PMID: 20688675
PDF is available here.
Abstract
We report on the presence of specific antibodies to Brucella spp. and Yersinia enterocolitica in polar bears (Ursus maritimus) from northern Alaska (southern Beaufort Sea) during 2003-2006. Based on numerous known stressors (e.g., climate change and loss of sea ice habitat, contaminants), there is i...
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PMID: 20688674
PDF is available here.
Abstract
Whole-blood samples were obtained from 214 white-tailed deer (Odocoileus virginianus) representing 44 sites in Connecticut (USA) during 1992, 1993, 1996, 1999, and 2000 through 2006. Sera were analyzed for total antibodies to whole-cell or recombinant antigens of Borrelia burgdorferi sensu stricto a...
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PMID: 20688684
PDF is available here.
Abstract
We studied free living macaws and parrots in the Tambopata National Reserve in the Peruvian Amazon and semicaptive domestic fowl in human settlements adjacent to the reserve. In 1992-1993, large macaws (Aras spp.) that were serologically positive for Salmonella Pullorum were released into this reser...
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PMID: 20688677
PDF is available here.
Abstract
We report on a retrospective analysis of clinical manifestations, lab investigations and outcome of patients admitted to our hospital from January 2009 to December 2009 with scrub typhus. The Weil-Felix test was the serological method used to diagnose scrub typhus. A total of 29 patients were diagno...
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PMID: 20555045
PDF is available here.
Abstract
We assessed the presence of these hemoparasites in the blood of Brazilian wild captive felids. Of the 72 animals tested, 5 (7%) were seropositive for the E. canis antigen, and 11 (15%) were positive for E. canis DNA sequences. We also performed sequence alignment to establish the identity of the par...
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PMID: 20688716
PDF is available here.